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We narrowed to 2,481 results for: D10

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Showing: 421 - 440 of 2481 results

  1. pEvolvR-mCherry-Slug-nCas9(D10A)-PolI5MΔ

    Plasmid
    #249078
    Purpose
    Expresses nucleus-localized Slug-nCas9 (D10A) fused to PolI5MΔ and mCherry using a CMV promoter. Includes a GFP cassette flanked by BsmbI cut sites to knock in and coexpress user-defined gRNAs.
    Depositor
    John Dueber, David Schaffer
    Publication
    Hurtado et al Nat Commun. 2025 Apr 19;16(1):3723. doi:
    Insert
    Slug-nCas9-PolI5MΔ
    Use
    CRISPR
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    deletion of PolI5M N-terminal flap endonuclease d…
    Promoter
    CMV
    Available since
    Jan. 7, 2026
    Availability
    Academic Institutions and Nonprofits only
    249078-plasmid-map-sequence-id-496311

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  3. pEvolvR-mCherry-NNG-Slug-nCas9(D10A)-PolI5MΔ

    Plasmid
    #249079
    Purpose
    Expresses nucleus-localized NNG-Slug-nCas9 (D10A) fused to PolI5MΔ and mCherry using a CMV promoter. Includes a GFP cassette flanked by BsmbI cut sites to knock in and coexpress user-defined gRNAs.
    Depositor
    John Dueber, David Schaffer
    Publication
    Hurtado et al Nat Commun. 2025 Apr 19;16(1):3723. doi:
    Insert
    NNG-Slug-nCas9-PolI5MΔ
    Use
    CRISPR
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    deletion of PolI5M N-terminal flap endonuclease d…
    Promoter
    CMV
    Available since
    Jan. 7, 2026
    Availability
    Academic Institutions and Nonprofits only
    249079-plasmid-map-sequence-id-496312

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  4. T1-araC-Pbad-D10A/H840A cas9 ABCD-pACYC-BB

    Plasmid
    #110547
    Purpose
    CRISPR interference; works in conjunction with an sgRNA expression vector to silence genes in the E. coli chromosome.
    Depositor
    Ichiro Matsumura
    Insert
    dCas9
    Expression
    Bacterial
    Mutation
    D10A, H840A
    Promoter
    pBAD
    Available since
    July 24, 2018
    Availability
    Academic Institutions and Nonprofits only
    110547-plasmid-map-sequence-id-210556

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  5. pEF6_DmrB-[dltCARD]mCasp1(E102D103TRB_E121D122AA) (KS655)

    Plasmid
    #254936
    Purpose
    Murine Casp1 with its CARD replaced by DmrB, the 1st CDL cleavage site (E102D103) replaced by a Thrombin site, and the 2nd CDL cleavage site uncleavable (E121D122AA), in pEF6.
    Depositor
    Kate Schroder
    Publication
    Boucher et al J Exp Med. 2018 Mar 5;215(3):827-840. do
    Insert
    Casp1 (Casp1 Mouse)
    Tags
    DmrB
    Expression
    Bacterial and Mammalian
    Mutation
    E102D103TRB_E121D122AA
    Promoter
    EF-1a, T7
    Available since
    June 9, 2026
    Availability
    Academic Institutions and Nonprofits only
    254936-plasmid-map-sequence-id-512354

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  6. pHACK(Gal4)-DONR(T2A-D10A)

    Plasmid
    #250798
    Purpose
    Drosophila transgenic vector: HACK donor vector for inserting T2A-D10A into Gal4 coding sequence by genetic crosses, converting Gal4 into a T2A-D10A transgene.
    Depositor
    Chun Han
    Publication
    Shen et al bioRxiv 2025.10.30.685647
    Insert
    T2A-D10A
    Use
    CRISPR
    Expression
    Insect
    Available since
    May 28, 2026
    Availability
    Academic Institutions and Nonprofits only
    250798-plasmid-map-sequence-id-511575

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  7. pEvolvR-mCherry-enCas9(D10A)-PolI5MΔ

    Plasmid
    #249068
    Purpose
    Expresses nucleus-localized enCas9 (D10A) fused to PolI5MΔ and mCherry using a CMV promoter. Includes a GFP cassette flanked by BsmbI cut sites to knock in and coexpress user-defined gRNAs.
    Depositor
    John Dueber, David Schaffer
    Publication
    Hurtado et al Nat Commun. 2025 Apr 19;16(1):3723. doi:
    Insert
    enCas9-PolI5MΔ
    Use
    CRISPR
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    deletion of PolI5M N-terminal flap endonuclease d…
    Promoter
    CMV
    Available since
    Jan. 7, 2026
    Availability
    Academic Institutions and Nonprofits only
    249068-plasmid-map-sequence-id-496320

    Enlarge

  8. pEvolvR-mCherry-enCas9(D10A)-PolI5M

    Plasmid
    #249060
    Purpose
    Expresses nucleus-localized enCas9 (D10A) fused to PolI5M and mCherry using a CMV promoter. Includes a GFP cassette flanked by BsmbI cut sites to knock in and coexpress user-defined gRNAs.
    Depositor
    John Dueber, David Schaffer
    Publication
    Hurtado et al Nat Commun. 2025 Apr 19;16(1):3723. doi:
    Insert
    enCas9-PolI5M
    Use
    CRISPR
    Expression
    Mammalian
    Available since
    Dec. 23, 2025
    Availability
    Academic Institutions and Nonprofits only
    249060-plasmid-map-sequence-id-495988

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  13. pEF6_DmrB[dltCARD]mCasp1(C284A)(E102D103TRB_E121D122AA) (KS659)

    Plasmid
    #254937
    Purpose
    Catalytic mutant (C284A) of murine Casp1 with its CARD replaced by DmrB, the 1st CDL cleavage site (E102D103) replaced by a Thrombin site, and the 2nd CDL site uncleavable (E121D122AA), in pEF6.
    Depositor
    Kate Schroder
    Publication
    Boucher et al J Exp Med. 2018 Mar 5;215(3):827-840. do
    Insert
    Casp1 (Casp1 Mouse)
    Tags
    DmrB
    Expression
    Bacterial and Mammalian
    Mutation
    E102D103TRB_E121D122AA, C284A
    Promoter
    EF-1a, T7
    Available since
    June 22, 2026
    Availability
    Academic Institutions and Nonprofits only
    254937-plasmid-map-sequence-id-513614

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  15. pEvolvR-mCherry-Sniper-nCas9(D10A)-PolI5MΔ

    Plasmid
    #249071
    Purpose
    Expresses nucleus-localized Sniper-nCas9 (D10A) fused to PolI5MΔ and mCherry using a CMV promoter. Includes a GFP cassette flanked by BsmbI cut sites to knock in and coexpress user-defined gRNAs.
    Depositor
    John Dueber, David Schaffer
    Publication
    Hurtado et al Nat Commun. 2025 Apr 19;16(1):3723. doi:
    Insert
    Sniper-nCas9-PolI5MΔ
    Use
    CRISPR
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    deletion of PolI5M N-terminal flap endonuclease d…
    Promoter
    CMV
    Available since
    Jan. 7, 2026
    Availability
    Academic Institutions and Nonprofits only
    249071-plasmid-map-sequence-id-496323

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  16. pEvolvR-mCherry-SuperFi-nCas9(D10A)-PolI5MΔ

    Plasmid
    #249070
    Purpose
    Expresses nucleus-localized SuperFi-nCas9 (D10A) fused to PolI5MΔ and mCherry using a CMV promoter. Includes a GFP cassette flanked by BsmbI cut sites to knock in and coexpress user-defined gRNAs.
    Depositor
    John Dueber, David Schaffer
    Publication
    Hurtado et al Nat Commun. 2025 Apr 19;16(1):3723. doi:
    Insert
    SuperFi-nCas9-PolI5M?
    Use
    CRISPR
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    deletion of PolI5M N-terminal flap endonuclease d…
    Promoter
    CMV
    Available since
    Jan. 7, 2026
    Availability
    Academic Institutions and Nonprofits only
    249070-plasmid-map-sequence-id-496322

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  17. pEvolvR-mCherry-HSC1.2-nCas9(D10A)-PolI5MΔ

    Plasmid
    #249073
    Purpose
    Expresses nucleus-localized HSC1.2-nCas9 (D10A) fused to PolI5MΔ and mCherry using a CMV promoter. Includes a GFP cassette flanked by BsmbI cut sites to knock in and coexpress user-defined gRNAs.
    Depositor
    John Dueber, David Schaffer
    Publication
    Hurtado et al Nat Commun. 2025 Apr 19;16(1):3723. doi:
    Insert
    HSC1.2-nCas9-PolI5MΔ
    Use
    CRISPR
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    deletion of PolI5M N-terminal flap endonuclease d…
    Promoter
    CMV
    Available since
    Jan. 7, 2026
    Availability
    Academic Institutions and Nonprofits only
    249073-plasmid-map-sequence-id-496319

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  18. pEvolvR-mCherry-LZ3-nCas9(D10A)-PolI5MΔ

    Plasmid
    #249072
    Purpose
    Expresses nucleus-localized LZ3-nCas9 (D10A) fused to PolI5MΔ and mCherry using a CMV promoter. Includes a GFP cassette flanked by BsmbI cut sites to knock in and coexpress user-defined gRNAs.
    Depositor
    John Dueber, David Schaffer
    Publication
    Hurtado et al Nat Commun. 2025 Apr 19;16(1):3723. doi:
    Insert
    LZ3-nCas9-PolI5MΔ
    Use
    CRISPR
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    deletion of PolI5M N-terminal flap endonuclease d…
    Promoter
    CMV
    Available since
    Jan. 7, 2026
    Availability
    Academic Institutions and Nonprofits only
    249072-plasmid-map-sequence-id-496318

    Enlarge

  19. pEvolvR-mCherry-HF1-nCas9(D10A)-PolI5MΔ

    Plasmid
    #249075
    Purpose
    Expresses nucleus-localized HF1-nCas9 (D10A) fused to PolI5MΔ and mCherry using a CMV promoter. Includes a GFP cassette flanked by BsmbI cut sites to knock in and coexpress user-defined gRNAs.
    Depositor
    John Dueber, David Schaffer
    Publication
    Hurtado et al Nat Commun. 2025 Apr 19;16(1):3723. doi:
    Insert
    HF1-nCas9-PolI5MΔ
    Use
    CRISPR
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    deletion of PolI5M N-terminal flap endonuclease d…
    Promoter
    CMV
    Available since
    Jan. 7, 2026
    Availability
    Academic Institutions and Nonprofits only
    249075-plasmid-map-sequence-id-496315

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  20. pEvolvR-mCherry-Hypa-nCas9(D10A)-PolI5MΔ

    Plasmid
    #249076
    Purpose
    Expresses nucleus-localized Hypa-nCas9 (D10A) fused to PolI5MΔ and mCherry using a CMV promoter. Includes a GFP cassette flanked by BsmbI cut sites to knock in and coexpress user-defined gRNAs.
    Depositor
    John Dueber, David Schaffer
    Publication
    Hurtado et al Nat Commun. 2025 Apr 19;16(1):3723. doi:
    Insert
    Hypa-nCas9-PolI5MΔ
    Use
    CRISPR
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    deletion of PolI5M N-terminal flap endonuclease d…
    Promoter
    CMV
    Available since
    Jan. 7, 2026
    Availability
    Academic Institutions and Nonprofits only
    249076-plasmid-map-sequence-id-496314

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Showing: 421 - 440 of 2481 results