We narrowed to 12,150 results for: 112
-
Plasmid#112290PurposeFluorescently labels human YAP1-2gamma isoform (504 aa) WW mutant. WQDP (199-202) in the first WW domain was changed to AQDA, and the WLDP (258-261) of the second WW domain was changed to ALDADepositorInsertEYFP-YAP1 (YAP1 Human)
UseLentiviralMutationHuman YAP1-2gamma with mutations in the WW domain…PromoterEF-1 alphaAvailable SinceMay 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSopB2xHA
Plasmid#112513PurposePsopB driven sopB-2xHA and sigEDepositorInsertsopB-2xHA/sigE operon
Tags2xHAExpressionBacterialMutationSopB C-terminal 2xHAPromoterPsopBAvailable SinceJuly 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBCL6.1.0-gDNA
Plasmid#112485PurposeCRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor BCL6DepositorAvailable SinceDec. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJW1667
Plasmid#112041PurposeCEN/ARS plasmid for GAL-inducible ERG5-V5-TEV-EGFP expression marked with URADepositorInsertERG5
TagsV5-TEV-EGFPExpressionYeastPromoterGAL1Available SinceDec. 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
mScarlet-I-T2A-LSS-SGFP2
Plasmid#112946PurposeProduces equimolar levels of two fluorescent proteins. It can be used to measure practical brightness and as a negative control for FRETDepositorInsertmScarlet-I and LSS-SGFP2
ExpressionMammalianPromoterCMVAvailable SinceSept. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLJM1 FRB-myc-RagC
Plasmid#112741Purposeexpress FRB-myc-RagCDepositorAvailable SinceOct. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28a_6HT-TBP6.7(R49A)
Plasmid#112726PurposeArg49 of TBP6.7 and other TBPs is the only arginine that was lab-evolved from wild-type U1A to play a significant role in TAR RNA recognition. Mutation results in ~230-fold reduction in Kd.DepositorInsert6His-TEV-TBP6.7(R49A)
Tags6His-TEVExpressionBacterialMutationR49APromoterT7Available SinceAug. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJW1660
Plasmid#112034PurposeC-terminal tag with AID*(AtIAA17_71-114)-HA marked with LEUDepositorInsertIAA17
TagsHAExpressionYeastMutationonly amino acids 71-114PromoterNoneAvailable SinceAug. 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDEST-APIC-LexAGAD
Plasmid#112807Purposegateway cloning destination vector to express enhancer driven LexAGAD in DrosophilaDepositorTypeEmpty backboneExpressionBacterialAvailable SinceSept. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
p415 Gcs1
Plasmid#112646PurposeExpression S.cerevisiaeDepositorInsertGcs1
ExpressionYeastPromoterMet25Available SinceSept. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
LifeAct-LSS-SGFP2-N1
Plasmid#112938PurposeVisualization of F-actinDepositorInsertLifeAct
TagsLSS-SGFP2ExpressionMammalianPromoterCMVAvailable SinceSept. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGEX-2T-NM
Plasmid#1127DepositorAvailable SinceMarch 14, 2006AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-NLS-DNMT3A-L-N-spC9-N-intein-hGH
Plasmid#112212PurposeTargeted DNA methylationDepositorAvailable SinceOct. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pOP2HA
Plasmid#112598PurposeUsed for expression of protein in E. coli as non-cleavable C-terminal 8His-Avi tag fusionsDepositorTypeEmpty backboneTags8His-Avi tagExpressionBacterialAvailable SinceAug. 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTFE3-donor
Plasmid#112377PurposeCRISPR donor plasmid to tag human transcription factor TFE3 with GFPDepositorInsertTFE3 homology arms flanking EGFP-IRES-Neo cassette (TFE3 Human)
UseCRISPRMutationhg38:X:49030928:T>A,hg38:X:49030929:A>T,hg3…Available SinceDec. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLJM1-CRY2-mCherry-(GGGGS)3-hRGS2box
Plasmid#112257PurposeExpresses RGS component of opto-RGS2 in mammalian cellsDepositorInsertCRY2-mCherry-(GGGGS)3-hRGS2box
UseLentiviralTagsmCherryExpressionMammalianPromoterCMVAvailable SinceNov. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
CvcE in pGEX4t
Plasmid#112977PurposeFor protein expression and purification of Drosophila CvcEDepositorInsertcycE (CycE Fly)
ExpressionBacterialAvailable SinceAug. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
ACTB-(A)3x
Plasmid#112057PurposeACTB mRNA tagged with 3 copies of Riboglow (variant A)DepositorInsertACTB (ACTB Human)
TagsRiboglow RNA tagExpressionMammalianMutation3 copies of Riboglow tag (variant A) after stop c…PromoterCMVAvailable SinceJune 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
ACTB-(A)2x
Plasmid#112056PurposeACTB mRNA tagged with 2 copies of Riboglow (variant A)DepositorInsertACTB (ACTB Human)
TagsRiboglow RNA tagExpressionMammalianMutation2 copies of Riboglow tag (variant A) after stop c…PromoterCMVAvailable SinceJune 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-3xflagGREB1b
Plasmid#112222Purposemammalian expression of flag tagged GREB1bDepositorAvailable SinceJuly 20, 2018AvailabilityAcademic Institutions and Nonprofits only