We narrowed to 711 results for: pgl3

1. pGL3-24p3/LCN2-282C/EBP mut-LUC

   Plasmid

   #46868

   Purpose

   murine 24p3 minimal promoter with C/EBP mutation k as a luciferase reporter

   Depositor

   Sarah Gaffen

   Article

   Maitra et al Proc Natl Acad Sci U S A. 2007 May 1;104

   Insert

   24p3/lipocalin 2 proximal promoter
   

   Use

   Luciferase

   Tags

   Expression

   Mutation

   CEBP site is mutated (see comments)

   Promoter

   24p3 promoter (inactive)

   Available since

   Sept. 13, 2013

   Availability

   Academic Institutions and Nonprofits only

2. pGL3-MTS-CCDB-N136-RsDddA-B-UGI-PURO

   Plasmid

   #205464

   Purpose

   A backbone containing a RsDddA fragment B for constructing mitochondria-targeted RsDdCBE expression plasmid

   Depositor

   Bin Shen

   Article

   Cheng et al Mol Ther Nucleic Acids. 2023 Sep 9;34:10

   Insert

   MTS; N136; ccdb; RsDddA-B; UGI
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pGL3-MTS-CCDB-N136-RsDddA-D-UGI-PURO

   Plasmid

   #205466

   Purpose

   A backbone containing a RsDddA fragment D for constructing mitochondria-targeted RsDdCBE expression plasmid

   Depositor

   Bin Shen

   Article

   Cheng et al Mol Ther Nucleic Acids. 2023 Sep 9;34:10

   Insert

   MTS; N136; ccdb; RsDddA-D; UGI
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pGL3-luciferase-reporter-CHAF1B-SVA-del-VNTR

   Plasmid

   #202830

   Purpose

   pGL3 luciferase reporter with CHAF1B-SVA-del-VNTR sequence cloned upstream of SV40 promoter to test effects of SVA-del-VNTR insertion on luciferase expression

   Depositor

   Matthew Anderson

   Article

   Nadler et al Commun Biol. 2023 Mar 30;6(1):347. doi:

   Insert

   CHAF1B intronic SVA del-VNTR sequence
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   SV40

   Available since

   July 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pGL3-MTS-COX2-Q6-R18-G1333C-UGI-Puro

   Plasmid

   #198845

   Purpose

   Employ DdCBE-mediated mtDNA editing to ablate mtProtein(COX2) by swapping Gln codon CAA with stop codon TAA in C6 cells

   Depositor

   Bin Shen

   Article

   Tan et al Sci Adv. 2023 Apr 14;9(15):eadf2695. doi

   Insert

   COX2-Q6-R18 TALEN
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pGL3-MTS-ATP8-W9-R15-G1333N-UGI-Puro

   Plasmid

   #198841

   Purpose

   Employ DdCBE-mediated mtDNA editing to ablate mtProtein(ATP8) by swapping Trp codon TGA with stop codon TAA in C6 cells

   Depositor

   Bin Shen

   Article

   Tan et al Sci Adv. 2023 Apr 14;9(15):eadf2695. doi

   Insert

   ATP8-W9-R15 TALEN
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pGL3-MTS-ATP8-W9-L17-G1333C-UGI-Puro

   Plasmid

   #198840

   Purpose

   Employ DdCBE-mediated mtDNA editing to ablate mtProtein(ATP8) by swapping Trp codon TGA with stop codon TAA in C6 cells

   Depositor

   Bin Shen

   Article

   Tan et al Sci Adv. 2023 Apr 14;9(15):eadf2695. doi

   Insert

   ATP8-W9-L17 TALEN
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pGL3-MTS-COX2-Q6-L18-G1333N-UGI-Puro

   Plasmid

   #198844

   Purpose

   Employ DdCBE-mediated mtDNA editing to ablate mtProtein(COX2) by swapping Gln codon CAA with stop codon TAA in C6 cells

   Depositor

   Bin Shen

   Article

   Tan et al Sci Adv. 2023 Apr 14;9(15):eadf2695. doi

   Insert

   COX2-Q6-L18 TALEN
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pGL3-NLS-ccdb-DddA(G1333C)-UGI-Puro

   Plasmid

   #173232

   Purpose

   One-step assembly of custom RVD arrays to construct DdCBE, TALE, etc.

   Depositor

   Bin Shen

   Article

   Guo et al Cell Discov. 2021 Sep 3;7(1):78. doi: 10

   Type

   Empty backbone

   Use

   Unspecified

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pGL3-NLS-ccdb-DddA(G1333N)-UGI-Puro

    Plasmid

    #173233

    Purpose

    One-step assembly of custom RVD arrays to construct DdCBE, TALE, etc.

    Depositor

    Bin Shen

    Article

    Guo et al Cell Discov. 2021 Sep 3;7(1):78. doi: 10

    Type

    Empty backbone

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pGL3-NLS-ccdb-DddA(G1397C)-UGI-Puro

    Plasmid

    #173234

    Purpose

    One-step assembly of custom RVD arrays to construct DdCBE, TALE, etc.

    Depositor

    Bin Shen

    Article

    Guo et al Cell Discov. 2021 Sep 3;7(1):78. doi: 10

    Type

    Empty backbone

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pGL3-NLS-ccdb-DddA(G1397N)-UGI-Puro

    Plasmid

    #173235

    Purpose

    One-step assembly of custom RVD arrays to construct DdCBE, TALE, etc.

    Depositor

    Bin Shen

    Article

    Guo et al Cell Discov. 2021 Sep 3;7(1):78. doi: 10

    Type

    Empty backbone

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pGL3- sgAAVS1.5-CAG-Cas9-T2A-mCherry-P2A-Puro

    Plasmid

    #216246

    Purpose

    Express Cas9 with CAG promoter to improve the expression in hES cells and a highly efficient sgAAVS1.5 for AAVS1 integration

    Depositor

    Elina Ikonen

    Article

    Li et al Genome Biol. 2024 Feb 26;25(1):58. doi:

    Insert

    Cas9 and sgAAVS1.5
    

    Use

    CRISPR; Human safe harbor locus (a avs1) site-spe…

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    March 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pGL3-sgSEC61B.N1-CAG-Cas9-T2A-mCherry-P2A-Puro

    Plasmid

    #216251

    Purpose

    Express Cas9 with CAG promoter to improve the expression in hES cells and sgSEC61B.N1 to tag endogenous SEC61B N-terminus

    Depositor

    Elina Ikonen

    Article

    Li et al Genome Biol. 2024 Feb 26;25(1):58. doi:

    Insert

    Cas9 and sgSEC61B.N1 (SEC61B Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    March 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pGL3-MTS-G3635A-L17-Q2L7-T2176N-UGI-Puro

    Plasmid

    #209647

    Purpose

    To install m.G3635 mutation on mtDNA

    Depositor

    Bin Shen

    Article

    Sun et al Nat Commun. 2023 Oct 19;14(1):6625. doi:

    Insert

    L17-Q2L7-T2176N
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pGL3-MTS-G3635A-L17-Q2L7-T2176C-UGI-Puro

    Plasmid

    #209646

    Purpose

    To install m.G3635 mutation on mtDNA

    Depositor

    Bin Shen

    Article

    Sun et al Nat Commun. 2023 Oct 19;14(1):6625. doi:

    Insert

    L17-Q2L7-T2176C
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pGL3-MTS-G3635A-L17-FZY2-S100N-UGI-Puro

    Plasmid

    #209642

    Purpose

    To install m.G3635 mutation on mtDNA

    Depositor

    Bin Shen

    Article

    Sun et al Nat Commun. 2023 Oct 19;14(1):6625. doi:

    Insert

    L17-FZY2-S100N
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pGL3-MTS-G3635A-L17-FZY2-S100C-UGI-Puro

    Plasmid

    #209641

    Purpose

    To install m.G3635 mutation on mtDNA

    Depositor

    Bin Shen

    Article

    Sun et al Nat Commun. 2023 Oct 19;14(1):6625. doi:

    Insert

    L17-FZY2-S100C
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pGL3-MTS-G3635A-R16-Q2L7-T2176N-UGI-Puro

    Plasmid

    #209649

    Purpose

    To install m.G3635 mutation on mtDNA

    Depositor

    Bin Shen

    Article

    Sun et al Nat Commun. 2023 Oct 19;14(1):6625. doi:

    Insert

    R16-Q2L7-T2176N
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pGL3-sgRANGAP.C1-CAG-Cas9-T2A-mCherry-P2A-Puro

    Plasmid

    #216249

    Purpose

    Express Cas9 with CAG promoter to improve the expression in hES cells and sgRANGAP.C1 to tag endogenous RANGAP1 C-terminus

    Depositor

    Elina Ikonen

    Article

    Li et al Genome Biol. 2024 Feb 26;25(1):58. doi:

    Insert

    Cas9 and sgRANGAP.C1 (RANGAP1 Human)
    

    Use

    CRISPR; Endogenous tagging

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    March 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge