We narrowed to 7,509 results for: PAC;

1. pUE1-TSP-ORF68 R443A

   Plasmid

   #162652

   Purpose

   Expresses ORF68 R443A in mammalian cells with an N-terminal TwinStrep tag and HRV 3C protease cleavage site

   Depositor

   Britt Glaunsinger

   Article

   Didychuk et al Elife. 2021 Feb 8;10. pii: 62261. doi: 1

   Insert

   ORF68
   

   Use

   Tags

   N-terminal 2xStrep-Tag II and HRV 3C site

   Expression

   Mammalian

   Mutation

   Amino acid R443 mutated to alanine

   Promoter

   CMV

   Available since

   Feb. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pYPQCas12j2

   Plasmid

   #189780

   Purpose

   Gateway entry plasmid (attL1 & attR5) expressing NLS-Cas12j2-NLS, without promoter

   Depositor

   Yiping Qi

   Article

   Liu et al Plant Commun. 2022 Sep 20:100453. doi: 1

   Insert

   Cas12j2
   

   Use

   CRISPR; Gateway compatible cas12j2 entry clone

   Tags

   NLS

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Oct. 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSD117A

   Plasmid

   #218141

   Purpose

   Expresses AviTag-6xHis-MBP-TEV-SD40 for protein purification from E. coli

   Depositor

   David Liu

   Article

   Mercer et al Science. 2024 Mar 15;383(6688):eadk4422.

   Insert

   SD40
   

   Use

   Tags

   AviTag-6xHis-MBP-TEV-(GGGGS)2

   Expression

   Mutation

   Promoter

   Available since

   June 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBEAST-pBen-sfGFP

   Plasmid

   #114598

   Purpose

   "Output expression of sfGFP under the activation of BenR transcription factor for cell-free expression"

   Depositor

   Jerome Bonnet

   Article

   Voyvodic et al Nat Commun. 2019 Apr 12;10(1):1697. doi:

   Insert

   sfGFP
   

   Use

   Synthetic Biology; Cell-free protein synthesis

   Tags

   Expression

   Mutation

   Promoter

   PBen

   Available since

   April 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET-CVm-PTEN

   Plasmid

   #168032

   Purpose

   Expresses eCFP-PTEN(M265-N329)-mVenus in bacterial cells

   Depositor

   David Liu

   Article

   Blum et al Science. 2021 Feb 19;371(6531):803-810.

   Insert

   eCFP-PTEN(M265-N329)-mVenus
   

   Use

   Tags

   Hisx6 and myc

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBEAST-BenR

   Plasmid

   #114597

   Purpose

   "Strong constitutive expression of transcription factor, BenR, for cell-free expression"

   Depositor

   Jerome Bonnet

   Article

   Voyvodic et al Nat Commun. 2019 Apr 12;10(1):1697. doi:

   Insert

   BenR
   

   Use

   Synthetic Biology; Cell-free protein synthesis

   Tags

   Expression

   Mutation

   Promoter

   OR2-OR1-Pr

   Available since

   April 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-syn-IRES-hrGFP

   Plasmid

   #107549

   Purpose

   high transduction efficiency AAV-mediated synapsin promoter-dependent expression IRES-hrGFP

   Depositor

   Hélène Marie

   Article

   Pousinha et al Elife. 2017 Jul 6;6. doi: 10.7554/eLife.

   Type

   Empty backbone

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 1, 2018

   Availability

   Academic Institutions and Nonprofits only

8. pGG184

   Plasmid

   #165604

   Purpose

   Reporter plasmid for B1H-dependent expression of the HIS3/GFP operon with a single hEGFP protospacer: PS1(upstream of promoter with 'CAGCG' PAM)-lac-HIS3 and GFP

   Depositor

   Marcus Noyes

   Article

   Goldberg et al Nat Commun. 2021 Jan 13;12(1):349. doi:

   Insert

   hEGFP protospacer ('CAGCG' PAM) upstream of the HIS3/GFP promoter
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   'CAGCG' PAM replacing the 'CGGCG&#…

   Promoter

   lac

   Available since

   June 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pRSFDuet-1_StefinA

   Plasmid

   #182402

   Purpose

   Bacterial expression of stefinA

   Depositor

   Dan Mulvihill

   Article

   Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

   Insert

   stefin (CSTA Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Feb. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. SmtA Synechococcus elongatus PCC 7942 Metallothionein

    Plasmid

    #200298

    Purpose

    Bacterial expression plasmids for production of recombinant Synechococcus elongatus PCC 7942 Metallothionein

    Depositor

    Artur Krężel

    Article

    Mosna et al Metallomics. 2023 Oct 4;15(10):mfad061.

    Insert

    Gene of metallothionein with Chiting Binding Domanin-Intein (SYNPCC7942_RS13750 Synechococcus elongatus)
    

    Use

    Tags

    Chiting Binding Domanin-Intein

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMOD_B2517

    Plasmid

    #91074

    Purpose

    Module B, Promoter: TaU3, Gene: Esp3I ccdb cassette for single gRNA spacer cloning, Terminator: Pol III

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    Esp3I ccdb cassette for single gRNA spacer cloning
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    TaU3

    Available since

    July 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. p2T-CMV-HNHx-ABEmax7.10HD-BlastR

    Plasmid

    #176477

    Purpose

    A-to-G base editor with alternative targeting scope

    Depositor

    Gerald Schwank

    Article

    Villiger et al Mol Ther Nucleic Acids. 2021 Aug 26;26:5

    Insert

    HNHx-ABEmax7.10HD
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMXs_FLAG-SFXN3

    Plasmid

    #110637

    Purpose

    Retroviral expression of Flag-SFXN3 in mammalian cells

    Depositor

    David Sabatini

    Article

    Kory et al Science. 2018 Nov 16;362(6416). pii: 362

    Insert

    SFXN3 (SFXN3 Human)
    

    Use

    Retroviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pPB-CAG-Flag3-pG-mNanog-pgk-hph

    Plasmid

    #74911

    Purpose

    Mammalian expression of Flag tagged mNanog

    Depositor

    Jose Silva

    Article

    Theunissen et al Development. 2011 Nov;138(22):4853-65. d

    Insert

    Nanog (Nanog Mouse)
    

    Use

    Tags

    3xFlag

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    May 17, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMXs_SFXN5

    Plasmid

    #110640

    Purpose

    Retroviral expression of SFXN5 in mammalian cells

    Depositor

    David Sabatini

    Article

    Kory et al Science. 2018 Nov 16;362(6416). pii: 362

    Insert

    SFXN5 (SFXN5 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pC031 - RcrCas13a from Rhodobacter capsulatus R121

    Plasmid

    #91921

    Purpose

    Expresses RcrCas13a for bacterial expression and contains backbone for spacer cloning

    Depositor

    Feng Zhang

    Article

    Abudayyeh et al Nature. 2017 Oct 4. doi: 10.1038/nature2

    Insert

    RcrCas13a and guide expression cassette
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pJOE5751.1

    Plasmid

    #135083

    Purpose

    pBR322-based L-rhamnose-inducible vector.

    Depositor

    Josef Altenbuchner

    Article

    Hoffmann et al FEBS J. 2012 Oct;279(20):3911-24. doi: 1

    Insert

    P-rhaP-BAD-His tag-eGFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pC027 - LweCas13a from Listeria weihenstephanensis FSL R9-0317

    Plasmid

    #91917

    Purpose

    Expresses LweCas13a for bacterial expression and contains backbone for spacer cloning

    Depositor

    Feng Zhang

    Article

    Abudayyeh et al Nature. 2017 Oct 4. doi: 10.1038/nature2

    Insert

    LweCas13a and guide expression cassette
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pOPO635

    Plasmid

    #195297

    Purpose

    pACYC-plac-MycaTnsABC, lac promoter expressing transposase genes TnsABC of McCAST (Tn7575).

    Depositor

    Joseph Peters

    Article

    Hsieh et al Nucleic Acids Res. 2023 Jan 25;51(2):765

    Insert

    TnsABC operon of McCAST (Tn7575)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    IPTG inducible lac promoter

    Available since

    May 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pSIN-Pdha1

    Plasmid

    #175936

    Purpose

    knockout mouse Pdha1

    Depositor

    Dan Littman

    Article

    Wu et al Cell. 2020 Aug 6;182(3):641-654.e20. doi

    Insert

    Pdha1 gRNA (Pdha1 Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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