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pCSDest2-SpCas9-MT1-NLS-3XHA-NLS

Plasmid

#69221

Purpose

Expresses R1333K mutant SpCas9 in mammalian cell

Depositor

Scot Wolfe

Article

Bolukbasi et al Nat Methods. 2015 Oct 19. doi: 10.1038/n

Insert

SpCas9

Use

CRISPR

Tags

NLS-3XHA-NLS

Expression

Mammalian

Mutation

Arginine 1333 is changed to Lysine

Available Since

Oct. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pCSDest2-SpCas9-MT2-NLS-3XHA-NLS

Plasmid

#69222

Purpose

Expresses R1333S mutant SpCas9 in mammalian cell

Depositor

Scot Wolfe

Article

Bolukbasi et al Nat Methods. 2015 Oct 19. doi: 10.1038/n

Insert

SpCas9

Use

CRISPR

Tags

NLS-3XHA-NLS

Expression

Mammalian

Mutation

Arginine 1333 is changed to Serine

Available Since

Oct. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pDEST15.1-hRPN13 (285-407) (Q337A/Q338A)

Plasmid

#61935

Purpose

Expression of hRPN13 (285-407) (Q337A/Q338A) in E.coli

Depositor

Chris Hill

Article

VanderLinden et al Mol Cell. 2015 Feb 17. pii: S1097-2765(1

Insert

hRPN13 (285-407) (ADRM1 Human)

Tags

GST

Expression

Bacterial

Mutation

Construct starts at residue 285, residues Q337 an…

Promoter

T7

Available Since

Oct. 9, 2015

Availability

Academic Institutions and Nonprofits only
PcDNA-DEST53-CypB-Del2 N-term GFP

Plasmid

#36133

Depositor

Patrick Van Oostveldt

Article

Dieriks et al Int J Mol Med. 2012 Mar 13. doi: 10.3892

Insert

cyclophilin B (PPIB Human)

Tags

GFP

Expression

Mammalian

Mutation

contains CypB AA 38-210

Promoter

CMV

Available Since

Nov. 1, 2012

Availability

Academic Institutions and Nonprofits only
PcDNA-DEST53-CypB-Del1 N-term GFP

Plasmid

#36131

Depositor

Patrick Van Oostveldt

Article

Dieriks et al Int J Mol Med. 2012 Mar 13. doi: 10.3892

Insert

cyclophilin B (PPIB Human)

Tags

GFP

Expression

Mammalian

Mutation

contains amino acids 37-216 of cyclophilin B; F21…

Promoter

CMV

Available Since

Nov. 1, 2012

Availability

Academic Institutions and Nonprofits only
PcDNA-DEST53-CypB-Nucl2 N-term GFP

Plasmid

#36125

Depositor

Patrick Van Oostveldt

Article

Dieriks et al Int J Mol Med. 2012 Mar 13. doi: 10.3892

Insert

cyclophilin B (PPIB Human)

Tags

GFP

Expression

Mammalian

Mutation

contains CypB AA 34-211

Promoter

CMV

Available Since

Nov. 1, 2012

Availability

Academic Institutions and Nonprofits only
PcDNA-DEST53-CypB-Del3 N-term GFP

Plasmid

#36135

Depositor

Patrick Van Oostveldt

Article

Dieriks et al Int J Mol Med. 2012 Mar 13. doi: 10.3892

Insert

cyclophilin B (PPIB Human)

Tags

GFP

Expression

Mammalian

Mutation

contains amino acids 39-210 of cyclophilin B

Promoter

CMV

Available Since

Nov. 1, 2012

Availability

Academic Institutions and Nonprofits only
pAAV-Tet-Off-FLEX-DEST (JDW 1186)

Plasmid

#229820

Purpose

An AAV, tet-off, gateway-compatible destination vector with cre-dependent expression of the insert. EFS driving destablized rTA

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Type

Empty backbone

Use

AAV

Available Since

Feb. 26, 2025

Availability

Academic Institutions and Nonprofits only

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pHR-ON VIPER CAR (anti-CD19)-final design

Plasmid

#192117

Purpose

Lentiviral deliviery of ON VIPER CAR (anti-CD19)-final design

Depositor

Wilson Wong

Article

Li et al Cancer Cell. 2022 Aug 26. pii: S1535-610

Insert

pHR-pEF1a-VIPER CAR (anti-CD19scFV_CD28_NS3_41BB_CD3z)

Use

Lentiviral

Available Since

March 6, 2023

Availability

Academic Institutions and Nonprofits only

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MTK Lentivirus Multi Cassette Destination Vector (pAN2424)

Plasmid

#194221

Purpose

Backbone for constructing multi casette expression plasmids using the Mammalian Tookit

Depositor

Hana El-Samad

Article

Weinberg et al bioRxiv [Preprint]. 2021 Apr 20:2021.04.

Type

Empty backbone

Use

Lentiviral

Available Since

Sept. 10, 2024

Availability

Academic Institutions and Nonprofits only

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