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AAV-hSyn-DIO-mCReP

Plasmid

#167503

Purpose

Conditional expression of the eIF2alpha phophatase, CReP (Ppp1r15b)

Depositor

Nicole Calakos

Article

Helseth et al Science. 2021 Apr 23;372(6540). pii: 372

Insert

Ppp1r15b (Ppp1r15b Mouse)

Use

AAV and Cre/Lox

Tags

Myc

Expression

Mammalian

Promoter

hSyn

Available Since

June 7, 2021

Availability

Academic Institutions and Nonprofits only

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TRBV20-1

Plasmid

#123454

Purpose

construction of TCR

Depositor

Catherine Wu

Article

Hu et al Blood. 2018 Nov 1;132(18):1911-1921. doi

Insert

TRBV20-1 (TRBV20-1 Human)

Expression

Bacterial

Mutation

WT

Available Since

May 21, 2019

Availability

Academic Institutions and Nonprofits only

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AAV-actin prom-dsRed-adra2a.1227.shRNA

Plasmid

#67880

Purpose

expresses dsRed and shRNA to knock down adra2a receptors

Depositor

William Wisden

Article

Zhang et al Nat Neurosci. 2015 Apr;18(4):553-61. doi

Insert

dsRed and shRNA to knock down the mouse adra2 receptor

Use

AAV

Expression

Mammalian

Promoter

chicken beta actin

Available Since

Sept. 11, 2015

Availability

Academic Institutions and Nonprofits only
pCRISPR-HOT-mNeon-Stop-PGK-mCherry-CAAX

Plasmid

#240800

Purpose

CRISPR-HOT donor plasmid for mNeonGreen fusion protein co-expressing membrane-bound mCherry via PGK promoter

Depositor

Hans Clevers

Article

Krueger et al Science. 2025 Sep 4;389(6764):eadr8753.

Insert

mNeonGreen mCherry-CAAX

Use

CRISPR

Expression

Mammalian

Mutation

none

Promoter

PGK

Available Since

Aug. 1, 2025

Availability

Academic Institutions and Nonprofits only

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ESR1-luciferase

Plasmid

#113351

Purpose

evaluate FOXA1 DIV motif enhancer activity near 50 kb of ESR1 gene

Depositor

Ralf Jauch

Article

Wang et al Nucleic Acids Res. 2018 Jun 20;46(11):54

Insert

ESR1-enhancer (ESR1 Human)

Use

Luciferase

Available Since

Sept. 12, 2018

Availability

Academic Institutions and Nonprofits only

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Hchk2 (kin-)-PUNI10

Plasmid

#41908

Depositor

Stephen Elledge

Article

Matsuoka et al Science. 2007 May 25;316(5828):1160-6.

Insert

CHK2 (CHEK2 Human)

Expression

Bacterial

Mutation

kinase dead (D347A)

Available Since

June 11, 2014

Availability

Academic Institutions and Nonprofits only
pAAV-TRE-DIO-GCaMP6s

Plasmid

#183809

Purpose

his plasmid is for use with neuronal cell-type selective activity tagging. The GCaMP6 expression requires both neural activity and Cre recombinase.

Depositor

William Wisden

Article

Yu et al Science. 2022 Jul;377(6601):63-72. doi:

Insert

AAV transgene TetO promoter-DIO/flex-GCaMP6

Use

AAV, Cre/Lox, and Mouse Targeting

Expression

Mammalian

Available Since

July 5, 2022

Availability

Academic Institutions and Nonprofits only

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pCEP4-FLAG-CCR5

Plasmid

#98949

Purpose

Mammalian expression plasmid for N-terminal FLAG-tagged human CCR5

Depositor

Erik Procko

Article

Heredia et al J Immunol. 2018 Apr 20. pii: jimmunol.18

Insert

CCR5 (CCR5 Human)

Tags

FLAG (dykdddd) epitope tag and Signal/leader sequ…

Expression

Mammalian

Mutation

Full-length, wildtype

Promoter

CMV

Available Since

April 24, 2018

Availability

Academic Institutions and Nonprofits only

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pGEX Zap70(NC)-SH2

Plasmid

#46535

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

Zap70(NC) (ZAP70 Human)

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 1-268

Promoter

Tac

Available Since

Aug. 12, 2013

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-R-diLACCO1

Plasmid

#208102

Purpose

Control biosensor for intracellular L-lactate

Depositor

Robert Campbell, Marie-Eve Paquet

Article

Nasu et al Nat Commun. 2023 Oct 27;14(1):6598. doi:

Insert

eLACCO2.1

Use

AAV

Available Since

Nov. 14, 2023

Availability

Academic Institutions and Nonprofits only

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FSW-HRP-V5-LRRTM1

Plasmid

#82536

Purpose

Lentiviral vector for expressing HRP-LRRTM1 in neurons

Depositor

Alice Ting

Article

Loh et al Cell. 2016 Aug 25;166(5):1295-1307.e21.

Insert

LRRTM1 (LRRTM1 Human)

Use

Lentiviral

Tags

HRP and V5

Expression

Mammalian

Promoter

Human Synapsin

Available Since

Sept. 21, 2016

Availability

Academic Institutions and Nonprofits only

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HA-MBP-TEVpCS-ADGRE1 CTFdeltaTA-FLAG

Plasmid

#217722

Purpose

Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

Depositor

Stephen Blacklow

Article

Dates et al Cell Chem Biol. 2024 Aug 15;31(8):1542-1

Insert

ADGRE1 (EMR1 Human)

Tags

FLAG and HA

Expression

Mammalian

Mutation

Deletion of residues 1-590

Available Since

April 16, 2024

Availability

Academic Institutions and Nonprofits only

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HA-MBP-TEVpCS-ADGRF4 CTFdeltaTA-FLAG

Plasmid

#217730

Purpose

Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

Depositor

Stephen Blacklow

Article

Dates et al Cell Chem Biol. 2024 Aug 15;31(8):1542-1

Insert

ADGRF4 (ADGRF4 Human)

Tags

FLAG and HA

Expression

Mammalian

Mutation

Deletion of residues 1-390

Available Since

April 16, 2024

Availability

Academic Institutions and Nonprofits only

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HA-MBP-TEVpCS-ADGRF3 CTFdeltaTA-FLAG

Plasmid

#217729

Purpose

Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

Depositor

Stephen Blacklow

Article

Dates et al Cell Chem Biol. 2024 Aug 15;31(8):1542-1

Insert

ADGRF3 (ADGRF3 Human)

Tags

FLAG and HA

Expression

Mammalian

Mutation

Deletion of residues 1-758

Available Since

April 16, 2024

Availability

Academic Institutions and Nonprofits only

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pGEX SHP-1(NC)-SH2

Plasmid

#46496

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

SHP-1(NC) (PTPN6 Human)

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 1-221

Promoter

Tac

Available Since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

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TRBV14

Plasmid

#123449

Purpose

construction of TCR

Depositor

Catherine Wu

Article

Hu et al Blood. 2018 Nov 1;132(18):1911-1921. doi

Insert

TRBV14 (TRBV14 Human)

Expression

Bacterial

Mutation

WT

Available Since

May 21, 2019

Availability

Academic Institutions and Nonprofits only

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TRBV2

Plasmid

#123409

Purpose

construction of TCR

Depositor

Catherine Wu

Article

Hu et al Blood. 2018 Nov 1;132(18):1911-1921. doi

Insert

TRBV2 (TRBV2 Human)

Expression

Bacterial

Mutation

WT

Available Since

May 10, 2019

Availability

Academic Institutions and Nonprofits only

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TRAV19

Plasmid

#123391

Purpose

construction of TCR

Depositor

Catherine Wu

Article

Hu et al Blood. 2018 Nov 1;132(18):1911-1921. doi

Insert

TRAV19 (TRAV19 Human)

Expression

Bacterial

Mutation

WT

Available Since

May 6, 2019

Availability

Academic Institutions and Nonprofits only

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TRAV8-4

Plasmid

#123377

Purpose

construction of TCR

Depositor

Catherine Wu

Article

Hu et al Blood. 2018 Nov 1;132(18):1911-1921. doi

Insert

TRAV8-4 (TRAV8-4 Human)

Expression

Bacterial

Mutation

WT

Available Since

May 6, 2019

Availability

Academic Institutions and Nonprofits only

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HH02-LP Clone 2 heavy chain

Plasmid

#192175

Purpose

Clone 2 heavy chain (HH02)

Depositor

Sidi Chen

Article

Peng et al Nat Commun. 2022 Mar 28;13(1):1638. doi:

Insert

Clone 2 heavy chain (HH02)

Expression

Mammalian

Mutation

NA

Available Since

Dec. 8, 2022

Availability

Academic Institutions and Nonprofits only

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