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pLenti-SCGB3A2 promoter-EGFP-EF1a-TagRFP

Plasmid

#204821

Purpose

Lentiviral vector for human SCGB3A2 promoter-driven expression of EGFP

Depositor

Emma Rawlins

Article

He et al Cell. 2022 Dec 8;185(25):4841-4860.e25.

Insert

An upstream region including human SCGB3A2 promoter

Use

Lentiviral

Expression

Mammalian

Promoter

SCGB3A2 promoter

Available Since

Aug. 7, 2023

Availability

Academic Institutions and Nonprofits only

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Ai62(TITL-tdT) Flp-in replacement vector

Plasmid

#61576

Purpose

Recombinase-mediated cassette exchange in ES cells to insert a Cre & tTA-dependent tdTomato expression cassette into a docking site within the mouse TIGRE genomic locus

Depositor

Hongkui Zeng

Article

Madisen et al Neuron. 2015 Mar 4;85(5):942-58. doi: 10

Insert

chromatin insulator flanked TRE-LSL-tdTomato

Use

Recombinase-mediated cassette exchange using flp …

Available Since

March 18, 2015

Availability

Academic Institutions and Nonprofits only

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pDONR223_TNFRSF17_WT

Plasmid

#82263

Purpose

Gateway Donor vector containing TNFRSF17 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

TNFRSF17 (TNFRSF17 Human)

Use

Gateway entry vector

Mutation

N81S; K86E

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_PPARG_WT

Plasmid

#82223

Purpose

Gateway Donor vector containing PPARG , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

PPARG (PPARG Human)

Use

Gateway entry vector

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_PRKCA_WT

Plasmid

#82225

Purpose

Gateway Donor vector containing PRKCA , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

PRKCA (PRKCA Human)

Use

Gateway entry vector

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pOTTC1181 - pmU6(loxP-STOP-loxP) BbsI gRNA

Plasmid

#113160

Purpose

A plasmid for cloning Cre-dependent guide RNAs using a modified mouse U6 promoter containing loxP

Depositor

Brandon Harvey

Article

Back et al Neuron. 2019 Feb 8. pii: S0896-6273(19)3

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Promoter

mU6

Available Since

Feb. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pDONR223_TBK1_WT

Plasmid

#82285

Purpose

Gateway Donor vector containing TBK1, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

TBK1 (TBK1 Human)

Use

Gateway entry vector

Mutation

N388D; K570Q

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_MLST8_WT

Plasmid

#82258

Purpose

Gateway Donor vector containing MLST8 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

MLST8 (MLST8 Human)

Use

Gateway entry vector

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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TMEM216-mCherry

Plasmid

#41633

Depositor

Joseph Gleeson

Article

Lee et al Science. 2012 Feb 24;335(6071):966-9. Ep

Insert

Transmembrane Protein 216 (TMEM216 Human)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

April 24, 2013

Availability

Academic Institutions and Nonprofits only
pDONR223_PIK3CB_WT

Plasmid

#82221

Purpose

Gateway Donor vector containing PIK3CB , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

PIK3CB (PIK3CB Human)

Use

Gateway entry vector

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_SOCS3_WT

Plasmid

#82245

Purpose

Gateway Donor vector containing SOCS3 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

SOCS3 (SOCS3 Human)

Use

Gateway entry vector

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pOTTC1080 - prRosa26v2 LSL FLAG-SpCas9

Plasmid

#113163

Purpose

A donor plasmid with homologous arms matching rat Rosa26 and expressing a Cre-dependent FLAG-tagged SpCas9

Depositor

Brandon Harvey

Article

Back et al Neuron. 2019 Feb 8. pii: S0896-6273(19)3

Insert

SpCas9

Use

CRISPR

Tags

FLAG

Expression

Mammalian

Promoter

CAG

Available Since

March 28, 2019

Availability

Academic Institutions and Nonprofits only

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Slc17a7-IRES2-Cre targeting vector

Plasmid

#61574

Purpose

Target the Cre recombinase gene to the stop codon of the mouse Slc17a7 (VGlut1) gene

Depositor

Hongkui Zeng

Article

Madisen et al Neuron. 2015 Mar 4;85(5):942-58. doi: 10

Insert

Slc17a7-IRES2-Cre

Use

Cre/Lox and Mouse Targeting

Available Since

July 30, 2015

Availability

Academic Institutions and Nonprofits only

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MSCV-ASNS-OE-IRES-Thy1.1

Plasmid

#192947

Purpose

Asparagine synthetase overexpression in murine cells

Depositor

Ping-Chih Ho

Article

Fernandez-Garcia et al Cell Rep. 2022 Nov 15;41(7):111639. doi:

Insert

Asparagine synthetase (Asns Mouse)

Use

Retroviral

Expression

Mammalian

Promoter

5'LTR

Available Since

Jan. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pDONR223_RBM10_WT

Plasmid

#81958

Purpose

Gateway Donor vector containing RBM10, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

RBM10 (RBM10 Human)

Use

Gateway entry vector

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_IRGM_WT

Plasmid

#82134

Purpose

Gateway Donor vector containing IRGM , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

IRGM (IRGM Human)

Use

Gateway entry vector

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pMMS22L-siR3

Plasmid

#221013

Purpose

mammalian expression vector of myc-Flag tagged MMS22L siRNA resistant

Depositor

Daniel Durocher

Article

O'Donnell et al Mol Cell. 2010 Nov 24;40(4):619-31. doi:

Insert

MMS22L (MMS22L Human)

Tags

Flag and myc

Expression

Mammalian

Mutation

silent mutations MMS22L: 5'-aaAacGtgTtgCtgTg…

Promoter

CMV

Available Since

July 31, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pDONR223_RIMS2_WT

Plasmid

#81803

Purpose

Gateway Donor vector containing RIMS2 ,part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

RIMS2 (RIMS2 Human)

Use

Gateway entry vector

Mutation

S86F; Q295R

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_SMURF2_WT

Plasmid

#82259

Purpose

Gateway Donor vector containing SMURF2 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

SMURF2 (SMURF2 Human)

Use

Gateway entry vector

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_RHEB_WT

Plasmid

#81257

Purpose

Gateway Donor vector containing RHEB , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

RHEB (RHEB Human)

Use

Gateway entry vector

Mutation

WT

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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