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Plasmid#103252PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-148b-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-148b-3p target (MIR148B Human)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGEX Shark-tSH2
Plasmid#126672PurposeExpression of Shark tandem SH2 domains SNAP tagged for use as FRET reporterDepositorInsertShark tandem SH2 domains
UseTagsExpressionBacterialMutationPromoterAvailable sinceJuly 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHR-LAT-Y6F YYYY-mCitrine (XSB586)
Plasmid#78515PurposeLentiviral vector for expressing LAT-Y6F YYYY-mCitrine (3 Grb2 binding sites)DepositorInsertLAT (LAT Human)
UseLentiviralTagsmCitrineExpressionMammalianMutationPromoterAvailable sinceAug. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-484
Plasmid#103552PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-484 target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-484 target (MIR484 Human)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
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pMT Draper-ITAM only-GFP
Plasmid#126656PurposeInducible expression of Draper-ITAM only-GFP mutant in S2 cellsDepositorInsertDraper with Cytoplasmic ITAM residues Tyr all others mutated to Phe
UseTagsExpressionInsectMutationCytoplasmic ITAM residues Tyr all others mutated …PromoterAvailable sinceJune 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-195-5p
Plasmid#103312PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-195-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-195-5p target (MIR195 Human)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
ATF4 19: (SL)RLuc.5’ATF4.GFP
Plasmid#21865DepositorInsertATF4 (Atf4 Mouse)
UseTagsEGFPExpressionMammalianMutationStem Loop upstream of ATF4PromoterAvailable sinceOct. 1, 2009AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-15b-5p
Plasmid#103269PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-15b-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-15b-5p target (MIR15B Human)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
roGFP_iE_C204S_pQE30
Plasmid#48632PurposeBacterial expression vector encoding for the 6XHis tagged insensitive variant of fluorescent redox probe roGFPiE with Cysteine 204 to Serine mutation (roGFP C147S, E147b, C204)DepositorInsertroGFP1
UseTagsHisTagExpressionBacterialMutationinsertion E147B, c204SPromoterAvailable sinceOct. 8, 2013AvailabilityAcademic Institutions and Nonprofits only -
AIRAP 4: AIRAP.GST.V3.pCDNA3
Plasmid#21794DepositorInsertAIRAP (Zfand2a Mouse)
UseTagsGSTExpressionMammalianMutationPromoterAvailable sinceSept. 10, 2009AvailabilityAcademic Institutions and Nonprofits only -
pRS426-TUB1-internal6xHis - human TUBA1a Cterminal tail
Plasmid#60392PurposeExpression of chimeric yeast alpha tubulin (TUB1) - internal 6xHis with human alpha tubulin Cterminal tail (TUBA1a) using a GAL promoterDepositorInsertTUB1 (TUB1 Budding Yeast, Synthetic)
UseTagsExpressionYeastMutationinternal 6xHis (see supplement figure 4), amino a…PromoterGALAvailable sinceJan. 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-17-5p
Plasmid#103274PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-17-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-17-5p target (MIR17 Human)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-130a-3p
Plasmid#103216PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-130a-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-130a-3p target (MIR130A Human)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-28-3p
Plasmid#103385PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-28-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-28-3p target (MIR28 Human)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-510-3p
Plasmid#103592PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-510-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-510-3p target (MIR510 Human)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-339-3p
Plasmid#103452PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-339-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-339-3p target (MIR338 Human)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-630
Plasmid#103690PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-630 target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-630 target (MIR630 Human)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-633
Plasmid#103691PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-633 target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-633 target (MIR633 Human)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-376c-5p
Plasmid#103500PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-376c-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-376c-5p target (MIR376C Human)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only