Sequence Type

BLAST Database

BLAST Algorithm

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

We narrowed to 8,983 results for: FIE

Showing: 4441 - 4460 of 8983 results

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pDONR223_PIK3CG_p.V168M

Plasmid

#81542

Purpose

Gateway Donor vector containing PIK3CG , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

PIK3CG (PIK3CG Human)

Use

Gateway Cloning

Mutation

E2G; V168M

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_PPIAL4G_WT

Plasmid

#81900

Purpose

Gateway Donor vector containing PPIAL4G , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

PPIAL4G (PPIAL4G Human)

Use

Gateway Cloning

Mutation

I6V; Q24L; H69R

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_PIK3IP1_p.G185E

Plasmid

#81339

Purpose

Gateway Donor vector containing PIK3IP1 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

PIK3IP1 (PIK3IP1 Human)

Use

Gateway Cloning

Mutation

G185E; T251S

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pET-47b-N-His-Ub2-I44A

Plasmid

#253393

Purpose

Expression in E. coli of linear di-ubiquitin carrying the I44A mutation.

Depositor

Kristijan Ramadan

Article

Song et al Nucleic Acids Res. 2025 Jul 8;53(13):gka

Insert

Ubiquitin (RPS27A Human)

Tags

His

Expression

Bacterial

Mutation

I44A

Available Since

May 27, 2026

Availability

Academic Institutions and Nonprofits only

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pET-47b-N-His-Ub2-I120A

Plasmid

#253394

Purpose

Expression in E. coli of linear di-ubiquitin carrying the I120A mutation.

Depositor

Kristijan Ramadan

Article

Song et al Nucleic Acids Res. 2025 Jul 8;53(13):gka

Insert

Ubiquitin (RPS27A Human)

Tags

His

Expression

Bacterial

Mutation

I120A

Available Since

May 27, 2026

Availability

Academic Institutions and Nonprofits only

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pET-47b-N-His-Ub2-I44AI120A

Plasmid

#253395

Purpose

Expression in E. coli of linear di-ubiquitin carrying the I44AI120A double mutation.

Depositor

Kristijan Ramadan

Article

Song et al Nucleic Acids Res. 2025 Jul 8;53(13):gka

Insert

Ubiquitin (RPS27A Human)

Tags

His

Expression

Bacterial

Mutation

I44AI120A

Available Since

May 27, 2026

Availability

Academic Institutions and Nonprofits only

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pET-24-hexaUb-I44AI120A

Plasmid

#253397

Purpose

Expression in E. coli of linear hexa-ubiquitin carrying the I44AI120A mutation, with His tag at C-terminus.

Depositor

Kristijan Ramadan

Article

Song et al Nucleic Acids Res. 2025 Jul 8;53(13):gka

Insert

Ubiquitin (RPS27A Human)

Tags

His

Expression

Bacterial

Mutation

I44AI120A

Available Since

May 27, 2026

Availability

Academic Institutions and Nonprofits only

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pCold I-Ub-mH1-Ncys (M1-C-T2)

Plasmid

#253410

Purpose

Expression in E. coli of a mouse H1 variant fused with His-tagged monoUb at N-terminus. An additional Cys was inserted between M1 and T2 in H1.

Depositor

Kristijan Ramadan

Article

Song et al Nucleic Acids Res. 2025 Jul 8;53(13):gka

Insert

H1.0 linker histone (H1f0 Mouse)

Tags

His

Expression

Bacterial

Mutation

M1-C-T2

Available Since

May 27, 2026

Availability

Academic Institutions and Nonprofits only

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pCold I-Ub4-mH1-Ncys (M1-C-T2)

Plasmid

#253411

Purpose

Expression in E. coli of a mouse H1 variant fused with His-tagged linear-tetraUb at N-terminus. An additional Cys was inserted between M1 and T2 in H1.

Depositor

Kristijan Ramadan

Article

Song et al Nucleic Acids Res. 2025 Jul 8;53(13):gka

Insert

H1.0 linker histone (H1f0 Mouse)

Tags

His

Expression

Bacterial

Mutation

M1-C-T2

Available Since

May 27, 2026

Availability

Academic Institutions and Nonprofits only

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pCAGGS-GFP-WTUBA7-IRES-mcherry

Plasmid

#246475

Purpose

Mammalian expression of AcGFP-tagged WT human UBA7, co-expresses mCherry; for transfection

Depositor

Francisco Bustos

Article

Bandi et al iScience. 2026 Mar 30;29(5):115454. doi:

Insert

AcGFP-WTUBA7-IRES-mcherry (UBA7 Human)

Tags

AcGFP1

Expression

Mammalian

Available Since

April 1, 2026

Availability

Academic Institutions and Nonprofits only

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pCAGGS-GFP-UBA7-V548L-IRES-mcherry

Plasmid

#246476

Purpose

Mammalian expression of AcGFP-tagged human UBA7-V548L, co-expresses mCherry; for transfection

Depositor

Francisco Bustos

Article

Bandi et al iScience. 2026 Mar 30;29(5):115454. doi:

Insert

AcGFP-UBA7-V548L-IRES-mcherry (UBA7 Human)

Tags

AcGFP1

Expression

Mammalian

Mutation

V548L

Available Since

April 1, 2026

Availability

Academic Institutions and Nonprofits only

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pCAGGS-GFP-UBA7-K709Sfs-IRES-mcherry

Plasmid

#246477

Purpose

Mammalian expression of AcGFP-tagged human UBA7-K709Sfs, co-expresses mCherry; for transfection

Depositor

Francisco Bustos

Article

Bandi et al iScience. 2026 Mar 30;29(5):115454. doi:

Insert

AcGFP-UBA7-K709S*fs-IRES-mcherry (UBA7 Human)

Tags

AcGFP1

Expression

Mammalian

Mutation

K709Sfs*

Available Since

April 1, 2026

Availability

Academic Institutions and Nonprofits only

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pTwist Amp High Copy FAM136A-HA mutant

Plasmid

#244882

Purpose

In vitro transcription of mutant human FAM136A downstream of SP6 promoter

Depositor

Alexis Jourdain

Article

Harhai et al Cell Rep. 2025 Jul 25;44(8):116069. doi:

Insert

FAM136A mutant (FAM136A Human)

Use

Synthetic Biology

Tags

HA

Expression

Bacterial

Mutation

GRCh38, chr2:70300842G>A

Promoter

SP6

Available Since

Oct. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pTwist Amp High Copy FAM136A-HA WT

Plasmid

#244883

Purpose

In vitro transcription of wild-type FAM136A downstream of SP6 promoter

Depositor

Alexis Jourdain

Article

Harhai et al Cell Rep. 2025 Jul 25;44(8):116069. doi:

Insert

FAM136A (FAM136A Human)

Use

Synthetic Biology

Tags

HA

Expression

Bacterial

Promoter

SP6

Available Since

Oct. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pTwist Amp High Copy ENDOG-HA

Plasmid

#244881

Purpose

In vitro transcription of human ENDOG downstream of SP6 promoter

Depositor

Alexis Jourdain

Article

Harhai et al Cell Rep. 2025 Jul 25;44(8):116069. doi:

Insert

ENDOG (ENDOG Human)

Use

Synthetic Biology

Tags

HA

Expression

Bacterial

Promoter

SP6

Available Since

Oct. 15, 2025

Availability

Academic Institutions and Nonprofits only

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pLV-FAM136A-WT-3xFLAG-hygro

Plasmid

#244880

Purpose

Overexpression of wild-type human FAM136A

Depositor

Alexis Jourdain

Article

Harhai et al Cell Rep. 2025 Jul 25;44(8):116069. doi:

Insert

FAM136A (FAM136A Human)

Use

Lentiviral

Tags

3xFLAG

Expression

Mammalian

Promoter

EF1a

Available Since

Oct. 15, 2025

Availability

Academic Institutions and Nonprofits only

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pLV-FAM136A-mutant-3xFLAG-hygro

Plasmid

#244879

Purpose

Overexpression of mutant human FAM136A

Depositor

Alexis Jourdain

Article

Harhai et al Cell Rep. 2025 Jul 25;44(8):116069. doi:

Insert

FAM136A mutant (FAM136A Human)

Use

Lentiviral

Tags

3xFLAG

Expression

Mammalian

Mutation

GRCh38, chr2:70300842G>A

Promoter

EF1a

Available Since

Oct. 15, 2025

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPR V2 puro HTRA2 sg2

Plasmid

#244864

Purpose

Knockout of human HTRA2

Depositor

Alexis Jourdain

Article

Harhai et al Cell Rep. 2025 Jul 25;44(8):116069. doi:

Insert

HTRA2 sgRNA (HTRA2 Human)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

Oct. 15, 2025

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPR V2 puro HTRA2 sg1

Plasmid

#244863

Purpose

Knockout of human HTRA2

Depositor

Alexis Jourdain

Article

Harhai et al Cell Rep. 2025 Jul 25;44(8):116069. doi:

Insert

HTRA2 sgRNA (HTRA2 Human)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

Oct. 15, 2025

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPR IMMP2L sg2

Plasmid

#244852

Purpose

Knockout of human IMMP2L

Depositor

Alexis Jourdain

Article

Harhai et al Cell Rep. 2025 Jul 25;44(8):116069. doi:

Insert

IMMP2L sgRNA (IMMP2L Human)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

Oct. 15, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge

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