We narrowed to 7,348 results for: Lif;

1. pLenti_dCas9-KRAB-MeCP2_Blast

   Plasmid

   #221501

   Purpose

   Lentiviral construct for expression of the dCas9-KRAB-MeCP2 CRISPRi vector

   Depositor

   Matthew Meyerson

   Article

   Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

   Insert

   dCas9-KRAB-MeCP2-T2A-Blast
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Cas9 mutations to make dCas9=D10A+D839A+H840A+N86…

   Promoter

   CMV

   Available since

   July 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. hsp70l-loxP-mCherry-STOP-loxP-H2B-GFP_cryaa-cerulean

   Plasmid

   #24334

   Depositor

   Didier Stainier

   Article

   Hesselson et al Proc Natl Acad Sci U S A. 2009 Sep 1. 10

   Insert

   H2B
   

   Use

   Cre/Lox

   Tags

   GFP

   Expression

   Worm

   Mutation

   mCherry driven by an HSP70 promoterCerulean drive…

   Promoter

   Available since

   April 27, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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3. pRCC-N

   Plasmid

   #81192

   Purpose

   Expression of Cas9 and gRNA cassette in S. cerevisiae; Nourseothricin Resistance

   Depositor

   Eckhard Boles

   Article

   Generoso et al J Microbiol Methods. 2016 Aug;127:203-5.

   Inserts

   Cas9
   empty gRNA cassette
   

   Use

   CRISPR

   Tags

   NTS

   Expression

   Yeast

   Mutation

   Please see depositor comments below. and WT - cod…

   Promoter

   ROX3 and SNP52p

   Available since

   Sept. 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA5/FRT/TO_H2B-HaloTag7-T2A-Tom20-HaloTag9

   Plasmid

   #175547

   Purpose

   Co-expression of HaloTag7 (nuclear) and 9 (mitochondrial surface) in mammalian cells

   Depositor

   Kai Johnsson

   Article

   Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

   Insert

   H2B-HaloTag7 and Tomm20-HaloTag9
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   HaloTag9 = HaloTag7-Q165H-P174R

   Promoter

   Available since

   Dec. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMA2640

   Plasmid

   #25434

   Purpose

   Retroviral Tet-On vector for CMV-driven rtTA with EGFP and Blasticidin selection

   Depositor

   Mikhail Alexeyev

   Article

   Alexeyev et al Mol Biol Rep. 2010 Apr . 37(4):1987-91.

   Insert

   reverse tetracycline-regulated transactivator Advanced
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 21, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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6. pPBbsr2-EKARrEV-NLS

   Plasmid

   #173855

   Purpose

   Encoding EKARrEV-NLS.

   Depositor

   Michiyuki Matsuda

   Article

   Lin et al Life Sci Alliance. 2021 Oct 19;5(1). pii

   Insert

   EKARrEV-NLS
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pScalps_Puro

   Plasmid

   #99636

   Purpose

   Empty backbone, 3rd generation lentiviral vector

   Depositor

   Silvia Monticelli

   Article

   Montagner et al Cell Rep. 2016 May 17;15(7):1566-79. doi

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   none

   Expression

   Mammalian

   Mutation

   Promoter

   SFFVp

   Available since

   Aug. 22, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCS2-mCherry-Rab11a

   Plasmid

   #184035

   Purpose

   Expresses mCherry Rab11a in mammalian cells and zebrafish embryos

   Depositor

   Heidi Hehnly

   Article

   Krishnan et al Life Sci Alliance. 2022 Mar 18;5(7). pii

   Insert

   Rab11a
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV IE94

   Available since

   May 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAtetO_6xHis-Bst-LF

   Plasmid

   #145799

   Purpose

   Plasmid for the aTc-inducible expression of the large-fragment of Bst DNAP

   Depositor

   Andrew Ellington

   Article

   Bhadra et al mSphere. 2021 May 19;6(3):e00911-20. doi

   Insert

   Bst-LF
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   tet PA

   Available since

   May 14, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. pLX-UBC_NKX2-1_Short_V5-GFP

    Plasmid

    #221499

    Purpose

    NKX2-1-GFP fusion protein lentiviral overexpression using an UBC promoter

    Depositor

    Matthew Meyerson

    Article

    Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

    Insert

    NKX2-1 (NKX2-1 Human)
    

    Use

    Lentiviral

    Tags

    V5-linker-GFP

    Expression

    Mammalian

    Mutation

    Promoter

    UBC

    Available since

    July 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. 3789 pmko-neo RNAi-GFP

    Plasmid

    #8881

    Depositor

    Stanley Korsmeyer

    Article

    Wang et al EMBO J. 2005 Jan 26. 24(2):368-81.

    Insert

    GFP
    

    Use

    RNAi and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 4, 2006

    Availability

    Academic Institutions and Nonprofits only

12. pWPI-TFDP1

    Plasmid

    #114299

    Purpose

    Constitutive expression of the TFDP1 protein

    Depositor

    Patrick Salmon

    Article

    Rady et al Cell Cycle. 2013 Aug 15;12(16):2691-702.

    Insert

    TFDP1 (TFDP1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBS SK mCherryROSAegfp

    Plasmid

    #54322

    Purpose

    Encodes mCherry fused with an out-of-frame EGFP through a linker containing a target for TALEN/CRISPR. Can be used as a surrogate target to test the specificity of TALENs and CRISPR.

    Depositor

    Silvestro Conticello

    Article

    Niccheri et al Cell Mol Life Sci. 2017 Apr 18. doi: 10.

    Insert

    mCherry and EGFP
    

    Use

    CRISPR, Mouse Targeting, and TALEN

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pDual-eGFP

    Plasmid

    #63215

    Purpose

    Expression of eGFP in bacteria and in mammalian cells. Used as a reporter for quantifying gene targeting and recombineering in mammalian cells.

    Depositor

    Richard S Myers

    Article

    Valledor et al IUBMB Life. 2012 Aug;64(8):684-9. doi: 1

    Insert

    His-T7-eGFP
    

    Use

    Lentiviral

    Tags

    His6 and T7

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    CMV-EF1α hybrid (CEF)

    Available since

    April 1, 2015

    Availability

    Academic Institutions and Nonprofits only

15. p210 pCMV-CREM

    Plasmid

    #8395

    Purpose

    Cre with chimeric/β-globin intron within the Cre coding sequence

    Depositor

    Jeffrey Green

    Article

    Kaczmarczyk et al Nucleic Acids Res 2001 Jun 15;29(12):E56

    Insert

    CREM
    

    Use

    Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    modified Cre

    Promoter

    CMV

    Available since

    July 18, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCSIIbsr-EKARrEV-NLS

    Plasmid

    #173854

    Purpose

    A lentiviral vector for EKARrEV-NLS

    Depositor

    Michiyuki Matsuda

    Article

    Lin et al Life Sci Alliance. 2021 Oct 19;5(1). pii

    Insert

    EKARrEV-NLS
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. GFP-Rabenosyn-5

    Plasmid

    #37538

    Depositor

    Silvia Corvera

    Article

    Navaroli et al Proc Natl Acad Sci U S A. 2012 Feb 21;10

    Insert

    Rabenosyn5 (RBSN Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 12, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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18. pRSETA-qmTQ2-citrate

    Plasmid

    #225251

    Purpose

    Bacterial expression of N-terminal His-tagged qmTQ2-citrate, a cyan fluorescent FLIM biosensor for detecting citrate

    Depositor

    Yasushi Okada

    Article

    Zhong et al Cell Rep Methods. 2024 Nov 18;4(11):1009

    Insert

    qmTQ2-citrate
    

    Use

    Tags

    6XHis, T7 tag, XpressTM tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    Sept. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pHEE901

    Plasmid

    #91707

    Purpose

    CRISPR/Cas9-mediated base editing in Arabidopsis. Contains Cas9(D10A) and empty gRNA scaffold, HygR

    Depositor

    Qi-Jun Chen

    Article

    Chen et al Sci China Life Sci. 2017 Mar 16. doi: 10

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    May 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCMV MDM-2

    Plasmid

    #16441

    Depositor

    Bert Vogelstein

    Article

    Oliner et al Nature. 1992 Jul 2. 358(6381):80-3.

    Insert

    MDM2 (MDM2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 11, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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