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pAPM-miR30-LRPPRC-ts1

Plasmid

#174268

Purpose

LRPPRC knockdown

Depositor

Jeremy Luban

Article

Guney et al Proc Natl Acad Sci U S A. 2024 Jul 16;12

Insert

LRPPRC shRNA (LRPPRC Human)

Use

Lentiviral and RNAi

Promoter

SFFV

Available Since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pAPM-miR30-LRPPRC-ts2

Plasmid

#174269

Purpose

LRPPRC knockdown

Depositor

Jeremy Luban

Article

Guney et al Proc Natl Acad Sci U S A. 2024 Jul 16;12

Insert

LRPPRC shRNA (LRPPRC Human)

Use

Lentiviral and RNAi

Promoter

SFFV

Available Since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pFRT/FLAG/HA-DEST EIF2C1

Plasmid

#19887

Depositor

Thomas Tuschl

Article

Landthaler et al RNA. 2008 Dec;14(12):2580-96. doi: 10.12

Insert

Eukaryotic translation initiation factor 2C, 1 (AGO1 Human)

Tags

FLAG/HA

Expression

Mammalian

Available Since

Dec. 31, 2008

Availability

Academic Institutions and Nonprofits only

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pEGB3 alpha1 P35S:PhiC31 integrase-RDF:T35S (GB2893)

Plasmid

#160633

Purpose

TU for the constitutive expression of the PhiC31 integrase, C-terminally fused with its reversion factor (RDF)

Depositor

Diego Orzaez

Article

Bernabe-Orts et al Nucleic Acids Res. 2020 Apr 6;48(6):3379

Insert

PhiC31-RDF

Use

Synthetic Biology

Expression

Plant

Mutation

BsaI and BsmBI sites removed

Promoter

35S

Available Since

March 29, 2021

Availability

Academic Institutions and Nonprofits only

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pFRT/TO/HIS/FLAG/HA-EDF1

Plasmid

#38079

Depositor

Markus Landthaler

Article

Baltz et al Mol Cell. 2012 Jun 8;46(5):674-90.

Insert

EDF1 endothelial differentiation-related factor 1 (EDF1 Human)

Tags

HIS/FLAG/HA

Expression

Mammalian

Available Since

Sept. 17, 2012

Availability

Academic Institutions and Nonprofits only

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pCCLc-U6-shHMGA2.3-PGK-dTomato

Plasmid

#89606

Purpose

Silencing Hmga2 in human cells

Depositor

Fernando Fierro

Article

Kalomoiris et al J Cell Biochem. 2016 Sep;117(9):2128-37.

Insert

shRNA targeting HMGA2 (HMGA2 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

July 20, 2017

Availability

Academic Institutions and Nonprofits only

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ACVR1-TAAS-R206H

Plasmid

#206184

Purpose

in vitro transcription of RNA encoding human ACVR1 with mutations S190A, S192A, and R206H

Depositor

Mary Mullins

Article

Allen et al J Bone Miner Res. 2023 Jun 17. doi: 10.1

Insert

activin A receptor type 1 (ACVR1 Human)

Use

In vitro transcription

Tags

FLAG tag

Mutation

S190A, S192A, R206H

Available Since

Oct. 19, 2023

Availability

Academic Institutions and Nonprofits only

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pBJG1 T7-8xHis-OGT D554N

Plasmid

#154279

Purpose

Bacterial expression of full length human OGT with D554N mutation (Cleavage, no Ser/Thr glycosylation). N-terminal T7_leader-8xHis tag followed by HRV3C protease site.

Depositor

Suzanne Walker

Article

Janetzko et al Nat Chem Biol. 2016 Nov;12(11):899-901.

Insert

O-GlcNAc Transferase (OGT Human)

Tags

8x His, HRV3C protease site, and T7 leader

Expression

Bacterial

Mutation

Aspartate 554 changed to Asparagine- bacterial co…

Available Since

Sept. 22, 2020

Availability

Academic Institutions and Nonprofits only

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pCCLc-MNDU3-LIN28A-PGK-EGFP-WPRE

Plasmid

#89607

Purpose

Expression of LIN28A in human cells

Depositor

Fernando Fierro

Article

Kalomoiris et al J Cell Biochem. 2016 Sep;117(9):2128-37.

Insert

LIN28A (LIN28A Human)

Use

Lentiviral

Expression

Mammalian

Promoter

MNDU3

Available Since

July 20, 2017

Availability

Academic Institutions and Nonprofits only

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pFRT/TO/HIS/FLAG/HA-CSNK1E

Plasmid

#38078

Depositor

Markus Landthaler

Article

Baltz et al Mol Cell. 2012 Jun 8;46(5):674-90.

Insert

CSNK1E casein kinase 1, epsilon (CSNK1E Human)

Tags

HIS/FLAG/HA

Expression

Mammalian

Available Since

Sept. 17, 2012

Availability

Academic Institutions and Nonprofits only

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pCMV Flag dPDZ-PTEN (1-400)

Plasmid

#22232

Depositor

Hong Wu

Article

Mosessian et al J Biol Chem. 2009 Oct 30;284(44):30159-6

Insert

PTEN (Pten Mouse)

Tags

Flag

Expression

Bacterial and Mammalian

Mutation

PDZ binding domain deleted. Contains amino acids …

Available Since

March 19, 2010

Availability

Academic Institutions and Nonprofits only
pAPM-miR30-EIF2AK2-ts1

Plasmid

#174247

Purpose

EIF2AK2 knockdown

Depositor

Jeremy Luban

Article

Guney et al Proc Natl Acad Sci U S A. 2024 Jul 16;12

Insert

EIF2AK2 shRNA (EIF2AK2 Human)

Use

Lentiviral and RNAi

Promoter

SFFV

Available Since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

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SBC009876

Plasmid

#226283

Purpose

Expresses PhCFAT and ObEGS1 from trc promoter. Biosynthesis of phenylpropenes from (hydroxy)cinnamyl alcohols.

Depositor

Nigel Scrutton

Article

Robinson et al Metab Eng. 2020 Jul;60:168-182. doi: 10.

Inserts

Coniferyl alcohol acyltransferase
Eugenol synthase 1

Expression

Bacterial

Available Since

Oct. 17, 2024

Availability

Academic Institutions and Nonprofits only

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pTIGER_mNeonGreen::3xFLAG::dCrk

Plasmid

#131137

Purpose

UASp construct for over-expression of Drosophila Crk with N-terminal monomeric NeonGreen and 3X FLAG tag. Compatible with PhiC31-mediated site-specific integration or classical P-element insertion.

Depositor

Mark Peifer

Article

Spracklen et al Mol Biol Cell. 2019 Jul 18:mbcE19050302.

Insert

Crk (Crk Fly)

Tags

3XFLAG and mNeonGreen

Expression

Insect

Available Since

Oct. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pCCLc-U6-shHMGA2.2-PGK-dTomato

Plasmid

#89605

Purpose

Silencing Hmga2 in human cells

Depositor

Fernando Fierro

Article

Kalomoiris et al J Cell Biochem. 2016 Sep;117(9):2128-37.

Insert

shRNA targeting HMGA2 (HMGA2 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

July 21, 2017

Availability

Academic Institutions and Nonprofits only

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pEGFP-C3-PLK4-K41M-S285A-T289A-3xFLAG

Plasmid

#69842

Purpose

This plasmid encodes kinase dead PLK4 isoform 1 carrying K41M and S285A/T289A mutations with an N-terminal EGFP tag and C-terminal 3xFLAG tag

Depositor

Michel Bornens

Article

Sillibourne et al Mol Biol Cell. 2010 Feb 15;21(4):547-61.

Insert

PLK4 (PLK4 Human)

Tags

3xFLAG tag and EGFP

Expression

Mammalian

Mutation

K41M-S285A-T289A

Promoter

CMV

Available Since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only

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Frt-hspB8

Plasmid

#63099

Purpose

Expression of HSPB8 (small HSP) in mammalian cells

Depositor

Harm Kampinga

Article

Vos et al Hum Mol Genet. 2010 Dec 1;19(23):4677-93

Insert

hspb8 (HSPB8 Human)

Expression

Mammalian

Promoter

CMV

Available Since

March 18, 2015

Availability

Academic Institutions and Nonprofits only

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sgAAVS1(A)

Plasmid

#85570

Purpose

Expresses sgRNA targeting human AAVS1 locus

Depositor

Luca Grumolato

Article

Guernet et al Mol Cell. 2016 Aug 4;63(3):526-38. doi:

Insert

adeno-associated virus integration site 1 (AAVS1 Human)

Expression

Mammalian

Available Since

Feb. 8, 2017

Availability

Academic Institutions and Nonprofits only
pENTR-2B-Gata4

Plasmid

#98615

Purpose

Gateway entry vector for Gata4

Depositor

Richard Harvey

Article

Bouveret et al Elife. 2015 Jul 6;4. doi: 10.7554/eLife.

Insert

Gata4 (Gata4 Mouse)

Use

Entry vector

Available Since

July 26, 2017

Availability

Academic Institutions and Nonprofits only

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LentiGuide-Puro-NCOA7g1 (BB22)

Plasmid

#139457

Purpose

Lentiviral vector with gRNA targeting human NCOA7 short isoform; includes puromycin selectable marker

Depositor

Caroline Goujon

Article

Doyle et al Nat Microbiol. 2018 Dec;3(12):1369-1376.

Insert

NCOA7 short isoform-targeting sgRNA inserted; resistance gene: puroR (NCOA7 Synthetic)

Use

Lentiviral

Expression

Mammalian

Promoter

EF-1a / U6

Available Since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

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