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NAX_CAGGS_CAS9_m4_KRAB-MeCP2_EYFP

Plasmid

#167898

Purpose

PiggyBac compatible plasmid expressing dCas9-KRAB-MeCP2 expression plasmid

Depositor

Alejandro Chavez, Nan Cher Yeo

Article

Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

Insert

dCas9-KRAB-MeCP2

Use

CRISPR

Mutation

D10A, D839A, H840A, N863A

Available Since

Sept. 14, 2022

Availability

Academic Institutions and Nonprofits only

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miR-34aM2

Plasmid

#50828

Purpose

luciferase reporter for human miR-34a

Depositor

Yun Bai, Xueqing Xu

Article

Li et al BMC Mol Biol. 2012 Jan 31;13:4.

Insert

miR-34A promoter M2 (MIR34A Human)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Mutation

second NF-kB binding site mutated (-511)

Promoter

miR34a

Available Since

April 9, 2014

Availability

Academic Institutions and Nonprofits only
MAC-SARS-CoV-2 NSP16

Plasmid

#158367

Purpose

MAC-tagged gene expression

Depositor

Markku Varjosalo

Article

Liu et al Nat Protoc. 2020 Aug 10. pii: 10.1038/s4

Insert

SARS-CoV-2 NSP16 (ORF1ab )

Tags

MAC-tag

Expression

Mammalian

Available Since

Aug. 25, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pRS315-ADH700p-yeCherry-p150-yeGFP-CYCt

Plasmid

#194519

Purpose

Dual fluorescence reporter for studying protein degradation

Depositor

Tommer Ravid

Article

Mashahreh et al Nat Commun. 2022 Dec 8;13(1):7588. doi:

Insert

TIF4631 leader sequence

Expression

Yeast

Available Since

Feb. 15, 2023

Availability

Academic Institutions and Nonprofits only

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GFP-Myo 1d

Plasmid

#134906

Purpose

Expression of rat Myo 1d (Myr 4) in mammalian cells.

Depositor

Martin Bähler

Article

Bahler et al J Cell Biol. 1994 Jul;126(2):375-89. doi

Insert

Myo 1d (rat) with 3' UTR (Myo1d Rat)

Tags

EGFP

Expression

Mammalian

Promoter

CMV IE

Available Since

Jan. 28, 2020

Availability

Academic Institutions and Nonprofits only

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MAC-SARS-CoV-2 Orf8

Plasmid

#158377

Purpose

MAC-tagged gene expression

Depositor

Markku Varjosalo

Article

Liu et al Nat Protoc. 2020 Aug 10. pii: 10.1038/s4

Insert

SARS-CoV-2 Orf8 (ORF8 )

Tags

MAC-tag

Expression

Mammalian

Available Since

Aug. 25, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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MAC-SARS-CoV-2 Orf7a

Plasmid

#158375

Purpose

MAC-tagged gene expression

Depositor

Markku Varjosalo

Article

Liu et al Nat Protoc. 2020 Aug 10. pii: 10.1038/s4

Insert

SARS-CoV-2 Orf7a (ORF7a )

Tags

MAC-tag

Expression

Mammalian

Available Since

Aug. 26, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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lentiGuide-RRBP1-2

Plasmid

#92157

Purpose

CRISPR guide RNA targeting human RRBP1

Depositor

Alice Ting

Article

Hung et al Elife. 2017 Apr 25;6. pii: e24463. doi:

Insert

RRBP1 sgRNA-2

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

Sept. 11, 2018

Availability

Academic Institutions and Nonprofits only

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MAC-SARS-CoV-2 NSP4

Plasmid

#158355

Purpose

MAC-tagged gene expression

Depositor

Markku Varjosalo

Article

Liu et al Nat Protoc. 2020 Aug 10. pii: 10.1038/s4

Insert

SARS-CoV-2 NSP4 (ORF1ab )

Tags

MAC-tag

Expression

Mammalian

Available Since

Nov. 16, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pFLAG-ING5

Plasmid

#13292

Depositor

Curtis Harris

Article

Shiseki et al Cancer Res. 2003 May 15. 63(10):2373-8.

Insert

ING5 (ING5 Human)

Tags

Flag

Expression

Mammalian

Available Since

Oct. 16, 2006

Availability

Academic Institutions and Nonprofits only
pEGFPC1-Ift52

Plasmid

#128875

Purpose

For mammalian expression of mouse Ift52

Depositor

Aimin Liu

Article

Ye et al Proc Natl Acad Sci U S A. 2014 Feb 11;11

Insert

Ift52 (Ift52 Mouse)

Tags

EGFP

Expression

Mammalian

Available Since

July 31, 2019

Availability

Academic Institutions and Nonprofits only

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MAC-SARS-CoV-2 NSP13

Plasmid

#158364

Purpose

MAC-tagged gene expression

Depositor

Markku Varjosalo

Article

Liu et al Nat Protoc. 2020 Aug 10. pii: 10.1038/s4

Insert

SARS-CoV-2 NSP13 (ORF1ab )

Tags

MAC-tag

Expression

Mammalian

Available Since

Aug. 25, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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L_NES 41kDa

Plasmid

#201347

Purpose

Nucleocytoplasmic transport probe

Depositor

Pere Roca-Cusachs

Article

Andreu et al Nat Cell Biol. 2022 Jun;24(6):896-905. d

Insert

Adeno_NES-EGFP-2PrA

Expression

Mammalian

Available Since

June 28, 2023

Availability

Academic Institutions and Nonprofits only

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NAX_CAGGS_CAS9_m4_VPR_mcherry

Plasmid

#167886

Purpose

PiggyBac compatible plasmid expressing dCas9-VPR

Depositor

Alejandro Chavez, Nan Cher Yeo

Article

Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

Insert

dCas9-VPR

Use

CRISPR

Mutation

D10A, D839A, H840A, N863A

Available Since

Sept. 13, 2022

Availability

Academic Institutions and Nonprofits only
MAC-SARS-CoV-2 NSP9

Plasmid

#158360

Purpose

MAC-tagged gene expression

Depositor

Markku Varjosalo

Article

Liu et al Nat Protoc. 2020 Aug 10. pii: 10.1038/s4

Insert

SARS-CoV-2 NSP9 (ORF1ab )

Tags

MAC-tag

Expression

Mammalian

Available Since

Sept. 1, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pBacT7-S1T1L

Plasmid

#33292

Depositor

Terence Dermody

Article

Kobayashi et al Cell Host Microbe. 2007 Apr 19;1(2):147-

Insert

S1 (MoV1sS1gp2, MoV1sS1gp1 Orthoreovirus T1L)

Expression

Mammalian

Promoter

T7

Available Since

Dec. 5, 2011

Availability

Academic Institutions and Nonprofits only

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MAC-SARS-CoV-2 NSP5

Plasmid

#158356

Purpose

MAC-tagged gene expression

Depositor

Markku Varjosalo

Article

Liu et al Nat Protoc. 2020 Aug 10. pii: 10.1038/s4

Insert

SARS-CoV-2 NSP5 (ORF1ab )

Tags

MAC-tag

Expression

Mammalian

Available Since

Nov. 10, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA-Stra6L-6His

Plasmid

#174215

Purpose

Stra6L-6His (N-Terminal) fusion protein driven by an CMV promotor. May be used to study the murine Vitamin A receptor Stra6L.

Depositor

Adrian Press

Article

Stafast et al ACS Appl. Polym. Mater. 2022

Insert

Stra6L (Stra6l Mouse)

Tags

6-His

Expression

Mammalian

Mutation

non

Promoter

CMV

Available Since

Aug. 25, 2022

Availability

Academic Institutions and Nonprofits only

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MAC-SARS-CoV-2 S

Plasmid

#158371

Purpose

MAC-tagged gene expression

Depositor

Markku Varjosalo

Article

Liu et al Nat Protoc. 2020 Aug 10. pii: 10.1038/s4

Insert

SARS-CoV-2 S (S )

Tags

MAC-tag

Expression

Mammalian

Available Since

Aug. 25, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pLPP5

Plasmid

#209967

Purpose

Contains Level 0 Part: inducible Promoter (PsppQ) with two regulatory genes sppK and sppR for the construction of Level 1 plasmids

Depositor

Thorsten Mascher

Article

Li et al ACS Synth Biol. 2024 Sep 20;13(9):2764-2

Insert

Promoter (P_sppQ)

Expression

Bacterial

Available Since

Sept. 13, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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