We narrowed to 27,276 results for: Tat

1. pcDNA5/FRT/TO DNAJB10

   Plasmid

   #19479

   Depositor

   Harm Kampinga

   Article

   Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

   Insert

   DNAJB10 (DNAJB2 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 7, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEN_TmiR_G12-E

   Plasmid

   #25761

   Purpose

   Entry vector with TRE promoter driving mouse G alpha 12 miR30-based shRNA.

   Depositor

   Iain Fraser

   Article

   Shin et al Proc Natl Acad Sci U S A. 2006 Sep 12. 1

   Insert

   G alpha 12 miR-shRNA (Gna12 Mouse)
   

   Use

   Entry vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 14, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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3. Lenti NG-ABE8e_P2A_GFP + PuroR

   Plasmid

   #242000

   Purpose

   Lentiviral plasmid for generation of cell lines stably expressing NG-ABE8e base editor. NG-ABE8e expression can be followed by GFP fluorescence. Contains PGK-puromycin resistance cassette.

   Depositor

   Frank Buchholz

   Article

   Wang et al Genome Biol. 2025 Jul 22;26(1):217. doi:

   Insert

   NG-ABE8e
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 25, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV_CaMKII-RFP-p2A-DAAO(R285A)-NES

   Plasmid

   #238919

   Purpose

   Mammalian expression of mutated DAAO(R285A) with a nuclear export signal and RFP as a reporter in excitatory neurons

   Depositor

   Vsevolod Belousov

   Article

   Kalinichenko et al Redox Biol. 2023 Apr;60:102604. doi: 10.

   Insert

   RFP-p2A-DAAO(R285A)-NES
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CaMKIIalfa

   Available since

   Aug. 19, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pdU6-2_sgRNA1-5-HT2AR-isoformBFH

   Plasmid

   #221825

   Purpose

   Plasmid to express gRNA1 (cgctatcggtctgtgacaga) for editing at the end of Drosophila 5-HT2AR isoforms B, F and H coding frame

   Depositor

   Quentin Gaudry

   Article

   Mallick et al bioRxiv [Preprint]. 2024 Apr 14:2024.04.

   Insert

   d5-HT2AR isoforms BFH gRNA1 (5-HT2 Fly)
   

   Use

   CRISPR

   Tags

   Expression

   Insect

   Mutation

   Promoter

   zebrafish U6 promoter (U6b)

   Available since

   Aug. 18, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAD100-CHIP-myc-His

   Plasmid

   #236054

   Purpose

   Mammalian expression of human CHIP with a C-terminal myc-His tag

   Depositor

   Jonathan Schisler

   Article

   Stewart et al bioRxiv 2025.02.17.637997

   Insert

   CHIP (STUB1 Human)
   

   Use

   Tags

   myc-His

   Expression

   Mammalian

   Mutation

   none

   Promoter

   CMV

   Available since

   Aug. 7, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. VAMP4 KD1 mScarlet

   Plasmid

   #174407

   Purpose

   lentiviral vector for VAMP4 knock-down in rat cells, KD1 shRNA

   Depositor

   David Perrais

   Article

   Bakr et al Cell Rep. 2021 Sep 7;36(10):109678. doi:

   Insert

   vesicle associated membrane protein 4 (Vamp4 Rat)
   

   Use

   Lentiviral

   Tags

   mScarlet

   Expression

   Mammalian

   Mutation

   Promoter

   H1-Ubiquitin

   Available since

   Aug. 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCAGIG-Sema7A-KCE

   Plasmid

   #190651

   Purpose

   Expresses Mouse Sema7A with RGD motif mutated to KCE

   Depositor

   Victor Tarabykin, Andrew Newman

   Article

   Bessa et al Nat Commun. 2024 Aug 16;15(1):7059. doi:

   Insert

   Sema7A (Sema7a Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   RGD mutated to KCE

   Promoter

   Available since

   July 23, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pX459-SOS1(dog)-gRNA1

   Plasmid

   #228755

   Purpose

   A knockout vector for dog SOS1.

   Depositor

   Michiyuki Matsuda

   Article

   Jikko et al J Cell Sci. 2025 Jul 16:jcs.263779. doi:

   Insert

   A gRNA targeting the dog SOS1 gene and the cDNA of CRISPR-Cas9 (SOS1 canis lupus)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 22, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCAD130_hIrg1_M154I_pvp008

    Plasmid

    #239117

    Purpose

    hACOD1 with M154I mutation in E.coli expression vector

    Depositor

    Konrad Buessow

    Article

    Chen et al Sci Rep. 2023 Jun 26;13(1):10360. doi: 1

    Insert

    cis-aconitate decarboxylase (ACOD1 Human)
    

    Use

    Tags

    StrepTag-II

    Expression

    Bacterial

    Mutation

    M154I

    Promoter

    T7

    Available since

    July 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCAD131_hIrg1_M154I_pCMV6

    Plasmid

    #239120

    Purpose

    hACOD1 with M154I mutation in mammalian expression vector

    Depositor

    Konrad Buessow

    Article

    Chen et al Sci Rep. 2023 Jun 26;13(1):10360. doi: 1

    Insert

    cis-aconitate decarboxylase (ACOD1 Human)
    

    Use

    Tags

    Myc and FLAG

    Expression

    Mammalian

    Mutation

    M154I

    Promoter

    CMV

    Available since

    July 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMT-sfGFP-TurboID-ER

    Plasmid

    #240230

    Purpose

    Expression of ER-localized sfGFP-TurboID under control of copper-inducible promoter. Can be used to generate stable cell lines by Hygromycin selection.

    Depositor

    Norbert Perrimon, Justin Bosch

    Article

    Bosch et al bioRxiv 2025.07.09.659702; doi: https://

    Insert

    BIP-sfGFP-TurboID-KDEL
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    MT

    Available since

    July 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMT-sfGFP-TurboID

    Plasmid

    #240231

    Purpose

    Expression of sfGFP-TurboID under control of copper-inducible promoter. Can be used to generate stable cell lines by Hygromycin selection.

    Depositor

    Norbert Perrimon, Justin Bosch

    Article

    Bosch et al bioRxiv 2025.07.09.659702; doi: https://

    Insert

    sfGFP-TurboID
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    MT

    Available since

    July 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMT-Myc-BirA*G3-ER

    Plasmid

    #240234

    Purpose

    Expression of ER-localized Myc-BirA*-G3 under control of copper-inducible promoter. Can be used to generate stable cell lines by Hygromycin selection.

    Depositor

    Norbert Perrimon, Justin Bosch

    Article

    Bosch et al bioRxiv 2025.07.09.659702; doi: https://

    Insert

    BiP-Myc-BirA*G3-KDEL
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    MT

    Available since

    July 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pWal10-roe-sfGFP

    Plasmid

    #240239

    Purpose

    Gateway destination plasmid to generate C-terminal sfGFP fusions under control of UAS promoter. Can be used to generate transgenic fly lines by white+ fly eye selection and phiC31 integration.

    Depositor

    Norbert Perrimon, Justin Bosch

    Article

    Bosch et al bioRxiv 2025.07.09.659702; doi: https://

    Type

    Empty backbone

    Use

    Gateway destination vector

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    July 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pENTR_spatzle-HA

    Plasmid

    #240227

    Purpose

    Gateway entry clone with spatzle tagged with HA (contains stop codon)

    Depositor

    Norbert Perrimon, Justin Bosch

    Article

    Bosch et al bioRxiv 2025.07.09.659702; doi: https://

    Insert

    spatzle (spz Fly)
    

    Use

    Gateway shuttle vector

    Tags

    HA-spGFP11

    Expression

    Mutation

    Promoter

    Available since

    July 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pHAGE2-TetO-SOX17WT

    Plasmid

    #216176

    Purpose

    Generate lentiviruses encoding for wild-type SOX17

    Depositor

    Ralf Jauch

    Article

    Ho et al Stem Cell Reports. 2025 Mar 11;20(3):102

    Insert

    SOX17 (SOX17 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Glu113Glu (silent mutation); Glu120Glu (silent mu…

    Promoter

    Available since

    July 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pRRL-SFFV_V5-CHIC2-C6S_Tomato

    Plasmid

    #238128

    Purpose

    Expresses V5-tagged CHIC2 (with C6S mutation) with Tomato selectable marker.

    Depositor

    Arlene Sharpe

    Article

    LaFleur et al Nat Immunol. 2025 Sep;26(9):1476-1487. d

    Insert

    V5-tagged CHIC2 with C6S mutation
    

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Changed cysteines from amino acids 102-109 to ser…

    Promoter

    SFFV

    Available since

    July 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pRCA905 - KLF4 Puro-T2A-BFP (pRCA847 backbone)

    Plasmid

    #238185

    Purpose

    Lentiviral vector for overexpression KLF4 ORF from a EF1a promoter

    Depositor

    Thomas Norman

    Article

    Southard et al Nat Genet. 2025 Aug 6. doi: 10.1038/s415

    Insert

    KLF4 (KLF4 Human, Synthetic)
    

    Use

    Lentiviral

    Tags

    mTagBFP2

    Expression

    Mutation

    Promoter

    Available since

    June 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pRCA879 - KLF5 isoform 1 Puro-T2A-GFP (pRCA845 backbone)

    Plasmid

    #238181

    Purpose

    Lentiviral vector for overexpression KLF5 ORF from a EF1a promoter

    Depositor

    Thomas Norman

    Article

    Southard et al Nat Genet. 2025 Aug 6. doi: 10.1038/s415

    Insert

    KLF5 (KLF5 Human, Synthetic)
    

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Mutation

    Promoter

    Available since

    June 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pRCA903 - KLF5 isoform 1 Puro-T2A-BFP (pRCA845 backbone)

    Plasmid

    #238184

    Purpose

    Lentiviral vector for overexpression KLF5 ORF from a EF1a promoter

    Depositor

    Thomas Norman

    Article

    Southard et al Nat Genet. 2025 Aug 6. doi: 10.1038/s415

    Insert

    KLF5 (KLF5 Human, Synthetic)
    

    Use

    Lentiviral

    Tags

    mTagBFP2

    Expression

    Mutation

    Promoter

    Available since

    June 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pRCA881 - KLF4 Puro-T2A-GFP (pRCA847 backbone)

    Plasmid

    #238182

    Purpose

    Lentiviral vector for overexpression KLF4 ORF from a EF1a promoter

    Depositor

    Thomas Norman

    Article

    Southard et al Nat Genet. 2025 Aug 6. doi: 10.1038/s415

    Insert

    KLF4 (KLF4 Human, Synthetic)
    

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Mutation

    Promoter

    Available since

    June 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pGEMHE-MmBorealin-OLLAS

    Plasmid

    #237439

    Purpose

    Expresses mouse Borealin tagged with OLLAS at C-term; made for in vitro transcription (T7 promoter)

    Depositor

    Michael Lampson

    Article

    Dudka et al Nature. 2025 Feb;638(8051):814-822. doi:

    Insert

    Borealin (Cdca8 Mouse)
    

    Use

    Tags

    OLLAS

    Expression

    Mammalian

    Mutation

    Promoter

    T7

    Available since

    June 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pk335-CARGO-6mer-73kb-DSF

    Plasmid

    #227499

    Purpose

    6-mer gRNA array targeting Sp dCas9/Cas9 to the Prdm8-Fgf5 locus

    Depositor

    Joanna Wysocka

    Article

    Jensen et al Mol Cell. 2025 Jan 16;85(2):347-361.e7.

    Insert

    CARGO to 73kb Downstream Fgf5
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pk335-CARGO-4mer-35kb-DSF

    Plasmid

    #227491

    Purpose

    4-mer gRNA array targeting Sp dCas9/Cas9 to the Prdm8-Fgf5 locus

    Depositor

    Joanna Wysocka

    Article

    Jensen et al Mol Cell. 2025 Jan 16;85(2):347-361.e7.

    Insert

    CARGO to 35kb Downstream Fgf5
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pk335-CARGO-6mer-7.1kb-USF

    Plasmid

    #227474

    Purpose

    6-mer gRNA array targeting Sp dCas9/Cas9 to the Prdm8-Fgf5 locus

    Depositor

    Joanna Wysocka

    Article

    Jensen et al Mol Cell. 2025 Jan 16;85(2):347-361.e7.

    Insert

    CARGO to 7.1kb Upstream Fgf5
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pk335-CARGO-6mer-3.3kb-USP

    Plasmid

    #227450

    Purpose

    6-mer gRNA array targeting Sp dCas9/Cas9 to the Prdm8-Fgf5 locus

    Depositor

    Joanna Wysocka

    Article

    Jensen et al Mol Cell. 2025 Jan 16;85(2):347-361.e7.

    Insert

    CARGO to 3.3kb Upstream Prdm8
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pk335-CARGO-6mer-58kb-USF

    Plasmid

    #227457

    Purpose

    6-mer gRNA array targeting Sp dCas9/Cas9 to the Prdm8-Fgf5 locus

    Depositor

    Joanna Wysocka

    Article

    Jensen et al Mol Cell. 2025 Jan 16;85(2):347-361.e7.

    Insert

    CARGO to 58kb Upstream Fgf5
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pk335-CARGO-6mer-40kb-USF

    Plasmid

    #227460

    Purpose

    6-mer gRNA array targeting Sp dCas9/Cas9 to the Prdm8-Fgf5 locus

    Depositor

    Joanna Wysocka

    Article

    Jensen et al Mol Cell. 2025 Jan 16;85(2):347-361.e7.

    Insert

    CARGO to 40kb Upstream Fgf5
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pk335-CARGO-6mer-36kb-USF

    Plasmid

    #227461

    Purpose

    6-mer gRNA array targeting Sp dCas9/Cas9 to the Prdm8-Fgf5 locus

    Depositor

    Joanna Wysocka

    Article

    Jensen et al Mol Cell. 2025 Jan 16;85(2):347-361.e7.

    Insert

    CARGO to 36kb Upstream Fgf5
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pk335-CARGO-5mer-36kb-USF

    Plasmid

    #227462

    Purpose

    5-mer gRNA array targeting Sp dCas9/Cas9 to the Prdm8-Fgf5 locus

    Depositor

    Joanna Wysocka

    Article

    Jensen et al Mol Cell. 2025 Jan 16;85(2):347-361.e7.

    Insert

    CARGO to 36kb Upstream Fgf5
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pk335-CARGO-6mer-33kb-USF

    Plasmid

    #227465

    Purpose

    6-mer gRNA array targeting Sp dCas9/Cas9 to the Prdm8-Fgf5 locus

    Depositor

    Joanna Wysocka

    Article

    Jensen et al Mol Cell. 2025 Jan 16;85(2):347-361.e7.

    Insert

    CARGO to 33kb Upstream Fgf5
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pTMiniTrex-mCherry-Shark3

    Plasmid

    #233145

    Purpose

    Serves as a template plasmid for amplifying Shark3 (Theo-OFF) hammerhead aptazyme-3xTy-tagging cassette

    Depositor

    Ronald Etheridge

    Article

    Wiedeman et al Proc Natl Acad Sci U S A. 2025 Feb 25;12

    Inserts

    mCherry
    Shark3
    

    Use

    Prokaryotic expression

    Tags

    3x Ty tag

    Expression

    Mutation

    Codon optimized for Trypanosoma cruzi and changed…

    Promoter

    Available since

    March 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pJExpress401-KDAC8 D101N

    Plasmid

    #224348

    Purpose

    Expresses human KDAC8 (HDAC8) D101N in E. coli

    Depositor

    Terry Watt

    Article

    Toro et al Biochemistry. 2021 Aug 24;60(33):2524-25

    Insert

    KDAC8 (HDAC8 Human)
    

    Use

    Tags

    TEV-cleavable His6

    Expression

    Bacterial

    Mutation

    Aspartate 101 mutated to asparagine

    Promoter

    T5

    Available since

    Feb. 19, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

35. pGL3_5UTR_Myc_FLuc Compensatory Mutant

    Plasmid

    #229504

    Purpose

    Myc 5' untranslated region firefly luciferase reporter with compensatory mutations in the region bound by RBM42

    Depositor

    Davide Ruggero

    Article

    Kovalski et al Nat Cell Biol. 2025 Feb 4. doi: 10.1038/

    Insert

    Myc 5'UTR_CompMutant
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Mutations in bp 363-394

    Promoter

    SV40

    Available since

    Feb. 12, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pPB9v2

    Plasmid

    #210029

    Purpose

    Contains cloning site for molecularly-tagged Cas9 gRNA constructs, inducible Cas9, rtTA-T2A-puro

    Depositor

    Michael Talkowski

    Article

    Nuttle et al Cell Rep Methods. 2023 Dec 5:100672. doi

    Inserts

    Cas9
    rtta-T2A-puro
    

    Use

    CRISPR

    Tags

    SV40 NLS and nucleoplasmin NLS

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1α and TRE3G

    Available since

    Feb. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pPB9P2

    Plasmid

    #210030

    Purpose

    Contains PE2 prime editing gRNA cloning site, inducible prime editor 2, rtTA-T2A-puro

    Depositor

    Michael Talkowski

    Article

    Nuttle et al Cell Rep Methods. 2023 Dec 5:100672. doi

    Inserts

    prime editor 2
    rtta-T2A-puro
    

    Use

    CRISPR

    Tags

    SV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1α and TRE3G

    Available since

    Feb. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pPB758

    Plasmid

    #210033

    Purpose

    Contains inducible Cas7-P2A-Cas5-T2A-Cas8, rtTA-T2A-puro

    Depositor

    Michael Talkowski

    Article

    Nuttle et al Cell Rep Methods. 2023 Dec 5:100672. doi

    Inserts

    Cas7-P2A-Cas5-T2A-Cas8
    rtta-T2A-puro
    

    Use

    CRISPR

    Tags

    SV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1α and TRE3G

    Available since

    Feb. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pCS2+ 3xFLAG-FEM1B C186S

    Plasmid

    #230995

    Purpose

    Transient overexpression of mouse FEM1B in mammalian cells. Mutation abolishes substrate binding.

    Depositor

    Michael Rape

    Article

    Manford et al Cell. 2020 Oct 1;183(1):46-61.e21. doi:

    Insert

    FEM1B (Fem1b Mouse)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    mutated Cys186 to Ser

    Promoter

    Available since

    Jan. 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pCS2+ 3xFLAG-FEM1B L597A

    Plasmid

    #230994

    Purpose

    Transient overexpression of catalytic null mouse FEM1B in mammalian cells. Mutation abolishes interaction with CUL2.

    Depositor

    Michael Rape

    Article

    Manford et al Cell. 2020 Oct 1;183(1):46-61.e21. doi:

    Insert

    FEM1B (Fem1b Mouse)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    mutated Leu597 to Ala

    Promoter

    Available since

    Jan. 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge