Addgene

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pHH0103_GNA13_G-alpha

Plasmid

#109913

Purpose

Protein expression and purification of GNA13_G-alpha

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

GNA13_G-alpha (GNA13 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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actc1b-mKate2-rab8a

Plasmid

#109657

Purpose

Muscle specific zebrafish expression of mKate2 fused to rab8a

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

rab8a (rab8a Zebrafish)

Use

Zebrafish plasmids

Tags

mKate2

Expression

Mutation

Promoter

Available since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pHH0103_PABPC1_RRM

Plasmid

#109870

Purpose

Protein expression and purification of PABPC1_RRM

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

PABPC1_RRM (PABPC1 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pHH0103_ESR2_Hormone-recep

Plasmid

#109907

Purpose

Protein expression and purification of ESR2_Hormone-recep

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

ESR2_Hormone-recep (ESR2 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pHH0103_SUB1_PC4

Plasmid

#109874

Purpose

Protein expression and purification of SUB1_PC4

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

SUB1_PC4 (SUB1 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 30, 2020

Availability

Academic Institutions and Nonprofits only

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pBCSK_HIS3_II

Plasmid

#109059

Purpose

yeast shuttle vector with deletable CEN6-ARSH4 cassette. For teaching purposes.

Depositor

Colin Logie

Insert

HIS3 (HIS3 Zygosaccharomyces rouxii, Budding Yeast)

Use

Cre/Lox

Tags

Expression

Bacterial and Yeast

Mutation

Promoter

HIS3

Available since

Aug. 1, 2018

Availability

Academic Institutions and Nonprofits only

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pWL1 (mANGPTL8-V5-His)

Plasmid

#109113

Purpose

Expresses V5 and His-tagged mouse ANGPTL8 in mammalian cells

Depositor

Brandon Davies

Article

Chi et al Mol Metab. 2017 Oct;6(10):1137-1149. doi

Insert

ANGPTL8 (Angptl8 Mouse)

Use

Tags

6x His and V5

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 21, 2018

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA3 RL3Am

Plasmid

#109364

Purpose

Human rod opsin chimera with intracellular loop 3 from Amphioxus opsin 1 with 1D4 tag

Depositor

Robert Lucas

Article

Ballister et al BMC Biol. 2018 Jan 16;16(1):10. doi: 10.

Insert

Rod opsin chimera with Amphioxus Opsin1 intracellular Loop 3 (RHO Branchiostoma belcheri, Human)

Use

Tags

1D4

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 10, 2018

Availability

Academic Institutions and Nonprofits only

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NK590

Plasmid

#109084

Purpose

Reporter gene expression in S. rosetta

Depositor

Nicole King

Article

Booth et al Mol Biol Cell. 2018 Dec 1;29(25):3026-30

Insert

mPapaya

Use

Choanoflagellate expression

Tags

Expression

Mutation

Promoter

Available since

May 14, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_GNAT1_G-alpha

Plasmid

#109946

Purpose

Protein expression and purification of GNAT1_G-alpha

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

GNAT1_G-alpha (GNAT1 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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p3E-RhoAa

Plasmid

#109577

Purpose

Multisite gateway vector for 3' tagging with RhoAa

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

RhoAa (rhoaa Zebrafish)

Use

Zebrafish plasmids

Tags

Expression

Mutation

Promoter

Available since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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[pSK497] pLJC5 Flag-Depdc5(AB)

Plasmid

#109341

Purpose

Lenti-viral expression of Flag-tagged Depdc5 mutant AB

Depositor

David Sabatini, Kuang Shen

Article

Shen et al Nature. 2018 Apr 5;556(7699):64-69. doi:

Insert

Depdc5 (DEPDC5 Human)

Use

Lentiviral

Tags

FLAG

Expression

Mutation

DIYGD(185-189)GSGSG;EQPLH(371-375)GSGSG

Promoter

Available since

July 24, 2018

Availability

Academic Institutions and Nonprofits only

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p3E-DysF

Plasmid

#109544

Purpose

Multisite gateway vector for 3' tagging with Dysferlin

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

Dysferlin (dysf Zebrafish)

Use

Zebrafish plasmids

Tags

Expression

Mutation

Please see Depositor Comments

Promoter

Available since

March 6, 2020

Availability

Academic Institutions and Nonprofits only

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pLG1-puro-sgATL3-2

Plasmid

#109010

Purpose

CRISPRi knockdown of targeted gene (to be used with Addgene #102244 or other dCas9-KRAB constructs)

Depositor

Jacob Corn

Article

Liang et al J Cell Biol. 2018 Aug 24. pii: jcb.20180

Insert

ATL3-sgRNA #2 (ATL3 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

mU6 promoter

Available since

Aug. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_EGFR_Recep-L

Plasmid

#109920

Purpose

Protein expression and purification of EGFR_Recep-L

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

EGFR_Recep-L

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pLG1-puro-sgATL2-1

Plasmid

#109008

Purpose

CRISPRi knockdown of targeted gene (to be used with Addgene #102244 or other dCas9-KRAB constructs)

Depositor

Jacob Corn

Article

Liang et al J Cell Biol. 2018 Aug 24. pii: jcb.20180

Insert

ATL2-sgRNA #1 (ATL2 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

mU6 promoter

Available since

Aug. 22, 2018

Availability

Academic Institutions and Nonprofits only

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B9L-BirA*

Plasmid

#109146

Purpose

To generate stable cell lines expressing B9L-BirA* for BioID proximity labelling through co-transfection with pOG44 in Flp-In T-REx host cells

Depositor

Mariann Bienz

Article

van Tienen et al Elife. 2017 Mar 15;6. doi: 10.7554/eLife

Insert

B9L (Bcl9l Mouse)

Use

Tags

BirA(R118G) and Flag

Expression

Mammalian

Mutation

Promoter

CMV/TetO2

Available since

July 2, 2018

Availability

Academic Institutions and Nonprofits only

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pJCSUP35(123-254)

Plasmid

#1090

Depositor

Susan Lindquist

Article

Glover et al Cell 1997 May 30;89(5):811-9.

Insert

SUP35 (SUP35 Budding Yeast)

Use

Tags

His

Expression

Bacterial

Mutation

amino acids 123 - 254

Promoter

Available since

Jan. 5, 2006

Availability

Academic Institutions and Nonprofits only
pSUM36-MCS2-myc

Plasmid

#109379

Purpose

Shuttle vector for high level expression in mycobacteria.

Depositor

Nicolai van Oers

Article

Eitson et al Appl Environ Microbiol. 2012 Oct;78(19):

Insert

MCS2-Myc

Use

Tags

Myc

Expression

Bacterial

Mutation

Promoter

Kanamycin

Available since

Sept. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pU6-crRNA(SOD1)

Plasmid

#109321

Purpose

AAV vector expressing crRNA for AsCpf1 (RR variant) targeting human SOD1

Depositor

Feng Zhang

Article

Tekin et al Nat Biomed Eng. 2018 Jul;2(7):540-554. d

Insert

mCherry-KASH (SOD1 Human)

Use

AAV

Tags

Expression

Mutation

Promoter

hSyn1

Available since

April 30, 2018

Availability

Academic Institutions and Nonprofits only

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