We narrowed to 436 results for: 41594

1. hprtSAGFP

   Plasmid

   #41594

   Purpose

   In vivo single-strand annealing substrate composed of two truncated GFP genes, separated by a drug selection marker, and flanked by mouse hprt targeting sequences

   Depositor

   Maria Jasin

   Article

   Stark et al Mol Cell Biol. 2004 Nov;24(21):9305-16.

   Insert

   SA-GFP reporter
   

   Use

   Mouse Targeting; Dsb repair reporter substrate

   Expression

   Mammalian

   Available Since

   Dec. 3, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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These results may also be relevant.

1. TOE1

   Plasmid

   #156091

   Purpose

   For use in RBP tethering screen

   Depositor

   Eugene Yeo

   Article

   Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

   Insert

   TOE1 (TOE1 Human)
   

   Tags

   V5-MCP

   Expression

   Mammalian

   Mutation

   none

   Promoter

   EF-1a

   Available Since

   Nov. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. RBM41

   Plasmid

   #155902

   Purpose

   For use in RBP tethering screen

   Depositor

   Eugene Yeo

   Article

   Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

   Insert

   RBM41 (RBM41 Human)
   

   Tags

   V5-MCP

   Expression

   Mammalian

   Mutation

   none

   Promoter

   EF-1a

   Available Since

   Nov. 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pJCC_162 SpCas9 KES(1107-1109)GG

   Plasmid

   #179526

   Purpose

   For bacterial expression of SpCas9 KES(1107-1109)GG (phosphate lock loop mutant) with an N-terminal His-MBP tag

   Depositor

   Jennifer Doudna

   Article

   Cofsky et al Nat Struct Mol Biol. 2022 Apr;29(4):395-

   Insert

   SpCas9 KES(1107-1109)GG
   

   Tags

   10X His, TEV protease cleavage site, and maltose-…

   Expression

   Bacterial

   Mutation

   replaced KES (residues 1107-1109) with GG

   Available Since

   April 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pJCC_164 SpCas9 R1333A/R1335A

   Plasmid

   #179528

   Purpose

   For bacterial expression of SpCas9 R1333A/R1335A (PAM-binding arginines mutant) with an N-terminal His-MBP tag

   Depositor

   Jennifer Doudna

   Article

   Cofsky et al Nat Struct Mol Biol. 2022 Apr;29(4):395-

   Insert

   SpCas9 R1333A/R1335A
   

   Tags

   10X His, TEV protease cleavage site, and maltose-…

   Expression

   Bacterial

   Mutation

   changed arginines 1333 and 1335 to alanine

   Available Since

   April 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET15-His-∆NGlpG(C104A/M247C)

   Plasmid

   #155133

   Purpose

   Expresses N-terminally His-tagged E. coli GlpG C104A/M247C variant (residues 87-276) from pET15b

   Depositor

   Sinisa Urban

   Article

   Cho et al Nat Struct Mol Biol. 2019 Oct;26(10):910

   Insert

   GlpG (glpG Escherichia coli)
   

   Tags

   His

   Expression

   Bacterial

   Mutation

   C104A/M247C, insert codes for GlpG residues 87-276

   Promoter

   T7

   Available Since

   Sept. 8, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET22b-ecDHFR-D27S/Y100F

   Plasmid

   #110827

   Purpose

   Bacterial expression of E.coli DHFR D27S/Y100F mutant

   Depositor

   Stephen Benkovic

   Article

   Liu et al Proc Natl Acad Sci U S A. 2014 Dec 23;11

   Insert

   ecDHFR
   

   Expression

   Bacterial

   Mutation

   D27 mutated to S27, Y100 mutated to F100

   Promoter

   T7

   Available Since

   June 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDest17_MAX

   Plasmid

   #234044

   Purpose

   Bacterial expression of N-terminally 6His-tagged MAX; includes TEV cleavage site

   Depositor

   Tanja Mittag

   Article

   Yang et al Nat Struct Mol Biol. 2024 Oct;31(10):156

   Insert

   MAX (MAX Human)
   

   Tags

   6xHis

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   April 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pTET2-lucCP-3MS2

   Plasmid

   #198351

   Purpose

   Part of Firefly reporter system, encodes Firefly luciferase with MS2 stem loops in the 3'UTR

   Depositor

   Eugene Yeo

   Article

   Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

   Insert

   Firefly Luciferase
   

   Use

   Luciferase

   Expression

   Mammalian

   Promoter

   pTET2

   Available Since

   April 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. PRPF4

   Plasmid

   #155850

   Purpose

   For use in RBP tethering screen

   Depositor

   Eugene Yeo

   Article

   Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

   Insert

   PRPF4 (PRPF4 Human)
   

   Tags

   V5-MCP

   Expression

   Mammalian

   Mutation

   none

   Promoter

   EF-1a

   Available Since

   Oct. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pTET2-RlucCP-3MS2

    Plasmid

    #198354

    Purpose

    Part of Renilla reporter system, encodes Renilla luciferase with MS2 stem loops in the 3'UTR

    Depositor

    Eugene Yeo

    Article

    Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

    Insert

    Renilla Luciferase
    

    Use

    Luciferase

    Expression

    Mammalian

    Promoter

    pTET2

    Available Since

    April 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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