We narrowed to 4,564 results for: HRE

1. RKF1 X030_pECIA14

   Plasmid

   #114856

   Purpose

   Prey vector RKF1 X030_pECIA14 should be used with bait vector RKF1 X030_pECIA2.

   Depositor

   Youssef Belkhadir

   Article

   Smakowska-Luzan et al Nature. 2018 Jan 18;553(7688):342-346. d

   Insert

   AT1G29750 (RKF1 Mustard Weed)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Metallothionein (Copper-Inducible)

   Available since

   March 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGL4.23 A82V/T230A/D637G 2014 EBOV Delta-Mucin-Like-Domain

   Plasmid

   #86026

   Purpose

   Ebola Virus (Makona) Glycoprotein in CMV driven mammalian expression vector

   Depositor

   Jeremy Luban, Pardis Sabeti

   Article

   Diehl et al Cell. 2016 Nov 3;167(4):1088-1098.e6. do

   Insert

   Makona Ebola Virus Glycoprotein
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Lacks mucin-like domain; Changed alanine 82 to va…

   Promoter

   CMV IE

   Available since

   July 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pTH752-CEN-RLuc/min103maxCFLuc

   Plasmid

   #38218

   Depositor

   Tobias von der Haar

   Article

   Chu et al EMBO J. 2014 Jan 1;33(1):21-34. doi: 10.

   Inserts

   Firefly Luciferase
   Renilla luciferase
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   The first 103 codons of the original FLuc gene we…

   Promoter

   ADH1 and TDH3 (=GPD)

   Available since

   Oct. 22, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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4. pTH751-CEN-RLuc/min53maxCFLuc

   Plasmid

   #38217

   Depositor

   Tobias von der Haar

   Article

   Chu et al EMBO J. 2014 Jan 1;33(1):21-34. doi: 10.

   Inserts

   Firefly Luciferase
   Renilla luciferase
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   The first 53 codons of the original FLuc gene wer…

   Promoter

   ADH1 and TDH3 (=GPD)

   Available since

   Oct. 22, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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5. psiCHECK-1-BRAF_WT&Mut-SM

   Plasmid

   #215916

   Purpose

   This plasmid encodes luciferase which has siRNA binding sites in 3'UTR for detecting the siRNA silencing activity.

   Depositor

   Kumiko Ui-Tei

   Article

   Song et al Cancers (Basel). 2022 Jun 28;14(13):3162

   Insert

   three tandem repeats of seed-matched (SM) sequence of siBRAF_WT&Mut (BRAF Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. psiCHECK-1-BRAF_WT-SM

   Plasmid

   #215917

   Purpose

   This plasmid encodes luciferase which has siRNA binding sites in 3'UTR for detecting the siRNA silencing activity.

   Depositor

   Kumiko Ui-Tei

   Article

   Song et al Cancers (Basel). 2022 Jun 28;14(13):3162

   Insert

   three tandem repeats of seed-matched (SM) sequence of siBRAF_ WT (BRAF Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. psiCHECK-1-BRAF_Mut1-SM

   Plasmid

   #215918

   Purpose

   This plasmid encodes luciferase which has siRNA binding sites in 3'UTR for detecting the siRNA silencing activity.

   Depositor

   Kumiko Ui-Tei

   Article

   Song et al Cancers (Basel). 2022 Jun 28;14(13):3162

   Insert

   three tandem repeats of seed-matched (SM) sequence of siBRAF_ Mut1 (BRAF Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   BRAF

   Promoter

   Available since

   Oct. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. psiCHECK-1-BRAF_Mut2-SM

   Plasmid

   #215919

   Purpose

   This plasmid encodes luciferase which has siRNA binding sites in 3'UTR for detecting the siRNA silencing activity.

   Depositor

   Kumiko Ui-Tei

   Article

   Song et al Cancers (Basel). 2022 Jun 28;14(13):3162

   Insert

   three tandem repeats of seed-matched (SM) sequence of siBRAF_ Mut2 (BRAF Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   BRAF

   Promoter

   Available since

   Oct. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. AAV-TRE-dSaCas9-NLS-3xHA-3xSunTag_bGHpA

   Plasmid

   #177344

   Purpose

   AAV expression of a catalytically inactive SaCas9 (dSaCas9) fused with three copies of SunTag sequence from doxycycine-inducible promoter

   Depositor

   Naoki Yamamoto

   Article

   Yamamoto et al Research Square [Preprint] 2021. doi: 10

   Insert

   dead hSaCas9
   

   Use

   AAV and CRISPR

   Tags

   3x GCN4 peptide, 3xHA, and NLS

   Expression

   Mammalian

   Mutation

   D10A, N580A

   Promoter

   Tetracycline-dependent promoters

   Available since

   Sept. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. CamKII-LGI1-T380A-pHluorin

    Plasmid

    #185543

    Purpose

    Expresses mutant T380A LGI1-pHluorin under the CamKII promoter

    Depositor

    Jaime de Juan-Sanz

    Article

    Cuhadar et al Cell Rep. 2024 May 28;43(5):114186. doi:

    Insert

    LGI1 (Lgi1 Rat)
    

    Use

    Lentiviral

    Tags

    pHluorin

    Expression

    Mammalian

    Mutation

    changed threonine 380 for alanine

    Promoter

    CamKII

    Available since

    May 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA-EGFP MASTL E167D (siRNA resistant)

    Plasmid

    #191013

    Purpose

    Expresses EGFP-tagged MASTL with E167D mutation and resistance to MASTL siRNA (ACGCCTTATTCTAGCAAATTA)

    Depositor

    Johanna Ivaska

    Article

    Taskinen et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

    Insert

    Microtubule-associated serine/threonine kinase like (MASTL Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    E167D

    Promoter

    Available since

    Nov. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBabe 3XFLAG-S/T3A-GATA6-3XAU1 puro

    Plasmid

    #72611

    Purpose

    For mammalian expression of an N-terminally triple FLAG-tagged and C-terminally triple AU1-tagged full length human GATA6 with triple alanine mutations on aminoacids#33,#34 and #37

    Depositor

    Kevin Janes

    Article

    Chitforoushzadeh et al Sci Signal. 2016 Jun 7;9(431):ra59. doi:

    Insert

    GATA binding protein 6 (GATA6 Human)
    

    Use

    Retroviral

    Tags

    3XAU1 and 3XFLAG

    Expression

    Mammalian

    Mutation

    changed serine33, threonine34 and serine37 to ala…

    Promoter

    Available since

    Feb. 18, 2016

    Availability

    Academic Institutions and Nonprofits only

13. pTH748-CEN-RLuc/min8maxCFLuc

    Plasmid

    #38214

    Depositor

    Tobias von der Haar

    Article

    Chu et al EMBO J. 2014 Jan 1;33(1):21-34. doi: 10.

    Inserts

    Firefly Luciferase
    Renilla luciferase
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    The first eight codons of the original FLuc gene …

    Promoter

    ADH1 and TDH3 (=GDP)

    Available since

    Nov. 2, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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14. pTH753-CEN-RLuc/min346maxCFLuc

    Plasmid

    #38219

    Depositor

    Tobias von der Haar

    Article

    Chu et al EMBO J. 2014 Jan 1;33(1):21-34. doi: 10.

    Inserts

    Firefly Luciferase
    Renilla luciferase
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    The first 346 codons of the original FLuc gene we…

    Promoter

    ADH1 and TDH3 (=GPD)

    Available since

    Oct. 22, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pTH747-CEN-RLuc/min4maxCFLuc

    Plasmid

    #38213

    Depositor

    Tobias von der Haar

    Article

    Chu et al EMBO J. 2014 Jan 1;33(1):21-34. doi: 10.

    Inserts

    Firefly Luciferase
    Renilla luciferase
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    The first four codons of the original FLuc gene w…

    Promoter

    ADH1 and TDH3 (=GDP)

    Available since

    Oct. 22, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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16. Lenti-LucA

    Plasmid

    #174047

    Purpose

    Expresses mALG8 (ITYTWTRL) antigen as a fusion to luciferase and Cre recombinase

    Depositor

    Tyler Jacks

    Article

    Burger et al Cell. 2021 Sep 16;184(19):4996-5014.e26.

    Insert

    LucA
    

    Use

    Cre/Lox, Lentiviral, and Luciferase

    Tags

    Luciferase

    Expression

    Mammalian

    Mutation

    ALG8 alanine 506 to threonine

    Promoter

    Human Ubiquitin C

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCX-OKS-2A

    Plasmid

    #19771

    Purpose

    Non-integrating (episomal) expression of mouse Oct3/4, Klf4 and Sox2

    Depositor

    Shinya Yamanaka

    Article

    Okita et al Science. 2008 Nov 7. 322(5903):949-53.

    Insert

    Oct3/4-2A-Klf4-2A-Sox2 (Sox2, Klf4, Pou5f1 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Three genes are connected with the foot-and-mouth…

    Promoter

    Available since

    Jan. 5, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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18. pDONR223-STK32C

    Plasmid

    #23720

    Depositor

    David Root, William Hahn

    Article

    Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

    Insert

    STK32C (STK32C Human)
    

    Use

    Gateway donor vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 30, 2010

    Availability

    Academic Institutions and Nonprofits only

19. pC0054-CMV-dPspCas13b-longlinker-ADAR2DD(E488Q/T375G)

    Plasmid

    #103870

    Purpose

    High specificity version of dPspCas13b-ADAR2DD(E488Q) fusions. T375G mutation in the ADAR deaminase domain confers increased specificity with slightly reduced activity.

    Depositor

    Feng Zhang

    Article

    Cox et al Science. 2017 Oct 25. pii: eaaq0180. doi

    Inserts

    PspCas13b
    huADAR2DD (ADARB1 Human)
    

    Use

    CRISPR

    Tags

    GSGGGGS and HIV NES

    Expression

    Mammalian

    Mutation

    Changed histidne 133 to alanine and histidine 105…

    Promoter

    Available since

    Dec. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pHEE2E-TRI

    Plasmid

    #71288

    Purpose

    Egg cell-specific promoter-controlled expression 3×FLAG-NLS-zCas9-NLS and two sgRNAs targeting three genes: ETC2, TRY, and CPC

    Depositor

    Qi-Jun Chen

    Article

    Wang et al Genome Biol. 2015 Jul 21;16:144. doi: 10

    Inserts

    sgRNA targeting TRY and CPC genes
    sgRNA targeting ETC2 gene
    zCas9
    

    Use

    CRISPR; Plant binary vector

    Tags

    3x FLAG and NLS

    Expression

    Plant

    Mutation

    Zea mays codon-optimized Cas9

    Promoter

    EC1.2 enhancer fused to EC1.1 promoter and U6-26p…

    Available since

    Jan. 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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