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pE-SUMO CRYBA2

Plasmid

#80746

Purpose

Expression of human beta A2-crystallin in E. coli

Depositor

Larry David

Publication

Halverson-Kolkind et al Exp Eye Res. 2024 Nov;248:110115. doi: 1

Insert

CRYBA2 (CRYBA2 Human)

Tags

6xHIS and Smt3 SUMO

Expression

Bacterial

Promoter

T7

Available since

March 9, 2017

Availability

Academic Institutions and Nonprofits only

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pE-SUMO CRYGB

Plasmid

#80751

Purpose

Expression of human gamma B-crystallin in E. coli

Depositor

Larry David

Publication

Halverson-Kolkind et al Exp Eye Res. 2024 Nov;248:110115. doi: 1

Insert

CRYGB (CRYGB Human)

Tags

6xHIS and Smt3 SUMO

Expression

Bacterial

Promoter

T7

Available since

March 9, 2017

Availability

Academic Institutions and Nonprofits only

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PColDuet-nHis-SsDddI.dna

Plasmid

#254901

Purpose

Expresses of Simiaoa sunii DddI(SsDddI) in BL21(DE3) cells

Depositor

Andrew Stergachis

Publication

Swanson et al Nat Biotechnol. 2025 Dec 3. doi: 10.1038

Insert

SsDddI

Tags

6xHis

Expression

Bacterial

Promoter

T7 promoter

Available since

June 1, 2026

Availability

Academic Institutions and Nonprofits only

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pE-SUMO CRYGC

Plasmid

#80754

Purpose

Expression of human gamma C-crystallin in E. coli

Depositor

Larry David

Publication

Halverson-Kolkind et al Exp Eye Res. 2024 Nov;248:110115. doi: 1

Insert

CRYGC (CRYGC Human)

Tags

6xHIS and Smt3 SUMO

Expression

Bacterial

Promoter

T7

Available since

March 9, 2017

Availability

Academic Institutions and Nonprofits only

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pESUMO-hRORg2

Plasmid

#170329

Purpose

Expresses RORg isoform 2 with N-terminal HisSUMO tag in bacteria

Depositor

Patrick Griffin

Publication

Strutzenberg et al J Mol Biol. 2021 Sep 20;433(22):167258.

Insert

RORG isoform 2 (RORC Human)

Tags

HisSUMO

Expression

Bacterial

Mutation

codon optimized

Promoter

T7

Available since

Sept. 20, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pESUMO-hRORg1

Plasmid

#170330

Purpose

Expresses RORg isoform 1 with N-terminal HisSUMO tag in bacteria

Depositor

Patrick Griffin

Publication

Strutzenberg et al J Mol Biol. 2021 Sep 20;433(22):167258.

Insert

RORG isoform 1 (RORC Human)

Tags

HisSUMO

Expression

Bacterial

Promoter

T7

Available since

Sept. 20, 2021

Availability

Academic Institutions and Nonprofits only

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p416TEF-NQYFP

Plasmid

#99552

Purpose

Expresses Swi1 NQ-YFP to monitor Swi1 state or induce [SWI+].

Depositor

Liming Li

Publication

Du et al Mol Cell Biol. 2010 Oct;30(19):4644-55.

Insert

Swi1 NQ-rich region (AA1-536) (SWI1 Budding Yeast)

Tags

YFP

Expression

Yeast

Promoter

TEF1

Available since

Aug. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_Hygro(+)_GRHL1-K370A-L3F6H

Plasmid

#177771

Purpose

Overexpression of 3xFLAG-6xHis tagged human GRHL1 (K370A mutation)

Depositor

Michael Ristow

Publication

Grigolon et al Nat Commun. 2022 Jan 10;13(1):107. doi:

Insert

GRHL1 (GRHL1 Human)

Expression

Mammalian

Mutation

K370A

Available since

April 5, 2022

Availability

Academic Institutions and Nonprofits only

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pSM2

Plasmid

#85753

Purpose

pDONR split marker vector (GenBank accession number: KX904529) for fungal gene deletion. Used with pSM1.

Depositor

Damien Fleetwood

Publication

Rahnama et al J Microbiol Methods. 2016 Dec 22. pii: S

Type

Empty backbone

Expression

Bacterial

Promoter

N/A

Available since

Aug. 4, 2017

Availability

Academic Institutions and Nonprofits only

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pSM1

Plasmid

#85752

Purpose

pDONR split marker vector (GenBank accession number: KX904528) for fungal gene deletion. Used with pSM2.

Depositor

Damien Fleetwood

Publication

Rahnama et al J Microbiol Methods. 2016 Dec 22. pii: S

Type

Empty backbone

Expression

Bacterial

Promoter

N/A

Available since

Aug. 4, 2017

Availability

Academic Institutions and Nonprofits only

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PKT2/CLP-Vector

Plasmid

#239611

Purpose

Transposon vector

Depositor

Xin-yuan Guan

Publication

Gong et al Cell. 2025 Jul 24:S0092-8674(25)00793-7.

Type

Empty backbone

Use

Transposon

Expression

Mammalian

Available since

Aug. 15, 2025

Availability

Academic Institutions and Nonprofits only

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shortVGLL3-GFP

Plasmid

#241148

Purpose

expresses short isoform of VGLL3 tagged by GFP

Depositor

Itamar Harel

Publication

Moses et al Nat Commun. 2026 Jun 2;17(1):4410. doi:

Insert

shortVGLL3-GFP (vgll3 N. furzeri)

Tags

GFP

Expression

Bacterial

Promoter

ef1a

Available since

July 22, 2026

Availability

Academic Institutions and Nonprofits only

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longVGLL3-GFP

Plasmid

#241149

Purpose

expresses long isoform of VGLL3 tagged by GFP

Depositor

Itamar Harel

Publication

Moses et al Nat Commun. 2026 Jun 2;17(1):4410. doi:

Insert

longVGLL3-GFP (vgll3 N. furzeri)

Tags

GFP

Expression

Bacterial

Promoter

ef1a

Available since

July 22, 2026

Availability

Academic Institutions and Nonprofits only

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pGL4.23-WIPI1promotor

Plasmid

#206840

Purpose

The plasmid contains the human WIPI1 promotor sequence (-1226 bp upstream to +329 bp downstream of the transcription start site) cloned into pGL4.23[luc2/minP] (NheI/HindIII).

Depositor

Tassula Proikas-Cezanne

Publication

Sporbeck et al Commun Biol. 2023 Aug 24;6(1):872. doi:

Insert

WIPI1 promotor (partial)

Use

Luciferase

Available since

Jan. 21, 2025

Availability

Academic Institutions and Nonprofits only

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pColADuet-DddA-DddI-WT

Plasmid

#254736

Purpose

Expresses Simiaoa sunii DddA(SsDddA) and DddI(SsDddI) in BL21(DE3) cells

Depositor

Andrew Stergachis

Publication

Swanson et al Nat Biotechnol. 2025 Dec 3. doi: 10.1038

Inserts

DddA
DddI

Tags

N-6His tag

Expression

Bacterial

Available since

Aug. 3, 2026

Availability

Academic Institutions and Nonprofits only

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pdestMB14_grh-1p-grh-1-gfp

Plasmid

#177761

Purpose

Overexpression of C. elegans grh-1

Depositor

Michael Ristow

Publication

Grigolon et al Nat Commun. 2022 Jan 10;13(1):107. doi:

Insert

grh-1 (grh-1 Nematode)

Expression

Worm

Available since

April 5, 2022

Availability

Academic Institutions and Nonprofits only

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PX459_GRHL1-exon5

Plasmid

#177763

Purpose

sgRNA for CRISPR/Cas9-mediated deletion of human GRHL1

Depositor

Michael Ristow

Publication

Grigolon et al Nat Commun. 2022 Jan 10;13(1):107. doi:

Insert

GRHL1 (GRHL1 Human)

Use

CRISPR

Available since

April 5, 2022

Availability

Academic Institutions and Nonprofits only

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PX459_GRHL1-exon8

Plasmid

#177764

Purpose

sgRNA for CRISPR/Cas9-mediated deletion of human GRHL1

Depositor

Michael Ristow

Publication

Grigolon et al Nat Commun. 2022 Jan 10;13(1):107. doi:

Insert

GRHL1 (GRHL1 Human)

Use

CRISPR

Available since

April 5, 2022

Availability

Academic Institutions and Nonprofits only

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pPN095

Plasmid

#91622

Purpose

Express sgRNA targeting human GRIN2A

Depositor

Lindy Barrett

Publication

Hazelbaker et al Stem Cell Reports. 2017 Oct 10;9(4):1315

Insert

GRIN2A (GRIN2A Human)

Use

CRISPR

Promoter

U6

Available since

Oct. 12, 2017

Availability

Academic Institutions and Nonprofits only

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PKT2/CLP-Aoah

Plasmid

#239612

Purpose

Expresses mouse Aoah protein in the transposon vector

Depositor

Xin-yuan Guan

Publication

Gong et al Cell. 2025 Jul 24:S0092-8674(25)00793-7.

Insert

Acyloxyacyl Hydrolase (Aoah Mouse)

Use

Transposon

Tags

HA

Expression

Mammalian

Available since

Aug. 15, 2025

Availability

Academic Institutions and Nonprofits only

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