We narrowed to 5,566 results for: SPI

1. 7432 Bicistronic_GFP_ires_puro

   Plasmid

   #64336

   Purpose

   This a retroviral expression plasmid expressing GFP along with puro resistance gene

   Depositor

   Georgios Stathopoulos

   Article

   Agalioti et al Nat Commun. 2017 May 16;8:15205. doi: 10

   Insert

   GFP
   

   Use

   Retroviral

   Tags

   GFP

   Expression

   Mutation

   Promoter

   Available since

   July 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pVenus-CyclinB1(R42A) (1317)

   Plasmid

   #39873

   Depositor

   Jonathon Pines

   Article

   Hagting et al J Cell Biol. 2002 Jun 24;157(7):1125-37.

   Insert

   CyclinB1(R42A) (CCNB1 Human)
   

   Use

   Tags

   Venus

   Expression

   Mammalian

   Mutation

   R42A = destruction box mutations = non-destructib…

   Promoter

   Available since

   Oct. 31, 2012

   Availability

   Academic Institutions and Nonprofits only

3. pcDNA5-FRT/TO-neo-C11orf51-IRES2-mRuby (2763)

   Plasmid

   #39858

   Depositor

   Jonathon Pines

   Article

   Mansfeld et al Nat Cell Biol. 2011 Sep 18;13(10):1234-4

   Insert

   C11orf51 (ANAPC15 Human)
   

   Use

   Tags

   IRES2-mRuby

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 31, 2012

   Availability

   Academic Institutions and Nonprofits only

4. AfrLEA3m_mCh-2xFKBP (pBS1137)

   Plasmid

   #185321

   Purpose

   For the mammalian expression of the brine shrimp protein AfrLEA3m attached to 2xFKBP. The FKBP can be used for inducible dimerization and condensate formation

   Depositor

   Pamela Silver

   Article

   Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

   Insert

   AfrLEA3m
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pST_APOE_HUMAN_E4_47-300 (pBS0847)

   Plasmid

   #185279

   Purpose

   For the mammalian expression of the human protein APOE_HUMAN_E4_47-300. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis).

   Depositor

   Pamela Silver

   Article

   Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

   Insert

   APOE_HUMAN_E4_47-300
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMSCV-puro-AS9 (Mito MTS)-Delta N67-mMCL1

   Plasmid

   #45822

   Depositor

   Joseph Opferman

   Article

   Perciavalle et al Nat Cell Biol. 2012 Apr 29;14(6):575-83.

   Insert

   AS9(Mito MTS)-Delta N67-mMCL1
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   ATP Synthase subunit 9 is used as a Mito Targetin…

   Promoter

   Available since

   July 24, 2013

   Availability

   Academic Institutions and Nonprofits only

7. pETDuet-1-6xHis-TEV-Nsp8

   Plasmid

   #201022

   Purpose

   Bacterial expression of codon optimized SARS-CoV-2 nsp8 tagged with an N-terminus His-tag followed by a TEV protease cleavage site.

   Depositor

   Sharon Rozovsky

   Article

   Ghelichkhani et al J Mol Biol. 2023 Apr 15;435(8):168008. d

   Insert

   non-structural protein 8 (ORF1ab Severe acute respiratory syndrome coronavirus 2, Synthetic)
   

   Use

   Tags

   6xHis-TEV

   Expression

   Bacterial

   Mutation

   Gene insert is codon optimized for Ecoli.

   Promoter

   T7/LacO

   Available since

   May 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pHEN-CoV2-nanobody-Ty1

   Plasmid

   #194588

   Purpose

   Bacterial expression of Ty1 nanobody targeting the receptor binding domain (RBD) of SARS-CoV-2 spike

   Depositor

   Gerald McInerney

   Article

   Hanke et al Nat Commun. 2020 Sep 4;11(1):4420. doi:

   Insert

   Ty1 (S )
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-hSyn-sbGLuc-CheRiff-EGFP

   Plasmid

   #114108

   Purpose

   fusion protein of Gaussia luciferase variant slow burn, synthetic channelrhodopsin CheRiff, and enhanced green fluorescent protein for bioluminescent optogenetics

   Depositor

   Ute Hochgeschwender

   Article

   Ikefuama et al Int J Mol Sci. 2022 Oct 27;23(21):12994.

   Insert

   Gaussia luciferase, slow burn; CheRiff synthetic ChR; EGFP
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hSyn

   Available since

   Aug. 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. CAHS2parrc-mCh-2xFKBP (pBS1109)

    Plasmid

    #185304

    Purpose

    For the mammalian expression of the tardigrade protein CAHS2parrc attached to 2xFKBP. The FKBP can be used for inducible dimerization and condensate formation

    Depositor

    Pamela Silver

    Article

    Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

    Insert

    CAHS2parrc
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. MBP-GTSE1(long)-His6

    Plasmid

    #69110

    Purpose

    For bacterial expression of recombinant human GTSE1 (longer isoform) tagged with MBP and His.

    Depositor

    Stephen Royle

    Article

    Nixon et al Elife. 2015 Jun 19;4. doi: 10.7554/eLife

    Insert

    G2- and S-phase Expressed 1 (GTSE1 Human)
    

    Use

    Tags

    His6 and MBP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGY416

    Plasmid

    #166660

    Purpose

    For light-inducible Cre recombinase (LiCre) expression under the Gal1 promoter in yeast

    Depositor

    Gaël Yvert

    Article

    Duplus-Bottin et al Elife. 2021 Feb 23;10. pii: 61268. doi:

    Insert

    LiCre
    

    Use

    Cre/Lox

    Tags

    Expression

    Yeast

    Mutation

    Cre: E340A, D341A

    Promoter

    GAL1

    Available since

    April 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. Topo_SpCas9.tracr_LbCas12a.DR

    Plasmid

    #155049

    Purpose

    TOPO vector for the cloning of _the SpCas9 tracrRNA - LbCas12a Direct Repeat (DR) fragment into the pLCHKO hgRNA vector

    Depositor

    Benjamin Blencowe, Jason Moffat

    Article

    Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.

    Insert

    SpCas9 tracrRNA and LbCas12a Direct Repeat (DR)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLenti6.2-coHPDL WT 3xFlag-V5

    Plasmid

    #174129

    Purpose

    Expresses codon-optimized HPDL with a 3xFlag-V5 tag in mammalian cells

    Depositor

    Michael Pacold

    Article

    Banh et al Nature. 2021 Sep 1. pii: 10.1038/s41586-

    Insert

    4-hydroxyphenylpyruvate dioxygenase like (HPDL Human)
    

    Use

    Lentiviral

    Tags

    3xFLAG-V5

    Expression

    Mammalian

    Mutation

    Codon optimized

    Promoter

    Available since

    Sept. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pST_MAHS_RAMVA_81-236-FLAG (pBS0297)

    Plasmid

    #185328

    Purpose

    For the mammalian expression of the tardigrade protein MAHS_RAMVA_81-236-FLAG. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis).

    Depositor

    Pamela Silver

    Article

    Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

    Insert

    MAHS_RAMVA_81-236-FLAG
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. CRY-tTA (B702)

    Plasmid

    #92032

    Purpose

    Expresses CRY2 (Full length, mutated NLS) fusion with tTA2 'tet-OFF' transcriptional activator

    Depositor

    Chandra Tucker

    Article

    Pathak et al Nucleic Acids Res. 2017 Apr 20. doi: 10.

    Insert

    CRY2 (CRY2 Mustard Weed)
    

    Use

    Tags

    tTA2 (TetR fused to VP64)

    Expression

    Mammalian

    Mutation

    NLS on CRY2 is mutated

    Promoter

    CMV

    Available since

    July 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pEGFP-PH-SWAP70(R223E, R224E)

    Plasmid

    #84583

    Purpose

    Expresses GFP-tagged PH-domain of SWAP70 in mammalian cells, impaired phosphoinositide binding

    Depositor

    Geert van den Bogaart

    Article

    Baranov et al Cell Rep. 2016 Nov 1;17(6):1518-1531. do

    Insert

    SWAP70 residues 209-306 with mutations R223E and R224E (SWAP70 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Pleckstrin-homology domain, residues 209-306, mut…

    Promoter

    CMV

    Available since

    Nov. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

18. pGY466

    Plasmid

    #166661

    Purpose

    For light-inducible Cre recombinase (LiCre) expression under the Met17 promoter in yeast

    Depositor

    Gaël Yvert

    Article

    Duplus-Bottin et al Elife. 2021 Feb 23;10. pii: 61268. doi:

    Insert

    LiCre
    

    Use

    Cre/Lox

    Tags

    Expression

    Yeast

    Mutation

    Cre: E340A, D341A

    Promoter

    MET17

    Available since

    April 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3.1-α-COPI-WD40

    Plasmid

    #180758

    Purpose

    Mammalian expression plasmid for Schizosaccharomyces pombe α-COPI-WD40 (residues 1-327)

    Depositor

    Syed Saif Hasan

    Article

    Dey et al Commun Biol. 2022 Feb 8;5(1):115. doi: 1

    Insert

    α-COPI-WD40
    

    Use

    Tags

    C-terminal strep tag

    Expression

    Mammalian

    Mutation

    Encodes residues 1-327; mutations: Leu181Lys, Leu…

    Promoter

    CMV

    Available since

    March 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pEGFP-C1-MKLP1 S708/710A

    Plasmid

    #70153

    Purpose

    mammalian expression of MKLP1 with an eGFP fusion and S708A/S710A mutations

    Depositor

    Masanori Mishima

    Article

    Douglas et al Curr Biol. 2010 May 25;20(10):927-33. do

    Insert

    MKLP1 (KIF23 Human)
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    S708A, S710A

    Promoter

    CMV

    Available since

    Nov. 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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