We narrowed to 10,330 results for: plasmids 101

1. paavCAG-JxON-pre-mGRASP-mCerulean

   Plasmid

   #51903

   Purpose

   JxOn-pre

   Depositor

   Jinhyun Kim

   Article

   Druckmann et al Neuron. 2014 Feb 5;81(3):629-40. doi: 10

   Insert

   presynaptic mGRASP
   

   Use

   AAV and Cre/Lox

   Tags

   Expression

   Mutation

   Promoter

   CAG

   Available Since

   Jan. 27, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pETM6-T7-VHH72

   Plasmid

   #202487

   Purpose

   Expresses the nanobody VHH72 with T7 induction

   Depositor

   Michael Lynch

   Article

   Hennigan et al Metab Eng. 2024 Sep;85:116-130. doi: 10.

   Insert

   nanobody VHH72
   

   Use

   Tags

   6XHis

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Nov. 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSEED-mCherry

   Plasmid

   #219657

   Purpose

   Permits GoldenGate (BsmBI) assembly of donor plasmids to knock-in mCherry using the SEED/Harvest method

   Depositor

   Markus Affolter

   Article

   Aguilar et al Dev Cell. 2024 Oct 7;59(19):2672-2686.e5

   Insert

   sfGFP SEED Cassette
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Oct. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pLminP_Luc2P_RE44

   Plasmid

   #90382

   Purpose

   Hedgehog - Gli1 gene reporter

   Depositor

   Ramnik Xavier

   Article

   O'Connell et al Cell Syst. 2016 May 25;2(5):323-34. doi:

   Insert

   Firefly luciferase
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   RUNX

   Available Since

   Nov. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLminP_Luc2P_RE49

   Plasmid

   #90388

   Purpose

   PPARg/RARa - PPARg gene reporter

   Depositor

   Ramnik Xavier

   Article

   O'Connell et al Cell Syst. 2016 May 25;2(5):323-34. doi:

   Insert

   Firefly luciferase
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   IRF3

   Available Since

   Nov. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-hSyn-ConVERGD-CoffFon-eGFP-W3SL

   Plasmid

   #218750

   Purpose

   Provides NOT intersectional (Flp NOT Cre) expression of eGFP

   Depositor

   Lindsay Schwarz

   Article

   Hughes et al bioRxiv. 2023 Feb 8:2023.02.07.527312. d

   Insert

   eGFP
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   hSyn

   Available Since

   May 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pZac2.1 hSynapsin1 NAPA-N SV40

   Plasmid

   #92282

   Purpose

   FRET probe; extracellular synapse targeted mCherry

   Depositor

   Baljit Khakh

   Article

   Octeau et al Neuron. 2018 Apr 4;98(1):49-66.e9. doi:

   Insert

   Neuron-astrocyte proximity assay - Neuronal
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   hSynapsin1

   Available Since

   April 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. AAV-CAG-LSL-mCherry-shPtbp1

   Plasmid

   #178591

   Purpose

   Cre-dependent expression of mCherry and a shRNA against Ptbp1 under the constitutive promoter

   Depositor

   Chun-Li Zhang

   Article

   Wang et al Cell. 2021 Oct 14;184(21):5465-5481.e16.

   Insert

   miRE-Ptbp1
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Feb. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. PNCA-GFP-dYY1

   Plasmid

   #67791

   Purpose

   Use together with pCMV-intron and VSV-G to package MMLV reporter virus

   Depositor

   Stephen Goff

   Article

   Schlesinger et al Cell Rep. 2013 Jul 11;4(1):50-8. doi: 10

   Insert

   MMLV-LTR-GFP
   

   Use

   Tags

   Expression

   Mutation

   Deletion of LTR U3 YY1 binding domain

   Promoter

   Available Since

   Sept. 1, 2015

   Availability

   Academic Institutions and Nonprofits only

10. pSEED-Dendra2

    Plasmid

    #219645

    Purpose

    Permits GoldenGate assembly (BsmBI) of donor plasmids to knock-in Dendra2 using the SEED/Harvest method

    Depositor

    Markus Affolter

    Article

    Aguilar et al Dev Cell. 2024 Oct 7;59(19):2672-2686.e5

    Insert

    Dendra2 SEED Cassette
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Oct. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pSEED-OLLAS:HA

    Plasmid

    #219647

    Purpose

    Permits GoldenGate assembly (BsmBI) of donor plasmids to knock-in OLLAS and HA tags using the SEED/Harvest method

    Depositor

    Markus Affolter

    Article

    Aguilar et al Dev Cell. 2024 Oct 7;59(19):2672-2686.e5

    Insert

    OLLAS:HA SEED Cassette
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Nov. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. APneg-SNAP25a

    Plasmid

    #168011

    Purpose

    negative selection PACE Accessory Plasmid

    Depositor

    David Liu

    Article

    Blum et al Science. 2021 Feb 19;371(6531):803-810.

    Insert

    SNAP25-T7PAP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    May 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pAB_PalkB_sfgfp_KanR

    Plasmid

    #166503

    Purpose

    Expression of sfGFP under control of PalkB promoter. Sensor output plasmid for detection of n-butanol and branched-chain higher alcohols.

    Depositor

    Sven Panke

    Article

    Bahls et al Metab Eng. 2021 Nov 9. pii: S1096-7176(2

    Insert

    sfGFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PalkB

    Available Since

    Nov. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. TC358

    Plasmid

    #122613

    Purpose

    TRE-delta-pes-10-GFP

    Depositor

    Tian Chi

    Article

    Mao et al iScience. 2019 Jan 25;11:224-237. doi: 1

    Insert

    7XTRE-delta-pes-10-GFP
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available Since

    May 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. AAV-hGFAP-mCherry-shPtbp1

    Plasmid

    #178589

    Purpose

    Expression of mCherry and a shRNA against Ptbp1 under the human GFAP promoter

    Depositor

    Chun-Li Zhang

    Article

    Wang et al Cell. 2021 Oct 14;184(21):5465-5481.e16.

    Insert

    miRE-Ptbp1
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pET26b_Nb.AT110.i1

    Plasmid

    #135481

    Purpose

    E. coli periplasmic expression of high-affinity conformational nanobody Nb.AT110.i1 recognizing angiotensin II type 1 receptor

    Depositor

    Andrew Kruse

    Article

    Wingler et al Cell. 2019 Jan 24;176(3):479-490.e12. do

    Insert

    Nanobody AT110.i1
    

    Use

    Tags

    6xHis-tag and pelB leader

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Aug. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. AP-SNAP25

    Plasmid

    #167995

    Purpose

    positive selection PACE Accessory Plasmid

    Depositor

    David Liu

    Article

    Blum et al Science. 2021 Feb 19;371(6531):803-810.

    Insert

    SNAP25-T7PAP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    April 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. AAV-hGFAP-mCherry-shLuci

    Plasmid

    #178588

    Purpose

    Expression of mCherry and a shRNA against luciferase under the human GFAP promoter

    Depositor

    Chun-Li Zhang

    Article

    Wang et al Cell. 2021 Oct 14;184(21):5465-5481.e16.

    Insert

    miRE-Luciferase
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. AP-VAMP4

    Plasmid

    #168000

    Purpose

    positive selection PACE Accessory Plasmid

    Depositor

    David Liu

    Article

    Blum et al Science. 2021 Feb 19;371(6531):803-810.

    Insert

    VAMP4(I75-L91)-T7PAP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    April 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pZac2.1 hSynapsin1 FLEX NAPA-N SV40

    Plasmid

    #92283

    Purpose

    FRET probe; extracellular synapse targeted mCherry, Cre-dependently expressed

    Depositor

    Baljit Khakh

    Article

    Octeau et al Neuron. 2018 Apr 4;98(1):49-66.e9. doi:

    Insert

    Neuron-astrocyte proximity assay - Neuronal
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    hSynapsin1

    Available Since

    April 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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