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pDONR223_STK11_WT

Plasmid

#81744

Purpose

Gateway Donor vector containing STK11 , part of the Target Accelerator Plasmid Collection.

Depositor

William Hahn, Jesse Boehm, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

STK11 (STK11 Human)

Use

Gateway entry vector

Tags

Expression

Mutation

WT

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pscALPSpuro-HsACE2 (human)

Plasmid

#158081

Purpose

Expresses human ACE2 in mammalian cells

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Cell. 2020 Sep 15. pii: S0092-8674(20)31

Insert

Human ACE2 (ACE2 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pET30-2-PSAT1

Plasmid

#83908

Purpose

stable overexpression

Depositor

David Sabatini

Article

Pacold et al Nat Chem Biol. 2016 Jun;12(6):452-8. doi

Insert

phosphoserine aminotransferase 1 (PSAT1 Human)

Use

Tags

6x His tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Oct. 21, 2016

Availability

Academic Institutions and Nonprofits only

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pFB1.HMBP.PrS.EZH2

Plasmid

#125161

Purpose

Expresses human EZH2 in insect cells, , under a C3-cleavable (PreScission Protease) N-terminal hexahistidine-MBP tag.

Depositor

Chen Davidovich

Article

Zhang et al Nat Struct Mol Biol. 2019 Mar;26(3):237-

Insert

EZH2 (EZH2 Human)

Use

Baculovirus expression vector

Tags

His-MBP

Expression

Insect

Mutation

Promoter

Available since

April 27, 2020

Availability

Academic Institutions and Nonprofits only

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pDONR223_RASA1_WT

Plasmid

#81778

Purpose

Gateway Donor vector containing RASA1 , part of the Target Accelerator Plasmid Collection.

Depositor

William Hahn, Jesse Boehm, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

RASA1 (RASA1 Human)

Use

Gateway entry vector

Tags

Expression

Mutation

WT

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_XIAP_WT

Plasmid

#82170

Purpose

Gateway Donor vector containing XIAP , part of the Target Accelerator Plasmid Collection.

Depositor

William Hahn, Jesse Boehm, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

XIAP (XIAP Human)

Use

Gateway entry vector

Tags

Expression

Mutation

WT

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_TMPO_WT

Plasmid

#81818

Purpose

Gateway Donor vector containing TMPO , part of the Target Accelerator Plasmid Collection.

Depositor

William Hahn, Jesse Boehm, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

TMPO (TMPO Human)

Use

Gateway entry vector

Tags

Expression

Mutation

WT

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_MTM1_WT

Plasmid

#81908

Purpose

Gateway Donor vector containing MTM1 ,part of the Target Accelerator Plasmid Collection.

Depositor

William Hahn, Jesse Boehm, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

MTM1 (MTM1 Human)

Use

Gateway entry vector

Tags

Expression

Mutation

E410K

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_STK11_p.D194Y

Plasmid

#81401

Purpose

Gateway Donor vector containing STK11 ,part of the Target Accelerator Plasmid Collection.

Depositor

William Hahn, Jesse Boehm, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

STK11 (STK11 Human)

Use

Gateway entry vector

Tags

Expression

Mutation

D194Y

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_SDHA_WT

Plasmid

#82278

Purpose

Gateway Donor vector containing SDHA, part of the Target Accelerator Plasmid Collection.

Depositor

William Hahn, Jesse Boehm, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

SDHA (SDHA Human)

Use

Gateway entry vector

Tags

Expression

Mutation

WT

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_PPARG_WT

Plasmid

#82223

Purpose

Gateway Donor vector containing PPARG , part of the Target Accelerator Plasmid Collection.

Depositor

William Hahn, Jesse Boehm, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

PPARG (PPARG Human)

Use

Gateway entry vector

Tags

Expression

Mutation

WT

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_TNF_WT

Plasmid

#81824

Purpose

Gateway Donor vector containing TNF , part of the Target Accelerator Plasmid Collection.

Depositor

William Hahn, Jesse Boehm, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

TNF (TNF Human)

Use

Gateway entry vector

Tags

Expression

Mutation

WT

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pINSPECT_5'-xrRNA_NLuc_3'-HCV-UTR

Plasmid

#197265

Purpose

Cloning Backbone for enhanced INSPECT expression (5' xrRNA + 3' HCV-UTR). Encodes for intronic IRES-driven NLuc. Plasmid contains FRT-site flanked PuroR-TK cassette. Clone homology arms via BsaI.

Depositor

Gil Westmeyer

Article

Truong et al Nat Cell Biol. 2022 Nov;24(11):1666-1676

Type

Empty backbone

Use

CRISPR, Luciferase, Synthetic Biology, and Unspec…

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 19, 2023

Availability

Academic Institutions and Nonprofits only

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pDONR223_YY1_WT

Plasmid

#82171

Purpose

Gateway Donor vector containing YY1 , part of the Target Accelerator Plasmid Collection.

Depositor

William Hahn, Jesse Boehm, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

YY1 (YY1 Human)

Use

Gateway entry vector

Tags

Expression

Mutation

WT

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pJV136

Plasmid

#124255

Purpose

Lentiviral vector (pRRL) containing KRAS G12V specific T cell receptor, clone 9

Depositor

Stanley Riddell

Article

Veatch et al Cancer Immunol Res. 2019 Jun;7(6):910-92

Insert

T cell receptor (KRAS Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

May 3, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR223_TRAF3_WT

Plasmid

#81755

Purpose

Gateway Donor vector containing TRAF3 , part of the Target Accelerator Plasmid Collection.

Depositor

William Hahn, Jesse Boehm, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

TRAF3 (TRAF3 Human)

Use

Gateway entry vector

Tags

Expression

Mutation

WT

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pCAGGS D614G soluble Spike

Plasmid

#164651

Purpose

Expresses SARS-CoV-2 soluble Spike protein with a D614G mutation

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Cell. 2020 Sep 15. pii: S0092-8674(20)31

Insert

SARS-CoV-2 soluble spike (S SARS-CoV-2)

Use

Tags

HIS

Expression

Mammalian

Mutation

D614G

Promoter

CAG

Available since

Feb. 16, 2021

Availability

Academic Institutions and Nonprofits only
pDONR223_PIK3CD_WT

Plasmid

#82222

Purpose

Gateway Donor vector containing PIK3CD , part of the Target Accelerator Plasmid Collection.

Depositor

William Hahn, Jesse Boehm, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

PIK3CD (PIK3CD Human)

Use

Gateway entry vector

Tags

Expression

Mutation

WT

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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RRL.sin.cPPT.SFFV/Ace2.IRES-neo.WPRE (MT129)

Plasmid

#145840

Purpose

Lentiviral vector genome to ectopically express human Ace2

Depositor

Caroline Goujon

Article

Rebendenne et al J Virol. 2021 Jan 29. pii: JVI.02415-20.

Insert

human Ace2 (ACE2 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

SFFV

Available since

May 13, 2020

Availability

Academic Institutions and Nonprofits only

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pFB1.HMBP.PrS.AEBP2

Plasmid

#125165

Purpose

Expresses human AEBP2 in insect cells, , under a C3-cleavable (PreScission Protease) N-terminal hexahistidine-MBP tag.

Depositor

Chen Davidovich

Article

Zhang et al Nat Struct Mol Biol. 2019 Mar;26(3):237-

Insert

Zinc finger protein AEBP2 (AEBP2 Human)

Use

Tags

His-MBP

Expression

Insect

Mutation

Promoter

Polyhedrin

Available since

April 27, 2020

Availability

Academic Institutions and Nonprofits only

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