We narrowed to 8,434 results for: Dos

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pET-Cas9-6xHis

Plasmid

#62374

Purpose

Expression of Cas9-6xHis in bacterial cells

Depositor

David Liu

Article

Zuris et al Nat Biotechnol. 2015 Jan;33(1):73-80. do

Insert

Cas9_6xHis

Tags

6xHis tag

Expression

Bacterial

Promoter

T7

Available Since

May 11, 2015

Availability

Academic Institutions and Nonprofits only

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pAAV.hSynap.(cyto).iGlucoSnFR2

Plasmid

#244091

Purpose

Cytosolic expression of green glucose sensor

Depositor

Tim Brown, HHMI-JRC Tool Translation Team

Article

Marvin et al Sci Adv. 2025 Nov 14;11(46):eadz3889. do

Insert

iGlucoSnFR2

Use

AAV

Available Since

Oct. 24, 2025

Availability

Academic Institutions and Nonprofits only

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mCherry-N2-Frankenbody-HA

Plasmid

#175292

Purpose

Visualize endogeneous loci coupled with ZF probes harboring 10xHA tag

Depositor

Tim Stasevich

Article

Liu et al Genes Cells. 2021 Nov;26(11):905-926. do

Insert

anti-HA frankenbody fused with mCherry

Tags

mCherry

Expression

Mammalian

Available Since

Oct. 7, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA3.1 TAP DHX36

Plasmid

#53547

Purpose

mammalian expression of DHX36

Depositor

Kathleen Collins

Article

Sexton et al Mol Cell Biol. 2011 Feb;31(4):736-43. do

Insert

DHX36 (DHX36 Human)

Tags

TAP

Expression

Mammalian

Promoter

CMV

Available Since

June 9, 2014

Availability

Academic Institutions and Nonprofits only

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pCDNA-FLAG-METTL3-APPA

Plasmid

#160251

Purpose

Express catalytic inactive FLAG-METTL3 in human cells

Depositor

Alessandro Fatica

Article

Sorci et al Cell Death Dis. 2018 Jul 23;9(8):796. do

Insert

methyltransferase like 3 (METTL3 Human)

Tags

FLAG

Expression

Mammalian

Mutation

changes in aa 395–398, DPPW → APPA

Promoter

CMV

Available Since

Nov. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pMOS003-lenti-CMV-GCaMPer

Plasmid

#65227

Purpose

ER calcium sensor for lentivirus production

Depositor

Adam Cohen, Brandon Harvey

Article

Henderson et al PLoS One. 2015 Oct 9;10(10):e0139273. do

Insert

GCaMPer

Use

Lentiviral

Tags

circularly permuted GFP

Expression

Mammalian

Mutation

GCaMP3 (D324G, D360G, D397G, D435G)

Promoter

CMV

Available Since

May 12, 2016

Availability

Academic Institutions and Nonprofits only

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pH1R-mCherry-N1

Plasmid

#84325

Purpose

Histamine-1 receptor tagged with mCherry

Depositor

Dorus Gadella

Article

van Unen et al Mol Pharmacol. 2016 Sep;90(3):162-76. do

Insert

H1R (HRH1 Human)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 12, 2016

Availability

Academic Institutions and Nonprofits only

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pGEX-5X-1-Full-Length-MePCE

Plasmid

#113539

Purpose

Produces an N-terminal GST-tagged full length MePCE protein.

Depositor

Blerta Xhemalce

Article

Shelton et al Cell Rep. 2018 Feb 6;22(6):1374-1383. do

Insert

MePCE (MEPCE Human)

Tags

GST

Expression

Bacterial

Available Since

July 26, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV-Frankenbody-FLAG-SNAP

Plasmid

#175295

Purpose

Track mature FLAG tagged proteins in live cells

Depositor

Tim Stasevich

Article

Liu et al Genes Cells. 2021 Nov;26(11):905-926. do

Insert

anti-FLAG frankenbody fused with SNAP-tag

Tags

SNAP-tag

Expression

Mammalian

Available Since

Oct. 19, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pET21-pelB-LaM-4

Plasmid

#172770

Purpose

Bacterial expression of anti-mCherry nanobody LaM-4, with pelB leader and C-term free cysteine and 6xHIS tag.

Depositor

Michael Rout

Article

Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

Insert

LaM-4

Tags

6xHIS and pelB leader for periplasmic secretion

Expression

Bacterial

Promoter

T7

Available Since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pETM33_IRF3_SMAD/FHA

Plasmid

#178479

Purpose

Bacterial expression of human domain with His-tag and GST tag

Depositor

Ylva Ivarsson

Article

Benz et al Mol Syst Biol. 2022 Jan;18(1):e10584. do

Insert

IRF3_IRF3 (IRF3 Synthetic, Human)

Tags

His-GST

Expression

Bacterial

Promoter

T7/LacO

Available Since

March 22, 2022

Availability

Academic Institutions and Nonprofits only

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pEYFP-N1-Pink1

Plasmid

#101874

Purpose

Transient expression of Pink1-YFP in mammalian cells

Depositor

Richard Youle

Article

Narendra et al PLoS Biol. 2010 Jan 26;8(1):e1000298. do

Insert

PTEN induced putative kinase 1 (PINK1 Human)

Tags

fluorescent tag EYFP

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 5, 2017

Availability

Academic Institutions and Nonprofits only

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PB-LacI-eGFP

Plasmid

#213021

Purpose

piggyBac donor vector containing LacI-eGFP for random integration by PBase

Depositor

Luca Giorgetti

Article

Mach et al Nat Genet. 2022 Dec;54(12):1907-1918. do

Insert

LacI**-eGFP

Use

Mouse Targeting; Piggybase integration (transposo…

Tags

eGFP

Expression

Mammalian

Available Since

April 10, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV/D374Y-hPCSK9

Plasmid

#58379

Purpose

Expresses GoF mutant human PCSK9 to be used for rAAV8-mediated gene transfer, hypercholesterolemia and atherosclerosis

Depositor

Jacob Bentzon

Article

Bjorklund et al Circ Res. 2014 May 23;114(11):1684-9. do

Insert

proprotein convertase subtilisin/kexin type 9 (PCSK9 Human)

Use

AAV

Expression

Mammalian

Mutation

D374Y

Promoter

HCRApoE/hAAT

Available Since

Aug. 26, 2014

Availability

Academic Institutions and Nonprofits only

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pLJC6-2XFLAG-TMEM192

Plasmid

#104435

Purpose

Expresses 2XFLAG-TMEM192 in mammalian cells

Depositor

David Sabatini

Article

Wyant et al Cell. 2017 Oct 19;171(3):642-654.e12. do

Insert

TMEM-192 (TMEM192 Human)

Use

Lentiviral

Tags

2XFLAG

Expression

Mammalian

Promoter

Ubiquitin

Available Since

July 2, 2018

Availability

Academic Institutions and Nonprofits only

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pJH3830

Plasmid

#200038

Purpose

Prig-3 zif-1 SL2 mCherry unc-54 3' UTR C.elegans AVA and other neurons expression of zif RFP

Depositor

Mei Zhen

Article

Meng et al Sci Adv. 2024 Apr 12;10(15):eadk0002. do

Insert

ZIF (zif-1 Nematode)

Tags

mCherry

Expression

Worm

Promoter

Prig-3

Available Since

May 7, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA-Cx43

Plasmid

#60691

Purpose

mammalian expression of rat Cx43

Depositor

Parmender Mehta

Article

Johnson et al Mol Biol Cell. 2013 Mar;24(6):715-33. do

Insert

Cx43 (Gja1 Rat)

Expression

Mammalian

Promoter

CMV

Available Since

July 6, 2015

Availability

Academic Institutions and Nonprofits only
TRE-TIGHT-EGFP-backward donor

Plasmid

#22077

Depositor

Rudolf Jaenisch

Article

Hockemeyer et al Nat Biotechnol. 2009 Sep;27(9):851-7. do

Insert

TETO-eGFP

Available Since

Oct. 5, 2009

Availability

Academic Institutions and Nonprofits only

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pCAGGS_NP_EBOV

Plasmid

#103049

Purpose

Encodes Ebola virus NP gene (Zaire ebolavirus, Mayinga) under control of the CAG promoter

Depositor

Elke Mühlberger

Article

Nelson et al Antiviral Res. 2017 Aug 12;146:21-27. do

Insert

NP gene of Ebola virus (NP Zaire ebolavirus, Mayinga)

Expression

Mammalian

Mutation

silent mutation (T to C) at position 928 of NP ge…

Promoter

CAG

Available Since

Dec. 20, 2017

Availability

Academic Institutions and Nonprofits only

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pDG462

Plasmid

#100903

Purpose

SpCas9n (D10A Nickase mutant) with 2A-Puro and a cloning backbone for 2 custom gRNAs which can be cloned in via a one-step reaction. For generation of DSBs with no off-target cuts.

Depositor

Paul Thomas

Article

Adikusuma et al PLoS One. 2017 Dec 6;12(12):e0187236. do

Inserts

humanized CRISPR associated protein 9 Nickase
U6-gRNA scaffold 1
U6-gRNA scaffold 2

Use

CRISPR and Mouse Targeting

Tags

3xFLAG and T2A-PuroR

Expression

Mammalian

Mutation

D10A mutant converts to Nickase

Promoter

CBh and U6

Available Since

Dec. 14, 2017

Availability

Academic Institutions and Nonprofits only

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