We narrowed to 7,350 results for: Lif;

1. plentiCRISPR-sgGOT1

   Plasmid

   #72874

   Purpose

   The lentiviral sgGOT1 vector was generated via ligation of hybridized oligos (below) into lentiCRISPR-v1 vector linearized with BsmBI using Gibson assembly (NEB).

   Depositor

   David Sabatini

   Article

   Birsoy et al Cell. 2015 Jul 30;162(3):540-51. doi: 10

   Insert

   sgGOT1
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 4, 2016

   Availability

   Academic Institutions and Nonprofits only

2. pAV0783

   Plasmid

   #133515

   Purpose

   Expression of mCherry tagged LifeAct probe to monitor actin cytoskeleton

   Depositor

   Sophie Martin

   Article

   Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

   Insert

   pAde6(PmeI)-p(act1)-LifeAct-mCherry-terminator(ScADH1)-bsdMX
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Nov. 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. MSCV KSR1 IRES GFP

   Plasmid

   #25973

   Depositor

   Rob Lewis

   Article

   Kortum et al Mol Cell Biol. 2004 May . 24(10):4407-16

   Insert

   Kinase suppressor of Ras 1 (Ksr1 Mouse)
   

   Use

   Retroviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 24, 2010

   Availability

   Academic Institutions and Nonprofits only

4. pCMV-Sport6 Baf60b

   Plasmid

   #24723

   Depositor

   Benoit Bruneau

   Article

   Lickert et al Nature. 2004 Nov 4. 432(7013):107-12.

   Insert

   Baf60b (Smarcd2 Mouse)
   

   Use

   Tags

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   Available since

   Aug. 2, 2010

   Availability

   Academic Institutions and Nonprofits only

5. pAAV-myrSNAP-CON

   Plasmid

   #60099

   Purpose

   Expresses membrane-targeted SNAP(f)-tag and histone-GFP, conditional

   Depositor

   Gregory Jefferis

   Article

   Kohl et al Proc Natl Acad Sci U S A. 2014 Aug 25. p

   Inserts

   SNAP-tag
   histone-GFP
   

   Use

   AAV and Mouse Targeting

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 26, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMA2867

   Plasmid

   #25439

   Depositor

   Mikhail Alexeyev

   Article

   Alexeyev et al Mol Biol Rep. 2010 Apr . 37(4):1987-91.

   Insert

   mCherry-miR155-lacZalpha (Mir155hg Discosoma-mouse-E. coli, Mouse)
   

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 13, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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7. pT2A-EKAREV-NLS

   Plasmid

   #173856

   Purpose

   Encoding EKAREV-NLS.

   Depositor

   Michiyuki Matsuda

   Article

   Lin et al Life Sci Alliance. 2021 Oct 19;5(1). pii

   Insert

   EKAREV-NLS
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pTYF-2xSYN-tTA

   Plasmid

   #19982

   Depositor

   Sergey Kasparov

   Article

   Liu et al J Gene Med. 2008 May . 10(5):583-92.

   Insert

   2xSYN-tTA (SYN1 Budding Yeast, E.coli, Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 13, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBSc + MShab

   Plasmid

   #16146

   Depositor

   Larry Salkoff

   Article

   Pak et al J Neurosci. 1991 Mar . 11(3):869-80.

   Insert

   Mus musculus potassium voltage gated channel (Kcnb1 Fly, Mouse)
   

   Use

   Xenopus oocyte expression

   Tags

   Expression

   Mutation

   N/A

   Promoter

   Available since

   Jan. 10, 2008

   Availability

   Academic Institutions and Nonprofits only

10. pLV(shRNA)-Puro-U6-CCEPR

    Plasmid

    #108277

    Purpose

    shRNA for CCEPR lncRNA

    Depositor

    Karl Munger

    Article

    Sharma et al Virology. 2018 Feb 7;518:8-13. doi: 10.1

    Insert

    CCEPR (CCEPR Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    April 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMH-HT_CtCHAD

    Plasmid

    #127785

    Purpose

    Expresses CtCHAD in E. coli cells

    Depositor

    Michael Hothorn

    Article

    Lorenzo-Orts et al Life Sci Alliance. 2019 May 27;2(3). pii

    Insert

    CtCHAD (CT0884 Chlorobium tepidum)
    

    Use

    Tags

    6xHis tag + TEV cleavage site

    Expression

    Bacterial

    Mutation

    Residues 208-522 (CHAD domain)

    Promoter

    Available since

    Aug. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pRS315-PGK-SEIPIN1

    Plasmid

    #96986

    Purpose

    Express GFP-tagged Arabidopsis SEIPIN1 gene in yeast cells.

    Depositor

    Kent Chapman

    Article

    Cai et al Plant Cell. 2015 Sep;27(9):2616-36. doi:

    Insert

    SEIPIN1 (AT5G16460 Mustard Weed)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Phosphoglycerate Kinase (PGK) promoter

    Available since

    Aug. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCAGGS-mTagBFP2-T2A-sfGFP-iCre-ERT2

    Plasmid

    #128166

    Purpose

    Monomeric TagBFP2 and sfGFP-iCre-ERT2 recombinase (PAPGSTM N-terminus, unskippable linker)

    Depositor

    Mario Capecchi

    Article

    Nam et al Neural Dev. 2020 Mar 17;15(1):3. doi: 10

    Insert

    mTagBFP2-T2A-sfGFP-iCre-ERT2
    

    Use

    Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. C413-E22: attB4-polyhedrinp>-attB1r

    Plasmid

    #162922

    Purpose

    Gateway attB4/attB1r entry clone for baculovirus expression using the AcMNPV polyhedrin promoter

    Depositor

    Dominic Esposito

    Article

    Wall et al Methods Mol Biol. 2014;1116:193-208. doi

    Insert

    AcMNPV polyhedrin promoter
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMH-HT_SsCHAD

    Plasmid

    #127783

    Purpose

    Expresses SsCHAD in E. coli cells

    Depositor

    Michael Hothorn

    Article

    Lorenzo-Orts et al Life Sci Alliance. 2019 May 27;2(3). pii

    Insert

    SsCHAD (SULM_RS11045 Sulfolobus solfataricus)
    

    Use

    Tags

    6xHis tag + TEV cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pXWJ109As-gfp

    Plasmid

    #123143

    Purpose

    An arsenic sensor with optimized intracellular receptor density. pSB3K3 carrying J109-30arsR-t-ParsR-30gfp-t

    Depositor

    Baojun Wang

    Article

    Wan et al Nat Chem Biol. 2019 May;15(5):540-548. d

    Insert

    J109-30arsR-t-ParsR-30gfp-t
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J109, ParsR

    Available since

    May 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. Rc/CMV cyclin D1 K112E

    Plasmid

    #8951

    Depositor

    Philip Hinds

    Article

    Baker et al Cell Cycle. 2005 Feb . 4(2):330-8.

    Insert

    cyclin D1 (CCND1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    K112E: point mutation in cyclin box

    Promoter

    Available since

    Oct. 6, 2005

    Availability

    Academic Institutions and Nonprofits only

18. pXSE901B

    Plasmid

    #91713

    Purpose

    CRISPR/Cas9-mediated base editing in dicots. Contains Cas9(D10A) under CaMV 35S promoter and empty gRNA scaffold, Bar resistance

    Depositor

    Qi-Jun Chen

    Article

    Chen et al Sci China Life Sci. 2017 Mar 16. doi: 10

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    May 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pSLS.546

    Plasmid

    #184963

    Purpose

    Test effect of a1/a2 length on editing priB using retron recombineering

    Depositor

    Seth Shipman

    Article

    Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

    Insert

    Eco1 RT and recombineering ncRNA, priB G1069A, a1/a2 length: 22
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    priB donor G1069A, a1/a2 length extended to 22 bp

    Promoter

    T7/lac

    Available since

    Nov. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA5/FRT/TO_Lyn11-STf

    Plasmid

    #182008

    Purpose

    CMV overexpression of SNAP-tag fast at the inner leaflet of the plasma membrane of mammalian cell lines

    Depositor

    Kai Johnsson

    Article

    Frei et al ACS Chem Biol. 2022 Jun 17;17(6):1321-13

    Insert

    Lyn11-STf
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Fast mutation on SNAP-tag E30R

    Promoter

    CMV

    Available since

    Nov. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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