We narrowed to 7,208 results for: ef1a;

1. pAAV-DIO-dAPEX2

   Plasmid

   #117174

   Purpose

   Peroxidase reporter for multiplexed electron microscopy labeling

   Depositor

   David Ginty

   Article

   Zhang et al Nat Neurosci. 2019 Mar 18. pii: 10.1038/

   Insert

   dAPEX2
   

   Use

   AAV and Cre/Lox

   Tags

   Expression

   Mammalian

   Mutation

   W41F and A134P on soybean APX

   Promoter

   EF1a

   Available Since

   March 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. Lenti_MCP-LSD1_Hygro

   Plasmid

   #138457

   Purpose

   lenti vector encoding the MS2-LSD1 with a 2A Hygro resistance marker

   Depositor

   Jian Xu

   Article

   Li et al Nat Commun. 2020 Jan 24;11(1):485. doi:

   Insert

   LSD1
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available Since

   May 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pSBbi_RP_lbMicroID2-HA

   Plasmid

   #188522

   Purpose

   Encodes the low background biotin proximity labeling enzyme lbMicroID2 for expression and stable integration into mammalian cells

   Depositor

   James Londino

   Article

   Johnson et al Mol Cell Proteomics. 2022 Jun 7:100256.

   Insert

   lbMicroID2
   

   Use

   Transposon mediated gene integration

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a/RPBSA

   Available Since

   Sept. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. SaCas9

   Plasmid

   #188494

   Purpose

   Expresses FLAG tagged SaCas9

   Depositor

   Isaac Hilton

   Article

   Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

   Insert

   Cas9
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available Since

   Sept. 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pRDA_118

   Plasmid

   #133459

   Purpose

   U6 promoter expresses customizable Spyo-guide; EF1a promoter provides puromycin resistance. This vector is a derivative of the lentiGuide vector, with minor modifications to the tracrR

   Depositor

   John Doench

   Article

   DeWeirdt et al Nat Commun. 2020 Feb 6;11(1):752. doi: 1

   Insert

   control guide
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Oct. 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. PB-TO-NGN2-EMX1 BSD-mApple

   Plasmid

   #182311

   Purpose

   Piggybac Tet-ON plasmid for differentiating hiPSCs into glutamatergic neurons via NGN2 and EMX1 expression, bsd selection, mApple

   Depositor

   iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward

   Inserts

   hNGN2-P2A-EMX1 (NEUROG2, EMX1 Human)
   bsd-T2A-mycNLS-mApple
   

   Use

   Tags

   T2A-mycNLS-mApple

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a and TRE3G

   Available Since

   April 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. AsCas12a

   Plasmid

   #188492

   Purpose

   Expresses FLAG tagged AsCas12a

   Depositor

   Isaac Hilton

   Article

   Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

   Insert

   Cas12a
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available Since

   Sept. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pLenti-FoxO1-Clover

   Plasmid

   #67759

   Purpose

   Fluorescent fusion protein for tracking Akt signaling

   Depositor

   Peter Rotwein

   Article

   Gross et al J Cell Sci. 2015 Jun 3. pii: jcs.168773.

   Insert

   Fox01 (Foxo1 Mouse)
   

   Use

   Lentiviral

   Tags

   Clover

   Expression

   Mutation

   Changed Serine 209 to Alanine, changed Histidine …

   Promoter

   EF1a

   Available Since

   Aug. 12, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. SpCas9

   Plasmid

   #188489

   Purpose

   Expresses FLAG tagged SpCas9

   Depositor

   Isaac Hilton

   Article

   Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

   Insert

   Cas9
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available Since

   Sept. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pDD428

    Plasmid

    #202081

    Purpose

    Cas9/sgRNA plasmid targeting the C-terminus of Myh9

    Depositor

    Daniel Dickinson

    Article

    Shi et al Development. 2023 May 15;150(10):dev2013

    Insert

    pEF1a-mCherry-Cas9 + Myh9 CT sgRNA (Myh9 S. pyogenes (Cas9))
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available Since

    July 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge