We narrowed to 11,122 results for: ARIA;

1. pCMV-T7-SpG-P2A-EGFP (RTW4177)

   Plasmid

   #139988

   Purpose

   CMV and T7 promoter expression plasmid for human codon optimized SpG(D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Article

   Walton et al Science. 2020 Apr 17;368(6488):290-296.

   Insert

   human codon optimized SpCas9 variant named SpG with BPNLS-3xFLAG-P2A-EGFP
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   BPNLS-3xFLAG-P2A-EGFP

   Expression

   Mammalian

   Mutation

   SpG=D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R

   Promoter

   CMV and T7

   Available since

   March 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pETM6-4F2-mCherry

   Plasmid

   #73421

   Purpose

   Reporter plasmid encoding mCherry, codon optimized for E. coli, transcriptionally driven by orthogonal T7-lac promoter variant 4F2.

   Depositor

   Mattheos Koffas

   Article

   Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw

   Insert

   Promoter 4F2 (orthogonal T7-lac variant)
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   P4F2 (orthogonal T7-lac variant)

   Available since

   March 1, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA-GFP-EphrinB3-Flag(A227Y)

   Plasmid

   #155013

   Purpose

   Expresses N-terminally GFP-tagged and C-terminally Flag-tagged EphrinB3 A227Y variant from pcDNA3.1

   Depositor

   Sinisa Urban

   Article

   Gandhi et al Cell Chem Biol. 2020 Aug 31. pii: S2451-

   Insert

   EphrinB3 (EPHB3 Human)
   

   Use

   Tags

   Flag, GFP, and signal peptide (residues 1 to 32) …

   Expression

   Mammalian

   Mutation

   A227Y

   Promoter

   CMV

   Available since

   Nov. 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pOTTC810 - pAAV-EF1a-CRTsigpep-GCaMP3 (D324G+D360G+397G+D435G 10.19)-KDEL

   Plasmid

   #63885

   Purpose

   An AAV packaging vector that expresses ER-retained low-affinity GCaMP3 variant under control of the EF1a promoter.

   Depositor

   Brandon Harvey

   Article

   Henderson et al PLoS One. 2015 Oct 9;10(10):e0139273. do

   Insert

   ER-localized low-affinity GCaMP3(10.19)
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   EF1a

   Available since

   Dec. 1, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. USPL1cat-C236S-pEYFP_C1

   Plasmid

   #85767

   Purpose

   mammalian expression of USPL1 catalytic domain (aa 212-514) as YFP fusion, catalytic inactive variant C236S

   Depositor

   Frauke Melchior

   Article

   Schulz et al EMBO Rep. 2012 Oct;13(10):930-8. doi: 10

   Insert

   USPL1, catalytic domain (aa 212-514), catalytic inactive variant C236S (USPL1 Human)
   

   Use

   Tags

   EYFP

   Expression

   Mammalian

   Mutation

   catalytic domain (aa 212-514), catalytic inactiv…

   Promoter

   CMV

   Available since

   Jan. 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pOTTC814 - pAAV-SYN1-CRTsigpep-GCaMP3 (D324G+D360G+397G+D435G 10.19)-KDEL

   Plasmid

   #63887

   Purpose

   An AAV packaging vector that expresses ER-retained low-affinity GCaMP3 variant under control of the SYN1 promoter.

   Depositor

   Brandon Harvey

   Article

   Henderson et al PLoS One. 2015 Oct 9;10(10):e0139273. do

   Insert

   ER-localized low-affinity GCaMP3(10.19)
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   human SYN1

   Available since

   Dec. 1, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pETM6-3F2-mCherry

   Plasmid

   #73418

   Purpose

   Reporter plasmid encoding mCherry, codon optimized for E. coli, transcriptionally driven by orthogonal T7-lac promoter variant 3F2.

   Depositor

   Mattheos Koffas

   Article

   Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw

   Insert

   Promoter 3F2 (orthogonal T7-lac variant)
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   P3F2 (orthogonal T7-lac variant)

   Available since

   March 1, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pETM6-3A2-mCherry

   Plasmid

   #73425

   Purpose

   Reporter plasmid encoding mCherry, codon optimized for E. coli, transcriptionally driven by orthogonal T7-lac promoter variant 3A2.

   Depositor

   Mattheos Koffas

   Article

   Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw

   Insert

   Promoter 3A2 (orthogonal T7-lac variant)
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   P3A2 (orthogonal T7-lac variant)

   Available since

   March 1, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pETM6-5F5-mCherry

   Plasmid

   #73422

   Purpose

   Reporter plasmid encoding mCherry, codon optimized for E. coli, transcriptionally driven by orthogonal T7-lac promoter variant 5F5.

   Depositor

   Mattheos Koffas

   Article

   Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw

   Insert

   Promoter 5F5 (orthogonal T7-lac variant)
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   P5F5 (orthogonal T7-lac variant)

   Available since

   March 14, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCKC775

    Plasmid

    #226626

    Purpose

    piggyBAC 7xTCF-LEF hCas9-T2A-A/T-biased2

    Depositor

    Chang Liu

    Article

    Carlson et al bioRxiv [Preprint]. 2024 Jun 11:2024.06.

    Inserts

    7xTCF-LEF, SpCas9, T2A, A/T-biased2 variant of TdT (DNTT Human, S. pyogenes)
    EF1a promoter, sfGFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    7xTCF-LEF and EF1a

    Available since

    Oct. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCKC764

    Plasmid

    #226625

    Purpose

    piggyBAC pCAG-hCas9-T2A-Unbiased3

    Depositor

    Chang Liu

    Article

    Carlson et al bioRxiv [Preprint]. 2024 Jun 11:2024.06.

    Inserts

    CAG promoter, SpCas9, T2A, Unbiased3 variant of TdT (DNTT Human, S. pyogenes, Chicken)
    EF1a promoter, blasticidin resistance
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and EF1a

    Available since

    Oct. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCKC776

    Plasmid

    #226627

    Purpose

    piggyBAC 4xHRE YB_TATA hCas9-T2A-G/C-biased1

    Depositor

    Chang Liu

    Article

    Carlson et al bioRxiv [Preprint]. 2024 Jun 11:2024.06.

    Inserts

    4x HRE_YB TATA, SpCas9, oxygen-dependent degron, T2A, G/C-biased1 variant of TdT (DNTT Human, S. pyogenes)
    EF1a promoter, mCherry
    

    Use

    Synthetic Biology

    Tags

    oxygen dependent degron (ODD)

    Expression

    Mammalian

    Mutation

    Promoter

    4xHRE_YB TATA and EF1a

    Available since

    Oct. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. Donor_N_FH_Piwi

    Plasmid

    #186651

    Purpose

    Donor plasmid to endogenously tag piwi gene in Drosophila melanogaster ovarian somatic sheet (OSS) cells.

    Depositor

    Astrid Haase

    Article

    Meng et al Nucleic Acids Res. 2022 May 27. pii: 659

    Insert

    OSS PIWI N-terminal 3xFlag-3xHA Tag Donor Plasmid (piwi Fly)
    

    Use

    Tags

    3xFlag-3xHA

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Aug. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA-GFP-EBA175-Flag(E1418K/A1419R/S1421R/P1424Q/Y1426S)

    Plasmid

    #155010

    Purpose

    Expresses N-terminally GFP-tagged and C-terminally Flag-tagged EBA175 E1418K/A1419R/S1421R/P1424Q/Y1426S variant (residues 1284-1462) from pcDNA3.1

    Depositor

    Sinisa Urban

    Article

    Gandhi et al Cell Chem Biol. 2020 Aug 31. pii: S2451-

    Insert

    PfEBA175
    

    Use

    Tags

    Flag, GFP, and signal peptide (residues 1 to 32) …

    Expression

    Mammalian

    Mutation

    E1418K/A1419R/S1421/P1424Q/Y1426S; insert codes f…

    Promoter

    CMV

    Available since

    Nov. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pETM6-1D4-mCherry

    Plasmid

    #73423

    Purpose

    Reporter plasmid encoding mCherry, codon optimized for E. coli, transcriptionally driven by orthogonal T7-lac promoter variant 1D4.

    Depositor

    Mattheos Koffas

    Article

    Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw

    Insert

    Promoter 1D4 (orthogonal T7-lac variant)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    P1D4 (orthogonal T7-lac variant)

    Available since

    March 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pETM6-4A6-mCherry

    Plasmid

    #73424

    Purpose

    Reporter plasmid encoding mCherry, codon optimized for E. coli, transcriptionally driven by orthogonal T7-lac promoter variant 4A6.

    Depositor

    Mattheos Koffas

    Article

    Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw

    Insert

    Promoter 4A6 (orthogonal T7-lac variant)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    P4A6 (orthogonal T7-lac variant)

    Available since

    March 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pETM6-1E4-mCherry

    Plasmid

    #73426

    Purpose

    Reporter plasmid encoding mCherry, codon optimized for E. coli, transcriptionally driven by orthogonal T7-lac promoter variant 1E4.

    Depositor

    Mattheos Koffas

    Article

    Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw

    Insert

    Promoter 1E4 (orthogonal T7-lac variant)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    P1E4 (orthogonal T7-lac variant)

    Available since

    March 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pETM6-1B6-mCherry

    Plasmid

    #73420

    Purpose

    Reporter plasmid encoding mCherry, codon optimized for E. coli, transcriptionally driven by orthogonal T7-lac promoter variant 1B6.

    Depositor

    Mattheos Koffas

    Article

    Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw

    Insert

    Promoter 1B6 (orthogonal T7-lac variant)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    P1B6 (orthogonal T7-lac variant)

    Available since

    March 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pETM6-3H5-mCherry

    Plasmid

    #73419

    Purpose

    Reporter plasmid encoding mCherry, codon optimized for E. coli, transcriptionally driven by orthogonal T7-lac promoter variant 3H5.

    Depositor

    Mattheos Koffas

    Article

    Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw

    Insert

    Promoter 3H5 (orthogonal T7-lac variant)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    P3H5 (orthogonal T7-lac variant)

    Available since

    March 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCAG-CBE4max-SpRY-P2A-EGFP (RTW5133)

    Plasmid

    #139999

    Purpose

    CAG promoter expression plasmid for human codon optimized BE4max C-to-T base editor with SpRY(D10A/A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1322R/R1333P/R1335Q/T1337R) and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Walton et al Science. 2020 Apr 17;368(6488):290-296.

    Insert

    human codon optimized CBE4max SpCas9 variant named SpRY with P2A-EGFP
    

    Use

    CRISPR

    Tags

    P2A-EGFP

    Expression

    Mammalian

    Mutation

    nSpCas9=D10A; SpRY=A61R/L1111R/D1135L/S1136W/G121…

    Promoter

    CAG

    Available since

    March 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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