We narrowed to 5,331 results for: pcDNA3.1

1. CMV-CASANOVA

   Plasmid

   #113035

   Purpose

   CASANOVA (AcrIIA4-LOV2 hybrid) expression in mammalian cells; enables optogenetic control of SpyCas9

   Depositor

   Dominik Niopek

   Article

   Bubeck et al Nat Methods. 2018 Oct 30. pii: 10.1038/s

   Insert

   CASANOVA (for CRISPR/Cas9 activity switching via a novel optogenetic variant of AcrIIA4)
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Mammalian

   Available Since

   Oct. 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA-mCherry-TAG-EGFP

   Plasmid

   #232156

   Purpose

   A fluorescence reporter to show incorporation of target unnatural amino acids.

   Depositor

   Nanxi Wang

   Article

   Shao et al Nat Commun. 2025 Jan 8;16(1):227. doi: 1

   Insert

   mCherry-linker(TAG)-EGFP
   

   Use

   Synthetic Biology

   Expression

   Mammalian

   Available Since

   July 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. SacI ML GFP Strand 11 Long

   Plasmid

   #164120

   Purpose

   Detect the long Mitochondria-Endoplasmic reticulum contact along with the OMMGFP1-10

   Depositor

   Tito Calì, Marisa Brini

   Article

   Cieri et al Cell Death Differ. 2018 Jun;25(6):1131-1

   Insert

   SacI ML GFP Strand 11 Long
   

   Expression

   Mammalian

   Available Since

   Jan. 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. Opto-GPR149

   Plasmid

   #106049

   Purpose

   Expression of a chimeric G-protein coupled receptor (Rhodopsin-GPR149; Opto-GPR149; E3)

   Depositor

   Harald Janovjak

   Article

   Morri et al Nat Commun. 2018 May 16;9(1):1950. doi:

   Insert

   Opto-GPR149 (GPR149 Human, Bovine)
   

   Tags

   Rhodopsin-1D4 and VSV-G

   Expression

   Mammalian

   Mutation

   Chimeric receptor protein

   Promoter

   CMV

   Available Since

   Jan. 23, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. SacI ML GFP Strand 11 Short

   Plasmid

   #164121

   Purpose

   Detect the short Mitochondria-Endoplasmic reticulum contact along with the OMMGFP1-10

   Depositor

   Tito Calì, Marisa Brini

   Article

   Cieri et al Cell Death Differ. 2018 Jun;25(6):1131-1

   Insert

   SacI ML GFP Strand 11 Short
   

   Expression

   Mammalian

   Available Since

   Jan. 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. GFP scFv [N86/38]

   Plasmid

   #182095

   Purpose

   Mammalian Expression of GFP scFv. Derived from hybridoma N86/38.

   Depositor

   James Trimmer

   Article

   Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

   Insert

   recombinant mouse scFv targeting GFP
   

   Tags

   HA, His

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. CoV2-Spike-D614G

   Plasmid

   #177960

   Purpose

   Transient mammalian expression of codon optimized SARS-CoV-2 Spike protein (original Wuhan Hu1 sequence + D614G mutation)

   Depositor

   Jennifer Doudna

   Article

   Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

   Insert

   SARS-CoV-2 Spike protein (original Wuhan Hu1 sequence + D614G mutation) (S )
   

   Expression

   Mammalian

   Mutation

   D614G

   Available Since

   Nov. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. Luc-T20

   Plasmid

   #177941

   Purpose

   Transient mammalian expression of Luc2 (firefly luciferase) along with SARS-CoV-2 sequence 20080-22222 (T20) within the 3'UTR. Resulting transcript is packaged into SARS-CoV-2 virus-like particles.

   Depositor

   Jennifer Doudna

   Article

   Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

   Insert

   Luc2 (ORF1ab )
   

   Expression

   Mammalian

   Available Since

   Nov. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA-dCas9-VP64

   Plasmid

   #47107

   Purpose

   Expresses inactivated S. pyogenes dCas9 (D10A, H840A) fused to VP64 transactivator domain in mammalian cells

   Depositor

   Charles Gersbach

   Article

   Perez-Pinera et al Nat Methods. 2013 Jul 25. doi: 10.1038/n

   Insert

   dCas9-VP64
   

   Use

   CRISPR

   Tags

   Flag, HA, SV40 NLS, and VP64

   Expression

   Mammalian

   Mutation

   D10A, H840A (catalytically inactive)

   Promoter

   CMV

   Available Since

   Nov. 14, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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10. hACE2

    Plasmid

    #1786

    Purpose

    Mammalian expression of human ACE2

    Depositor

    Hyeryun Choe

    Article

    Li et al Nature. 2003 Nov 27. 426(6965):450-4.

    Has Service

    Cloning Grade DNA

    Insert

    Angiotensin-converting enzyme 2 (ACE2 Human)
    

    Tags

    C9 (GTETSQVAPA)

    Expression

    Mammalian

    Available Since

    Feb. 14, 2006

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. pcDNA-FLAG-SF2

    Plasmid

    #99021

    Purpose

    Mammalian expression of SF2

    Depositor

    Honglin Chen

    Article

    Huang et al Nat Commun. 2017 Mar 21;8:14751. doi: 10

    Insert

    SF2 (SRSF1 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Available Since

    July 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. opto-E-cad GFP

    Plasmid

    #203327

    Purpose

    Expresses E-cadherin with a LOV2 insert after T133 for blue light inhibition in mammalian cells

    Depositor

    Seraphine Wegner

    Article

    Nzigou Mombo et al Nat Commun. 2023 Oct 9;14(1):6292. doi:

    Insert

    Photoswitchable - E-cadherin with GFP tag (CDH1 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Addition of Aslov2 domain at T133 of human E-cadh…

    Available Since

    April 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. OTR untagged

    Plasmid

    #208913

    Purpose

    encodes the human oxytocin receptor, without any tags

    Depositor

    Christian Gruber

    Article

    Dekan et al Chem Sci. 2021 Feb 4;12(11):4057-4062. d

    Insert

    OXTR (OXTR Human)
    

    Expression

    Mammalian

    Mutation

    TM5: 5x56, A218, Threonin instead of Alanin

    Available Since

    April 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. EphA2-YFP

    Plasmid

    #108852

    Purpose

    Encodes for human EphA2 fluorescently labeled with eYFP on the C-Terminus via a 15 amino acid (GGS)5 flexible linker

    Depositor

    Kalina Hristova

    Article

    Singh et al J Biol Chem. 2015 Nov 6;290(45):27271-9.

    Insert

    EPHA2 (EPHA2 Human)
    

    Tags

    Labeled with eYFP on the C-Terminus via a 15 amin…

    Expression

    Mammalian

    Available Since

    April 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV_OHu19986C_myc tag

    Plasmid

    #183174

    Purpose

    used to make AAV vectors for hsTRPA1 tagged with myc

    Depositor

    Sreekanth Chalasani

    Article

    Duque et al Nat Commun. 2022 Feb 9;13(1):600. doi: 1

    Insert

    hsTRPA1 (TRPA1 Human)
    

    Use

    AAV

    Expression

    Mammalian

    Available Since

    June 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3-FLAG-mTET2

    Plasmid

    #89735

    Purpose

    Expresses wildtype mouse TET2 in mammalian cells

    Depositor

    Yue Xiong

    Article

    Nakagawa et al Mol Cell. 2015 Jan 22;57(2):247-60. doi:

    Insert

    TET2 (Tet2 Mouse)
    

    Tags

    Flag

    Expression

    Mammalian

    Available Since

    May 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. NanoLuc-PM

    Plasmid

    #164784

    Purpose

    Nanoluciferase (NanoLuc) targeted to the extracellular surface of the cell membrane for bioluminescence resonance energy transfer (BRET)-based measurement of ligand binding to unmodified receptors

    Depositor

    Laszlo Hunyady

    Article

    Toth et al J Biol Chem. 2021 Feb 2:100366. doi: 10.

    Insert

    Nanoluciferase fused with the transmembrane domain of the platelet-derived growth factor receptor beta (PDGFRB Human, Oplophorus gracilirostris)
    

    Tags

    NanoLuc

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. NSP6-Flag

    Plasmid

    #210345

    Purpose

    Expresses SARS-CoV-2 NSP6 fused with Flag in mammalian cells

    Depositor

    Michele Pagano

    Article

    Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

    Insert

    SARS-CoV-2 NSP6 (ORF1ab Human, SARS-CoV-2 isolate Wuhan-Hu-1)
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Many synonymous changes due to codon optimization

    Available Since

    Jan. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. CMV-mito-GEM-GECO1

    Plasmid

    #32461

    Purpose

    Mammalian expression of mitochondria-targeting blue-green emission ratiometric genetically encoded Ca2+ indicator for optical imaging

    Depositor

    Robert Campbell

    Article

    Zhao et al Science. 2011 Sep 30;333(6051):1888-91.

    Insert

    GEM-GECO
    

    Tags

    a duplex of the mitochondrial targeting signal of…

    Expression

    Mammalian

    Mutation

    GCaMP3 L60P/K69E/N77Y/D86G/N98I/K119I/L173Q/T223S…

    Promoter

    CMV

    Available Since

    Dec. 5, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3 TAK1/F

    Plasmid

    #44161

    Depositor

    Xin Lin

    Article

    Blonska et al J Biol Chem. 2005 Dec 30;280(52):43056-6

    Insert

    Tak1 (Map3k7 Mouse)
    

    Tags

    Flag

    Expression

    Mammalian

    Available Since

    Oct. 9, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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