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pTCB-Sig9R120AFcholeHA

Plasmid

#237369

Purpose

pTwist CMV BetaGlobin encoding Siglec-9-Fc R120A mutant with hole Fc and HA tag

Depositor

Carolyn Bertozzi, Jessica Stark

Article

Stark et al Nat Biotechnol. 2025 Dec 16. doi: 10.103

Insert

Siglec-9-Fc (R120A mutant) (SIGLEC9 Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pTCB-Sig10FcholeHA

Plasmid

#237370

Purpose

pTwist CMV BetaGlobin encoding Siglec-10-Fc with hole Fc and HA tag

Depositor

Carolyn Bertozzi, Jessica Stark

Article

Stark et al Nat Biotechnol. 2025 Dec 16. doi: 10.103

Insert

Siglec-10-Fc (SIGLEC10 Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pTCB-magroHCLALAPGknob6xHis

Plasmid

#237375

Purpose

pTwist CMV BetaGlobin encoding magrolimab heavy chain with LALA P329G knob Fc and 6xHis tag

Depositor

Carolyn Bertozzi, Jessica Stark

Article

Stark et al Nat Biotechnol. 2025 Dec 16. doi: 10.103

Insert

magrolimab heavy chain (silent Fc)

Tags

6xHis

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pCAG coca-5HT3-IRES-GFP

Plasmid

#242193

Purpose

Expression vector for cocaine-gated chemogenetic cation channel

Depositor

Scott Sternson

Article

Gomez et al Nature. 2025 Aug 27. doi: 10.1038/s41586

Insert

coca-5HT3-IRES-GFP (Htr3a Mouse)

Expression

Mammalian

Mutation

L141G G175K Y210F Y217F

Promoter

CAG

Available Since

Oct. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pUltra-EGFP-P2A-Tr-NRL

Plasmid

#239100

Purpose

pUltra-EGFP bi-cistronic lentiviral vector expressing EGFP and truncated NRL.

Depositor

David Cobrinik

Article

Shayler et al eLife 13:RP101918

Insert

Neural Retina Leucine Zipper (truncated) (NRL Human)

Use

Lentiviral

Available Since

Sept. 17, 2025

Availability

Academic Institutions and Nonprofits only

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pACT-HAB1

Plasmid

#241276

Purpose

Yeast two hybrid vector expressing AD-HAB1

Depositor

Sean Cutler

Article

Park et al Science. 2009 May 22;324(5930):1068-71.

Insert

HAB1 (HAB1 Mustard Weed)

Expression

Yeast

Mutation

NA

Available Since

Sept. 8, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA4-C-EF1α-Tr-NRL

Plasmid

#239096

Purpose

Expresses truncated NRL, driven by EF1α promoter.

Depositor

David Cobrinik

Article

Shayler et al eLife 13:RP101918

Insert

Neural Retina Leucine Zipper (truncated) (NRL Human)

Expression

Mammalian

Available Since

Sept. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA4-C-EF1α-FL-NRL

Plasmid

#239095

Purpose

Expresses full length NRL, driven by EF1α promoter.

Depositor

David Cobrinik

Article

Shayler et al eLife 13:RP101918

Insert

Neural Retina Leucine Zipper (NRL Human)

Expression

Mammalian

Available Since

Sept. 2, 2025

Availability

Academic Institutions and Nonprofits only

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PKT2/CLP-Aoah

Plasmid

#239612

Purpose

Expresses mouse Aoah protein in the transposon vector

Depositor

Xin-yuan Guan

Article

Gong et al Cell. 2025 Jul 24:S0092-8674(25)00793-7.

Insert

Acyloxyacyl Hydrolase (Aoah Mouse)

Use

Transposon

Tags

HA

Expression

Mammalian

Available Since

Aug. 15, 2025

Availability

Academic Institutions and Nonprofits only

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pGEX4T-1 AMPK αKD(27-290aa)

Plasmid

#227853

Purpose

To express in E. coli AMPK αKD(27-290aa)

Depositor

Sheng-cai Lin

Article

Li et al Cell Res. 2024 Jun 19. doi: 10.1038/s414

Insert

AMPK (PRKAA1 Mouse)

Tags

GST

Expression

Bacterial

Mutation

aa 27-290 only

Available Since

July 8, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA 3.3 N-MYC TB182

Plasmid

#227882

Purpose

Used for transfection of cells for expression of C-MYC TB182

Depositor

Sheng-cai Lin

Article

Li et al Cell Res. 2024 Jun 19. doi: 10.1038/s414

Insert

TB182 (TNKS1BP1 Human)

Tags

MYC

Expression

Mammalian

Available Since

July 8, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA 3.3 N-MYC PDHA1

Plasmid

#227877

Purpose

Used for transfection of cells for expression of C-MYC PDHA1

Depositor

Sheng-cai Lin

Article

Li et al Cell Res. 2024 Jun 19. doi: 10.1038/s414

Insert

PDHA1 (PDHA1 Human)

Tags

MYC

Expression

Mammalian

Available Since

July 8, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA 3.3 C-MYC pdzd-8(536A)

Plasmid

#227885

Purpose

Used for transfection of cells for expression of C-MYC pdzd-8(536A)

Depositor

Sheng-cai Lin

Article

Li et al Cell Res. 2024 Jun 19. doi: 10.1038/s414

Insert

pdzd-8 (C53B4.4 Nematode)

Tags

MYC

Expression

Mammalian

Mutation

S536A

Available Since

July 8, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA 3.3 N-MYC pdzd-8

Plasmid

#227844

Purpose

Used for transfection of cells for expression of C-MYC pdzd-8

Depositor

Sheng-cai Lin

Article

Li et al Cell Res. 2024 Jun 19. doi: 10.1038/s414

Insert

pdzd-8 (C53B4.4 Nematode)

Tags

MYC

Expression

Mammalian

Available Since

June 27, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3 Step-FLAG FBXO21

Plasmid

#236458

Purpose

transient overexpression of FBXO21 in mammalian cells

Depositor

Michele Pagano

Article

Donato et al Nat Cell Biol. 2017 Apr;19(4):341-351. d

Insert

FBXO21 (FBXO21 Human)

Tags

Strep-FLAG

Expression

Mammalian

Available Since

May 21, 2025

Availability

Academic Institutions and Nonprofits only

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Str-KDEL_SBP-EGFP-LAP

Plasmid

#222322

Purpose

Synchronize the trafficking of LAP from the ER.

Depositor

Franck Perez

Article

Ecard et al Mol Biol Cell. 2024 Mar 1;35(3):ar42. do

Insert

Streptavidin-KDEL and LAP fused to SBP-EGFP (ACP2 Human)

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 4, 2025

Availability

Academic Institutions and Nonprofits only

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Str-KDEL_SBP-EGFP-EGFR

Plasmid

#222325

Purpose

Synchronize the trafficking of EGFR from the ER.

Depositor

Franck Perez

Article

Scharaw et al J Cell Biol. 2016 Nov 21;215(4):543-558.

Insert

Streptavidin-KDEL and EGFR fused to SBP-EGFP (EGFR Human)

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 4, 2025

Availability

Academic Institutions and Nonprofits only

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DG SaCas9 puro V3

Plasmid

#226960

Purpose

CBh-SaCas9-2A-Puro, and 2X hU6-sgRNA (Sa) with BbsI golden gate cloning backbone for dual gRNAs. sgRNA uses 3TC scaffold for increased sgRNA expression.

Depositor

Paul Thomas

Article

Chey et al BMC Genomics. 2025 Feb 12;26(1):138. doi

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Available Since

Oct. 30, 2024

Availability

Academic Institutions and Nonprofits only

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pHR-dSV40-CD80-mApple

Plasmid

#223595

Purpose

Lentiviral expression of human CD80 with mApple in mammalian cells

Depositor

Enfu Hui

Article

Zhao et al Immunity. 2019 Dec 17;51(6):1059-1073.e9

Insert

CD80 (CD80 Human)

Use

Lentiviral; mammalian expression

Tags

mApple

Available Since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pHR-dSV40-CD80-mGFP

Plasmid

#223592

Purpose

Lentiviral expression of human CD80 with mGFP tag in mammalian cells

Depositor

Enfu Hui

Article

Zhao et al Immunity. 2019 Dec 17;51(6):1059-1073.e9

Insert

CD80 (CD80 Human)

Use

Lentiviral; mammalian expression

Tags

mGFP

Available Since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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