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pECE-M2-CDC27 S379A

Plasmid

#31662

Depositor

Anne Brunet

Article

Banko et al Mol Cell. 2011 Dec 23;44(6):878-92. Epub

Insert

cell division cycle 27 homolog S379A (CDC27 Human)

Tags

M2

Expression

Mammalian

Mutation

changed Serine 379 to Alanine

Available Since

April 11, 2012

Availability

Academic Institutions and Nonprofits only
pGEX-CDC27 S379A

Plasmid

#31674

Depositor

Anne Brunet

Article

Banko et al Mol Cell. 2011 Dec 23;44(6):878-92. Epub

Insert

cell division cycle 27 homolog S379A (CDC27 Human)

Tags

GST

Expression

Bacterial

Mutation

changed Serine 379 to Alanine

Available Since

Feb. 17, 2012

Availability

Academic Institutions and Nonprofits only
pSico-syn-GFP-TVA-oG-WPRE-bGHpA

Plasmid

#231009

Purpose

Lentiviral helper virus for G-deleted rabies tracing

Depositor

Tomasz Nowakowski

Article

Shin et al bioRxiv [Preprint]. 2024 Dec 11:2024.10.

Inserts

Enhanced GFP
TVA (CD320 Chicken)
optimized G

Use

Lentiviral

Promoter

human synapsin I

Available Since

Dec. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV hSyn INTRON HaloTag-STIM1 WPRE

Plasmid

#236233

Purpose

AAV expression of mouse STIM1 internally tagged with HaloTag

Depositor

Jennifer Lippincott-Schwartz

Article

Benedetti et al Cell. 2025 Jan 23;188(2):484-500.e22. do

Insert

HaloTag-Stim1 (Stim1 Mouse)

Use

AAV

Tags

HaloTag

Promoter

human Synapsin 1

Available Since

May 2, 2025

Availability

Academic Institutions and Nonprofits only

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pEMS2156

Plasmid

#158099

Purpose

High Copy number plasmid containing SYN1 insert

Depositor

Elizabeth Simpson

Article

de Moura Gomes et al Sci Rep. 2024 Nov 15;14(1):28194. doi: 1

Insert

SYN1 (SYN1 Human)

Promoter

SYN1

Available Since

Nov. 18, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pOTTC1663 - pAAV SYN1 mGas6-Myc-DDK

Plasmid

#202540

Purpose

An adeno-associated viral vector expressing murine Gas6 fused Myc and DDK epitopes from a synapsin promoter

Depositor

Christopher Richie

Article

Owlett et al J Neuroinflammation. 2022 Feb 7;19(1):38

Insert

Gas6 (Gas6 Mouse)

Use

AAV

Tags

Myc-DDK

Promoter

SYN1

Available Since

June 20, 2023

Availability

Academic Institutions and Nonprofits only

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pnCS_SEPT7_SEPT9_i1-i5-TEV-Strep

Plasmid

#180314

Purpose

bacterial co-expression of human SEPT7 and of human SEPT9_i1-i5

Depositor

Manos Mavrakis

Article

Kuzmic et al J Cell Sci. 2021 Dec 2. pii: 273604. doi

Inserts

SEPTIN7 (SEPTIN7 Human)
SEPTIN9 (SEPTIN9 )

Tags

TEV-Strep

Expression

Bacterial

Mutation

SEPT9_i1-i5

Available Since

March 30, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV_SEPT9_i1 S22A S23A-msfGFP

Plasmid

#180338

Purpose

mammalian expression of human SEPT9_i1 S22A S23A fused to monomeric superfolder GFP

Depositor

Manos Mavrakis

Article

Kuzmic et al J Cell Sci. 2021 Dec 2. pii: 273604. doi

Insert

SEPTIN9_v1 (SEPTIN9 Human)

Tags

msfGFP

Expression

Mammalian

Mutation

SEPT9_i1 S22A S23A

Promoter

CMV

Available Since

March 29, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV_SEPT9_i1 S12A S13A-msfGFP

Plasmid

#180337

Purpose

mammalian expression of human SEPT9_i1 S12A S13A fused to monomeric superfolder GFP

Depositor

Manos Mavrakis

Article

Kuzmic et al J Cell Sci. 2021 Dec 2. pii: 273604. doi

Insert

SEPTIN9_v1 (SEPTIN9 Human)

Tags

msfGFP

Expression

Mammalian

Mutation

SEPT9_i1 S12A S13A

Promoter

CMV

Available Since

March 29, 2022

Availability

Academic Institutions and Nonprofits only

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TMD18

Plasmid

#163673

Purpose

Expresses a ST6GAL1 mutant with 18 amino acids in its TMD in mammalian cells

Depositor

Lei Lu

Article

Sun et al J Cell Sci. 2021 Oct 15;134(20). pii: 27

Insert

partial length ST6 beta-galactoside alpha-2,6-sialyltransferase 1 mutant (ST6GAL1 Human)

Tags

GFP

Expression

Mammalian

Mutation

For this partial length ST chimera, two restricti…

Available Since

Nov. 24, 2021

Availability

Academic Institutions and Nonprofits only

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TMD19

Plasmid

#163674

Purpose

Expresses a ST6GAL1 mutant with 19 amino acids in its TMD in mammalian cells

Depositor

Lei Lu

Article

Sun et al J Cell Sci. 2021 Oct 15;134(20). pii: 27

Insert

partial length ST6 beta-galactoside alpha-2,6-sialyltransferase 1 mutant (ST6GAL1 Human)

Tags

GFP

Expression

Mammalian

Mutation

For this partial length ST chimera, two restricti…

Available Since

Nov. 24, 2021

Availability

Academic Institutions and Nonprofits only

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TMD20

Plasmid

#163675

Purpose

Expresses a ST6GAL1 mutant with 20 amino acids in its TMD in mammalian cells

Depositor

Lei Lu

Article

Sun et al J Cell Sci. 2021 Oct 15;134(20). pii: 27

Insert

partial length ST6 beta-galactoside alpha-2,6-sialyltransferase 1 mutant (ST6GAL1 Human)

Tags

GFP

Expression

Mammalian

Mutation

For this partial length ST chimera, two restricti…

Available Since

Nov. 24, 2021

Availability

Academic Institutions and Nonprofits only

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TMD21

Plasmid

#163676

Purpose

Expresses a ST6GAL1 mutant with 21 amino acids in its TMD in mammalian cells

Depositor

Lei Lu

Article

Sun et al J Cell Sci. 2021 Oct 15;134(20). pii: 27

Insert

partial length ST6 beta-galactoside alpha-2,6-sialyltransferase 1 mutant (ST6GAL1 Human)

Tags

GFP

Expression

Mammalian

Mutation

For this partial length ST chimera, two restricti…

Available Since

Nov. 24, 2021

Availability

Academic Institutions and Nonprofits only

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TMD22

Plasmid

#163677

Purpose

Expresses a ST6GAL1 mutant with 22 amino acids in its TMD in mammalian cells

Depositor

Lei Lu

Article

Sun et al J Cell Sci. 2021 Oct 15;134(20). pii: 27

Insert

partial length ST6 beta-galactoside alpha-2,6-sialyltransferase 1 mutant (ST6GAL1 Human)

Tags

GFP

Expression

Mammalian

Mutation

For this partial length ST chimera, two restricti…

Available Since

Nov. 24, 2021

Availability

Academic Institutions and Nonprofits only

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TMD24

Plasmid

#163678

Purpose

Expresses a ST6GAL1 mutant with 24 amino acids in its TMD in mammalian cells

Depositor

Lei Lu

Article

Sun et al J Cell Sci. 2021 Oct 15;134(20). pii: 27

Insert

partial length ST6 beta-galactoside alpha-2,6-sialyltransferase 1 mutant (ST6GAL1 Human)

Tags

GFP

Expression

Mammalian

Mutation

For this partial length ST chimera, two restricti…

Available Since

Nov. 24, 2021

Availability

Academic Institutions and Nonprofits only

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FKBP-ST*-GFP

Plasmid

#163669

Purpose

Expresses FKBP tagged partial length ST in mammalian cells

Depositor

Lei Lu

Article

Sun et al J Cell Sci. 2021 Oct 15;134(20). pii: 27

Insert

partial length ST6 beta-galactoside alpha-2,6-sialyltransferase 1 (first 113 amino acids) (ST6GAL1 Human)

Tags

FK506 binding protein (FKBP) and GFP

Expression

Mammalian

Mutation

This chimera contains the first 113 amino acids o…

Available Since

Nov. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV hSyn INTRON mScarlet-CAAX2 WPRE

Plasmid

#236232

Purpose

AAV expression of a fluorescent marker, mScarletI, fused to the plasma membrane targeting sequence CAAX2

Depositor

Jennifer Lippincott-Schwartz

Article

Benedetti et al Cell. 2025 Jan 23;188(2):484-500.e22. do

Insert

mScarletI fused to the plasma membrane targeting sequence CAAX2

Use

AAV

Tags

mScarletI

Promoter

human Synapsin 1

Available Since

May 1, 2025

Availability

Academic Institutions and Nonprofits only

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hKCNQ5(WT).ires.GFP-pcDNA3

Plasmid

#204360

Purpose

Heterologous expression in mammalian cell lines or Xenopus oocyte (T7 RNA pol)

Depositor

Aguan Wei

Article

Wei et al J Neurophysiol. 2022 Jul 1;128(1):40-61.

Insert

KCNQ5 (KCNQ5 Human)

Expression

Mammalian

Promoter

CMV/T7 RNApol

Available Since

Aug. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV hSyn INTRON mTagBFP2-CAAX2 WPRE

Plasmid

#236231

Purpose

AAV expression of a fluorescent marker, mTagBFP2, fused to the plasma membrane targeting sequence CAAX2

Depositor

Jennifer Lippincott-Schwartz

Article

Benedetti et al Cell. 2025 Jan 23;188(2):484-500.e22. do

Insert

mTagBFP2 fused to the plasma membrane targeting sequence CAAX2

Use

AAV

Tags

mTagBFP2

Promoter

human Synapsin 1

Available Since

May 1, 2025

Availability

Academic Institutions and Nonprofits only

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pGP-AAV-syn-FLEX-jGCaMP8m-WPRE

Plasmid

#162378

Purpose

AAV-mediated expression of ultrafast protein calcium sensor under the Syn promoter, Cre-dependent expression

Depositor

GENIE Project

Article

Zhang et al Janelia Research Campus, Online Resource

Has Service

AAV Retrograde, AAV1, AAV5, and AAV9

Insert

jGCaMP8m

Use

AAV and Cre/Lox

Tags

6xHis

Expression

Mammalian

Mutation

A25G F286Y

Promoter

Synapsin

Available Since

Nov. 30, 2020

Availability

Academic Institutions and Nonprofits only

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