We narrowed to 2,335 results for: PUC19

1. pUC-ODN2

   Plasmid

   #208643

   Purpose

   Empty vector for long single strand DNA (up to 1.5k) preparation. Further remove BsaI and BsmBI site in pUC-ODN (Addgene #208642).

   Depositor

   Quanfu Ma

   Type

   Empty backbone

   Use

   Long single strand dna production

   Available Since

   Jan. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pPRDM15-donor

   Plasmid

   #176373

   Purpose

   CRISPR donor plasmid to create GFP fusion proteins

   Depositor

   Barbara Stranger, Kevin White

   Insert

   PRDM15 (PRDM15 Human)
   

   Use

   CRISPR

   Tags

   GFP

   Available Since

   Nov. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pZIC3-donor

   Plasmid

   #176361

   Purpose

   CRISPR donor plasmid to create GFP fusion proteins

   Depositor

   Barbara Stranger, Kevin White

   Insert

   ZIC3 (ZIC3 Human)
   

   Use

   CRISPR

   Tags

   GFP

   Available Since

   Nov. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pTHAP12-donor

   Plasmid

   #176348

   Purpose

   CRISPR donor plasmid to create GFP fusion proteins

   Depositor

   Barbara Stranger, Kevin White

   Insert

   THAP12 (THAP12 Human)
   

   Use

   CRISPR

   Tags

   GFP

   Available Since

   Nov. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pPOU6F1-donor

   Plasmid

   #176359

   Purpose

   CRISPR donor plasmid to create GFP fusion proteins

   Depositor

   Barbara Stranger, Kevin White

   Insert

   POU6F1 (POU6F1 Human)
   

   Use

   CRISPR

   Tags

   GFP

   Available Since

   Nov. 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pZNF608-donor

   Plasmid

   #176362

   Purpose

   CRISPR donor plasmid to create GFP fusion proteins

   Depositor

   Barbara Stranger, Kevin White

   Insert

   ZNF608 (ZNF608 Human)
   

   Use

   CRISPR

   Tags

   GFP

   Available Since

   Nov. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAW8-aid-spo5DSR-Turg1:kanMX6

   Plasmid

   #169358

   Purpose

   C-terminal aid-spo5DSR degron cassette with kanMX6 marker for S. pombe

   Depositor

   Tony Carr

   Insert

   auxin-inducible degron (aid)
   

   Expression

   Yeast

   Available Since

   June 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pZNF624-donor

   Plasmid

   #132511

   Purpose

   CRISPR donor plasmid to create GFP fusion proteins

   Depositor

   Kevin White

   Insert

   ZNF624 (ZNF624 Human)
   

   Use

   CRISPR

   Tags

   GFP

   Mutation

   none

   Available Since

   March 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pZNF8-donor

   Plasmid

   #136689

   Purpose

   CRISPR donor plasmid to create GFP fusion proteins

   Depositor

   Kevin White

   Insert

   ZNF8 (ZNF8 Human)
   

   Use

   CRISPR

   Tags

   GFP

   Expression

   Mammalian

   Available Since

   March 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. LncRNA overexpression EF1α_BbsI_SV40 polyA_EGFP

    Plasmid

    #219828

    Purpose

    Overexpression vector for non-coding RNAs driven by EF1α promoter with GFP. Cloning using BbsI.

    Depositor

    Philipp Maass

    Type

    Empty backbone

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    July 31, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. AbVec2.0-mIghg3

    Plasmid

    #127160

    Purpose

    Expression of secretory immunoglobulin heavy chains in mammalian cells, mouse (C57BL/6), IgG3 isotype

    Depositor

    Hedda Wardemann

    Insert

    Ighg3 (Ighg3 Mouse)
    

    Expression

    Mammalian

    Promoter

    hCMV IE1 gene promoter

    Available Since

    Nov. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLinker-6xHA-HXGPRT-LoxP

    Plasmid

    #86552

    Purpose

    The plasmid is used for endogenous tagging using CRISPR technology in Toxoplasma

    Depositor

    David Sibley

    Insert

    Linker-6xHA-HGPRT-LoxP
    

    Use

    Expression in toxoplasma gondii

    Available Since

    Aug. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSB-Bleo Gal4 tBFP

    Plasmid

    #139190

    Purpose

    Gal4-inducible tag blue fluorescent protein (tBFP) in pSB-Bleo Gal4 Sleeping Beauty backbone.

    Depositor

    Neal Devaraj

    Insert

    tBFP
    

    Use

    Transposon

    Expression

    Mammalian

    Promoter

    Gal4 CMV and STAT3

    Available Since

    July 17, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. hROSA26_Hygro_hPGK1_mScarlet-I_hCdt1(1-100)

    Plasmid

    #191389

    Purpose

    Donor for targeted integration of hCdt1(1-100) probe to the hROSA26 safe harbor locus

    Depositor

    Yannick Doyon

    Insert

    hCdt1(1-100)
    

    Tags

    mScarlet-I

    Expression

    Mammalian

    Available Since

    Feb. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pUC119

    Plasmid

    #50010

    Purpose

    pUC cloning vector

    Depositor

    Joachim Messing

    Article

    Vieira et al Methods Enzymol. 1987;153:3-11.

    Type

    Empty backbone

    Use

    Cloning vector

    Available Since

    July 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. hROSA26_Hygro_hPGK1_mScarlet-I_hCdt1(30-120)

    Plasmid

    #191391

    Purpose

    Donor for targeted integration of hCdt1(30-120) probe to the hROSA26 safe harbor locus

    Depositor

    Yannick Doyon

    Insert

    hCdt1(30-120)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    mScarlet-I

    Expression

    Mammalian

    Available Since

    Feb. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pPATZ1-donor

    Plasmid

    #132522

    Purpose

    CRISPR donor plasmid to create GFP fusion proteins

    Depositor

    Kevin White

    Insert

    PATZ1 (PATZ1 Human)
    

    Use

    CRISPR

    Tags

    GFP

    Mutation

    none

    Available Since

    March 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. hSLBP_mNG_FS_Donor

    Plasmid

    #191387

    Purpose

    Donor for C-terminal tagging of endogenous hSLBP

    Depositor

    Yannick Doyon

    Insert

    mNeonGreen(mNG) + FS(3xFLAG_TwinStrep)Tag for hSLBP tagging at the C-terminus (SLBP Human, Synthetic)
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Feb. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pUA97

    Plasmid

    #69340

    Purpose

    It encodes a fluorescent protein that can be fused to a gene of interest using SEC CRISPR strategy

    Depositor

    Oliver Hobert

    Insert

    ceBFP SEC 3xFlag
    

    Tags

    3xFlag and ceBFP (mTagBFP)

    Expression

    Worm

    Mutation

    sqt-1(e1350)

    Available Since

    Dec. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pINER5

    Plasmid

    #185895

    Purpose

    In vivo gene amplification (HamAmp) of nerolidol synthetic module at RPL25 locus (15 copy).

    Depositor

    Claudia Vickers

    Insert

    PRPL25(Arm1)>Kl.LEU2>TKl.LEU2-PGAL1>ERG20>TRPL3-TRPL25(Arm3)-ARS305-PSk.GAL2>YFAST-EVBR1.2A-AcNES1>TRPL41B-PPDA1>RPL25(part;Arm2
    

    Expression

    Yeast

    Mutation

    None

    Available Since

    Aug. 25, 2022

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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