Addgene

We narrowed to 1,459 results for: Mbp

Showing: 441 - 460 of 1459 results

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pCIneo-V5-SBP-MBP-HsNot4_64-575_AG

Plasmid

#148766

Purpose

Mammalian Expression of HsNot4_64-575

Depositor

Elisa Izaurralde

Article

Keskeny et al Genes Dev. 2019 Feb 1;33(3-4):236-252. d

Insert

HsNot4_64-575 (CNOT4 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pMJ273: pRS426-His-MBP-GtFz1_PSP1

Plasmid

#205258

Purpose

S. cerevisiae expression vector for GtFz1 reprogrammed guide (targeting PSP1)

Depositor

Feng Zhang

Article

Saito et al Nature. 2023 Jun 28. doi: 10.1038/s41586

Insert

GtFz1

Use

Tags

10xHis, MBP

Expression

Yeast

Mutation

Promoter

Available since

Aug. 15, 2023

Availability

Academic Institutions and Nonprofits only

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pMJ127: pRS426-His-MBP-NlovFz2_PSP1

Plasmid

#205257

Purpose

S. cerevisiae expression vector for NlovFz2 reprogrammed guide (targeting PSP1)

Depositor

Feng Zhang

Article

Saito et al Nature. 2023 Jun 28. doi: 10.1038/s41586

Insert

NlovFz2

Use

Tags

10xHis, MBP

Expression

Yeast

Mutation

Promoter

Available since

Aug. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pET-3a-His-MBP-TEV-VC_Cas1_WT

Plasmid

#120364

Purpose

Expresses wildtype V-C Cas1

Depositor

Jennifer Doudna

Article

Wright et al Mol Cell. 2019 Feb 21;73(4):727-737.e3.

Insert

VC_Cas1

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 12, 2021

Availability

Academic Institutions and Nonprofits only

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pET-3a-His-MBP-TEV-VD_Cas1_WT

Plasmid

#120365

Purpose

Expresses wildtype V-D Cas1

Depositor

Jennifer Doudna

Article

Wright et al Mol Cell. 2019 Feb 21;73(4):727-737.e3.

Insert

VD_Cas1

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 12, 2021

Availability

Academic Institutions and Nonprofits only

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MBP-TEV-VMA Model vector

Plasmid

#86590

Purpose

This can be used to make MBP-TEV-Cterm with SapI and PstI; and can also make N-term-VMA with NdeI and SapI

Depositor

Sharon Rozovsky

Article

Liu et al J Am Chem Soc. 2017 Feb 10. doi: 10.1021

Insert

VMA intein

Use

Tags

His6-MBP

Expression

Bacterial

Mutation

Original SapI site from pMal was mutated out

Promoter

Tac

Available since

Feb. 24, 2017

Availability

Industry, Academic Institutions, and Nonprofits

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pAAV-EF1a-CCre-MBP1-WPRE

Plasmid

#193917

Purpose

Expresses one of the components of Cre-DOR_N6C1 (RFP-dependent Cre)

Depositor

Tatsushi Onaka

Article

Inutsuka et al Commun Biol. 2022 Sep 16;5(1):979. doi:

Insert

CCre-MBP1

Use

AAV, Affinity Reagent/ Antibody, Cre/Lox, and Syn…

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Jan. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-NCre-MBP6-WPRE

Plasmid

#193916

Purpose

Expresses one of the components of Cre-DOR_N6C1 (RFP-dependent Cre)

Depositor

Tatsushi Onaka

Article

Inutsuka et al Commun Biol. 2022 Sep 16;5(1):979. doi:

Insert

NCre-MBP6

Use

AAV, Affinity Reagent/ Antibody, Cre/Lox, and Syn…

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Jan. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pSL0376 (His-MBP-StrepII-TniQ)

Plasmid

#130641

Purpose

Expresses V. cholerae CAST TniQ from a T7 promoter, with N-terminal His10-MBP-TEVsite-StrepII for purification.

Depositor

Samuel H. Sternberg

Article

Klompe et al Nature. 2019 Jul;571(7764):219-225. doi:

Insert

VchTniQ

Use

CRISPR; Transposon

Tags

His10-MBP-TEVsite-StrepII on TniQ

Expression

Bacterial

Mutation

Promoter

T7

Available since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pMG035 MBP-Axin∆BCD-His

Plasmid

#154053

Purpose

Expresses MBP-Axin∆BCD-His (human Axin with aa465-518 deletion as a fusion protein with MBP and His- tags) in E.coli

Depositor

Jesse Zalatan

Article

Gavagan et al Cell Syst. 2020 Jun 24;10(6):515-525.e5.

Insert

Axin1 (AXIN1 Human)

Use

Tags

His6 and MBP

Expression

Bacterial

Mutation

Promoter

Tac

Available since

Jan. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pZL1-ZF - myelin basic protein a (mbpa)

Plasmid

#103007

Purpose

Plasmid template for making in-situ hybridization probe for myelin basic protein a (mbpa) gene zf-fj330a07.y1.

Depositor

Marnie Halpern

Article

Brosamle et al Glia. 2002 Jul;39(1):47-57.

Insert

myelin basic protein (mbpa Zebrafish)

Use

Tags

Expression

Mutation

Promoter

Sp6

Available since

Dec. 18, 2017

Availability

Academic Institutions and Nonprofits only
His8:MBP-tev-Gly-eK-Flv

Plasmid

#83383

Purpose

bacterial expression vector pVP16 containing a derivative of E. coli lacZ fused with E. coli flavin binding protein MioC to be used in N-end rule in vitro ubiquitination studies

Depositor

Nico Dissmeyer

Insert

flavin binding protein MioC

Use

Synthetic Biology

Tags

3xHA, 8xHis, and MBP

Expression

Bacterial

Mutation

Promoter

Available since

April 21, 2017

Availability

Academic Institutions and Nonprofits only

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pACE-MBP-FBXL17 (310–701)

Plasmid

#228369

Purpose

express human FBXL17 in insect cells, such as Trichoplusia ni Hi5

Depositor

Ning Zheng

Article

Cao et al Cell. 2024 Oct 30:S0092-8674(24)01188-7.

Insert

FBXL17 (FBXL17 Human)

Use

Tags

MBP

Expression

Insect

Mutation

Promoter

Available since

May 15, 2025

Availability

Academic Institutions and Nonprofits only

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pLIC-His6-MBP-TEV-Rgs14

Plasmid

#236395

Purpose

Expresses His6-MBP-TEV-Rgs14 in E. coli

Depositor

John Hepler

Article

Brown et al J Biol Chem. 2015 Apr 3;290(14):9037-49.

Insert

Rattus norvegicus regulator of G-protein signaling 14 (Rgs14 Rat)

Use

Tags

hexahistidine (H6) tag and maltose-binding protei…

Expression

Bacterial

Mutation

Promoter

Available since

May 1, 2025

Availability

Academic Institutions and Nonprofits only

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pYY166 pcDNA3.4-His-MBP-hAID

Plasmid

#231942

Purpose

Expression of His-MBP-AID fusion protein in mammalian cells

Depositor

Fei-Long Meng

Article

Wang et al Cell. 2023 May 11;186(10):2193-2207.e19.

Insert

AID (AICDA Human)

Use

Tags

His-MBP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 21, 2025

Availability

Academic Institutions and Nonprofits only

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pACYCDuet-AtTIR1-Myc-MBP-AtIAA6-HA

Plasmid

#222708

Purpose

Expression AtTIR1-Myc and MBP-AtIAA6-HA in in bacterial cells

Depositor

Lei Wu

Article

Pu et al Biomolecules. 2024 Sep 25;14(10):1209. d

Inserts

AtTIR1 (TIR1 Mustard Weed)
AtIAA6 (IAA6 Mustard Weed)

Use

Tags

HA, MBP, and myc

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 31, 2025

Availability

Academic Institutions and Nonprofits only

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pEmal10H-MBP-EWSR1-449-513

Plasmid

#199439

Purpose

Expresses RG2 (the second RG-rich region, 449-513) of EWS

Depositor

David Libich

Article

Selig et al Biopolymers. 2023 May;114(5):e23536. doi

Insert

EWSR1 (EWSR1 Human)

Use

Tags

His10 and MBP

Expression

Bacterial

Mutation

deleted amino acids 1-448, 514-656

Promoter

T7

Available since

Oct. 28, 2024

Availability

Academic Institutions and Nonprofits only

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HA-MBP-TEVpCS-ADGRC1 CTFdeltaTA-FLAG

Plasmid

#217717

Purpose

Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

Depositor

Stephen Blacklow

Article

Dates et al bioRxiv 2022.07.14.500097

Insert

CELSR1 (CELSR1 Human)

Use

Tags

FLAG and HA

Expression

Mammalian

Mutation

Deletion of residues 1-2453

Promoter

Available since

Aug. 19, 2024

Availability

Academic Institutions and Nonprofits only

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HA-MBP-TEVpCS-ADGRG7 CTF-FLAG

Plasmid

#217705

Purpose

Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

Depositor

Stephen Blacklow

Article

Dates et al bioRxiv 2022.07.14.500097

Insert

ADGR7 (ADGRG7 Human)

Use

Tags

FLAG and HA

Expression

Mammalian

Mutation

Deletion of residues 1-415

Promoter

Available since

Aug. 19, 2024

Availability

Academic Institutions and Nonprofits only

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HA-MBP-TEVpCS-ADGRB2 CTFdeltaTA-FLAG

Plasmid

#217715

Purpose

Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

Depositor

Stephen Blacklow

Article

Dates et al bioRxiv 2022.07.14.500097

Insert

ADGRB2 (BAI2 Human)

Use

Tags

FLAG and HA

Expression

Mammalian

Mutation

Deletion of residues 1-917

Promoter

Available since

Aug. 19, 2024

Availability

Academic Institutions and Nonprofits only

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