We narrowed to 164,273 results for: HTT

1. pLenti-CMV-GFP-miRE_shRen-Blast

   Plasmid

   #135684

   Purpose

   Constitutive expression (CMV promoter) of GFP cDNA plus miRE-embedded shRenilla (control shRNA), Blasticidin selection

   Depositor

   Florian Karreth

   Insert

   shRenilla
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   March 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLenti-CMV-tdTomato-miRE_shRen-Blast

   Plasmid

   #135686

   Purpose

   Constitutive expression (CMV promoter) of tdTomato cDNA plus miRE-embedded shRenilla (control shRNA), Blasticidin selection

   Depositor

   Florian Karreth

   Insert

   shRenilla
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   March 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. mOrange2-CytERM-N-17

   Plasmid

   #57952

   Purpose

   Localization: Endoplasmic Reticulum, Excitation: 549, Emission: 565

   Depositor

   Michael Davidson

   Insert

   CytERM (AA1-29)
   

   Use

   Tags

   mOrange2

   Expression

   Mammalian

   Mutation

   Only first 29 AA of CytERM with mutations V8G, H2…

   Promoter

   CMV

   Available Since

   Oct. 24, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEGB3_alpha1 Pnos:P19:Tnos (GB2762)

   Plasmid

   #160632

   Purpose

   TU for expression of P19 driven by Nos promoter.

   Depositor

   Diego Orzaez

   Insert

   P19
   

   Use

   Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   BsaI and BsmBI sites removed

   Promoter

   Pnos

   Available Since

   Jan. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET His6 High pI protein 69 TEV LIC cloning vector (2-HpI-69)

   Plasmid

   #55212

   Depositor

   Scott Gradia

   Type

   Empty backbone

   Use

   Tags

   6xHis, High pI protein 69, and TEV cleavage site

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   June 16, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. UC20m

   Plasmid

   #121040

   Purpose

   MoClo golden gate assembly DE part for gN2 gRNA (guide RNA for S. pyogenes Cas9; sequence designed with NUPACK for stable tracrRNA structure and open targeting region).

   Depositor

   Richard Murray

   Insert

   gN2 Cas9 gRNA UC part
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Dec. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCAGGS-mTagBFP2-T2A-iCreERT2 (PAA)

   Plasmid

   #137859

   Purpose

   Monomeric TagBFP2 and iCre-ERT2 recombinase (PAA N-terminus)

   Depositor

   Mario Capecchi

   Insert

   mTagBFP2-T2A-iCreERT2
   

   Use

   Cre/Lox

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   March 31, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. R777-E133 Hs.MLST8

   Plasmid

   #70417

   Purpose

   Gateway ORF clone of human MLST8 [NM_022372.4] with stop codon (for native or N-terminal fusions)

   Depositor

   Dominic Esposito

   Insert

   MLST8 (MLST8 Human)
   

   Use

   Gateway entry clone

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   March 1, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. TARS_pNIC-Bio3

   Plasmid

   #153057

   Purpose

   Expression construct for producing Avi tagged Threonyl-tRNA synthetase

   Depositor

   Susanne Gräslund

   Insert

   TARS (TARS1 Human)
   

   Use

   Tags

   Avi and His6-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   June 29, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pAS1-tax-4P::his-24::mOrange

    Plasmid

    #124340

    Purpose

    Multicolor Worms for Neuronal ID

    Depositor

    Andrew Leifer

    Insert

    tax-4, mOrange (tax-4 Nematode)
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available Since

    April 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pE-SUMO CRYGB

    Plasmid

    #80751

    Purpose

    Expression of human gamma B-crystallin in E. coli

    Depositor

    Larry David

    Insert

    CRYGB (CRYGB Human)
    

    Use

    Tags

    6xHIS and Smt3 SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    March 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pBS chick LH-2A [TJ#93]

    Plasmid

    #16280

    Depositor

    Thomas Jessell

    Insert

    LH-2A (LHX2 Chicken)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Feb. 4, 2009

    Availability

    Academic Institutions and Nonprofits only

13. pSpy

    Plasmid

    #29588

    Depositor

    Marc Fiedler

    Insert

    Spy (spy E.coli BL21(DE3))
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    processed form of Spy starting at residue 24 (Rem…

    Promoter

    Available Since

    April 28, 2011

    Availability

    Academic Institutions and Nonprofits only

14. mGeos2-VEL-Lifeact-7

    Plasmid

    #54817

    Purpose

    Localization: Actin, Excitation: 503 / 569, Emission: 514 / 581

    Depositor

    Michael Davidson

    Type

    Empty backbone

    Use

    Tags

    mGeos2-VEL-Lifeact

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 20, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

15. R777-E201 Hs.PRKAG2

    Plasmid

    #70485

    Purpose

    Gateway ORF clone of human PRKAG2 [NM_024429.1] with stop codon (for native or N-terminal fusions)

    Depositor

    Dominic Esposito

    Insert

    PRKAG2 (PRKAG2 Human)
    

    Use

    Gateway entry clone

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    March 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. L4752

    Plasmid

    #1675

    Depositor

    Andrew Fire

    Type

    Empty backbone

    Use

    Tags

    IVSA, pes10UAS-270, pes10Tsa, T7p, gfpN, IVSR, gf…

    Expression

    Worm

    Mutation

    Promoter

    Available Since

    March 7, 2005

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. Mouse NCKX2-AS-long (pcDNA3.1+)

    Plasmid

    #75199

    Purpose

    Mammalian expression of SLC24A2

    Depositor

    Jonathan Lytton

    Insert

    SLC24A2 (Slc24a2 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    alternatively spliced exon present

    Promoter

    CMV

    Available Since

    June 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. T12m

    Plasmid

    #121031

    Purpose

    MoClo golden gate assembly DE part ECK120029600 (378x-strength natural terminator; see DOI: 10.1038/nmeth.2515). Please see Supplemental Documents for annotated Genbank file.

    Depositor

    Richard Murray

    Insert

    ECK120029600 (strongest natural Voigt terminator)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Dec. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. mEos-A69V-Paxillin-22

    Plasmid

    #57338

    Purpose

    Localization: Focal Contacts, Excitation: 505 / 569, Emission: 516 / 581

    Depositor

    Michael Davidson

    Insert

    Paxillin
    

    Use

    Tags

    mEos A69V

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Jan. 29, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

20. R777-E251 Hs.RASSF1

    Plasmid

    #70535

    Purpose

    Gateway ORF clone of human RASSF1 [NM_007182.4] with stop codon (for native or N-terminal fusions)

    Depositor

    Dominic Esposito

    Insert

    RASSF1 (RASSF1 Human)
    

    Use

    Gateway entry clone

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    March 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. MTMR6A

    Plasmid

    #42483

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    MTMR6A
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    See Comments

    Promoter

    Available Since

    March 18, 2013

    Availability

    Academic Institutions and Nonprofits only

22. mApple-Moesin-C-14

    Plasmid

    #54927

    Purpose

    Localization: Cytoskeleton/Membrane, Excitation: 568, Emission: 592

    Depositor

    Michael Davidson

    Insert

    Moesin (MSN Human)
    

    Use

    Tags

    mApple

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Aug. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

23. mPA-GFP-C-Src-7

    Plasmid

    #57127

    Purpose

    Localization: Membrane/Vesicles, Excitation: 400 / 504, Emission: 515 / 517

    Depositor

    Michael Davidson

    Insert

    C-Src
    

    Use

    Tags

    mPA-GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Jan. 29, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

24. mVenus-Zyxin-6

    Plasmid

    #56626

    Purpose

    Localization: Focal Adhesions, Excitation: 515, Emission: 528

    Depositor

    Michael Davidson

    Insert

    Zyxin (ZYX Human)
    

    Use

    Tags

    mVenus

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Dec. 18, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

25. PS-CFP2-MCPA2-N-18

    Plasmid

    #57235

    Purpose

    Localization: Actin Capping Protein , Excitation: 400 / 490, Emission: 468 / 511

    Depositor

    Michael Davidson

    Insert

    MCPA2
    

    Use

    Tags

    PS-CFP2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Nov. 13, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

26. INPP5EA

    Plasmid

    #42481

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    INPP5EA
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    See Comments

    Promoter

    Available Since

    March 18, 2013

    Availability

    Academic Institutions and Nonprofits only

27. R777-E196 Hs.PRKAB1-nostop

    Plasmid

    #70480

    Purpose

    Gateway ORF clone of human PRKAB1 [NM_006253.4] without stop codon (for C-terminal fusions)

    Depositor

    Dominic Esposito

    Insert

    PRKAB1 (PRKAB1 Human)
    

    Use

    Gateway entry clone

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    March 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. R777-E266 Hs.RASSF7-nostop

    Plasmid

    #70550

    Purpose

    Gateway ORF clone of human RASSF7 [NM_001143993.1] without stop codon (for C-terminal fusions)

    Depositor

    Dominic Esposito

    Insert

    RASSF7 (RASSF7 Human)
    

    Use

    Gateway entry clone

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    March 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. R777-E304 Hs.SAV1-nostop

    Plasmid

    #70588

    Purpose

    Gateway ORF clone of human SAV1 [NM_021818.3] without stop codon (for C-terminal fusions)

    Depositor

    Dominic Esposito

    Insert

    SAV1 (SAV1 Human)
    

    Use

    Gateway entry clone

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    March 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. R777-E188 Hs.PPP1CA-nostop

    Plasmid

    #70472

    Purpose

    Gateway ORF clone of human PPP1CA [NM_002708.3] without stop codon (for C-terminal fusions)

    Depositor

    Dominic Esposito

    Insert

    PPP1CA (PPP1CA Human)
    

    Use

    Gateway entry clone

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    March 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pCB94

    Plasmid

    #69743

    Purpose

    Retroviral expression of PP2A beta

    Depositor

    Iain Cheeseman

    Insert

    PP2A beta (PTPA Human)
    

    Use

    Retroviral

    Tags

    eGFP-TEV-S

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Dec. 3, 2015

    Availability

    Academic Institutions and Nonprofits only

32. L4664

    Plasmid

    #1666

    Depositor

    Andrew Fire

    Type

    Empty backbone

    Use

    Tags

    myo3-2341, myo3Tsa, IVSA, pat3MbLS, gfpN, IVSR, g…

    Expression

    Worm

    Mutation

    Promoter

    Available Since

    March 7, 2005

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. SP65U1-301a

    Plasmid

    #105821

    Purpose

    plasmid for the overexpression of miR-301a under the U1 expression cassette inserted in the pSP65 plasmid

    Depositor

    Cecilia Mannironi

    Insert

    MIRNA301 (Mir301 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U1

    Available Since

    Jan. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pJK365

    Plasmid

    #72404

    Purpose

    Produces Acetobacter aceti 1023 Est01

    Depositor

    T. J. Kappock

    Insert

    acyl-CoA thioester hydrolase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Feb. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. 4I79

    Plasmid

    #51319

    Depositor

    Cheryl Arrowsmith

    Insert

    4I79 (NUP43 Human)
    

    Use

    Baculovirus expression

    Tags

    His

    Expression

    Mutation

    Truncation: See Comments

    Promoter

    T7

    Available Since

    Feb. 24, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

36. mEos-A69V-N1

    Plasmid

    #54754

    Purpose

    Localization: N1 Cloning Vector, Excitation: 505 / 569, Emission: 516 / 581

    Depositor

    Michael Davidson

    Type

    Empty backbone

    Use

    Tags

    mEos A69V, V123T, T158H

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 11, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

37. pHH0103 WBP4 WW domain #2

    Plasmid

    #104253

    Purpose

    Bacterial expression of WW domain #2 from WBP4

    Depositor

    Marius Sudol, Sachdev Sidhu

    Insert

    WBP4 WW domain #2 (WBP4 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    codon optimized for expression in bacteria

    Promoter

    tac

    Available Since

    Feb. 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. (P79) OCN/Wnt 10b transgene

    Plasmid

    #25878

    Depositor

    Ormond MacDougald

    Insert

    Wnt10b (Wnt10b Mouse)
    

    Use

    Mouse Targeting

    Tags

    B-globin intron/poly A coding sequence and OCN pr…

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 21, 2010

    Availability

    Academic Institutions and Nonprofits only

39. pE-SUMO CRYGC

    Plasmid

    #80754

    Purpose

    Expression of human gamma C-crystallin in E. coli

    Depositor

    Larry David

    Insert

    CRYGC (CRYGC Human)
    

    Use

    Tags

    6xHIS and Smt3 SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    March 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. R777-E232 Hs.RASA3-nostop

    Plasmid

    #70516

    Purpose

    Gateway ORF clone of human RASA3 [NM_007368.2] without stop codon (for C-terminal fusions)

    Depositor

    Dominic Esposito

    Insert

    RASA3 (RASA3 Human)
    

    Use

    Gateway entry clone

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    March 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge