We narrowed to 5,514 results for: donor

1. AAV EGFP donor pDY0404

   Plasmid

   #219862

   Purpose

   AAV EGFP donor

   Depositor

   Jonathan Gootenberg, Omar Abudayyeh

   Article

   Fell et al Nat Protoc. 2024 Dec 15. doi: 10.1038/s4

   Insert

   EGFP
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDonor-FMR1-CGG-BsmBI

   Plasmid

   #222966

   Purpose

   0CGG with BsmBI site for inserting CGG repeats

   Depositor

   Xinyu Zhao

   Article

   Sirois et al Cell Rep. 2024 Jun 11;43(6):114330. doi:

   Insert

   FMR1 homologous template (no CGG repeats) with cloning site (FMR1 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   CGG repeats deleted from 5' UTR, BsmBI site …

   Promoter

   Available since

   Sept. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGS96_EGFP(A206K)-I3-01 AAVS donor

   Plasmid

   #211865

   Purpose

   I3-01 transgene integration at AAVS1 safe harbor locus

   Depositor

   Iain Cheeseman

   Insert

   I3-01
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   N/A

   Promoter

   Available since

   May 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDonor-Prf1-ires-mCherry

   Plasmid

   #209073

   Purpose

   Targeting vector for the mouse Prf1 locus to correct prf1 gene

   Depositor

   Klaus Rajewsky

   Article

   Li et al Sci Immunol. 2024 Feb 2;9(92):eadi0042.

   Insert

   Prf1-ires-mCherry HDR template (parts of Prf1 with mutated PAM) (Prf1 Mouse)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDonor-PRF1-T2A-cDNA

   Plasmid

   #209076

   Purpose

   Targeting vector for the human PRF1 locus to replace exon 3 with repaired cDNA

   Depositor

   Klaus Rajewsky

   Article

   Li et al Sci Immunol. 2024 Feb 2;9(92):eadi0042.

   Insert

   PRF1-T2A-cDNA HDRT (PRF1 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. MTOR-F2108L_DARIC(1)_CD19-scFv_AAV6_Donor

   Plasmid

   #211905

   Purpose

   Vector for AAV6 donor production. Targeted CD19-scFv DARIC transgene integration to the MTOR locus with the dominant rapamycin resistance MTOR-F2108L mutation via homology-directed repair.

   Depositor

   Yannick Doyon

   Article

   Levesque et al bioRxiv 2023.09.14.557485

   Insert

   DARIC-CD19-scFv
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hPGK1

   Available since

   Feb. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pJMC-L2-Reverse-Donor-BsaI

   Plasmid

   #197843

   Purpose

   Donor vector to restore L2 destination via BsaI (reverse orientation)

   Depositor

   Daniel Voytas

   Article

   Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

   Insert

   None
   

   Use

   Unspecified

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pJMC-L2-Reverse-Donor-Esp3I

   Plasmid

   #197844

   Purpose

   Donor vector to restore L2 destination via Esp3I (reverse orientation)

   Depositor

   Daniel Voytas

   Article

   Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

   Insert

   None
   

   Use

   Unspecified

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pminiT-MED6-mEmerald donor

   Plasmid

   #197865

   Purpose

   Donor plasmid used for building MED6-mEmerald knock-in mESCs

   Depositor

   James Zhe Liu

   Article

   Dong et al bioRxiv 2022.10.26.513917

   Insert

   Med6 (Med6 Mouse)
   

   Use

   Mouse Targeting

   Tags

   mEmerald

   Expression

   Mutation

   Promoter

   NA

   Available since

   March 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pminiT-mEmerald-MED1 donor

    Plasmid

    #197867

    Purpose

    Donor plasmid used for building mEmerald-MED1 knock-in mESCs

    Depositor

    James Zhe Liu

    Article

    Dong et al bioRxiv 2022.10.26.513917

    Insert

    CRSP210 (Med1 Mouse)
    

    Use

    Mouse Targeting

    Tags

    mEmerald

    Expression

    Mutation

    Promoter

    NA

    Available since

    March 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pBlueScript II SK-SNAP donor for Hist1h3g

    Plasmid

    #186932

    Purpose

    Genomic targeting of SNAP tag at Hist1h3g C-terminal

    Depositor

    Zhiguo Zhang

    Article

    Xu et al Nat Commun. 2022 May 6;13(1):2514. doi:

    Insert

    SNAP donor for Hist1h3g (H3c8 Mouse)
    

    Use

    Tags

    SNAP

    Expression

    Mammalian

    Mutation

    Promoter

    T7

    Available since

    Aug. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMiniT:myc-GluA2 TKIT donor

    Plasmid

    #169423

    Purpose

    Donor DNA for TKIT knockin of Myc to GluA2 in mouse

    Depositor

    Richard Huganir

    Article

    Fang et al Elife. 2021 Mar 10;10. pii: 65202. doi:

    Insert

    Myc (Myc Mouse)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMC_cherry donor_Rosa target (pE002)

    Plasmid

    #149348

    Purpose

    Replace donor vector for cherry insertion using the mouse Rosa26 guide RNA

    Depositor

    Ralf Kuehn

    Article

    Danner et al Open Biol. 2021 Jan;11(1):200283. doi: 1

    Insert

    cherry
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMC_cherry donor_Polb target (pE038)

    Plasmid

    #149349

    Purpose

    Replace donor vector for cherry insertion using the human Polb guide RNA

    Depositor

    Ralf Kuehn

    Article

    Danner et al Open Biol. 2021 Jan;11(1):200283. doi: 1

    Insert

    cherry
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. HoxC-downstream-5xTetO Donor

    Plasmid

    #131341

    Purpose

    Donor template to insert 5xTet operator sequence into downstream of HoxC cluster.

    Depositor

    Danny Reinberg

    Article

    Oksuz et al Mol Cell. 2018 Jun 21;70(6):1149-1162.e5

    Insert

    HoxC-downstream-5xTetO Donor
    

    Use

    CRISPR and Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. mGDF8 donor CFP-hDF

    Plasmid

    #72590

    Purpose

    Plasmid contains left and right homology arms for mouse GDF8 flanking the mCherry-puromycin-2A-ECFP-dysferlin cassette.

    Depositor

    Renzhi Han

    Article

    Xu et al Mol Ther Nucleic Acids. 2013 Jul 30;2:e1

    Insert

    dysferlin (DYSF Human)
    

    Use

    Tags

    ECFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. hGDF8 donor CFP-hDF

    Plasmid

    #72591

    Purpose

    Plasmid contains left and right homology arms for human GDF8 flanking the mCherry-puromycin-2A-ECFP-dysferlin cassette.

    Depositor

    Renzhi Han

    Article

    Xu et al Mol Ther Nucleic Acids. 2013 Jul 30;2:e1

    Insert

    dysferlin (DYSF Human)
    

    Use

    Tags

    ECFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pHiFi Cas9-2×sgRNA (empty, donor)

    Plasmid

    #162277

    Purpose

    All-in-one vector for CRISPR/Cas9-mediated homology-independent knock-in system. The plasmid contains BPNLS-HiFi Cas9-BPNLS and two sgRNA expression cassettes.

    Depositor

    Kazuhisa Nakayama

    Article

    Fujisawa et al Biol Open. 2021 Aug 27. pii: 271969. doi

    Insert

    HiFi Cas9
    

    Use

    CRISPR

    Tags

    BPNLS and FLAG tag

    Expression

    Mammalian

    Mutation

    SpCas9 (R691A)

    Promoter

    Available since

    Aug. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. AAVS1-idCas9-KRAB-Hygro donor

    Plasmid

    #199621

    Purpose

    Donor vector for genomic targeting of a Tetracycline-inducible dCas9-KRAB cassette to the human AAVS1/PPP1R12C locus

    Depositor

    Danwei Huangfu

    Article

    Luo et al Nat Genet. 2023 Aug;55(8):1336-1346. doi

    Insert

    AAVS1-idCas9-KRAB-Hygro
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. Bxb1-GT_AIO-TetOn_Down-Tandem Donor

    Plasmid

    #229783

    Purpose

    Bxb1-GT donor plasmid with downstream tandem syntax for all-in-one doxycycline-inducible expression (empty)

    Depositor

    Richard Davis

    Article

    Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

    Insert

    LacZ; rtTA-T2A-mTagBFP2
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE and CAG

    Available since

    Feb. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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