We narrowed to 12,157 results for: pcDNA
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Plasmid#231635PurposeMammalian expression of a flag-tagged full-length version of the mouse chaperone protein proSAAS.DepositorAvailable SinceFeb. 5, 2025AvailabilityAcademic Institutions and Nonprofits only
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TadA-8e-eIscBn-C-HMG-D
Plasmid#227145PurposeVector encoding human codon-optimized enhanced-activity nOgeuIscB fusion with TadA8e and HMG-D domain in C terminus driven by CMV promoterDepositorInsertbpNLS-TadA-8e-linker-nOgeuIscB-v3-linker-HMG-D-bpNLS
MutationD96R/E84R/V159R/H339APromoterCMVAvailable SinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
eIscB-D
Plasmid#227017PurposeVector encoding human codon-optimized enhanced-activity OgeuIscB fusion with HMD-D domain and extra SV40 NLS driven by CMV promoterDepositorInsertSV40 NLS-HMG-D-linker-OgeuIscB-v3-SV40-nucleoplasmin NLS
MutationD96R/E84R/V159RPromoterCMVAvailable SinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
hA3A*-IscBn
Plasmid#227146PurposeVector encoding human codon-optimized nOgeuIscB fusion with two copies of UGI and hA3A* variant driven by CMV promoterDepositorInsertbpNLS-hA3A*-linker-nOgeuIscB-nucleoplasmin NLS-2XUGI-bpNLS
MutationD96R/E84R/V159R/H339APromoterCMVAvailable SinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
TadA-8e-eIscBn-N-HMG-D
Plasmid#227143PurposeVector encoding human codon-optimized enhanced-activity nOgeuIscB fusion with TadA8e and HMG-D domain in N terminus driven by CMV promoterDepositorInsertbpNLS-HMG-D-linker-TadA-8e-linker-nOgeuIscB-v3-bpNLS
MutationD96R/E84R/V159R/H339APromoterCMVAvailable SinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
L2117-FKBP-GPA-114-RH-CyOFP1
Plasmid#222884PurposeBiosensor chain detecting rapamycin and responding with BRET. FKBP ectodomain, human Glycophorin A (GPA) scaffold, 20 GS linker, split Nanoluciferase fragment 114, RH linker, CyOFP1 protein.DepositorInsertFKBP-GPA-20GS-NanoLuc114-RH-CyOFP1
TagsMycExpressionMammalianPromoterCMVAvailable SinceSept. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
L2116-FRB-GPA-114-RH-CyOFP1
Plasmid#222883PurposeBiosensor chain detecting rapamycin and responding with BRET. FRB ectodomain, human Glycophorin A (GPA) scaffold, 20 GS linker, split Nanoluciferase fragment 114, RH linker, CyOFP1 protein.DepositorInsertFRB-GPA-20GS-NanoLuc114-RH-CyOFP1
TagsMycExpressionMammalianPromoterCMVAvailable SinceSept. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
L2379-FKBP-GPA(V84R)-20-11S
Plasmid#222901PurposeBiosensor chain detecting rapamycin and responding with split Nanoluciferase reconstitution. FKBP ectodomain, human Glycophorin A (GPA) (V84R) scaffold, 20 GS linker, split Nanoluciferase fragment 11SDepositorInsertFKBP-GPA(V84R)-20GS-NanoLuc11S
TagsMycExpressionMammalianMutationV84R in GPAPromoterCMVAvailable SinceAug. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
L2380-FKBP-GPA(V84R)-20-114
Plasmid#222900PurposeBiosensor chain detecting rapamycin and responding with split Nanoluciferase reconstitution. FKBP ectodomain, human Glycophorin A (GPA) (V84R) scaffold, 20 GS linker, split Nanoluciferase fragment 114DepositorInsertFKBP-GPA(V84R)-20GS-NanoLuc114
TagsMycExpressionMammalianMutationV84R in GPAPromoterCMVAvailable SinceAug. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
L2378-FRB-GPA(V84R)-20-11S
Plasmid#222899PurposeBiosensor chain detecting rapamycin and responding with split Nanoluciferase reconstitution. FRB ectodomain, human Glycophorin A (GPA) (V84R) scaffold, 20 GS linker, split Nanoluciferase fragment 11S.DepositorInsertFRB-GPA(V84R)-20GS-NanoLuc11S
TagsMycExpressionMammalianMutationV84R in GPAPromoterCMVAvailable SinceAug. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
L2377-FRB-GPA(V84R)-20-114
Plasmid#222898PurposeBiosensor chain detecting rapamycin and responding with split Nanoluciferase reconstitution. FRB ectodomain, human Glycophorin A (GPA) (V84R) scaffold, 20 GS linker, split Nanoluciferase fragment 114.DepositorInsertFRB-GPA(V84R)-20GS-NanoLuc114
TagsMycExpressionMammalianMutationV84R in GPAPromoterCMVAvailable SinceAug. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
L2132-FKBP-GPA-114-5-CyOFP1
Plasmid#222891PurposeBiosensor chain detecting rapamycin and responding with BRET. FKBP ectodomain, human Glycophorin A (GPA) scaffold, 20 GS linker, split Nanoluciferase fragment 114, 5 GS linker, CyOFP1 protein.DepositorInsertFKBP-GPA-20GS-NanoLuc114-5GS-CyOFP1
TagsMycExpressionMammalianPromoterCMVAvailable SinceJuly 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
L2127-FRB-GPA-114-20-CyOFP1
Plasmid#222886PurposeBiosensor chain detecting rapamycin and responding with BRET. FRB ectodomain, human Glycophorin A (GPA) scaffold, 20 GS linker, split Nanoluciferase fragment 114, 20 GS linker, CyOFP1 protein.DepositorInsertFRB-GPA-20GS-NanoLuc114-20GS-CyOFP1
TagsMycExpressionMammalianPromoterCMVAvailable SinceJuly 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
L2129-FRB-GPA-114-5-CyOFP1
Plasmid#222888PurposeBiosensor chain detecting rapamycin and responding with BRET. FRB ectodomain, human Glycophorin A (GPA) scaffold, 20 GS linker, split Nanoluciferase fragment 114, 5 GS linker, CyOFP1 protein.DepositorInsertFRB-GPA-20GS-NanoLuc114-5GS-CyOFP1
TagsMycExpressionMammalianPromoterCMVAvailable SinceJuly 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
L2128-FRB-GPA-114-10-CyOFP1
Plasmid#222887PurposeBiosensor chain detecting rapamycin and responding with BRET. FRB ectodomain, human Glycophorin A (GPA) scaffold, 20 GS linker, split Nanoluciferase fragment 114, 10 GS linker, CyOFP1 protein.DepositorInsertFRB-GPA-20GS-NanoLuc114-10GS-CyOFP1
TagsMycExpressionMammalianPromoterCMVAvailable SinceJuly 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
L2131-FKBP-GPA-114-10-CyOFP1
Plasmid#222890PurposeBiosensor chain detecting rapamycin and responding with BRET. FKBP ectodomain, human Glycophorin A (GPA) scaffold, 20 GS linker, split Nanoluciferase fragment 114, 10 GS linker, CyOFP1 protein.DepositorInsertFKBP-GPA-20GS-NanoLuc114-10GS-CyOFP1
TagsMycExpressionMammalianPromoterCMVAvailable SinceJuly 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
L2130-FKBP-GPA-114-20-CyOFP1
Plasmid#222889PurposeBiosensor chain detecting rapamycin and responding with BRET. FKBP ectodomain, human Glycophorin A (GPA) scaffold, 20 GS linker, split Nanoluciferase fragment 114, 20 GS linker, CyOFP1 protein.DepositorInsertFKBP-GPA-20GS-NanoLuc114-20GS-CyOFP1
TagsMycExpressionMammalianPromoterCMVAvailable SinceJuly 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
KU70-eGFP-SUN1ΔN
Plasmid#213516PurposeExpresses human KU70-eGFP-SUN1ΔN in mammalian cellsDepositorInsertKU70-eGFP-SUNΔN
ExpressionMammalianMutationSUN1ΔN has the first 138 amino acid (lamina domai…PromoterCMVAvailable SinceMay 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
NSP4-Δ220-234-Flag
Plasmid#210341PurposeExpresses SARS-CoV-2 NSP4-Δ220-234 fused with Flag in mammalian cellsDepositorInsertSARS-CoV-2 NSP4 (ORF1ab Human, SARS-CoV-2 isolate Wuhan-Hu-1)
TagsFlagExpressionMammalianMutationΔ220-234Available SinceJan. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
NSP4-H120N/F121L-Flag
Plasmid#210340PurposeExpresses SARS-CoV-2 NSP4-H120N/F121L fused with Flag in mammalian cellsDepositorInsertSARS-CoV-2 NSP4 (ORF1ab Human, SARS-CoV-2 isolate Wuhan-Hu-1)
TagsFlagExpressionMammalianMutationH120N/F121LAvailable SinceJan. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
NSP4-FR-2Strep
Plasmid#210316PurposeExpresses SARS-CoV-2 NSP4 in mammalian cellsDepositorInsertSARS-CoV-2 NSP4 (ORF1ab Human, SARS-CoV-2 isolate Wuhan-Hu-1)
Tags2StrepExpressionMammalianMutationMany synonymous changes due to codon optimizationAvailable SinceJan. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
CCM2_I432D_eGFP
Plasmid#208879PurposeExpress eGFP-CCM2 with the I432D mutation in mammalian cells.DepositorAvailable SinceDec. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
CCM2_W421AD422A_eGFP
Plasmid#208880PurposeExpress eGFP-CCM2 with the W421A and D422A mutations in mammalian cells.DepositorAvailable SinceDec. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
CCM2_I428S_eGFP
Plasmid#208878PurposeExpress eGFP-CCM2 with the I428S mutation in mammalian cells.DepositorAvailable SinceDec. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
STIM1-2Strep
Plasmid#210361PurposeExpresses STIM1 fused with 2Strep in mammalian cellsDepositorAvailable SinceDec. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
FKBP10-2Strep
Plasmid#210357PurposeExpresses FKBP10 fused with 2Strep in mammalian cellsDepositorAvailable SinceDec. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
DNAJC11-2Strep
Plasmid#210354PurposeExpresses DNAJC11 fused with 2Strep in mammalian cellsDepositorAvailable SinceDec. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
mCherry-NIP45 wt
Plasmid#210266PurposeInducible expression of N-terminally mCherry-tagged NIP45 wt resistant to siRNA. Use with Flp-In T-Rex system.DepositorInsertNIP45 wt siRES (NFATC2IP Synthetic)
TagsmCherryExpressionMammalianMutationsiRESPromoterCMV/TetO2Available SinceDec. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
EGFP-NIP45 wt
Plasmid#210262PurposeInducible expression of N-terminally EGFP-tagged NIP45 wt resistant to siRNA. Use with Flp-In T-Rex system.DepositorInsertNIP45 wt siRES (NFATC2IP Synthetic)
TagsEGFPExpressionMammalianMutationsiRESPromoterCMV/TetO2Available SinceDec. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMX1463
Plasmid#208805PurposeExpresses plasma-membrane-bound FRB-SBFP2 for rapid relocalization of cytoplasmic proteins tagged with FKBP upon addition of rapamycinDepositorAvailable SinceNov. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
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pvLEA22mer_shuffle_3_mCh-2xFKBP (pBS1119)
Plasmid#185314PurposeFor the mammalian expression of the sleeping chironomid protein pvLEA22mer_shuffle_3 attached to 2xFKBP. The FKBP can be used for inducible dimerization and condensate formationDepositorInsertpvLEA22mer_shuffle_3
ExpressionMammalianAvailable SinceJuly 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTS2308-Tier1-pGL3-PPGK-NLuc_TB
Plasmid#169551PurposeTier-1 expression vector encoding PmPGK1-driven secreted Nluc coupled to fluorescent reporter mTagBFP2 that is insulated via a 5' insulator sequence derived from the pGL3 plasmid (pGL3-PmPGK1-IgkSS-Nluc-p2A-mTagBFP2-pA).DepositorInsertpGL3-insulator-modulated PPGK-driven NanoLuc Luciferase - mTagBFP expression cassette
ExpressionMammalianPromoterPPGKAvailable SinceJune 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
ApoE3_D125I_mCh-2xFKBP (pBS1157)
Plasmid#185330PurposeFor the mammalian expression of the human protein ApoE3_D125I attached to 2xFKBP. The FKBP can be used for inducible dimerization and condensate formationDepositorInsertApoE3_D125I
ExpressionMammalianMutationD125IAvailable SinceJune 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
ApoE3_mCh-2xFKBP (pBS1156)
Plasmid#185329PurposeFor the mammalian expression of the human protein ApoE3 attached to 2xFKBP. The FKBP can be used for inducible dimerization and condensate formationDepositorInsertApoE3
ExpressionMammalianAvailable SinceJune 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
CAHS1_PARRC_101-189_mCh-2xFKBP (pBS1136)
Plasmid#185320PurposeFor the mammalian expression of the tardigrade protein CAHS1_PARRC_101-189 attached to 2xFKBP. The FKBP can be used for inducible dimerization and condensate formationDepositorInsertCAHS1_PARRC_101-189
ExpressionMammalianAvailable SinceJune 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
SYUA_HUMAN_mCh-2xFKBP (pBS1135)
Plasmid#185319PurposeFor the mammalian expression of the human protein SYUA_HUMAN attached to 2xFKBP. The FKBP can be used for inducible dimerization and condensate formationDepositorInsertSYUA_HUMAN
ExpressionMammalianAvailable SinceJune 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pvLEA22mer_mutant_3_mCh-2xFKBP (pBS1123)
Plasmid#185318PurposeFor the mammalian expression of the sleeping chironomid protein pvLEA22mer_mutant_3 attached to 2xFKBP. The FKBP can be used for inducible dimerization and condensate formationDepositorInsertpvLEA22mer_mutant_3
ExpressionMammalianAvailable SinceJune 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
PvLEA4_repeats_k5_1_mCh-2xFKBP (pBS1122)
Plasmid#185317PurposeFor the mammalian expression of the sleeping chironomid protein PvLEA4_repeats_k5_1 attached to 2xFKBP. The FKBP can be used for inducible dimerization and condensate formationDepositorInsertPvLEA4_repeats_k5_1
ExpressionMammalianAvailable SinceJune 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pvLEA22mer_mutant_10_mCh-2xFKBP (pBS1121)
Plasmid#185316PurposeFor the mammalian expression of the sleeping chironomid protein pvLEA22mer_mutant_10 attached to 2xFKBP. The FKBP can be used for inducible dimerization and condensate formationDepositorInsertpvLEA22mer_mutant_10
ExpressionMammalianAvailable SinceJune 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
CAHS2_PARRC_98-130_mCh-2xFKBP (pBS1120)
Plasmid#185315PurposeFor the mammalian expression of the tardigrade protein CAHS2_PARRC_98-130 attached to 2xFKBP. The FKBP can be used for inducible dimerization and condensate formationDepositorInsertCAHS2_PARRC_98-130
ExpressionMammalianAvailable SinceJune 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
PvLEA4_repeats_k3_26_mCh-2xFKBP (pBS1118)
Plasmid#185313PurposeFor the mammalian expression of the sleeping chironomid protein PvLEA4_repeats_k3_26 attached to 2xFKBP. The FKBP can be used for inducible dimerization and condensate formationDepositorInsertPvLEA4_repeats_k3_26
ExpressionMammalianAvailable SinceJune 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
DHN_VrDHN1a_mCh-2xFKBP (pBS1117)
Plasmid#185312PurposeFor the mammalian expression of the riverbank grape plant protein DHN_VrDHN1a attached to 2xFKBP. The FKBP can be used for inducible dimerization and condensate formationDepositorInsertDHN_VrDHN1a
ExpressionMammalianAvailable SinceJune 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
DSUP_RAMVA_1-208_mCh-2xFKBP (pBS1116)
Plasmid#185311PurposeFor the mammalian expression of the tardigrade protein DSUP_RAMVA_1-208 attached to 2xFKBP. The FKBP can be used for inducible dimerization and condensate formationDepositorInsertDSUP_RAMVA_1-208
ExpressionMammalianAvailable SinceJune 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
DHR93_Nccapped_mCh-2xFKBP (pBS1115)
Plasmid#185310PurposeFor the mammalian expression of the synthetic protein DHR93_Nccapped attached to 2xFKBP. The FKBP can be used for inducible dimerization and condensate formationDepositorInsertDHR93_Nccapped
ExpressionMammalianAvailable SinceJune 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
ApoE3_47-300_mCh-2xFKBP (pBS1113)
Plasmid#185308PurposeFor the mammalian expression of the human protein ApoE3_47-300 attached to 2xFKBP. The FKBP can be used for inducible dimerization and condensate formationDepositorInsertApoE3_47-300
ExpressionMammalianAvailable SinceJune 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
ApoE4_47-300_mCh-2xFKBP (pBS1112)
Plasmid#185307PurposeFor the mammalian expression of the human protein ApoE4_47-300 attached to 2xFKBP. The FKBP can be used for inducible dimerization and condensate formationDepositorInsertApoE4_47-300
ExpressionMammalianAvailable SinceJune 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
ApoE2_47-300_mCh-2xFKBP (pBS1111)
Plasmid#185306PurposeFor the mammalian expression of the human protein ApoE2_47-300 attached to 2xFKBP. The FKBP can be used for inducible dimerization and condensate formationDepositorInsertApoE2_47-300
ExpressionMammalianAvailable SinceJune 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
CAHS3hypdu-mCh-2xFKBP (pBS1110)
Plasmid#185305PurposeFor the mammalian expression of the tardigrade protein CAHS3hypdu attached to 2xFKBP. The FKBP can be used for inducible dimerization and condensate formationDepositorInsertCAHS3hypdu
ExpressionMammalianAvailable SinceJune 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRD400
Plasmid#163107PurposeExpression of eYFP_H-NSdbd in eukaryotes for visualisation of nuclear DNADepositorInsertNLS_eYFP_H-NSdbd_NLS
UseEukaryotic expressionExpressionMammalianPromoterCMV promoter; T7 promoterAvailable SinceOct. 29, 2021AvailabilityAcademic Institutions and Nonprofits only