We narrowed to 443 results for: DHFR

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pCMV-miRFP670-eDHFR(69K6)

Plasmid

#178859

Purpose

Mammalian expression of miRFP670-eDHFR(69K6)

Depositor

Shinya Tsukiji

Article

Suzuki et al Cell Chem Biol. 2022 Sep 15;29(9):1446-1

Insert

miRFP670-eDHFR(69K6)

Expression

Mammalian

Promoter

CMV

Available Since

July 14, 2022

Availability

Academic Institutions and Nonprofits only

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CAG.INBox-mCherry-INBox-eDHFR

Plasmid

#176203

Purpose

Double INBox (INCENP 819-918) recruitment responsible for targeted Aurora B activation and recruitment using TMP-Halo dimerization system

Depositor

Michael Lampson

Article

Chen et al J Cell Biol. 2021 Jun 7;220(6). pii: 212

Insert

INBox-mCherry-INBox-eDHFR

Expression

Mammalian

Available Since

Feb. 11, 2022

Availability

Academic Institutions and Nonprofits only

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CAG.mCherry-INBox(AAA)-eDHFR

Plasmid

#176207

Purpose

INBox TSS(892-894)-mutant replaced by AAA responsible for targeted Aurora B recruitment without activation using TMP-Halo dimerization system

Depositor

Michael Lampson

Article

Chen et al J Cell Biol. 2021 Jun 7;220(6). pii: 212

Insert

mCherry-INBoxAAA-eDHFR

Expression

Mammalian

Available Since

Nov. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pSL2-DHFR-mCherry-loxP.dna

Plasmid

#166235

Purpose

Floxed DHFR fused with mCherry

Depositor

David Sibley

Insert

mCherry

Mutation

none

Available Since

April 8, 2021

Availability

Academic Institutions and Nonprofits only

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KN501: pMVP (L3-L2) ecDHFR DD + WPRE

Plasmid

#121793

Purpose

pMVP L3-L2 entry plasmid, contains ecDHFR DD + WPRE for 3- or 4-component MultiSite Gateway Pro assembly. Allows fusion of C-term ecDHFR degron domain (stablized by TMP) to gene in lentivirus vectors

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Insert

ecDHFR Degron Domain + WPRE

Use

Synthetic Biology; Pmvp gateway entry plasmid

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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PB-PGK-Cit-tvhc-DHFR

Plasmid

#116048

Purpose

Reporter plasmid for measuring protease activity

Depositor

Michael Elowitz

Article

Gao et al Science. 2018 Sep 21;361(6408):1252-1258

Insert

Cit-tvhc-DHFR

Expression

Mammalian

Available Since

Dec. 4, 2018

Availability

Academic Institutions and Nonprofits only

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PB-PGK-Cit-tehc-DHFR

Plasmid

#116046

Purpose

Reporter plasmid for measuring protease activity

Depositor

Michael Elowitz

Article

Gao et al Science. 2018 Sep 21;361(6408):1252-1258

Insert

Cit-tehc-DHFR

Expression

Mammalian

Available Since

Oct. 18, 2018

Availability

Academic Institutions and Nonprofits only

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PB-CMV-Cit-tehc-DHFR

Plasmid

#116058

Purpose

Reporter plasmid for measuring protease activity

Depositor

Michael Elowitz

Article

Gao et al Science. 2018 Sep 21;361(6408):1252-1258

Insert

Cit-tehc-DHFR

Expression

Mammalian

Available Since

Oct. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pET22b-ecDHFR-dCys-T46C

Plasmid

#110543

Purpose

Bacterial expression of E.coli DHFR C85A/C152S/T46C mutant

Depositor

Stephen Benkovic

Article

Liu et al J Am Chem Soc. 2014 Jul 23;136(29):10349

Insert

ecDHFR

Expression

Bacterial

Mutation

T46 mutated to C46, C85 mutated to A85, C152 muta…

Promoter

T7

Available Since

June 7, 2018

Availability

Academic Institutions and Nonprofits only

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pET22b-ecDHFR-dCys-L54C

Plasmid

#110542

Purpose

Bacterial expression of E.coli DHFR C85A/C152S/L54C mutant

Depositor

Stephen Benkovic

Article

Liu et al J Am Chem Soc. 2014 Jul 23;136(29):10349

Insert

ecDHFR

Expression

Bacterial

Mutation

L54 mutated to C54, C85 mutated to A85, C152 muta…

Promoter

T7

Available Since

June 7, 2018

Availability

Academic Institutions and Nonprofits only

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