We narrowed to 17,203 results for: puro

1. pPB-cT3G-cERP2-STRA8

   Plasmid

   #222564

   Purpose

   PiggyBac transposon plasmid for doxycycline inducible expression of STRA8

   Depositor

   George Church

   Article

   Pierson Smela et al bioRxiv 2024.05.31.596483

   Insert

   STRA8 (STRA8 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. LT3GEPIR-CALM1shRNA1

   Plasmid

   #220567

   Purpose

   To inducibly knockdown CALM1 expression

   Depositor

   Sean Lee

   Insert

   CALM1 shRNA1 (CALM1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   TRE3G (TetOP) promoter

   Available since

   July 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. LT3GEPIR-CALM1shRNA2

   Plasmid

   #220568

   Purpose

   To inducibly knockdown CALM1 expression

   Depositor

   Sean Lee

   Insert

   CALM1 shRNA2 (CALM1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   TRE3G (TetOP) promoter

   Available since

   July 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pLX-EF1a_NKX2-4

   Plasmid

   #221490

   Purpose

   NKX2-4 lentiviral overexpression

   Depositor

   Matthew Meyerson

   Article

   Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

   Insert

   NKX2-4 (NKX2-4 Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   July 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. msPlekha8 (msFapp2) g1 lentiCRISPRv2-opti

   Plasmid

   #218650

   Purpose

   Knockout vector for mouse Plekha8 (Fapp2)

   Depositor

   Wade Harper

   Article

   Hollingsworth et al bioRxiv 2024.04.19.590338

   Insert

   Plekha8 (Plekha8 Mouse)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. PLEKHA8 (FAPP2) g2 lentiCRISPRv2-opti

   Plasmid

   #218659

   Purpose

   Knockout vector for human PLEKHA8 (FAPP2)

   Depositor

   Wade Harper

   Article

   Hollingsworth et al bioRxiv 2024.04.19.590338

   Insert

   PLEKHA8 (PLEKHA8 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. msPlekha3 (msFapp1) g2 lentiCRISPRv2-opti

   Plasmid

   #218649

   Purpose

   Knockout vector for mouse Plekha3 (Fapp1)

   Depositor

   Wade Harper

   Article

   Hollingsworth et al bioRxiv 2024.04.19.590338

   Insert

   Plekha3 (Plekha3 Mouse)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. msPlekha3 (msFapp1) g1 lentiCRISPRv2-opti

   Plasmid

   #218648

   Purpose

   Knockout vector for mouse Plekha3 (Fapp1)

   Depositor

   Wade Harper

   Article

   Hollingsworth et al bioRxiv 2024.04.19.590338

   Insert

   Plekha3 (Plekha3 Mouse)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. msPlekha8 (msFapp2) g2 lentiCRISPRv2-opti

   Plasmid

   #218651

   Purpose

   Knockout vector for mouse Plekha8 (Fapp2)

   Depositor

   Wade Harper

   Article

   Hollingsworth et al bioRxiv 2024.04.19.590338

   Insert

   Plekha8 (Plekha8 Mouse)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. PLEKHA3 (FAPP1) g2 lentiCRISPRv2-opti

    Plasmid

    #218657

    Purpose

    Knockout vector for human PLEKHA3 (FAPP1)

    Depositor

    Wade Harper

    Article

    Hollingsworth et al bioRxiv 2024.04.19.590338

    Insert

    PLEKHA3 (PLEKHA3 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. PLEKHA8 (FAPP2) g1 lentiCRISPRv2-opti

    Plasmid

    #218658

    Purpose

    Knockout vector for human PLEKHA8 (FAPP2)

    Depositor

    Wade Harper

    Article

    Hollingsworth et al bioRxiv 2024.04.19.590338

    Insert

    PLEKHA8 (PLEKHA8 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. PLEKHA3 (FAPP1) g1 lentiCRISPRv2-opti

    Plasmid

    #218656

    Purpose

    Knockout vector for human PLEKHA3 (FAPP1)

    Depositor

    Wade Harper

    Article

    Hollingsworth et al bioRxiv 2024.04.19.590338

    Insert

    PLEKHA3 (PLEKHA3 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. p663-UBC-MTS-miniTurbo-V5_IDG-K

    Plasmid

    #214456

    Purpose

    Gateway destination clone of MTS-miniTurbo (mitochondrial control) tagged with C-terminal V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

    Depositor

    Ben Major

    Insert

    MTS-miniTurbo-V5
    

    Use

    Lentiviral; Gateway destination

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Ubiquitin

    Available since

    Feb. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. p663-UBC-MTS-TurboID-V5_IDG-K

    Plasmid

    #214457

    Purpose

    Gateway destination clone of MTS-TurboID (mitochondrial control) tagged with C-terminal V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

    Depositor

    Ben Major

    Insert

    MTS-TurboID-V5
    

    Use

    Lentiviral; Gateway destination

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Ubiquitin

    Available since

    Feb. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. MSCV-P C-FlagHA HPV16 E7 Mutant 2

    Plasmid

    #191546

    Purpose

    Protein expression of HPV16 E7 Mutant 2

    Depositor

    Elizabeth White

    Article

    Nouel et al mBio. 2022 Nov 8:e0203322. doi: 10.1128/

    Insert

    HPV16
    

    Use

    Retroviral

    Tags

    FlagHA

    Expression

    Mutation

    I93A, K97A, P98A

    Promoter

    Available since

    Nov. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. MSCV-P C-FlagHA HPV16-18 E7 Chimera B

    Plasmid

    #191543

    Purpose

    Protein expression of HPV16 E7 Chimera B

    Depositor

    Elizabeth White

    Article

    Nouel et al mBio. 2022 Nov 8:e0203322. doi: 10.1128/

    Insert

    HPV16-18 E7
    

    Use

    Retroviral

    Tags

    FlagHA

    Expression

    Mutation

    CR1 CR2 HPV18 E7, C-terminus HPV16 E7

    Promoter

    Available since

    Nov. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. MSCV-P C-FlagHA HPV16-18 E7 Chimera A

    Plasmid

    #191542

    Purpose

    Protein expression of HPV16 E7 Chimera A

    Depositor

    Elizabeth White

    Article

    Nouel et al mBio. 2022 Nov 8:e0203322. doi: 10.1128/

    Insert

    HPV16-18 E7
    

    Use

    Retroviral

    Tags

    FlagHA

    Expression

    Mutation

    CR1 CR2 HPV16 E7, C-terminus HPV18 E7

    Promoter

    Available since

    Nov. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. MSCV-P C-FlagHA HPV16-18 E7 Chimera C

    Plasmid

    #191544

    Purpose

    Protein expression of HPV16 E7 Chimera C

    Depositor

    Elizabeth White

    Article

    Nouel et al mBio. 2022 Nov 8:e0203322. doi: 10.1128/

    Insert

    HPV16-18 E7
    

    Use

    Retroviral

    Tags

    FlagHA

    Expression

    Mutation

    CR1 HPV18 E7, CR2 C-terminus HPV16 E7

    Promoter

    Available since

    Nov. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. MSCV-P C-FlagHA HPV16 E7 Mutant 1

    Plasmid

    #191545

    Purpose

    Protein expression of HPV16 E7 Mutant 1

    Depositor

    Elizabeth White

    Article

    Nouel et al mBio. 2022 Nov 8:e0203322. doi: 10.1128/

    Insert

    HPV16
    

    Use

    Retroviral

    Tags

    FlagHA

    Expression

    Mutation

    R49A, H51A, H73A, E80A, D81A

    Promoter

    Available since

    Nov. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. MSCV-P C-FlagHA HPV16 E7 E80A/D81A

    Plasmid

    #191548

    Purpose

    Protein expression of HPV16 E7 E80A/D81A

    Depositor

    Elizabeth White

    Article

    Nouel et al mBio. 2022 Nov 8:e0203322. doi: 10.1128/

    Insert

    HPV16
    

    Use

    Retroviral

    Tags

    FlagHA

    Expression

    Mutation

    E80A/D81A

    Promoter

    Available since

    Nov. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pMta2-EosHalo-TAFiP

    Plasmid

    #140959

    Purpose

    Targeting construct to make Mta2-Eos-Halo-Tev-Avi-3xFlag fusion

    Depositor

    Brian Hendrich

    Insert

    metastasis-associated gene family, member 2 (Mta2 Mouse, Synthetic)
    

    Use

    Mouse Targeting

    Tags

    3xFLAG, Avi, Eos, Halo, and TEV

    Expression

    Mutation

    Promoter

    Available since

    Oct. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. CRISPRi-CENPB-g4

    Plasmid

    #120212

    Purpose

    CENPB CRISPRi guide RNA 4

    Depositor

    David Bartel

    Article

    Wu et al Cell. 2017 May 18;169(5):905-917.e11. do

    Insert

    CENPB CRISPRi guide RNA
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. CRISPRi-CENPB-g3

    Plasmid

    #120211

    Purpose

    CENPB CRISPRi guide RNA 3

    Depositor

    David Bartel

    Article

    Wu et al Cell. 2017 May 18;169(5):905-917.e11. do

    Insert

    CENPB CRISPRi guide RNA
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. CRISPRi-CENPB-g10

    Plasmid

    #120218

    Purpose

    CENPB CRISPRi guide RNA 10

    Depositor

    David Bartel

    Article

    Wu et al Cell. 2017 May 18;169(5):905-917.e11. do

    Insert

    CENPB CRISPRi guide RNA
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. CRISPRi-CENPB-g9

    Plasmid

    #120217

    Purpose

    CENPB CRISPRi guide RNA 9

    Depositor

    David Bartel

    Article

    Wu et al Cell. 2017 May 18;169(5):905-917.e11. do

    Insert

    CENPB CRISPRi guide RNA
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. CRISPRi-CENPB-g8

    Plasmid

    #120216

    Purpose

    CENPB CRISPRi guide RNA 8

    Depositor

    David Bartel

    Article

    Wu et al Cell. 2017 May 18;169(5):905-917.e11. do

    Insert

    CENPB CRISPRi guide RNA
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. CRISPRi-CENPB-g7

    Plasmid

    #120215

    Purpose

    CENPB CRISPRi guide RNA 7

    Depositor

    David Bartel

    Article

    Wu et al Cell. 2017 May 18;169(5):905-917.e11. do

    Insert

    CENPB CRISPRi guide RNA
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. CRISPRi-CENPB-g6

    Plasmid

    #120214

    Purpose

    CENPB CRISPRi guide RNA 6

    Depositor

    David Bartel

    Article

    Wu et al Cell. 2017 May 18;169(5):905-917.e11. do

    Insert

    CENPB CRISPRi guide RNA
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. CRISPRi-CENPB-g5

    Plasmid

    #120213

    Purpose

    CENPB CRISPRi guide RNA 5

    Depositor

    David Bartel

    Article

    Wu et al Cell. 2017 May 18;169(5):905-917.e11. do

    Insert

    CENPB CRISPRi guide RNA
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. CRISPRi-CENPB-g2

    Plasmid

    #120210

    Purpose

    CENPB CRISPRi guide RNA 2

    Depositor

    David Bartel

    Article

    Wu et al Cell. 2017 May 18;169(5):905-917.e11. do

    Insert

    CENPB CRISPRi guide RNA
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. CRISPRi-CENPB-g1

    Plasmid

    #120209

    Purpose

    CENPB CRISPRi guide RNA 1

    Depositor

    David Bartel

    Article

    Wu et al Cell. 2017 May 18;169(5):905-917.e11. do

    Insert

    CENPB CRISPRi guide RNA
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. ELL1 CtoA

    Plasmid

    #127279

    Purpose

    Testing if ELL1 C to A mutation knock out the action on Myc as it does in human

    Depositor

    Christine Milcarek

    Insert

    ELL1 (NM_007924 plus CEYLHNK to AEYLHNK) (ELL Mouse)
    

    Use

    Tags

    Twin strep-tag

    Expression

    Mammalian

    Mutation

    CEYLHNK to AEYLHNK by Q5 mutagenesis

    Promoter

    EF1a

    Available since

    Nov. 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pSBbi-Pur-Lyn-AktAR2-EV-D

    Plasmid

    #125200

    Purpose

    SB-transposon plasmid for stable expression of unphosphorylatable "dead" version of lipid raft-targeted Lyn-AktAR2-EV variant of FRET-based AKT activity reporter

    Depositor

    Eric Davis

    Insert

    Lyn-AktAR2-EV-D
    

    Use

    Transposon

    Tags

    EV linker, cpVenus[E172] and Lyn tag (GCIKSKRKD),…

    Expression

    Mammalian

    Mutation

    FoxO1 Thr-24 changed to Val rendering this inacti…

    Promoter

    EF1a/RPBSA

    Available since

    May 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. HA-mRIPK3 AAAA/D143N-Flag/cHA-pTRIPZ vector

    Plasmid

    #73706

    Purpose

    lentiviral expression of mRIPK3 mutant

    Depositor

    Francis Chan

    Insert

    RIPK3 (Ripk3 Mouse)
    

    Use

    Lentiviral

    Tags

    Flag and HA

    Expression

    Mammalian

    Mutation

    448-AAAA-451 and D143N

    Promoter

    CMV/tet

    Available since

    March 28, 2016

    Availability

    Academic Institutions and Nonprofits only

35. MSCV-PM mir shHoxB9 a

    Plasmid

    #27021

    Depositor

    Joan Massague

    Article

    Nguyen et al Cell. 2009 Jul 10. 138(1):51-62.

    Insert

    mir HoxB9 2A
    

    Use

    RNAi and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 24, 2011

    Availability

    Academic Institutions and Nonprofits only

36. scramble shRNA

    Plasmid

    #1864

    Purpose

    3rd gen lentiviral negative control vector containing scrambled shRNA

    Depositor

    David Sabatini

    Article

    Sarbassov et al Science 2005 Feb 18;307(5712):1098-101.

    Insert

    scramble
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 5, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pCW57-CMV-ssRFP-GFP-KDEL

    Plasmid

    #128257

    Purpose

    Fluorescence reporter to monitor ER-phagy activity

    Depositor

    Noboru Mizushima

    Article

    Chino et al Mol Cell. 2019 Apr 11. pii: S1097-2765(1

    Insert

    ssRFPGFPKDEL
    

    Use

    Lentiviral

    Tags

    RFPGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pMRX-IP-HaloTag7-LC3

    Plasmid

    #184899

    Purpose

    Stable expression of HaloTag7-LC3 in mammalian cells to measure autophagic flux

    Depositor

    Noboru Mizushima

    Article

    Yim et al Elife. 2022 Aug 8;11. pii: 78923. doi: 1

    Insert

    LC3B
    

    Use

    Tags

    HaloTag7

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pMSCV-Blasticidin

    Plasmid

    #75085

    Purpose

    retrovirus-mediated gene transfer into mammalian cells

    Depositor

    David Mu

    Article

    Kendall et al Proc Natl Acad Sci U S A. 2007 Oct 16;10

    Type

    Empty backbone

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pTK511

    Plasmid

    #114686

    Purpose

    Express Mem-BFP-iLID

    Depositor

    Tomomi Kiyomitsu

    Article

    Okumura et al Elife. 2018 May 31;7. pii: 36559. doi: 1

    Insert

    Mem-BFP-iLID
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge