We narrowed to 2,467 results for: PUC19
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Plasmid#133791PurposeU6 promoter sgRNA entry vector used for all St1Cas9 sgRNAs (clone spacer oligos into BsmBI cassette) - similar to V1 sgRNA architecture from Carter et al. biorxiv 2018DepositorInsertSt1Cas9 sgRNA entry vector
ExpressionMammalianMutationV1 sgRNA architecture from Carter et al. biorxiv …PromoterU6Available SinceMarch 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pUC19 gene 60 thru gap + Cys
Plasmid#49161PurposeTemplate for generating gene 60 mRNA truncated two codons after coding gap + CysDepositorInsertBacteriophage T4 gene 60
Mutationgene 60 mRNA truncated two codons after coding ga…PromoterT7Available SinceFeb. 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
pUC19-MAFA-PQR-NLS-tdTomato-P2A-puro-PGK-puro
Plasmid#121174PurposeDonor template for CRISPR-Cas9 mediated generation of MAFA-NLS-tdTomato reporter cell linesDepositorInsertMAFA-PQR-NLS-tdTomato-P2A-Puro-PGK-Puro
UseCRISPRExpressionMammalianAvailable SinceFeb. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pUC19_CoV-2_frD2 ΔE6 3* Δ7a/H1 WSN ΔTM
Plasmid#253161PurposeFor recombinant virus rescue; encodes fragment D2 of SARS-CoV-2 (nt25422-29867), with deletion of envelope, orf6, orf7a;stop codon at AA213 in orf3a; Influenza H1 (WSN) ΔTM replaces orf7aDepositorInsertCoV-2 frD2 with Influenza H1 WSN
ExpressionBacterialAvailable SinceMay 6, 2026AvailabilityAcademic Institutions and Nonprofits only -
pUC19_CoV-2_ frD2 ΔE6 3* Δr7a/H1 PR8 ΔTM
Plasmid#253165PurposeFor recombinant virus rescue; encodes fragment D2 of SARS-CoV-2 (nt25422-29867), with deletion of envelope, orf6, orf7a;stop codon at AA213 in orf3a; Influenza H1 (PR8) ΔTM replaces orf7aDepositorInsertCoV-2 frD2 with Influenza H1 PR8
ExpressionBacterialAvailable SinceMay 6, 2026AvailabilityAcademic Institutions and Nonprofits only -
pUC19-HYG-BES1-VSG-228-telo
Plasmid#245104PurposeInserts VSG-228 in T. brucei cells at Lister427 BES1DepositorInsertVSG-228
UseOtherAvailable SinceApril 8, 2026AvailabilityAcademic Institutions and Nonprofits only -
pUC19-HDRT-CD3z-truncCAR(anti-CD19)
Plasmid#215758PurposeHDR template to insert a truncated CD3-zeta-deficient CD19-CAR into CD247 exon 2DepositorAvailable SinceJune 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTR-348 pUC19-tNGFR-P2A-ICOS N
Plasmid#186066PurposeKnockin of truncated NGFR to target geneDepositorAvailable SinceJuly 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTR-337 pUC19-tNGFR-P2A-CTLA4 N
Plasmid#186054PurposeKnockin of truncated NGFR to target geneDepositorAvailable SinceJuly 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTR-342 pUC19-tNGFR-P2A-CD28 N
Plasmid#186056PurposeKnockin of truncated NGFR to target geneDepositorAvailable SinceJuly 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pUC19 KGA HA5'-mKO2-P2A-Zeo-3'
Plasmid#110408PurposeHomology donor for glutaminase KGA isoform knock-in, mKO2-P2A-ZeoDepositorAvailable SinceFeb. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUC19_mRuby-3xFLAG-NLS-3xSV40-poly(A)_loxP-PGK-Puro-loxP
Plasmid#195505PurposeT-REX17 donor plasmid to generate locus specific integration of mRuby followed by 3xSV40 poly(A) into Exon 1 of human lncRNA T-REX17DepositorInsertpT-REX-mRuby-3xFLAG-NLS-3xSV40-poly(A)_loxP-PGK-Puro-loxP_T-REX(Ex1)
UseCRISPR; Donor vectorTagsmRuby-3xFLAG-NLS-3xSV40-poly(A)ExpressionMammalianAvailable SinceFeb. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pUC19-GCG-T2A-NLS-EmGFP-P2A-puro-PGK-puro
Plasmid#121175PurposeDonor template for CRISPR-Cas9 mediated generation of GCG-NLS-EmGFP reporter cell linesDepositorInsertGCG-T2A-NLS-EmGFP-P2A-Puro-PGK-Puro
UseCRISPRExpressionMammalianAvailable SinceJuly 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pIG-724pUC19-tNGFR-P2A-EVER2
Plasmid#186105PurposeKnockin of truncated NGFR to target geneDepositorAvailable SinceJuly 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pIG-748pUC19-tNGFR-P2A-AP3B1
Plasmid#186106PurposeKnockin of truncated NGFR to target geneDepositorAvailable SinceJuly 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pIG-734pUC19-tNGFR-P2A-PIK3R1
Plasmid#186107PurposeKnockin of truncated NGFR to target geneDepositorAvailable SinceJuly 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pIG-710pUC19-tNGFR-P2A-IL10R1
Plasmid#186108PurposeKnockin of truncated NGFR to target geneDepositorAvailable SinceJuly 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pIG-713pUC19-tNGFR-P2A-CD45
Plasmid#186109PurposeKnockin of truncated NGFR to target geneDepositorAvailable SinceJuly 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pIG-717pUC19-tNGFR-P2A-CD3E
Plasmid#186111PurposeKnockin of truncated NGFR to target geneDepositorAvailable SinceJuly 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pIG-737pUC19-tNGFR-P2A-CD18
Plasmid#186113PurposeKnockin of truncated NGFR to target geneDepositorAvailable SinceJuly 28, 2022AvailabilityAcademic Institutions and Nonprofits only