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pETM33_Nsp15

Plasmid

#156481

Purpose

Bacterial expression of Sars-CoV2 Nsp15 protein with His-tag and GST-tag

Depositor

Ylva Ivarsson

Publication

Mihalic et al Nat Commun. 2023 Sep 13;14(1):5636. doi:

Insert

Nsp15 (ORF1ab Severe acute respiratory syndrome coronavirus 2, Synthetic)

Tags

His-GST

Expression

Bacterial

Mutation

Gene insert is codon optimized for Ecoli.

Promoter

T7/LacO

Available since

Aug. 4, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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mCherry-FKBP-PI4KB

Plasmid

#139310

Purpose

Recruitable PI4KB

Depositor

Gerry Hammond

Publication

Zewe et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

Insert

mCherry: FKBP1A(3-108): GGSA]4GG:PI4KB (PI4KB Human)

Expression

Mammalian

Available since

April 6, 2020

Availability

Academic Institutions and Nonprofits only

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EMTB-mScarletI

Plasmid

#137801

Purpose

Visualization of microtubules

Depositor

Dorus Gadella

Publication

Chertkova et al bioRxiv (2020) 160374; doi: https://doi.

Insert

Ensconsin microtubule binding domain

Expression

Mammalian

Promoter

CMV

Available since

March 30, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pME-Kaede

Plasmid

#132972

Purpose

full-length of photoconvertible kaede with stop codon in pmE Gateway vector

Depositor

Nathan Lawson

Publication

Shin et al Dev Cell. 2019 Nov 4;51(3):374-386.e5. d

Insert

Kaede

Use

Gateway vector

Available since

Dec. 4, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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TGN38 EGFP

Plasmid

#128148

Purpose

Expression of TGN38 amino acids 304-357

Depositor

Jennifer Lippincott-Schwartz

Insert

TGN38 (Tgoln2 Rat)

Tags

mEGFP

Expression

Mammalian

Mutation

Contains amino acids 304-357

Available since

Nov. 21, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pAG247 His-iFAST

Plasmid

#130808

Purpose

Expresses His-tagged iFAST in bacteria

Depositor

Arnaud Gautier

Publication

Tebo et al Biochemistry. 2018 Oct 2;57(39):5648-565

Insert

iFAST

Tags

His-tag

Expression

Bacterial

Promoter

T7

Available since

Sept. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pRSFDuet-v2 RsTAL At4CL

Plasmid

#126524

Purpose

expresses TAL (Rhodobacter sphaeroides) and 4CL (Arabidopsis thaliana paralog 1) for PYP chromophore biosynthesis

Depositor

Andrew Woolley

Publication

Brechun et al Biochemistry. 2019 Jun 11;58(23):2682-26

Inserts

tyrosine ammonia lyase
p-coumaroyl-CoA ligase (4CL1 Mustard Weed)

Tags

His

Expression

Bacterial

Promoter

T7

Available since

June 25, 2019

Availability

Academic Institutions and Nonprofits only

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Influenza-FPV-HA-mEGFP

Plasmid

#127810

Purpose

Expression vector Influenza A virus FPV (H7N1) hemagglutinin mEGFP fusion protein, plasma membrane localization

Depositor

Salvatore Chiantia

Publication

Dunsing et al Sci Rep. 2018 Jul 13;8(1):10634. doi: 10

Insert

Influenza A virus FPV (H7N1) hemagglutinin mEGFP fusion

Expression

Mammalian

Available since

June 21, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pMF1762

Plasmid

#125583

Purpose

Expresses mitochondrial roGFP2 (mt-roGFP2) in mammalian cells

Depositor

Marc Fransen

Publication

Ivashchenko et al Mol Biol Cell. 2011 May;22(9):1440-51. d

Insert

mt-roGFP2

Expression

Mammalian

Promoter

CMV

Available since

May 29, 2019

Availability

Academic Institutions and Nonprofits only

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pET_RGG-RGG

Plasmid

#124941

Purpose

Expresion of RGG-RGG

Depositor

Matthew Good, Daniel Hammer, Benjamin Schuster

Publication

Schuster et al Nat Commun. 2018 Jul 30;9(1):2985. doi:

Insert

RGG-RGG (using LAF-1 RGG domain) (laf-1 Nematode)

Tags

His

Expression

Bacterial

Available since

May 29, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pMK365 (Tet-OsTIR1-loxP-PURO-loxP)

Plasmid

#121185

Purpose

OsTIR1 introduction

Depositor

Masato Kanemaki

Publication

Yesbolatova et al Methods. 2019 Apr 23. pii: S1046-2023(18

Insert

Tet-OsTIR1-loxP-PURO-loxP

Use

CRISPR

Mutation

OsTIR1 codon optimized for human cells

Available since

Feb. 13, 2019

Availability

Academic Institutions and Nonprofits only

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STAGR_gRNAScaffold_hU6

Plasmid

#102840

Purpose

Can be used as PCR template for a STAgR reaction

Depositor

Stefan Stricker

Publication

Breunig et al PLoS One. 2018 Apr 27;13(4):e0196015. do

Insert

gRNAScaffold_hU6promoter

Promoter

hU6

Available since

Feb. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pET28-His-TEV-NQO1

Plasmid

#119058

Purpose

For expression in E.coli and affinity purification of N-terminally His tagged NQO1

Depositor

Yosef Shaul

Publication

Tsvetkov et al Proc Natl Acad Sci U S A. 2005 Apr 12;10

Insert

NAD(P)H dehydrogenase, quinone 1 (NQO1) (NQO1 Human)

Tags

6His, TEV site

Expression

Bacterial

Promoter

T7

Available since

Dec. 20, 2018

Availability

Academic Institutions and Nonprofits only

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GFP-ERK2

Plasmid

#118296

Purpose

Expression of Rat ERK2

Depositor

Philip Stork

Publication

Horgan et al Exp Cell Res. 2003 May 1;285(2):208-20.

Insert

ERK2 (Mapk1 Rat)

Tags

GFP

Expression

Mammalian

Available since

Nov. 7, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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ITPKA_3xmScarletI

Plasmid

#112960

Purpose

In vivo visualization of F-actin (can be used for colocalization studies)

Depositor

Dorus Gadella

Publication

Chertkova et al bioRxiv (2020) 160374; doi: https://doi.

Insert

ITPKA

Tags

3x mScarlet-I

Expression

Mammalian

Promoter

CMV

Available since

Aug. 28, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pJL1-mCherry

Plasmid

#102629

Purpose

In vitro expression of mCherry from the T7 promoter

Depositor

Michael Jewett

Publication

Stark et al Sci Adv. 2018 Aug 1;4(8):eaat5107. doi:

Insert

mCherry

Expression

Bacterial

Promoter

T7

Available since

Aug. 14, 2018

Availability

Academic Institutions and Nonprofits only

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VEGFR2-YFP

Plasmid

#108853

Purpose

Encodes for human VEGFR2 fluorescently labeled with eYFP on the C-Terminus via a 3 amino acid (GGS) flexible linker

Depositor

Kalina Hristova

Publication

Sarabipour et al Elife. 2016 Apr 7;5:e13876. doi: 10.7554

Insert

KDR (KDR Human)

Tags

Labeled with eYFP on the C-Terminus via a 3 amino…

Expression

Mammalian

Available since

May 10, 2018

Availability

Academic Institutions and Nonprofits only

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pHSS6hsILMi20

Plasmid

#102536

Purpose

Helper plasmid expressing Minos transposase mRNA (Drosophila hsp70 promoter)

Depositor

Michalis Averof, Charalambos Savakis

Publication

Klinakis et al Insect Mol Biol. 2000 Jun;9(3):269-75.

Insert

Minos transposase

Available since

April 10, 2018

Availability

Academic Institutions and Nonprofits only

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cyto-YFP-FKBPx5

Plasmid

#103777

Purpose

expression of iPOLYMER component in mammalian cells; has nuclear exclusion sequence

Depositor

Takanari Inoue

Publication

Nakamura et al Nat Mater. 2017 Nov 6. pii: nmat5006. do

Insert

cyto-YFP-FKBPx5

Expression

Mammalian

Promoter

CMV

Available since

Feb. 7, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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hEF1α-H2B-mVenus-IRES-mCherry PGK-Puromycin

Plasmid

#99278

Purpose

Dual fluorescent reporter - constitutive expression of mVenus (515/528) localized in the nucleus, mCherry (587/610) untargeted and visualized in the cytoplasm, and Puromycin resistance

Depositor

Jordan Miller

Publication

Calderon et al Biomater Sci. 2017 Jun 29. doi: 10.1039/

Inserts

H2B-mVenus-IRES-mCherry
Puro

Use

Lentiviral

Tags

H2B, IRES, PGK, mCherry, and mVenus

Promoter

EF1 alpha-human and PGK-mouse

Available since

Nov. 13, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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