We narrowed to 6,070 results for: nls

1. G1333 DddAtox-C–SaKKH-Cas9(D10A)

   Plasmid

   #157838

   Purpose

   expresses split DddAtox-Cas9 construct in mammalian cells

   Depositor

   David Liu

   Article

   Mok et al Nature. 2020 Jul 8. pii: 10.1038/s41586-

   Insert

   G1333 DddAtox-C–SaKKH-Cas9(D10A)–UGI–SV40 NLS
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. G1397 DddAtox-N–SaKKH-Cas9(D10A)

   Plasmid

   #157839

   Purpose

   expresses split DddAtox-Cas9 construct in mammalian cells

   Depositor

   David Liu

   Article

   Mok et al Nature. 2020 Jul 8. pii: 10.1038/s41586-

   Insert

   G1397 DddAtox-N–SaKKH-Cas9(D10A)–UGI–SV40 NLS
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. SpyCas9 PE2-3HA

   Plasmid

   #169853

   Purpose

   Mammalian Expression, SpyCas9 prime editor with 3HA-tag

   Depositor

   Scot Wolfe

   Article

   Liu et al Nat Commun. 2021 Apr 9;12(1):2121. doi:

   Insert

   SpCas9-H840A-M-MLV-3HA
   

   Use

   Tags

   3XHA tag and bpSV40 NLS

   Expression

   Mammalian

   Mutation

   Promoter

   cmv

   Available since

   June 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pX330-Flag-dHeFSpCas9

   Plasmid

   #92116

   Purpose

   Expression plasmid for human codon-optimized dead/inactive increased fidelity HeFSpCas9 (without U6-sgRNA coding sequence)

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:

   Insert

   dead/inactive HeFSpCas9 with FLAG tag
   

   Use

   CRISPR

   Tags

   3xFLAG and NLS

   Expression

   Mammalian

   Mutation

   D10A, N497A, R661A, Q695A, H840A, K848A, Q926A, K…

   Promoter

   Cbh

   Available since

   Oct. 11, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. nuc-yHS1-M7A

   Plasmid

   #159167

   Purpose

   Nuclear labile heme reporter

   Depositor

   Amit Reddi

   Article

   Hanna et al Proc Natl Acad Sci U S A. 2016 Jul 5;113

   Insert

   nuc-yHS1-M7A
   

   Use

   Tags

   SV40 NLS

   Expression

   Yeast

   Mutation

   Mutated Met 7 of the cyt b562 module of nuc-yHS1 …

   Promoter

   GPD

   Available since

   Sept. 21, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. pX330-Flag-HiFi SpCas9 (without sgRNA; with silent mutations)

   Plasmid

   #126778

   Purpose

   Expression plasmid for human codon-optimized increased fidelity HiFi SpCas9 (without U6-sgRNA coding sequence, with silent mutations)

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:

   Insert

   HiFi SpCas9
   

   Use

   CRISPR

   Tags

   3xFLAG and NLS

   Expression

   Mammalian

   Mutation

   R691A

   Promoter

   CBh

   Available since

   March 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pTE3987

   Plasmid

   #137716

   Purpose

   Expresses dLbCas12a-RVRR-BE in mammalian cells.

   Depositor

   Ervin Welker

   Article

   Toth et al Nucleic Acids Res. 2020 Feb 28. pii: 576

   Insert

   dLbCas12a-RVRR-BE
   

   Use

   Tags

   6xHis, APOBEC-1, SV40 NLS, and UGI

   Expression

   Mammalian

   Mutation

   G532A, K538V, Y542R, K595R

   Promoter

   CMV

   Available since

   March 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pmCherry-NES-OptoJNKi

   Plasmid

   #89739

   Purpose

   Expression of light-regulated JNK inhibitor, mCherry-tagged, localised to cytoplasm, wild-type photosensor, inhibitor JIP11

   Depositor

   Michael Courtney

   Article

   Melero-Fernandez de Mera et al Nat Commun. 2017 May 12;8:15017. doi: 10

   Insert

   NLS-OptoJNKi
   

   Use

   Fluorescent protein

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3-Ro2

   Plasmid

   #24503

   Depositor

   Bruce Conklin

   Article

   Coward et al Proc Natl Acad Sci U S A. 1998 Jan 6;95(

   Insert

   RASSL Ro2 (OPRK1 Human)
   

   Use

   Tags

   FLAG and NLS from prolactin

   Expression

   Mammalian

   Mutation

   mutations in extracellular loops 2 (see comments …

   Promoter

   Available since

   June 16, 2010

   Availability

   Academic Institutions and Nonprofits only

10. JPF0522

    Plasmid

    #124013

    Purpose

    Encodes crippled pEF1a driving expression of mAzamiGreen with an mScarlet normalization in a PiggyBac destination vector

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    PB_pEF1ac-NLS-mAzamiGreen-bghpA_pCAG-H2B::mScarlet-rglpA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pX330-Flag-Sniper SpCas9 (without sgRNA; with silent mutations)

    Plasmid

    #126777

    Purpose

    Expression plasmid for human codon-optimized increased fidelity Sniper SpCas9 (without U6-sgRNA coding sequence, with silent mutations)

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:

    Insert

    Sniper SpCas9
    

    Use

    CRISPR

    Tags

    3xFLAG and NLS

    Expression

    Mammalian

    Mutation

    F539S, M763I, K890N

    Promoter

    CBh

    Available since

    March 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pPB758

    Plasmid

    #210033

    Purpose

    Contains inducible Cas7-P2A-Cas5-T2A-Cas8, rtTA-T2A-puro

    Depositor

    Michael Talkowski

    Article

    Nuttle et al Cell Rep Methods. 2023 Dec 5:100672. doi

    Inserts

    Cas7-P2A-Cas5-T2A-Cas8
    rtta-T2A-puro
    

    Use

    CRISPR

    Tags

    SV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1α and TRE3G

    Available since

    Feb. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMSCV-FKBP-H3(pLxIS-PLPLR)

    Plasmid

    #221287

    Purpose

    Expression of H3 (pLxIS-PLPLR) in mammalian cells by retroviral transduction

    Depositor

    Jonathan Kagan

    Article

    Landau et al Mol Cell. 2024 Jul 11;84(13):2436-2454.e

    Insert

    H3 (pLxIS-PLPLR) (H3 Synthetic)
    

    Use

    Retroviral

    Tags

    3xFLAG-4xFKBP

    Expression

    Mutation

    K to Q mutations made to inhibit NLS

    Promoter

    Available since

    July 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMZ828

    Plasmid

    #155322

    Purpose

    Constitutive expression of PhyB(1-650)-VP16 in plants

    Depositor

    Matias Zurbriggen

    Article

    Ochoa-Fernandez et al Nat Methods. 2020 Jul;17(7):717-725. doi

    Insert

    PCaMV35S-PhyB(1-650)-VP16-NLS-Tnos
    

    Use

    Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Oct. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pYPQ230-D156R

    Plasmid

    #188542

    Purpose

    Gateway entry clone (attL1 & attR5) for LbCas12a-D156R mediated genome editing

    Depositor

    Yiping Qi

    Article

    Zhang et al Genome Biol. 2023 Apr 30;24(1):102. doi:

    Insert

    LbCas12a-D156R
    

    Use

    CRISPR; Gateway compatible lbcas12a-d156r entry c…

    Tags

    NLS

    Expression

    Plant

    Mutation

    D156R

    Promoter

    Available since

    May 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pWP172

    Plasmid

    #198796

    Purpose

    Co-expression plasmid for the OLL neurons

    Depositor

    Paul Sternberg

    Article

    Nava et al Proc Natl Acad Sci U S A. 2023 Dec 19;12

    Insert

    Pseb-3-NLS-gp41-1-C-intein-cGAL(AD)
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    May 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pWP170

    Plasmid

    #198792

    Purpose

    Co-expression plasmid for the FLP, PVD neurons

    Depositor

    Paul Sternberg

    Article

    Nava et al Proc Natl Acad Sci U S A. 2023 Dec 19;12

    Insert

    PY48G10A.6-NLS-gp41-1-C-intein-cGAL(AD)
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    April 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pWP095

    Plasmid

    #198789

    Purpose

    Co-expression plasmid for the CEP neurons

    Depositor

    Paul Sternberg

    Article

    Nava et al Proc Natl Acad Sci U S A. 2023 Dec 19;12

    Insert

    PF35D11.1-NLS-cGAL(DBD)-gp41-1-N-intein
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    April 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pWP171

    Plasmid

    #198795

    Purpose

    Co-expression plasmid for the OLL neurons

    Depositor

    Paul Sternberg

    Article

    Nava et al Proc Natl Acad Sci U S A. 2023 Dec 19;12

    Insert

    Peat4prom9-NLS-cGAL(DBD)-gp41-1-N-intein
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    April 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pWP063

    Plasmid

    #198799

    Purpose

    Co-expression plasmid for the PHB neurons

    Depositor

    Paul Sternberg

    Article

    Nava et al Proc Natl Acad Sci U S A. 2023 Dec 19;12

    Insert

    Posm-10-NLS-cGAL(DBD)-gp41-1-N-intein
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    April 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge