We narrowed to 4,564 results for: HRE

1. pAc5.1B-lambdaN-HA-DmTral_394-543-V5His6_D

   Plasmid

   #146138

   Purpose

   Insect Expression of DmTral_394-543

   Depositor

   Elisa Izaurralde

   Article

   Tritschler et al Mol Cell Biol. 2008 Nov . 28(21):6695-70

   Insert

   DmTral_394-543 (tral Fly)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   three silent mutations A198C, A387G, A1437G and 4…

   Promoter

   Available since

   Feb. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAc5.1B-lambdaN-HA-DmTral_394-652_D

   Plasmid

   #146139

   Purpose

   Insect Expression of DmTral_394-652

   Depositor

   Elisa Izaurralde

   Article

   Tritschler et al Mol Cell Biol. 2008 Nov . 28(21):6695-70

   Insert

   DmTral_394-652 (tral Fly)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   three silent mutations A198C, A387G, A1437G and 4…

   Promoter

   Available since

   Feb. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pAc5.1B-lambdaN-HA-DmTral-R21EE23K_C

   Plasmid

   #146078

   Purpose

   Insect Expression of DmTral-R21EE23K

   Depositor

   Elisa Izaurralde

   Article

   Tritschler et al Mol Cell Biol. 2008 Nov . 28(21):6695-70

   Insert

   DmTral-R21EE23K (tral Fly)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   three silent mutations A198C, A387G, A1437G and 4…

   Promoter

   Available since

   Feb. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pAc5.1B-lambdaN-HA-DmTral-R21ES13AI15A_C

   Plasmid

   #146079

   Purpose

   Insect Expression of DmTral-R21ES13AI15A

   Depositor

   Elisa Izaurralde

   Article

   Tritschler et al Mol Cell Biol. 2008 Nov . 28(21):6695-70

   Insert

   DmTral-R21ES13AI15A (tral Fly)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   three silent mutations A198C, A387G, A1437G and 4…

   Promoter

   Available since

   Feb. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pAc5.1B-lambdaN-HA-DmTral-R42EF44A_C

   Plasmid

   #146080

   Purpose

   Insect Expression of DmTral-R42EF44A

   Depositor

   Elisa Izaurralde

   Article

   Tritschler et al Mol Cell Biol. 2008 Nov . 28(21):6695-70

   Insert

   DmTral-R42EF44A (tral Fly)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   three silent mutations A198C, A387G, A1437G and 4…

   Promoter

   Available since

   Feb. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pAc5.1B-lambdaN-HA-DmTral-S13AI15A_C

   Plasmid

   #146081

   Purpose

   Insect Expression of DmTral-S13AI15A

   Depositor

   Elisa Izaurralde

   Article

   Tritschler et al Mol Cell Biol. 2008 Nov . 28(21):6695-70

   Insert

   DmTral-S13AI15A (tral Fly)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   three silent mutations A198C, A387G, A1437G and 4…

   Promoter

   Available since

   Feb. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pAc5.1B-lambdaN-HA-DmTral-FDF-ADA_C

   Plasmid

   #146039

   Purpose

   Insect Expression of DmTral-FDF-ADA

   Depositor

   Elisa Izaurralde

   Article

   Tritschler et al Mol Cell. 2009 Mar 13. 33(5):661-8.

   Insert

   DmTral-FDF-ADA (tral Fly)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   FDF to ADA (407-409) mutation. Additionally, thre…

   Promoter

   Available since

   June 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. GFP-Tac-STC(5A)

   Plasmid

   #162491

   Purpose

   Expresses non-O-glycosylated partial length Tac (stem region, TMD and cytosolic tail) with GFP tag in mammalian cells

   Depositor

   Lei Lu

   Article

   Sun et al J Biol Chem. 2020 Aug 21. pii: RA120.014

   Insert

   partial length Tac (stem region with mutations, TMD and cytosolic tail) (IL2RA Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Tac is in partial length with its stem region, TM…

   Promoter

   Available since

   Jan. 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pQE30-VRN1-R249E-R289E-R296E

   Plasmid

   #159532

   Purpose

   Expresses Arabidopsis thaliana VERNALIZATION1 mutant, with R249E, R289E and R296E mutations, in E. coli

   Depositor

   Joshua Mylne

   Article

   King et al J Biol Chem. 2013 Feb 1;288(5):3198-207.

   Insert

   VERNALIZATION1 (VRN1 Mustard Weed)
   

   Use

   Tags

   Six-Histidine tag

   Expression

   Bacterial

   Mutation

   Three charge reversal mutations, namely R249E R28…

   Promoter

   T5

   Available since

   Dec. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. 289aa ELL2

    Plasmid

    #127266

    Purpose

    For in vitro translation of shorter human ELL2 from Met1. Also contains Met133I, M1381I, M186I mutations.

    Depositor

    Christine Milcarek

    Article

    Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi

    Insert

    NH2-ELL2 (Met133ILeu, M1381ILeu, M186ILeu) (ELL2 Human)
    

    Use

    In vitro translation

    Tags

    Expression

    Mutation

    Three Mets (133, 138, 186) to Ileu. Contains 289…

    Promoter

    T7

    Available since

    July 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pJC20cdc8.A18T

    Plasmid

    #99360

    Purpose

    Bacterial expression vector containing cDNA encoding for the fission yeast tropomyosin, Cdc8 with the amino acid substitution Ala-18-Thr.

    Depositor

    Dan Mulvihill

    Article

    Johnson et al Biochem Biophys Res Commun. 2017 Oct 25.

    Insert

    cdc8 (cdc8 Fission Yeast)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Alanine 18 to Threonine

    Promoter

    T7

    Available since

    Oct. 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pJC20cdc8.110

    Plasmid

    #99363

    Purpose

    Bacterial expression vector containing cDNA encoding for the temperature sensitive fission yeast tropomyosin mutant, Cdc8.110.

    Depositor

    Dan Mulvihill

    Article

    Johnson et al Biochem Biophys Res Commun. 2017 Oct 25.

    Insert

    cdc8 (cdc8 Fission Yeast)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Alanine 18 to Threonine and Glutamic acid 31 to L…

    Promoter

    T7

    Available since

    Oct. 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pTH731-2µ-RLuc/minCFLuc

    Plasmid

    #40601

    Depositor

    Tobias von der Haar

    Article

    Chu et al EMBO J. 2014 Jan 1;33(1):21-34. doi: 10.

    Inserts

    Firefly Luciferase
    Renilla luciferase
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    All codons of the original FLuc gene were exchang…

    Promoter

    ADH1 and TDH3

    Available since

    Oct. 30, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pLenti-Tet-MKK6(DD)-Puro

    Plasmid

    #86094

    Purpose

    Tet/Dox inducible (TetR) constitutively active mutant MKK6/MAP2K6 in lentiviral vector

    Depositor

    Cindy Miranti

    Article

    Frank et al J Cell Sci. 2017 Apr 26. pii: jcs.197152

    Insert

    MKK6 (MAP2K6 Human)
    

    Use

    Lentiviral

    Tags

    Myc tag

    Expression

    Mammalian

    Mutation

    Serine 207 and Threonine 211 both changed to Aspa…

    Promoter

    CMV/TetO

    Available since

    Nov. 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pGL4-AARE-luc2P-Hygro

    Plasmid

    #101787

    Purpose

    Luferase reporter plasmid containing three tandem repeats of the amino acid response element (AARE)

    Depositor

    Seiichi Oyadomari

    Article

    Miyake et al FASEB J. 2016 Feb;30(2):798-812. doi: 10

    Insert

    3xAARE
    

    Use

    Luciferase

    Tags

    Luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    minimal TATA-box promoter with low basal activity

    Available since

    Sept. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pcDNA-EGFP MASTL G44S (siRNA resistant)

    Plasmid

    #191012

    Purpose

    Expresses EGFP-tagged kinase-dead MASTL G44S with resistance to MASTL siRNA (ACGCCTTATTCTAGCAAATTA)

    Depositor

    Johanna Ivaska

    Article

    Taskinen et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

    Insert

    Microtubule-associated serine/threonine kinase like (MASTL Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    G44S

    Promoter

    Available since

    Nov. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pMG071 MBP-GSK3β_S9A-HA-His, GST_λPPase

    Plasmid

    #196185

    Purpose

    Co-expresses MBP-GSK3β_S9A-HA-His (human GSK3β with S9A mutation as a fusion protein with MBP, HA, and His-tags) and GST_λPPase in E.coli to produce unphosphorylated MBP-GSK3β_S9A-HA-His

    Depositor

    Jesse Zalatan

    Article

    Gavagan et al Elife. 2023 Aug 7;12:e85444. doi: 10.755

    Inserts

    GSK3B (GSK3B Human)
    λPPase
    

    Use

    Tags

    GST, HA, His6, and MBP

    Expression

    Bacterial

    Mutation

    changed Serine 9 to Alanine

    Promoter

    Tac

    Available since

    Jan. 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pBridge-tyrosinase.σ1A

    Plasmid

    #197381

    Purpose

    Expression of 1) GAL4 DNA-binding domain (BD)-tyrosinase cytosolic tail fusion protein, 2) HA epitope and nuclear localization signal (NLS) AP-1 σ1A fusion protein in yeast (yeast three-hybrid assays)

    Depositor

    Juan Bonifacino

    Article

    Mattera et al J Biol Chem. 2011 Jan 21;286(3):2022-30.

    Inserts

    tyrosinase cytosolic tail
    AP-1 σ1A
    

    Use

    Tags

    GAL4-DNA binding domain fragment, HA tag, and SV4…

    Expression

    Yeast

    Mutation

    Contains an extra 42 nucleotides encoding 14 resi…

    Promoter

    ADH1 and MET25

    Available since

    March 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pTH734-2µ-RLuc/stopCFLuc

    Plasmid

    #40609

    Depositor

    Tobias von der Haar

    Article

    Chu et al EMBO J. 2014 Jan 1;33(1):21-34. doi: 10.

    Inserts

    Firefly Luciferase (nonsense mutant)
    Renilla luciferase
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    All codons of the original FLuc gene were exchang…

    Promoter

    ADH1 and TDH3

    Available since

    Oct. 30, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. Flag-KLF6-4D (1054)

    Plasmid

    #49490

    Purpose

    expresses human Flag tagged KLF6 with 4D mutation

    Depositor

    Scott Friedman

    Article

    Lang et al Oncogene. 2012 Oct 22. doi: 10.1038/onc.

    Insert

    KFL6-4D (KLF6 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    three serines and one tyrosine mutated to aspart…

    Promoter

    CMV

    Available since

    Feb. 18, 2014

    Availability

    Academic Institutions and Nonprofits only