We narrowed to 10,761 results for: T7

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pEPQDKN0329

Plasmid

#162315

Purpose

TEV protease plasmid for T7-driven E. coli cell-free protein synthesis with detection via HiBiT

Depositor

Nicola Patron

Article

Dudley et al Synth Biol (Oxf). 2021 Sep 11;6(1):ysab0

Insert

T7prom-RBS-NET-TEVprotease-HiBiT

Use

Synthetic Biology

Available Since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pEPQD0KN0244

Plasmid

#162282

Purpose

Acceptor for golden gate assembly with BsaI overhangs GCTT-CCAT, adds a N-terminal expression tag. Generates plasmids for T7-driven E. coli cell-free protein synthesis

Depositor

Nicola Patron

Article

Dudley et al Synth Biol (Oxf). 2021 Sep 11;6(1):ysab0

Type

Empty backbone

Expression

Bacterial

Available Since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pEPQD0KN0024

Plasmid

#162281

Purpose

Acceptor for golden gate assembly with BsaI overhangs GCTT-AATG, adds a N-terminal expression tag. Generates plasmids for T7-driven E. coli cell-free protein synthesis

Depositor

Nicola Patron

Article

Dudley et al Synth Biol (Oxf). 2021 Sep 11;6(1):ysab0

Type

Empty backbone

Expression

Bacterial

Available Since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pBEL1840

Plasmid

#155150

Purpose

Expresses MlaF-6xHis

Depositor

Gira Bhabha, Damian Ekiert

Article

Kolich et al Elife. 2020 Jun 30;9. pii: 60030. doi: 1

Insert

MlaF-6xHis

Promoter

T7 promoter

Available Since

Dec. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pBEL1245

Plasmid

#155139

Purpose

Expresses MlaF in E. coli

Depositor

Gira Bhabha, Damian Ekiert

Article

Kolich et al Elife. 2020 Jun 30;9. pii: 60030. doi: 1

Insert

6xHis-TEV-mlaF

Promoter

T7 promoter

Available Since

Dec. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pBEL1246

Plasmid

#155141

Purpose

Expresses MlaB in E. coli

Depositor

Gira Bhabha, Damian Ekiert

Article

Kolich et al Elife. 2020 Jun 30;9. pii: 60030. doi: 1

Insert

6xHis-TEV-mlaB

Promoter

T7 promoter

Available Since

Dec. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pRSFDuet1-tglA(C51)

Plasmid

#134674

Purpose

Expresses His6-TglA-Cys in E. Coli

Depositor

Wilfred van der Donk

Article

Ting et al Science. 2019 Jul 19;365(6450):280-284.

Insert

TglA(C51)

Tags

His6

Expression

Bacterial

Mutation

insertion of cysteine 51

Promoter

T7

Available Since

July 17, 2020

Availability

Academic Institutions and Nonprofits only

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pACYCDuet1-ammA

Plasmid

#134682

Purpose

Expresses His6-AmmA in E. Coli

Depositor

Wilfred van der Donk

Article

Ting et al Science. 2019 Jul 19;365(6450):280-284.

Insert

ammA

Tags

His6

Expression

Bacterial

Promoter

T7

Available Since

July 14, 2020

Availability

Academic Institutions and Nonprofits only

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pET28a-ammB4

Plasmid

#134688

Purpose

Expresses His6-AmmB4 in E. Coli

Depositor

Wilfred van der Donk

Article

Ting et al Science. 2019 Jul 19;365(6450):280-284.

Insert

ammB4

Tags

His6

Expression

Bacterial

Promoter

T7

Available Since

July 14, 2020

Availability

Academic Institutions and Nonprofits only

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pET28a-ammB3

Plasmid

#134687

Purpose

Expresses His6-AmmB3 in E. Coli

Depositor

Wilfred van der Donk

Article

Ting et al Science. 2019 Jul 19;365(6450):280-284.

Insert

ammB3

Tags

His6

Expression

Bacterial

Promoter

T7

Available Since

July 14, 2020

Availability

Academic Institutions and Nonprofits only

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pET28a-ammB2

Plasmid

#134686

Purpose

Expresses His6-AmmB2 in E. Coli

Depositor

Wilfred van der Donk

Article

Ting et al Science. 2019 Jul 19;365(6450):280-284.

Insert

ammB2

Tags

His6

Expression

Bacterial

Promoter

T7

Available Since

July 14, 2020

Availability

Academic Institutions and Nonprofits only

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pET28a-ammB1

Plasmid

#134685

Purpose

Expresses His6-AmmB1 in E. Coli

Depositor

Wilfred van der Donk

Article

Ting et al Science. 2019 Jul 19;365(6450):280-284.

Insert

ammB1

Tags

His6

Expression

Bacterial

Promoter

T7

Available Since

July 14, 2020

Availability

Academic Institutions and Nonprofits only

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pWDY1228

Plasmid

#138594

Purpose

Expresses NatL2-R156K in E.coli

Depositor

James Naismith

Article

Song et al Angew Chem Int Ed Engl. 2020 Jan 5. doi:

Insert

phenylacetate-coenzyme A ligase

Tags

6-His

Mutation

changed Arginine 156 to Lysine

Promoter

T7

Available Since

March 16, 2020

Availability

Academic Institutions and Nonprofits only

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pWDY1211

Plasmid

#138591

Purpose

Expresses NatL2 in E.coli

Depositor

James Naismith

Article

Song et al Angew Chem Int Ed Engl. 2020 Jan 5. doi:

Insert

phenylacetate-coenzyme A ligase

Tags

6-His

Expression

Bacterial

Promoter

T7

Available Since

March 16, 2020

Availability

Academic Institutions and Nonprofits only

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pET28a-KinK

Plasmid

#139596

Purpose

expresses KinK in E. coli

Depositor

Emily Balskus

Article

Wang et al Nat Commun. 2018 Sep 11;9(1):3687. doi:

Insert

kinK

Tags

hexahistidine

Expression

Bacterial

Promoter

T7

Available Since

March 5, 2020

Availability

Academic Institutions and Nonprofits only

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pET-Himar-dCas9-mammalian

Plasmid

#138414

Purpose

Protein purification of Himar-dCas9 with 3x-FLAG, SV40 NLS, and 6x-His tag

Depositor

Harris Wang

Article

Chen et al CRISPR J. 2019 Dec;2(6):376-394. doi: 10

Insert

Himar-dCas9

Tags

3xFLAG, 6xHis, and SV40 NLS

Expression

Bacterial

Promoter

T7

Available Since

March 3, 2020

Availability

Academic Institutions and Nonprofits only

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pET28a-AlpJ

Plasmid

#139598

Purpose

expresses AlpJ in E. coli

Depositor

Emily Balskus

Article

Wang et al J Am Chem Soc. 2017 Mar 1;139(8):2864-28

Insert

alpJ

Tags

N terminal hexahistidine

Expression

Bacterial

Promoter

T7

Available Since

March 2, 2020

Availability

Academic Institutions and Nonprofits only

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pET28a-KinO1

Plasmid

#139597

Purpose

expresses KinO1 in E. coli

Depositor

Emily Balskus

Article

Wang et al J Am Chem Soc. 2017 Mar 1;139(8):2864-28

Insert

kinO1

Tags

N terminal hexahistidine

Expression

Bacterial

Promoter

T7

Available Since

March 2, 2020

Availability

Academic Institutions and Nonprofits only

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pET28a-KinL

Plasmid

#139595

Purpose

expresses KinL in E. coli

Depositor

Emily Balskus

Article

Wang et al Nat Commun. 2018 Sep 11;9(1):3687. doi:

Insert

kinL

Tags

hexahistidine

Expression

Bacterial

Promoter

T7

Available Since

March 2, 2020

Availability

Academic Institutions and Nonprofits only

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pET28a-KinM

Plasmid

#139594

Purpose

expresses KinM in E. coli

Depositor

Emily Balskus

Article

Wang et al Nat Commun. 2018 Sep 11;9(1):3687. doi:

Insert

kinM

Tags

hexahistidine

Expression

Bacterial

Promoter

T7

Available Since

March 2, 2020

Availability

Academic Institutions and Nonprofits only

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