We narrowed to 16,423 results for: cript

1. LGhG-pHG165a

   Plasmid

   #90048

   Purpose

   Constitutive expression of a chimeric LacI:GalR transcription repressor

   Depositor

   Liskin Swint-Kruse

   Article

   Tungtur et al J Mol Biol. 2010 Jan 29;395(4):785-802.

   Insert

   LGhG
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Iq

   Available since

   June 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. sgRNA1_pNeurog2-Luciferase reporter

   Plasmid

   #64160

   Purpose

   Photoactivatable transcription system. Mammalian expression of sgRNA1 to target pNeurog2-Luciferase reporter.

   Depositor

   Moritoshi Sato

   Article

   Nihongaki et al Chem Biol. 2015 Feb 19;22(2):169-74. doi

   Insert

   sgRNA1 for pNeurog2-Luciferase reporter
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   human U6

   Available since

   June 23, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pR-Pst

   Plasmid

   #22648

   Depositor

   Lynn Hudson

   Article

   Kim et al J Neurosci Res. 1997 Oct 15. 50(2):272-9

   Insert

   Myelin Transcription Factor 1 (Myt1 Rat)
   

   Use

   Cloning and rna synthesis

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 1, 2009

   Availability

   Academic Institutions and Nonprofits only

4. pCMV Ub Lex VP16 (F442A) HA (P#1711)

   Plasmid

   #14596

   Depositor

   Matija Peterlin

   Article

   Kurosu et al Curr Biol. 2004 Jun 22. 14(12):1112-6.

   Insert

   LexA
   

   Use

   Tags

   HA, Ubiquitin, and VP16 (F442A)

   Expression

   Mammalian

   Mutation

   The VP16 sequence contains an F442A mutation. The…

   Promoter

   Available since

   June 30, 2008

   Availability

   Academic Institutions and Nonprofits only

5. pET-6xHis-SUMO-MarathonRT

   Plasmid

   #109029

   Purpose

   Group II intron maturase from Eubacterium rectale (wild-type sequence). This is a highly processive reverse transcriptase that is called “MarathonRT”

   Depositor

   Anna Pyle

   Article

   Zhao et al RNA. 2018 Feb;24(2):183-195. doi: 10.126

   Insert

   ERE_01490
   

   Use

   Tags

   6xHis-SUMO tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   April 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMW41: pET-His-MBP-SUMO-R2Bm-SII

   Plasmid

   #209290

   Purpose

   Expresses the R2Bm ORF in E. coli

   Depositor

   Feng Zhang

   Article

   Wilkinson et al Science. 2023 Apr 21;380(6642):301-308.

   Insert

   R2
   

   Use

   Tags

   His, MBP, StrepII, and bdSUMO

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Nov. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. VPR-ps-dSpCas9

   Plasmid

   #102855

   Purpose

   A single-chain light-controllable dSpCas9 with pdDronpa1.2 domains

   Depositor

   Michael Lin

   Article

   Zhou et al ACS Chem Biol. 2017 Sep 29. doi: 10.1021

   Insert

   dSpCas9, pdDronpa1.2
   

   Use

   CRISPR and Synthetic Biology

   Tags

   NLS and VP64-p64-Rta transcription activator

   Expression

   Mammalian

   Mutation

   Promoter

   PGK

   Available since

   Nov. 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pQE-WT-RGVG-M6

   Plasmid

   #70172

   Purpose

   Expresses RGVG-M6 T7 RNA polymerase variant with enhanced transcription of 2' modified RNA

   Depositor

   Andrew Ellington

   Article

   Meyer et al Nucleic Acids Res. 2015 Sep 3;43(15):748

   Insert

   RGVG-M6 T7 RNA polymerase
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   T5/Lac

   Available since

   Dec. 16, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. pLTR.gp140/EGFP.Revdelta38/DsRed

   Plasmid

   #115775

   Purpose

   HIV splicing reporter allows the assessment of the effect of LRAs on HIV-1 transcription and splicing.

   Depositor

   Damian Purcell

   Article

   Khoury et al J Vis Exp. 2019 Jan 22;(143). doi: 10.37

   Insert

   Envelope fused to EGFP (gp140-EGFP) and non-functional Rev protein fused to DsRed fluorescent protein (Rev-delta38-DsRed).
   

   Use

   Tags

   EGFP; dsReD

   Expression

   Mammalian

   Mutation

   gp140 uncleaved (1190-91aa, KR > TG; truncated…

   Promoter

   HIV-1 (LTR)

   Available since

   Oct. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMKO.1 puro hTERT shRNA

    Plasmid

    #10688

    Depositor

    William Hahn

    Article

    Masutomi et al Proc Natl Acad Sci U S A. 2005 Jun 7. 10

    Insert

    hTERT shRNA (TERT Human)
    

    Use

    RNAi and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 6, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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11. CROPseq-Puro-24xMS2

    Plasmid

    #212629

    Purpose

    MS2 hairpin-containing expression vector and sgRNA entry plasmid for the directed export of barcode transcripts in Gag-MCP virus-like particles

    Depositor

    Paul Blainey

    Article

    Najia et al bioRxiv 2024.10.01.616098

    Insert

    EF1a-PuroR-24xMS2-WPRE-hU6-sgRNA
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Nov. 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAV126

    Plasmid

    #63192

    Purpose

    pRS426 backbone, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG.

    Depositor

    Jef Boeke

    Article

    Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    Aug. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAV116

    Plasmid

    #63183

    Purpose

    pRS416 backbone, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG.

    Depositor

    Jef Boeke

    Article

    Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    Sept. 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. AAV8-cPE-C

    Plasmid

    #183539

    Purpose

    Deliver a split compact Prime Editor in vivo

    Depositor

    Wen Xue

    Article

    Zheng et al Mol Ther. 2022 Mar 2;30(3):1343-1351. do

    Insert

    C-terminal fragment of SpCas9 nickase (H840A), Reverse Transcriptase
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U1A

    Available since

    June 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAV125

    Plasmid

    #63191

    Purpose

    pRS425 backbone, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG.

    Depositor

    Jef Boeke

    Article

    Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    Oct. 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pGREAT2

    Plasmid

    #170890

    Purpose

    GoldenBraid Double Transcriptional Unit - p35SoTMV_E-Luc_NOSt+pNOS_Red-F_NOSt

    Depositor

    Markita Landry

    Article

    Gonzalez-Grandio et al ACS Synth Biol. 2021 Sep 14. doi: 10.102

    Insert

    E-Luc
    

    Use

    Luciferase and Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    c.375C>T silent mutation to remove BbsI site (…

    Promoter

    p35SoTMV

    Available since

    Oct. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pGREAT1

    Plasmid

    #170889

    Purpose

    GoldenBraid Double Transcriptional Unit - pNOS_E-Luc_NOSt+pNOS_Red-F_NOSt

    Depositor

    Markita Landry

    Article

    Gonzalez-Grandio et al ACS Synth Biol. 2021 Sep 14. doi: 10.102

    Insert

    E-Luc
    

    Use

    Luciferase and Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    c.375C>T silent mutation to remove BbsI site (…

    Promoter

    pNOS

    Available since

    Aug. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pGL2-356bp

    Plasmid

    #16292

    Depositor

    Wafik El-Deiry

    Article

    Wang et al Cancer Res. 2006 Jul 15. 66(14):6982-9.

    Insert

    p53 promoter fragment (TP53 Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    fragment -344 to +12 relative to first transcript…

    Promoter

    Available since

    Dec. 14, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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19. SG3ΔENV RT D185A

    Plasmid

    #145782

    Purpose

    Non-infectious HIV-1 Clone with stop codon in Env (see NIH AIDS Reagent Program Cat #11051) and D185A mutation in reverse transcriptase (RT D185A).

    Depositor

    Wesley Sundquist

    Article

    Christensen et al Science. 2020 Oct 9;370(6513). pii: 370/

    Insert

    Reverse Transcriptase
    

    Use

    Lentiviral and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Changed Reverse Transcriptase Aspartic Acid 185 t…

    Promoter

    Available since

    Oct. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pROSA26-promoter

    Plasmid

    #21710

    Depositor

    Philippe Soriano

    Article

    Zambrowicz et al Proc Natl Acad Sci U S A. 1997 Apr 15. 9

    Insert

    ROSA26 promoter (Gt(ROSA)26Sor Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 28, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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