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tdminiSOG-PCNA-19

Plasmid

#57806

Purpose

Localization: Nucleus, Excitation: 448 / 473, Emission: 500 / 528

Depositor

Michael Davidson

Publication

Shu et al PLoS Biol. 2011 Apr;9(4):e1001041. doi:

Insert

PCNA (PCNA Human)

Tags

tdminiSOG

Expression

Mammalian

Promoter

CMV

Available since

Jan. 29, 2015

Availability

Industry, Academic Institutions, and Nonprofits
ReaLiSM A1-Hinge-GPIba+2cys

Plasmid

#59581

Purpose

express VWF A1-GPIba ReaLiSM construct in mammalian cells

Depositor

Timothy Springer

Publication

Kim et al Nature. 2010 Aug 19;466(7309):992-5. doi

Insert

VWF A1-linker-GPIba (VWF Human)

Tags

6His

Expression

Mammalian

Promoter

chick beta-actin

Available since

Oct. 31, 2014

Availability

Academic Institutions and Nonprofits only
L4671

Plasmid

#1670

Depositor

Andrew Fire

Type

Empty backbone

Tags

myo3-2341, myo3Tsa, SV40NLS, gfpN, IVSR, gfpGLA, …

Expression

Worm

Available since

March 7, 2005

Availability

Academic Institutions and Nonprofits only

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L2685

Plasmid

#1520

Depositor

Andrew Fire

Type

Empty backbone

Tags

hsp16-25', hsp16-415', IVSA, SV40NLS, g…

Expression

Worm

Available since

March 7, 2005

Availability

Academic Institutions and Nonprofits only

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YW347_Ptbb2UTR-loxP-RFP-miniMOS

Plasmid

#196064

Purpose

a plasmid modified from miniMOS vector pCFJ910. the modification including insertions of myo2p::TagRFP downstream to NeoR CDS and 2 loxP sites flanking the NeoR and tagRFP cassettes.

Depositor

Wenxing Yang

Publication

Li et al PeerJ. 2023 May 17;11:e15433. doi: 10.77

Inserts

myo2p driven tagRFP
NeoR
Minimal Mos1

Expression

Worm

Promoter

myo-2

Available since

Jan. 31, 2023

Availability

Academic Institutions and Nonprofits only

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pCFJ2301 - [1-2] ENTR - Fluor - ce-mMaple3(1 intron, syntrons(3), no_atg, no_stop)

Plasmid

#159865

Purpose

Three-fragment Gateway compatible flourophore for expression in C. elegans

Depositor

Christian Froekjaer-Jensen

Publication

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

[1-2] ENTR - Fluor - ce-mMaple3(1 intron, syntrons(3), no_atg, no_stop)

Expression

Worm

Mutation

Not applicable

Available since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pJW4.28

Plasmid

#62878

Purpose

Bacterial expression of MBP-MEG-3 T541A S582A T605A

Depositor

Geraldine Seydoux

Publication

Wang et al Elife. 2014 Dec 23;4. doi: 10.7554/eLife

Insert

MBP-MEG-3 T541A S582A T605A

Tags

MBP

Expression

Bacterial

Mutation

T541A S582A T605A

Available since

March 30, 2015

Availability

Academic Institutions and Nonprofits only

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pNH066

Plasmid

#42162

Depositor

Colin Dolphin

Publication

Hirani et al BMC Biotechnol. 2013 Jan 3;13(1):1.

Insert

N-TAP Tag-RT-::mTFP1

Tags

TAP-TAg

Available since

March 14, 2013

Availability

Academic Institutions and Nonprofits only

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AiP15218 - pAAV-AiE1470q-minBG-SYFP2-WPRE3-BGHpA

Plasmid

#243122

Purpose

AiE1470q is an enhancer sequence designed to drive AAV-mediated transgene expression in specific populations of brain cells.

Depositor

Trygve Bakken, Tanya Daigle, Allen Institute for Brain Science, Boaz Levi, Bosiljka Tasic, Jonathan Ting

Insert

SYFP2

Use

AAV

Available since

Aug. 31, 2026

Availability

Academic Institutions and Nonprofits only

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gMELT-Mito/pcDNA3.1(-)

Plasmid

#258480

Purpose

Expresses a temperature indicator gMELT with a mitochondrial targeting sequence in mammalian cells

Depositor

Takeharu Nagai

Publication

Fukushima et al ACS Sens. 2024 Aug 23;9(8):3889-3897. do

Insert

gMELT-Mito

Expression

Mammalian

Available since

July 14, 2026

Availability

Academic Institutions and Nonprofits only

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AAVS1 BSD EF1a Fluc2 2A GFP

Plasmid

#255457

Purpose

AAVS1 knock-in construct that expresses firefly luciferase, eGFP, and blasticidin deaminase

Depositor

Le Cong

Insert

firefly luciferase

Expression

Mammalian

Available since

May 19, 2026

Availability

Academic Institutions and Nonprofits only

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CMV_4xmts_HaloTag

Plasmid

#251345

Purpose

Mitochondria matrix targeted HaloTag with 4 mitochondria targeting sequences. CMV promoter.

Depositor

Edward Avezov

Publication

Parutto et al Nat Commun. 2026 Feb 11;17(1):2639. doi:

Insert

HaloTag

Expression

Mammalian

Promoter

CMV

Available since

April 22, 2026

Availability

Academic Institutions and Nonprofits only

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pGEM_H1.0_HaloTag

Plasmid

#247483

Purpose

CRISPR/Cas9 donor plasmid to tag human histone H1.0 with Halo tag

Depositor

Kazuhiro Maeshima

Publication

Shimazoe et al Sci Adv. 2026 Apr 10;12(15):eaec9801. do

Insert

H1.0 (H1F0) (H1-0 Human)

Use

CRISPR

Tags

HaloTag

Available since

April 13, 2026

Availability

Academic Institutions and Nonprofits only

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AiP1752 - pAAV-AiE2301m-minBG-SYFP2-WPRE3-BGHpA

Plasmid

#242987

Purpose

AiE2301m is an enhancer sequence designed to drive AAV-mediated transgene expression in specific populations of brain cells.

Depositor

Allen Institute for Brain Science, Bosiljka Tasic

Insert

SYFP2

Use

AAV

Available since

Sept. 12, 2025

Availability

Academic Institutions and Nonprofits only

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pP4-C

Plasmid

#225097

Purpose

gfpUV reporter vector with a high performance adenine-dependent riboswitch

Depositor

Robert Batey

Publication

Drogalis et al PLoS One. 2020 Dec 1;15(12):e0243155. do

Insert

synthetic riboswitch

Expression

Bacterial

Promoter

synthetic constitutive promoter

Available since

Feb. 11, 2025

Availability

Academic Institutions and Nonprofits only

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pRSF-G1(5CNW)RS

Plasmid

#222872

Purpose

tRNA synthetase/tRNA pair for the in vivo incorporation of 5-Cyano-L-Tryptophan (5-CN-Trp) into proteins in E. coli in response to the amber (TAG) codon

Depositor

Thomas Huber

Publication

Qianzhu et al Angew Chem Int Ed Engl. 2024 Dec 4:e2024

Insert

tRNA synthetase

Expression

Bacterial

Promoter

T7

Available since

Dec. 17, 2024

Availability

Academic Institutions and Nonprofits only

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pRSF-G1(6CNW)RS

Plasmid

#222873

Purpose

tRNA synthetase/tRNA pair for the in vivo incorporation of 6-Cyano-L-Tryptophan (6-CN-Trp) into proteins in E. coli in response to the amber (TAG) codon

Depositor

Thomas Huber

Publication

Qianzhu et al Angew Chem Int Ed Engl. 2024 Dec 4:e2024

Insert

tRNA synthetase

Expression

Bacterial

Promoter

T7

Available since

Dec. 17, 2024

Availability

Academic Institutions and Nonprofits only

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pXTm-PH domain

Plasmid

#210326

Purpose

Expresses EGFP with PH domain

Depositor

Li Yu

Publication

Jiang et al Nat Cell Biol. 2019 Aug;21(8):966-977. d

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

PH domain

Use

Fish expression

Available since

Jan. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pGEMT-aid-p2a-GFP

Plasmid

#161976

Purpose

homologous donor template for endogenous tagging

Depositor

Joanna Wysocka

Publication

Gu et al Mol Cell. 2020 Nov 19;80(4):699-711.e7.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

GSlinker-aid-GFP

Available since

Feb. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV Aquamarine-shadowY tandem

Plasmid

#157775

Purpose

Produces a fusion between Aquamarine and shadowY. It can be used as a positive control for FRET (using FLIM)

Depositor

Marc Tramier

Publication

Bertolin et al eLife. 2018 Aug 2;7. pii: 38111. doi: 10

Type

Empty backbone

Tags

Aquamarine and shadowY

Expression

Mammalian

Available since

Feb. 10, 2021

Availability

Academic Institutions and Nonprofits only

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