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pmCherry-C1 mCherry-NLS

Plasmid

#58476

Purpose

Expresses a nuclear-targeted mCherry control construct, on a CMV reporter

Depositor

Dyche Mullins

Article

Belin et al Elife. 2015 Aug 19;4. doi: 10.7554/eLife

Insert

3X NLS with 3X stop codons

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 18, 2015

Availability

Academic Institutions and Nonprofits only

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pHR myc-MBP-SspB-iRFP-NLS

Plasmid

#223286

Purpose

An SspB-tagged maltose binding protein (MBP) with an infrared fluorescent protein for visualization. Used to demonstrate condensate dissolution using the OptoMBP system.

Depositor

Jared Toettcher

Article

Brumbaugh-Reed et al bioRxiv 2024.01.16.575860

Insert

Myc-MBP-SspB-irFP-NLS

Use

Lentiviral

Expression

Mammalian

Available Since

Aug. 14, 2024

Availability

Academic Institutions and Nonprofits only

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Myc tag WT PTB

Plasmid

#23024

Depositor

Douglas Black

Article

Xie et al Proc Natl Acad Sci U S A. 2003 Jul 22. 1

Insert

PTB1 (PTBP1 Human)

Tags

Myc

Expression

Mammalian

Available Since

June 4, 2010

Availability

Academic Institutions and Nonprofits only
pFastBac WT SOX

Plasmid

#192055

Purpose

Expression vector for WT SOX

Depositor

Britt Glaunsinger

Article

Hartenian et al Nucleic Acids Res. 2023 Jan 11;51(1):182

Insert

WT SOX (ORF37)

Expression

Insect

Promoter

Polyhedrin

Available Since

Dec. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pDEST-eGFP-C1-VPS35

Plasmid

#163622

Purpose

human VPS35 cDNA C-terminally tagged with eGFP

Depositor

Josef Kittler

Article

Halff et al Cell Rep. 2019 Nov 26;29(9):2599-2607.e6

Insert

human VPS35 (VPS35 Human)

Tags

GFP

Expression

Mammalian

Available Since

Jan. 15, 2021

Availability

Academic Institutions and Nonprofits only

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plenti-CAG -IRES-GFP

Plasmid

#69047

Purpose

Lentiviral backbone for GFP coexpression

Depositor

William Kaelin

Article

Lu et al Science. 2014 Jan 17;343(6168):305-9. do

Type

Empty backbone

Use

Lentiviral

Tags

IRES-GFP

Expression

Mammalian

Available Since

Oct. 17, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV4-Tax WT

Plasmid

#23284

Depositor

Warner Greene

Article

Beraud et al Mol Cell Biol. 1994 Feb . 14(2):1374-82.

Insert

Tax

Expression

Mammalian

Available Since

April 29, 2010

Availability

Academic Institutions and Nonprofits only

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pAAV-GFAP104-ChR2-mCherry

Plasmid

#58892

Purpose

AAV-mediated expression of ChR2-mCherry under the GFAP promoter

Depositor

Edward Boyden

Article

Perea et al Nat Commun. 2014;5:3262. doi: 10.1038/nc

Insert

ChR2-mCherry

Use

AAV

Tags

mCherry

Expression

Mammalian

Promoter

GFAP104

Available Since

Aug. 14, 2014

Availability

Academic Institutions and Nonprofits only

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pTP1122

Plasmid

#104159

Purpose

Bacterial expression plasmid of anti-mouse IgG1 Fc nanobody TP1107 (3x Cysteines)

Depositor

Dirk Görlich

Article

Pleiner et al J Cell Biol. 2018 Mar 5;217(3):1143-1154

Insert

Anti-mouse IgG1 Fc specific nanobody TP1107 (3xCysteines)

Tags

14xHistidine tag and NEDD8 from Brachypodium dist…

Expression

Bacterial

Available Since

Dec. 21, 2017

Availability

Academic Institutions and Nonprofits only

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pCAG-Cre-IRES2-GFP

Plasmid

#26646

Purpose

Mammalian coexpression of nls-Cre and EGFP from the CAG promoter

Depositor

Anjen Chenn

Article

Woodhead et al J Neurosci. 2006 Nov 29;26(48):12620-30.

Insert

Cre

Use

Cre/Lox

Tags

GFP and IRES2

Expression

Mammalian

Promoter

CAG

Available Since

Dec. 2, 2010

Availability

Academic Institutions and Nonprofits only

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pSUPER.retro.puro-shNC

Plasmid

#113535

Purpose

Control retroviral vector.

Depositor

Blerta Xhemalce

Article

Shelton et al Cell Rep. 2018 Feb 6;22(6):1374-1383. do

Insert

shNC (scrambled control shRNA)

Use

Retroviral

Available Since

July 26, 2018

Availability

Academic Institutions and Nonprofits only

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pFRT_TO_FlagHA_TIAL1

Plasmid

#106090

Purpose

gene expression of FlagHA-TIAL1

Depositor

Thomas Tuschl

Article

Meyer et al Mol Cell. 2018 Feb 15;69(4):622-635.e6.

Insert

TIAL1 (TIAL1 Human)

Tags

FlagHA

Expression

Mammalian

Available Since

Feb. 8, 2018

Availability

Academic Institutions and Nonprofits only

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mito-BFP

Plasmid

#49151

Purpose

visualization of mitochondria

Depositor

Gia Voeltz

Article

Friedman et al Science. 2011 Oct 21;334(6054):358-62. d

Insert

mitochondrial targeting signal (COX4 Budding Yeast)

Tags

BFP

Expression

Mammalian

Promoter

CMV

Available Since

May 29, 2014

Availability

Academic Institutions and Nonprofits only

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pLX-EF1a_NKX2-1_Long

Plasmid

#221483

Purpose

NKX2-1 long isoform lentiviral overexpression

Depositor

Matthew Meyerson

Article

Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

Insert

NKX2-1 (NKX2-1 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

EF1a

Available Since

July 1, 2024

Availability

Academic Institutions and Nonprofits only

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pLX-EF1a_NKX2-1

Plasmid

#221484

Purpose

NKX2-1 short isoform lentiviral overexpression

Depositor

Matthew Meyerson

Article

Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

Insert

NKX2-1 (NKX2-1 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

EF1a

Available Since

July 2, 2024

Availability

Academic Institutions and Nonprofits only

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pLX-UBC_NKX2-1

Plasmid

#221495

Purpose

NKX2-1 short isoform lentiviral overexpression using UBC promoter

Depositor

Matthew Meyerson

Article

Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

Insert

NKX2-1 (NKX2-1 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

UBC

Available Since

July 2, 2024

Availability

Academic Institutions and Nonprofits only

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3xAP1pGL3 (3xAP-1 in pGL3-basic)

Plasmid

#40342

Depositor

Alexander Dent

Article

Vasanwala et al J Immunol. 2002 Aug 15;169(4):1922-9.

Insert

3xAP-1

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Mutation

Contains three canonical AP-1 binding sites (TGAC…

Available Since

Sept. 28, 2012

Availability

Academic Institutions and Nonprofits only

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pEGFP-Sec16A

Plasmid

#36155

Purpose

Expresses Sec16A as a GFP fusion

Depositor

David Stephens

Article

Watson et al Traffic. 2006 Dec;7(12):1678-87. Epub 20

Insert

Sec16A (SEC16A Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

July 8, 2015

Availability

Academic Institutions and Nonprofits only

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pYPQ167

Plasmid

#69309

Purpose

Gateway entry vector with Cas9p

Depositor

Yiping Qi

Article

Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

Insert

Cas9p (Plant codon-optimized; high GC content at 5 prime region)

Use

CRISPR

Mutation

NLS fusion

Available Since

Sept. 14, 2015

Availability

Academic Institutions and Nonprofits only

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pMito-PAGFP-FRB.

Plasmid

#60005

Purpose

Expresses MitoTrap tagged with photo-activatable GFP in mammalian cells.

Depositor

Stephen Royle

Article

Cheeseman et al J Cell Sci. 2013 May 1;126(Pt 9):2102-13

Type

Empty backbone

Use

Knocksideways

Tags

FRB and PAGFP

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 15, 2014

Availability

Academic Institutions and Nonprofits only

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