We narrowed to 15,575 results for: NOL

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pCMV_BE4max

Plasmid

#112093

Purpose

C:G-to-T:A base editing

Depositor

David Liu

Article

Koblan et al Nat Biotechnol. 2018 May 29. pii: nbt.41

Insert

BE4max

Use

CRISPR

Expression

Mammalian

Promoter

CMV

Available Since

June 26, 2018

Availability

Academic Institutions and Nonprofits only

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pComb3XSS

Plasmid

#63890

Purpose

3rd generation plasmid for phage display on modified geneIII, contains stuffer fragment

Depositor

Carlos Barbas

Article

Andris-Widhopf et al J Immunol Methods. 2000 Aug 28;242(1-2):

Inserts

SS Stuffer
Light Chain Stuffer
Heavy Chain Stuffer

Use

Phage display

Tags

6x His, HA tag, and geneIII

Expression

Bacterial

Promoter

lacZ

Available Since

July 21, 2015

Availability

Academic Institutions and Nonprofits only

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circRNA-synIRES-R25-mNeonGreen

Plasmid

#188115

Purpose

Template plasmid to transcribe circular RNA encoding mNeonGreen

Depositor

Howard Chang

Article

Chen et al Nat Biotechnol. 2022 Jul 18. pii: 10.103

Insert

mNeonGreen

Expression

Mammalian

Promoter

T7

Available Since

Oct. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pRC0940 (circRNA entry vector)

Plasmid

#189967

Purpose

Cloning vector for generating template plasmids to transcribe circRNA, with mNeonGreen dropout following BsaI digestion

Depositor

Howard Chang

Article

Chen et al Nat Biotechnol. 2022 Jul 18. pii: 10.103

Insert

mNeonGreen

Expression

Mammalian

Promoter

T7

Available Since

Oct. 17, 2022

Availability

Academic Institutions and Nonprofits only

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mCherry-pBAD

Plasmid

#54630

Purpose

Localization: Protein Expression Vector , Excitation: 587, Emission: 610

Depositor

Michael Davidson, Nathan Shaner, Roger Tsien

Article

Shaner et al Nat Biotechnol. 2004 Dec;22(12):1567-72.

Type

Empty backbone

Tags

6xHis and mCherry

Expression

Bacterial

Available Since

July 25, 2014

Availability

Academic Institutions and Nonprofits only

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AbVec2.0-IGHG1

Plasmid

#80795

Purpose

Expression of secretory immunoglobulin heavy chains in mammalian cells, human IgG1 isotype

Depositor

Hedda Wardemann

Article

Tiller et al J Immunol Methods. 2008 Jan 1;329(1-2):1

Type

Empty backbone

Expression

Mammalian

Promoter

hCMV IE1 gene promoter

Available Since

June 28, 2017

Availability

Academic Institutions and Nonprofits only

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pCAG-iCre

Plasmid

#89573

Purpose

Expresses iCre under pCAG

Depositor

Wilson Wong

Article

Weinberg et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

Insert

iCre

Use

Cre/Lox and Synthetic Biology

Tags

NLS

Expression

Mammalian

Available Since

April 27, 2017

Availability

Academic Institutions and Nonprofits only

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pRC0569 (parts entry vector)

Plasmid

#189966

Purpose

Cloning vector for generating part plasmids, with mNeonGreen dropout following BsmBI digestion

Depositor

Howard Chang

Article

Chen et al Nat Biotechnol. 2022 Jul 18. pii: 10.103

Insert

mNeonGreen

Expression

Mammalian

Promoter

BBa-J23100

Available Since

Sept. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3-10His-mStayGold (E138D)

Plasmid

#211363

Purpose

Mammalian expression of the bright and photostable monomeric StayGold fluorescent protein (E138D). Contains a N-terminal 10xHis tag.

Depositor

Mohan Balasubramanian

Article

Ivorra-Molla et al Nat Biotechnol. 2023 Dec 11. doi: 10.103

Insert

mStayGold

Tags

10xHis-tag

Expression

Mammalian

Mutation

E138D

Promoter

CMV

Available Since

Dec. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pBAD-HisD-miniGFP1

Plasmid

#188896

Purpose

Express 6xHis-Tag miniGFP1 in E.coli

Depositor

Kiryl Piatkevich

Article

Liang et al Front Bioeng Biotechnol. 2022 Oct 17;10:

Insert

miniGFP1

Tags

6xHis

Expression

Bacterial

Promoter

araBAD

Available Since

Feb. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pGFPGUSPlus

Plasmid

#64401

Purpose

Binary plant vector expressing GFP and GUS, both driven by a 35S promoter

Depositor

Claudia Vickers

Article

Vickers et al Biotechnol Lett. 2007 Nov;29(11):1793-6.

Inserts

GFP
GUSPlus

Use

Binary vector for plant transformation

Tags

His

Expression

Plant

Mutation

S65T

Promoter

35S

Available Since

May 19, 2015

Availability

Academic Institutions and Nonprofits only

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pET29b-IPP1-His

Plasmid

#124137

Purpose

Bacterial expression plasmid for PURE system components

Depositor

Sebastian Maerkl, Takuya Ueda

Article

Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

Insert

Inorganic pyrophosphatase (IPP1 Budding Yeast)

Tags

6x-His

Expression

Bacterial

Available Since

April 19, 2019

Availability

Academic Institutions and Nonprofits only

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pBAMD1-4

Plasmid

#61565

Purpose

Standardized mini-Tn5 delivery plasmid, streptomycin/spectinomycin resistant

Depositor

Víctor de Lorenzo

Article

Martinez-Garcia et al Front Bioeng Biotechnol. 2014 Oct 28;2:4

Type

Empty backbone

Use

Synthetic Biology; Mini-tn5 vector

Available Since

Jan. 7, 2015

Availability

Academic Institutions and Nonprofits only

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pBAD-HisD-miniGFP2

Plasmid

#188897

Purpose

Express 6xHis-Tag miniGFP2 in E.coli

Depositor

Kiryl Piatkevich

Article

Liang et al Front Bioeng Biotechnol. 2022 Oct 17;10:

Insert

miniGFP2

Tags

6xHis

Expression

Bacterial

Promoter

araBAD

Available Since

Feb. 21, 2023

Availability

Academic Institutions and Nonprofits only

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PCS2+Cas9-mSA

Plasmid

#103882

Purpose

Cas9 mSA for in vitro transcription for gene editing in Mouse Embryos

Depositor

Janet Rossant

Article

Gu et al Nat Biotechnol. 2018 Jun 11. pii: nbt.41

Insert

CAS9-MSA

Use

CRISPR

Tags

MSA (monomeric streptavidin

Mutation

none

Promoter

SP6

Available Since

Nov. 30, 2017

Availability

Academic Institutions and Nonprofits only

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19ag-SOD2-3xHA-TALE(RNR2-Left)-MutH

Plasmid

#209294

Purpose

mitoBE expression vector, target mtRNR2 gene.

Depositor

Wensheng Wei

Article

Yi et al Nat Biotechnol. 2023 May 22. doi: 10.103

Insert

SOD2-3xHA-TALE(RNR2-Left)-MutH

Expression

Mammalian

Available Since

Nov. 16, 2023

Availability

Academic Institutions and Nonprofits only

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pTK299

Plasmid

#59568

Purpose

SIN SP6 P1234 (nsP2 P726S) SGP(14) Kozak L7Ae-P2A-EYFP 4xFF4

Depositor

Ron Weiss

Article

Wroblewska et al Nat Biotechnol. 2015 Aug 3. doi: 10.1038

Insert

SGP(14) Kozak L7Ae-P2A-EYFP 4xFF4

Available Since

Aug. 3, 2015

Availability

Academic Institutions and Nonprofits only

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pTol2-hspDRv7_scGstlt

Plasmid

#108870

Purpose

scGESTALT V7 lineage barcode for zebrafish

Depositor

Alex Schier

Article

Raj et al Nat Biotechnol. 2018 Mar 28. pii: nbt.41

Insert

scGESTALT lineage tracing target V7

Use

Tol2 integration into zebrafish genome

Available Since

April 25, 2018

Availability

Academic Institutions and Nonprofits only

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ePE6d

Plasmid

#229029

Purpose

Expresses ePE6d — engineered SpCas9 nickase (nCas9), RNA chaperone nucleocapsid (NC) protein, with T128N/N200C/V223Y variant of MMLVΔRNaseH — for gene editing in rice

Depositor

Li Juan

Article

Xu et al Plant Biotechnol J. 2024 Sep 27. doi: 10

Insert

Cas9

Use

CRISPR

Available Since

Feb. 28, 2025

Availability

Academic Institutions and Nonprofits only

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22l-COX8-3xFlag-TALE(RNR2-Right)-TadA(8e+V106W)

Plasmid

#209296

Purpose

mitoBE expression vector, target mtRNR2 gene.

Depositor

Wensheng Wei

Article

Yi et al Nat Biotechnol. 2023 May 22. doi: 10.103

Insert

COX8-3xFlag-TALE(RNR2-Right)-TadA(8e+V106W)

Expression

Mammalian

Available Since

Nov. 16, 2023

Availability

Academic Institutions and Nonprofits only

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