We narrowed to 13,228 results for: sequence
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Plasmid#68833PurposeExpression of Bmal1-luc in mammalian cellsDepositorInsertBmal1 regulatory sequences (Bmal1 Mouse)
UseLentiviral and LuciferaseTagsluciferaseExpressionMammalianAvailable SinceAug. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGFP FAK Y397F
Plasmid#50516PurposeThis plasmid contains GFP21, which has a sequence similar to YFP, but emission/excitation similar to GFP.DepositorInsertprotein tyrosine kinase, mutated
TagsGFPExpressionMammalianMutationY397FPromoterCMVAvailable SinceJan. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-RFP-SKL
Plasmid#183726PurposeFor expression of RFP targeted to peroxisomesDepositorInsertmRFP1
UseLentiviralTagsSKL (peroxisomal targeting sequence 1)PromoterUbiCAvailable SinceNov. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-EGFP-SKL
Plasmid#183727PurposeFor exrpression of GFP targeted to peroxisomesDepositorInsertEGFP
UseLentiviralTagsSKL (peroxisomal targeting sequence 1)PromoterUbiCAvailable SinceNov. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
OmpA3-PNGaseF-TEV-His6
Plasmid#114274PurposeAmidase removing almost all N-linked oligosaccharides from glycoproteins. This PNGase F construct can have its hexa-his removed.DepositorInsertPeptide: N-glycosidase F
TagsOmpA3 leader sequence (for periplasmic localisati…ExpressionBacterialPromoterT7Available SinceAug. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pOpen-ETSSB
Plasmid#165549PurposessDNA binding protein, 9kDa. Features: Improves the processivity of DNA polymerase; involved in stabilization and marking of ssDNA structure; increases the yield and specificitiy of PCR; increases the yield and processivity of RT during RT-PCR; improves DNA sequencing through regions with strong secondary structureDepositorInsertExtreme Thermostable Single-Stranded DNA Binding Protein
UseSynthetic BiologyAvailable SinceAug. 10, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pHAGE-mNeptune2-SKL
Plasmid#183729PurposeFor exrpression of mNeptune2 targeted to peroxisomesDepositorInsertmNeptube2
UseLentiviralTagsSKL (peroxisomal targeting sequence 1)PromoterUbiCAvailable SinceNov. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAd/WT1-IRES-nAmCyan
Plasmid#29756DepositorInsertWilms tumor 1 (WT1 Human)
TagsAmCyan, IRES, and Nuclear localization sequenceExpressionMammalianMutationNo mutations; AmCyan is nuclear localized; WT1 an…Available SinceOct. 5, 2011AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-mCardinal-SKL
Plasmid#183728PurposeFor exrpression of mCardinal targeted to peroxisomesDepositorInsertmCardinal
UseLentiviralTagsSKL (peroxisomal targeting sequence 1)PromoterUbiCAvailable SinceNov. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
O-TREM2-2
Plasmid#166545PurposescFv of a human scaffold targeting Triggering receptor expressed on myeloid cells 2. Antigen coverage aa 19-131 of 230DepositorInsertsingle chain-Fv, scFv
UseRecombinant antibody expressionTags3xFLAG, His6 and OmpA leader sequenceExpressionBacterialPromoterLacZAvailable SinceApril 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHR_SFFV*/Puro2a-GFP
Plasmid#198222PurposeLentiviral plasmid derived from pHR_SFFV (Addgene #79121) expressing cDNA for a codon-optimized puromycin resistance gene, followed by an FMDV 2A domain and then EGFPDepositorInsertEGFP
UseLentiviralTagsCodon-optimized puromycin resistance gene, follow…ExpressionMammalianAvailable SinceApril 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLNHA-C1-HseIF4AI_K
Plasmid#146731PurposeMammalian Expression of HseIF4AIDepositorInsertHseIF4AI (EIF4A1 Human)
ExpressionMammalianMutationa non silent mutation (A200V) and 3 silent mutati…Available SinceMay 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pT7-V5-SBP-C1-HsUpf1_1-925-siRNAres_P
Plasmid#147191PurposeMammalian Expression of HsUpf1_1-925-siRNAresDepositorInsertHsUpf1_1-925-siRNAres (UPF1 Human)
ExpressionMammalianMutation4 non silent mutations A69S, E167G, K282R and A4…Available SinceJan. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pT7-V5-SBP-C1-HsUpf1-del296-926_P
Plasmid#147195PurposeMammalian Expression of HsUpf1-del296-926DepositorInsertHsUpf1-del296-926 (UPF1 Human)
ExpressionMammalianMutation4 non silent mutations A69S, E167G, K282R and T1…Available SinceJan. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pT7-V5-SBP-C1-HsUpf1_115-1129-siRNAres_P
Plasmid#147193PurposeMammalian Expression of HsUpf1_115-1129-siRNAresDepositorInsertHsUpf1_115-1129-siRNAres (UPF1 Human)
ExpressionMammalianMutation5 non silent mutations A69S, E167G, K282R, A442G…Available SinceMarch 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pT7-V5-SBP-C1-HsUpf1-V161ER162E-siRNAres_Q
Plasmid#147332PurposeMammalian Expression of HsUpf1-V161ER162EDepositorInsertHsUpf1-V161ER162E (UPF1 Human)
ExpressionMammalianMutation5 non silent mutations A69S, E167G, K282R, A442G…Available SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDONOR_MM001-ZFP143-FKBPV-2HA-P2A-GFP-targetting construct
Plasmid#212705Purposetargeting vector to introduce an FKBPV-2HA-P2A-GFP sequence at the C terminal of Zfp143 locus . Degradation tag (dTAG) systemDepositorInsertZFP143-FKBPV-2HA-P2A-GFP
Available SinceDec. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSMH1741
Plasmid#160930PurposeExpression of soluble Sc octamer (H3)DepositorInsertsHHF1
HTA1
HTB1
HHT1
Tags6His-TEV, N-6His-TEV, N-terminal 6His-TEV, and no…Mutationcodon-optimized for E. coli -- see provided seque…Available SinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSMH1742
Plasmid#160929PurposeExpression of soluble Sc octamer (Cse4)DepositorInsertsHHF1
HTA1
HTB1
Cse4
Tags6His-TEV, N-terminal 6His-TEV, and noneMutationcodon-optimized for E. coli -- see provided seque…Available SinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLEW-pHluorin2-PTS1
Plasmid#213776PurposeExpresses the fluorescent protein pH sensor pHlorin2 in the glycosomes of Trypanosomal bruceiDepositorInsertpHlorin2
UseTrypanosoma brucei expressionMutationAppended a 2 glycine linker and a T. brucei perox…PromoterT. brucei rRNA promotorAvailable SinceFeb. 21, 2024AvailabilityAcademic Institutions and Nonprofits only