We narrowed to 13,642 results for: NTS

1. pCW57-CMV-MAAP-SL08

   Plasmid

   #218247

   Purpose

   1) Ectopic expression of MAAP protein variant in mammalian cells. 2) Packaging MAAP variant sequence into lentivirus for stable expression in mammalian cells.

   Depositor

   David Schaffer

   Article

   Schieferecke et al Mol Ther. 2024 Feb 7;32(2):340-351. doi:

   Insert

   Membrane-Associated Accessory Protein Variant SL08 driven by CMV promoter
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   CMV

   Available since

   Oct. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pLX-B3omega1 (GB1694)

   Plasmid

   #160643

   Purpose

   pLX series: pBBR1-based T-DNA binary vector for plant transformation (omega1)

   Depositor

   Fabio Pasin, Juan Antonio García

   Article

   Pasin et al ACS Synth Biol. 2017 Oct 20;6(10):1962-1

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pLV-U6-gRNA-UbC-eGFP-P2A-Bsr

   Plasmid

   #83925

   Purpose

   Lentiviral SpCas9-gRNA expression vector with eGFP-P2A-BlastR

   Depositor

   Charles Gersbach

   Article

   Klann et al Nat Biotechnol. 2017 Apr 3. doi: 10.1038

   Inserts

   eGFP
   Bsr
   

   Use

   CRISPR and Lentiviral

   Tags

   P2A

   Expression

   Mutation

   Promoter

   hUbC

   Available since

   April 6, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pEGFP-C1 RPEL1-EGFP-3XNLS

   Plasmid

   #58469

   Purpose

   Expresses a nuclear-targeted monomeric actin reporter, consisting of the RPEL1 domain from MAL/MRTF, on a CMV promoter

   Depositor

   Dyche Mullins

   Article

   Belin et al Mol Biol Cell. 2013 Apr;24(7):982-94. do

   Inserts

   MRTF-A RPEL1 (Mrtfa Mouse, Synthetic)
   3X NLS with 3X stop codons
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 24, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pENTR-p38KTRmCerulean3

   Plasmid

   #59149

   Purpose

   ORF encoding p38 KTR mCerulean3 flanked by Gateway sequences

   Depositor

   Markus Covert

   Article

   Regot et al Cell. 2014 Jun 19;157(7):1724-34. doi: 1

   Insert

   p38 Kinase Translocation Reporter (MAPK14 Human, Mouse)
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 18, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pMGIB-3xFLAG-pontin

   Plasmid

   #53608

   Purpose

   Retroviral expression of human pontin

   Depositor

   Steven Artandi

   Article

   Venteicher et al Cell. 2008 Mar 21;132(6):945-57. doi: 10

   Insert

   pontin (RUVBL1 Human)
   

   Use

   Retroviral

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   pontin CDS mutated to confer resistance to shRNA.…

   Promoter

   LTR

   Available since

   June 9, 2014

   Availability

   Academic Institutions and Nonprofits only

7. pcDNA3 IKKE K38A flag

   Plasmid

   #26202

   Depositor

   Tom Maniatis

   Article

   Fitzgerald et al Nat Immunol. 2003 May;4(5):491-6.

   Insert

   I-kappa-B kinase epsilon K38A (IKBKE Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Catalytic Lysine changed to Alanine (K38A)

   Promoter

   Available since

   Aug. 24, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pAT9752-BEAR-mScarlet

   Plasmid

   #162991

   Purpose

   BEAR target plasmid with split mScarlet and disrupted 5' splice site

   Depositor

   Ervin Welker

   Article

   Talas et al Nat Commun. 2021 Nov 3;12(1):6353. doi:

   Insert

   mScarlet split with an intron between amino acids 110-111
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   disrupted 5' splice site

   Promoter

   CMV

   Available since

   Sept. 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. p2T-CAG-SpCas9-BlastR

   Plasmid

   #107190

   Purpose

   Confers constitutive expression of SpCas9

   Depositor

   Richard Sherwood

   Article

   Shen et al Nature. 2018 Nov;563(7733):646-651. doi:

   Insert

   SpCas9 (NEWENTRY )
   

   Use

   CRISPR

   Tags

   3xFLAG

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   Chicken ß-actin

   Available since

   Dec. 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pLX-B2 (GB3374)

    Plasmid

    #160636

    Purpose

    pLX series: pBBR1-based T-DNA binary vector for plant transformation (alpha1)

    Depositor

    Fabio Pasin, Juan Antonio García

    Article

    Pasin et al ACS Synth Biol. 2017 Oct 20;6(10):1962-1

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pRK5-FLAG-KPTN

    Plasmid

    #87041

    Purpose

    mammalian expression of KPTN

    Depositor

    David Sabatini

    Article

    Wolfson et al Nature. 2017 Feb 15. doi: 10.1038/nature

    Insert

    KPTN (KPTN Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. CBE-T1.14

    Plasmid

    #193277

    Purpose

    Expression of CBE-T1.14 base editor in mammalian cells, CMV promoter

    Depositor

    Nicole Gaudelli

    Article

    Lam et al Nat Biotechnol. 2023 Jan 9. doi: 10.1038

    Insert

    CBE-T1.14
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    D10A (Cas9), TadA mutations within

    Promoter

    CMV

    Available since

    Jan. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pB[nos-Cas9 PUb-ZsGreen]

    Plasmid

    #169011

    Purpose

    For germline transformation. Expresses Cas9 in germ cells and ZsGreen marker in all cells.

    Depositor

    Max Scott

    Article

    Kandul et al CRISPR J. 2021 Oct;4(5):739-751. doi: 10

    Insert

    Cas9
    

    Use

    Piggybac

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Drosophila melanogaster nanos

    Available since

    April 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pENTR-JNKKTRClover

    Plasmid

    #59139

    Purpose

    ORF encoding JNK KTR mClover flanked by Gateway sequences

    Depositor

    Markus Covert

    Article

    Regot et al Cell. 2014 Jun 19;157(7):1724-34. doi: 1

    Insert

    JNK Kinase Translocation Reporter (MAPK8 Human, Mouse)
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 15, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pEJS887_pTetR-P2A-BFPnls/sgAlpha

    Plasmid

    #108651

    Purpose

    Alpha satellite repeats targeting Spy sgRNA under U6 promoter

    Depositor

    Erik Sontheimer

    Article

    Gao et al Nat Methods. 2018 Jun;15(6):433-436. doi

    Inserts

    Alpha satellite repeats targeting sgRNA
    TetR-P2A-BFP
    

    Use

    CRISPR and Lentiviral

    Tags

    No

    Expression

    Mammalian

    Mutation

    Promoter

    U6 and hPGK

    Available since

    May 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pcDNA5-FRT/TO-N-mEGFP-PARP6

    Plasmid

    #178005

    Purpose

    Expresses N-terminally mEGFP-tagged PARP6

    Depositor

    Karla Feijs

    Article

    Weixler et al Life Sci Alliance. 2022 Nov 11;6(1):e202

    Insert

    PARP6 (PARP6 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pcDNA5-FRT/TO-N-mEGFP-PARP15

    Plasmid

    #178000

    Purpose

    Expresses N-terminally mEGFP-tagged PARP15

    Depositor

    Karla Feijs

    Article

    Weixler et al Life Sci Alliance. 2022 Nov 11;6(1):e202

    Insert

    PARP15 (PARP15 Human)
    

    Use

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pC-HYG-YR

    Plasmid

    #61765

    Purpose

    Yeast recombinational cloning compatible Agrobacterium tumefaciens ternary vector containing hygromycin selectable marker on transfer DNA (TDNA).

    Depositor

    Ken Haynes

    Article

    Sidhu et al Fungal Genet Biol. 2015 Jun;79:102-9. do

    Inserts

    Hygromycin
    2 micron origin or replication and URA3 gene for S. cerevisiae
    

    Use

    Ternary vector for agrobacterium mediated transfo…

    Tags

    Expression

    Mutation

    Promoter

    trpC promoter

    Available since

    May 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. POLArISact

    Plasmid

    #164971

    Purpose

    Expresses POLArISact, an orientation probe that specifically binds to F-actin, in mammalian cells

    Depositor

    Sumio Terada

    Article

    Sugizaki et al Proc Natl Acad Sci U S A. 2021 Mar 16;11

    Insert

    POLArISact
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. MSCV Myc IRES GFP

    Plasmid

    #18770

    Depositor

    Scott Lowe

    Article

    Hemann et al Nature. 2005 Aug 11. 436(7052):807-11.

    Insert

    Myc (Myc Mouse)
    

    Use

    Retroviral

    Tags

    IRES GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 3, 2008

    Availability

    Academic Institutions and Nonprofits only

21. pMX GFP Rac G12V

    Plasmid

    #14567

    Depositor

    Joan Brugge

    Article

    Hall et al Immunity. 2006 Mar . 24(3):305-16.

    Insert

    Rac1 G12V (RAC1 Human)
    

    Use

    Retroviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    G12V

    Promoter

    Available since

    April 12, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. xCas9

    Plasmid

    #138565

    Purpose

    Expresses human codon-optimized expanded PAM SpCas9 variant and blasticidin resistance: EFS promoter-xCas9-NLS-FLAG-P2A-BSD

    Depositor

    Hyongbum Kim

    Article

    Kim et al Nat Biotechnol. 2020 Nov;38(11):1328-133

    Insert

    xCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    BSD, FLAG, and NLS

    Expression

    Mammalian

    Mutation

    A262T, R324L, S409I, E480K, E543D, M694I, E1219V

    Promoter

    EFS

    Available since

    June 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pAAV-hSyn-hPOMC1-26-sbGLuc-P2A-dTomato

    Plasmid

    #176704

    Purpose

    neural activity dependent release of sbGLuc from presynaptic vesicles

    Depositor

    Ute Hochgeschwender

    Article

    Prakash et al Commun Biol. 2022 Jan 11;5(1):33. doi: 1

    Inserts

    Gaussia luciferase, slow burn
    dTomato
    

    Use

    AAV

    Tags

    P2A and hPOMC1-26

    Expression

    Mutation

    Promoter

    hSyn

    Available since

    Nov. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. AM77_pU6.Sa-gRNA.CLYBL

    Plasmid

    #199237

    Purpose

    Expression construct encoding a S. aureus guide RNA targeting the human CLYBL safe harbor locus

    Depositor

    Manuel Goncalves

    Article

    Wang et al Nucleic Acids Res. 2023 Mar 17:gkad165.

    Insert

    S. aureus gRNA spacer
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    RNA polymerase III promoter for human U6 snRNA

    Available since

    April 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pCEP4-myc-HLA-A1

    Plasmid

    #135503

    Purpose

    Mammalian expression of myc-tagged HLA-A*01:01

    Depositor

    Erik Procko

    Article

    McShan et al Proc Natl Acad Sci U S A. 2019 Dec 17;11

    Insert

    HLA-A*01:01 (HLA-A Human)
    

    Use

    Tags

    Extracellular c-myc epitope tag and Signal peptid…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pCD564

    Plasmid

    #153026

    Purpose

    dxCas9(3.7), MCP-SoxS(R93A/S101A)

    Depositor

    Jesse Zalatan

    Article

    Fontana et al Nat Commun. 2020 Apr 1;11(1):1618. doi:

    Insert

    dxCas9(3.7), MCP-SoxS(R93A/S101A)
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    SoxS has R93A and S101A mutations

    Promoter

    Available since

    Aug. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pX330.Pten.b

    Plasmid

    #66588

    Purpose

    sgRNA for Pten deletion

    Depositor

    Wen Xue

    Article

    Li et al Genome Biol. 2015 May 28;16(1):111.

    Insert

    Pten deletion (Pten Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 27, 2015

    Availability

    Academic Institutions and Nonprofits only

28. CoV2-N-P.1

    Plasmid

    #177959

    Purpose

    Transient mammalian expression of codon optimized SARS-CoV-2 Nucleocapsid (P80R, R203K, G204R). Used for generating RNA packaging virus-like particles.

    Depositor

    Jennifer Doudna

    Article

    Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

    Insert

    SARS-CoV-2 Nucleocapsid (P80R, R203K, G204R) (N )
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    P80R,R203K,G204R

    Promoter

    Available since

    Nov. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pLX-Z4 (GB3375)

    Plasmid

    #160648

    Purpose

    pLX series: RK2-based T-DNA binary vector for plant transformation (alpha1)

    Depositor

    Fabio Pasin, Juan Antonio García

    Article

    Pasin et al ACS Synth Biol. 2017 Oct 20;6(10):1962-1

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. px459-TDP 43 sgRNA2

    Plasmid

    #133763

    Purpose

    Expresses Cas9 and human TDP-43 sgRNA

    Depositor

    Shawn Ferguson

    Article

    Roczniak-Ferguson et al Life Sci Alliance. 2019 Sep 16;2(5). pii

    Insert

    TDP-43 (TARDBP Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Jan. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge