We narrowed to 469 results for: adk

1. pcDNA3.1 6A NLRP1 UPA-CARD-FLAG Y1445A

   Plasmid

   #163987

   Purpose

   Mammalian expression vector encoding human NLRP1 UPA-CARD (dimer interface mutant) with a C-terminal FLAG tag

   Depositor

   Hao Wu

   Article

   Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

   Insert

   NLRP1 UPA-CARD (NLRP1 Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Y1445A

   Available Since

   Feb. 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pcDNA3.1 6A NLRP1 UPA-CARD-FLAG R1427E

   Plasmid

   #163986

   Purpose

   Mammalian expression vector encoding human NLRP1 UPA-CARD (filament-deficient mutant) with a C-terminal FLAG tag

   Depositor

   Hao Wu

   Article

   Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

   Insert

   NLRP1 UPA-CARD (NLRP1 Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   R1427E

   Available Since

   Feb. 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pcDNA3.1 6A NLRP1 UPA-CARD-FLAG E1414R

   Plasmid

   #163985

   Purpose

   Mammalian expression vector encoding human NLRP1 UPA-CARD (filament-deficient mutant) with a C-terminal FLAG tag

   Depositor

   Hao Wu

   Article

   Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

   Insert

   NLRP1 UPA-CARD (NLRP1 Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   E1414R

   Available Since

   Feb. 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pcDNA3.1 6A NLRP1 UPA-CARD-FLAG E1411R

   Plasmid

   #163984

   Purpose

   Mammalian expression vector encoding human NLRP1 UPA-CARD (filament-deficient mutant) with a C-terminal FLAG tag

   Depositor

   Hao Wu

   Article

   Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

   Insert

   NLRP1 UPA-CARD (NLRP1 Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   E1411R

   Available Since

   Feb. 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pcDNA3.1 6A NLRP1 UPA-CARD-FLAG D1401R

   Plasmid

   #163983

   Purpose

   Mammalian expression vector encoding human NLRP1 UPA-CARD (filament-deficient mutant) with a C-terminal FLAG tag

   Depositor

   Hao Wu

   Article

   Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

   Insert

   NLRP1 UPA-CARD (NLRP1 Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   R1401R

   Available Since

   Feb. 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pcDNA3.1 6A NLRP1 UPA-CARD-FLAG E1397R

   Plasmid

   #163982

   Purpose

   Mammalian expression vector encoding human NLRP1 UPA-CARD (filament-deficient mutant) with a C-terminal FLAG tag

   Depositor

   Hao Wu

   Article

   Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

   Insert

   NLRP1 UPA-CARD (NLRP1 Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   E1397R

   Available Since

   Jan. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pcDNA3.1 6A NLRP1 UPA-CARD-FLAG R1392E

   Plasmid

   #163981

   Purpose

   Mammalian expression vector encoding human NLRP1 UPA-CARD (filament-deficient mutant) with a C-terminal FLAG tag

   Depositor

   Hao Wu

   Article

   Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

   Insert

   NLRP1 UPA-CARD (NLRP1 Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   R1392E

   Available Since

   Jan. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pcDNA3.1 6A NLRP1 UPA-CARD-FLAG R1386E

   Plasmid

   #163980

   Purpose

   Mammalian expression vector encoding human NLRP1 UPA-CARD (filament-deficient mutant) with a C-terminal FLAG tag

   Depositor

   Hao Wu

   Article

   Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

   Insert

   NLRP1 UPA-CARD (NLRP1 Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   R1386E

   Available Since

   Jan. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. TDP-REGv2(mScarlet-FLAG) matched positive control in pTwist-CMV

   Plasmid

   #216156

   Purpose

   Expresses mScarlet regardless of TDP-43 knockdown. Note: It is not recommended to produce lentiviruses containing TDP-REG sequences – see Depositor Comments

   Depositor

   Pietro Fratta

   Article

   Wilkins et al Science. 2024 Oct 4;386(6717):61-69. doi

   Insert

   mScarlet with C-terminal FLAG tag
   

   Expression

   Mammalian

   Available Since

   July 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pNon-target

    Plasmid

    #214053

    Purpose

    Expresses non-target sequence for Haliangium type III CRISPR-Cas complex; identical to pTarget (Addgene plasmid # 214052) but does not contain a protospacer.

    Depositor

    Raymond Staals

    Article

    Steens et al Science. 2024 Feb 2;383(6682):512-519. d

    Insert

    Non-target sequence
    

    Use

    Non-target rna plasmid

    Expression

    Bacterial

    Promoter

    trc promoter

    Available Since

    June 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pEcNucC

    Plasmid

    #214050

    Purpose

    Bacterial expression plasmid for E. coli MS 115-1 NucC, a cA3-responsive nuclease known to cause abortive infection; used as a positive control in the Haliangium ochraceum type III CRISPR-Cas system

    Depositor

    Raymond Staals

    Article

    Steens et al Science. 2024 Feb 2;383(6682):512-519. d

    Insert

    EcNucC
    

    Expression

    Bacterial

    Mutation

    WT

    Promoter

    lacUV5 promoter

    Available Since

    June 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pcDNA3.1 NLRP1-delPYD-delCARD-TEV-GFP-FLAG codon-optimized

    Plasmid

    #166834

    Purpose

    Expression and purification of NLRP1 (without the PYD and CARD) from mammalian cells

    Depositor

    Hao Wu

    Article

    Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

    Insert

    NLRP1 (NLRP1 Human)
    

    Tags

    TEV-GFP-FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pSAVED-CHAT-cbm_PCaspase

    Plasmid

    #214047

    Purpose

    Bacterial expression plasmid for SAVED-CHAT cA3 binding pocket mutant and PCaspase from Haliangium ochraceum

    Depositor

    Raymond Staals

    Article

    Steens et al Science. 2024 Feb 2;383(6682):512-519. d

    Insert

    SAVED-CHAT-PCaspase
    

    Expression

    Bacterial

    Mutation

    K121E, Q122A, N274A, R276E, Y285A, F286A

    Promoter

    lacUV5 promoter

    Available Since

    Aug. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pSAVED-CHAT_PCaspase-N-C-term

    Plasmid

    #214046

    Purpose

    Bacterial expression plasmid for SAVED-CHAT, PCaspase N-terminal fragment (aa 1-153), and C-terminal fragment (154-666) from Haliangium ochraceum

    Depositor

    Raymond Staals

    Article

    Steens et al Science. 2024 Feb 2;383(6682):512-519. d

    Insert

    SAVED-CHAT-PCaspase
    

    Expression

    Bacterial

    Mutation

    aa 1-153, 154-666

    Promoter

    lacUV5 promoter

    Available Since

    June 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pSAVED-CHAT-sm_PCaspase

    Plasmid

    #214048

    Purpose

    Bacterial expression plasmid for SAVED-CHAT CHAT-CHAT singlet interface mutant and PCaspase from Haliangium ochraceum

    Depositor

    Raymond Staals

    Article

    Steens et al Science. 2024 Feb 2;383(6682):512-519. d

    Insert

    SAVED-CHAT-PCaspase
    

    Expression

    Bacterial

    Mutation

    R456E, D459K, D476K, R479E

    Promoter

    lacUV5 promoter

    Available Since

    June 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pJS-BCD-Strep-SAVED-CHAT

    Plasmid

    #214030

    Purpose

    Bacterial expression plasmid for strep-tagged wild-type SAVED-CHAT from Haliangium ochraceum

    Depositor

    Raymond Staals

    Article

    Steens et al Science. 2024 Feb 2;383(6682):512-519. d

    Insert

    SAVED-CHAT
    

    Tags

    Strep-tag II

    Expression

    Bacterial

    Mutation

    WT

    Promoter

    T7 promoter

    Available Since

    June 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pJS-BCD-Strep-dSAVED-CHAT

    Plasmid

    #214031

    Purpose

    Bacterial expression plasmid for strep-tagged catalytically dead SAVED-CHAT mutant from Haliangium ochraceum

    Depositor

    Raymond Staals

    Article

    Steens et al Science. 2024 Feb 2;383(6682):512-519. d

    Insert

    SAVED-CHAT
    

    Tags

    Strep-tag II

    Expression

    Bacterial

    Mutation

    H375A, C422A

    Promoter

    T7 promoter

    Available Since

    June 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pJS-BCD-Strep-SAVED-CHAT-sm

    Plasmid

    #214033

    Purpose

    Bacterial expression plasmid for strep-tagged SAVED-CHAT CHAT-CHAT singlet interface mutant from Haliangium ochraceum

    Depositor

    Raymond Staals

    Article

    Steens et al Science. 2024 Feb 2;383(6682):512-519. d

    Insert

    SAVED-CHAT
    

    Tags

    Strep-tag II

    Expression

    Bacterial

    Mutation

    R456E, D459K, D476K, R479E

    Promoter

    T7 promoter

    Available Since

    June 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pJS-BCD-Strep-PCaspase

    Plasmid

    #214034

    Purpose

    Bacterial expression plasmid for strep-tagged PCaspase (Prokaryotic Caspase) from Haliangium ochraceum

    Depositor

    Raymond Staals

    Article

    Steens et al Science. 2024 Feb 2;383(6682):512-519. d

    Insert

    PCaspase
    

    Tags

    Strep-tag II

    Expression

    Bacterial

    Mutation

    WT

    Promoter

    T7 promoter

    Available Since

    June 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA 3.1 LIC 6A NLRP1 UPA-CARD-FLAG E1322R

    Plasmid

    #166814

    Purpose

    Mammalian cell expression vector for FLAG-tagged UPA-CARD of NLRP1. The E1322R mutant abolishes interface III UPA-UPA contacts, DPP9 binding, and inflammasome activity

    Depositor

    Hao Wu

    Article

    Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

    Insert

    NLRP1 (NLRP1 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    E1322R

    Promoter

    CMV

    Available Since

    May 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge