We narrowed to 6,763 results for: poly
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Plasmid#65812Purposeexpression of eIF4G-MS2 fusion.DepositorInserteIF4G (EIF4G1 Human)
TagsMS2 stem loops and V5ExpressionMammalianMutationdelN83 (functionally behaves as the WT protein)PromoterCMVAvailable SinceSept. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCI-MS2V5-eRF3a F76a
Plasmid#65811Purposeexpression of eRF3a-MS2 fusion. F76A mutation abolishes binding to PABPC1DepositorInserteRF3a (GSPT1 Human)
TagsMS2 stem loops and V5ExpressionMammalianMutationF76aPromoterCMVAvailable SinceSept. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAG32-3HA-3FLAG
Plasmid#154081PurposeFor C-terminal 3xHA-3xFLAG-protein tagging in S. pombe using hphMX4 as resistance marker.DepositorInsert3xHA-3xFLAG-hygR
UsePcr-based tagging in yeastTags3xHA-3xFLAGAvailable SinceSept. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLV-EF1A-PARP14-1535-1601-Hygro
Plasmid#238100PurposeExpression of structurally predicted WWE domain of PARP14 with EGFP on C terminalDepositorInsertPARP14 (PARP14 Human)
UseLentiviralTagsEGFPExpressionMammalianMutationaa 1535-1601 onlyPromoterEF1alphaAvailable SinceAug. 8, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLV-Hygro-EF1A-TRIP12(749-836)-EGFP
Plasmid#196229PurposeEGFP fused to the C-terminus of a WWE domain within TRIP12 & a hygromycin resistance cassetteDepositorInsertC-terminal EGFP tagged TRIP12(749- 836)
UseLentiviralTagsEGFP and Myc tagExpressionMammalianPromoterEF1AAvailable SinceAug. 8, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLV-Hygro-EF1A-DTX2(8-97)-EGFP
Plasmid#196230PurposeEGFP fused to the C-terminus of first WWE domain within Deltex2 & a hygromycin resistance cassetteDepositorInsertC-terminal EGFP tagged DTX2(8-97)
UseLentiviralTagsEGFP and Myc tagExpressionMammalianPromoterEF1AAvailable SinceAug. 8, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLV-Hygro-EF1A-DTX2(8-174)-EGFP
Plasmid#196232PurposeEGFP fused to the C-terminus of tandem WWE domains within Deltex2 & a hygromycin resistance cassetteDepositorInsertDTX2(8-174)-EGFP
UseLentiviralTagsEGFP and Myc tagExpressionMammalianPromoterEF1AAvailable SinceAug. 8, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLV-Hygro-EF1A-Af1521(K35E/Y145R)-EGFP
Plasmid#196235PurposeEGFP fused to the C-terminus of Af1521(K35E/Y145R) macrodomain & a hygromycin resistance cassetteDepositorInsertC-terminal EGFP tagged Af1521(K35E/Y145R)
UseLentiviralTagsEGFPExpressionMammalianMutationK35E and Y145RPromoterEF1AAvailable SinceAug. 8, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLV-Hygro-EF1A-RNF146(100-182)-myc
Plasmid#196236PurposeRNF146(100-182) WWE domain with a myc tag fused to the C terminus & a hygromycin resistance cassetteDepositorInsertRNF146(100-182) encoding a C-terminal myc tag
UseLentiviralTagsmyc tagExpressionMammalianPromoterEF1AAvailable SinceAug. 8, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLV-Hygro-EF1A-RNF146(92-168)-EGFP
Plasmid#196228PurposeEGFP fused to the C-terminus of a WWE domain within RNF146 & a hygromycin resistance cassetteDepositorInsertRNF146(92-168)-EGFP
UseLentiviralTagsEGFP and Myc tagExpressionMammalianPromoterEF1AAvailable SinceAug. 8, 2025AvailabilityAcademic Institutions and Nonprofits only -
PABPC1_544-626_pGEX6P1
Plasmid#209790PurposeBacterial expression of PABPC1 Mlle domainDepositorInsertMlle domain of PABPC1 (PABPC1 Human)
TagsGSTExpressionBacterialMutationaa 544-626 onlyPromotertacAvailable SinceNov. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMVQA
Plasmid#197894PurposepCR®2.1-TOPO® backbone with 3' end of HIV-1(HXB2:9045 till poly-A (20))DepositorInsertHIV-1 3' end (HXB2: 9045 till poly-A(10)
ExpressionBacterialAvailable SinceJune 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pENTR4 Ta-SRO1 JN177
Plasmid#192546PurposepENTR plasmid Triticum aestivum SRO1, full CDS, cultivar JN177, no stop codonDepositorInsertSIMILAR TO RCD ONE 1 (SRO1), cultivar Jinan 177 (JN177)
UseGateway entry vectorAvailable SinceDec. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pENTR4 Ta-sro1 SR3
Plasmid#192547PurposepENTR plasmid Triticum aestivum sro1, full CDS, cultivar SR3, no stop codonDepositorInsertSIMILAR TO RCD ONE 1 (SRO1), cultivar Shanrong No. 3 (SR3)
UseGateway entry vectorAvailable SinceDec. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
ABEmax-Puro V3
Plasmid#226956PurposeABEmax plasmid enabling A:T to G:C base editing using a single plasmid. sgRNA uses 3TC scaffold for increased sgRNA expression.DepositorTypeEmpty backboneUseCRISPRExpressionMammalianAvailable SinceMarch 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
HaloTag-LaminA
Plasmid#231894PurposeExpression of HaloTag-tagged LaminA to visualize the nuclear laminaDepositorAvailable SinceFeb. 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
Pdg459-eSp(1.1) V3
Plasmid#226965PurposeCBh-eSpCas9(1.1)-2A-Puro, and 2X hU6-sgRNA (Sp) with a BbsI golden gate cloning backbone for sgRNA. sgRNA uses 3TC scaffold for increased sgRNA expression.DepositorTypeEmpty backboneUseCRISPRExpressionMammalianAvailable SinceOct. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEX-4T-3-GST-Tev-Af1521-myc
Plasmid#196239PurposeN-terminal GST fusion of Af1521(WT) with a TEV protease site located between the GST tag and Af1521 and a myc tag on the C terminusDepositorInsertN-terminal GST fusion of Af1521(WT) with a TEV protease site between GST and Af1521 encoding a C-terminal myc tag
TagsGST tag and myc tagExpressionBacterialAvailable SinceAug. 8, 2025AvailabilityAcademic Institutions and Nonprofits only -
pACEBac1-PARP1-Flag-His-WT
Plasmid#111572PurposeExpresses full length PARP1 in Sf9 (insect) cellsDepositorInsertPARP1 (PARP1 Human)
TagsC-terminal 6xHis and C-terminal FlagExpressionInsectPromoterpolyhedrin promoterAvailable SinceJuly 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pOPINE GFP nanobody:Halo:His6
Plasmid#111090PurposeBacterial expression of a fusion protein consisting of a GFP nanobody (Dr. Brett Collins, Addgene plasmid # 49172), the Halo tag and a His6 tag.DepositorInsertGFP Nanobody
TagsHalo and His6ExpressionBacterial, Insect, and Mamm…PromoterT7 promoterAvailable SinceJune 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
PDG459 V3
Plasmid#226958PurposeCBh-SpCas9-2A-Puro, and 2X hU6-sgRNA (Sp) with BbsI golden gate cloning backbone dual gRNAs. sgRNA uses 3TC scaffold for increased sgRNA expression.DepositorTypeEmpty backboneUseCRISPRExpressionMammalianAvailable SinceOct. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
SaCas9 GFP V3
Plasmid#226962PurposeCBh-SaCas9-2A-GFP, and hU6-sgRNA (Sa) with a BbsI golden gate cloning backbone for sgRNA. sgRNA uses 3TC scaffold for increased sgRNA expression.DepositorTypeEmpty backboneUseCRISPRExpressionMammalianAvailable SinceOct. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
SaCas9v3-Puro
Plasmid#178813PurposeSaCas9 with 2A-Puro, and a cloning backbone for sgRNA. sgRNA scaffold seqeuence has been modified for increased sgRNA expression (v3).DepositorTypeEmpty backboneUseCRISPRExpressionMammalianPromoterCbh (Cas9-2A Puro) and U6 (gRNA)Available SinceAug. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
PDG458 V3
Plasmid#226959PurposeCBh-SpCas9-2A-GFP, and 2X hU6-sgRNA (Sp) with BbsI golden gate cloning backbone dual gRNAs. sgRNA uses 3TC scaffold for increased sgRNA expression.DepositorTypeEmpty backboneUseCRISPRExpressionMammalianAvailable SinceOct. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
PX458 V3
Plasmid#226957PurposeCBh-SpCas9-2A-GFP, and hU6-sgRNA (Sp) with a BbsI golden gate cloning backbone for sgRNA. sgRNA uses 3TC scaffold for increased sgRNA expression.DepositorTypeEmpty backboneUseCRISPRExpressionMammalianAvailable SinceOct. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
7Gli:GFP
Plasmid#110494Purposereporter of Hedgehog signaling activityDepositorInsert7 repeats of the Gli binding site GAACACCCA
UseLentiviralExpressionMammalianPromoter7 repeats of Gli biding siteAvailable SinceMay 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLV-EF1A-DTX2-1-178-EGFP-Hygro
Plasmid#238094PurposeExpression of structurally predicted WWE domain of DTX2 with EGFP on C terminalDepositorInsertDTX2 (DTX2 Human)
UseLentiviralTagsEGFPExpressionMammalianMutationaa 1-178 onlyPromoterEF1alphaAvailable SinceAug. 8, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCMV-PARP1-3xFlag-7xKQ
Plasmid#111576PurposeExpresses full length PARP1 with K to Q mutations in automodification domain in mammalian cellsDepositorInsertPARP1 (PARP1 Human)
Tags3xFlagExpressionMammalianMutationlysine to glutamine mutation at the following ami…PromoterCMVAvailable SinceJuly 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
PX458 eSp(1.1) V3
Plasmid#226963PurposeCBh-eSpCas9(1.1)-2A-GFP, and hU6-sgRNA (Sp) with a BbsI golden gate cloning backbone for sgRNA. sgRNA uses 3TC scaffold for increased sgRNA expression.DepositorTypeEmpty backboneUseCRISPRExpressionMammalianAvailable SinceOct. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEX4T2 RANK-L
Plasmid#108571PurposeEncodes C-terminal part of RANKL (aa158-aa316) with a GST N-terminal tagDepositorInsertRANKL (receptor activator of nuclear factor kappa-B ligand) (Tnfsf11 Mouse)
TagsGSTExpressionMammalianMutationaa158-aa316PromoterPtac (trp/lac)Available SinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only