Addgene

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hBCAM-M2

Plasmid

#203318

Purpose

Overexpression of BCAM-MCAM chimeras

Depositor

William Hahn

Article

Schrofelbauer et al Proc Natl Acad Sci U S A. 2023 Jan 3;120

Insert

hBCAM/hMUC18

Use

Tags

Flag (DYKDDDK)

Expression

Mammalian

Mutation

IgG V1-V2 of hMuc18 fused to Ig like C1-C3 of hB…

Promoter

Available since

July 28, 2023

Availability

Academic Institutions and Nonprofits only

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hBCAM-M1

Plasmid

#203317

Purpose

Overexpression of BCAM-MCAM chimeras

Depositor

William Hahn

Article

Schrofelbauer et al Proc Natl Acad Sci U S A. 2023 Jan 3;120

Insert

hBCAM/hMUC18

Use

Tags

Flag (DYKDDDK)

Expression

Mammalian

Mutation

IgG V1 of hMuc18 fused to Ig like V2-C3 of hBCAM

Promoter

Available since

July 28, 2023

Availability

Academic Institutions and Nonprofits only

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hBCAM-B4

Plasmid

#203316

Purpose

Overexpression of BCAM-MCAM chimeras

Depositor

William Hahn

Article

Schrofelbauer et al Proc Natl Acad Sci U S A. 2023 Jan 3;120

Insert

hMUC18/hBCAM

Use

Tags

Flag (DYKDDDK)

Expression

Mammalian

Mutation

IgG V1-C2 of hBCAM fused to Ig like C3 of hMUC18

Promoter

Available since

July 28, 2023

Availability

Academic Institutions and Nonprofits only

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hBCAM-B3

Plasmid

#203315

Purpose

Overexpression of BCAM-MCAM chimeras

Depositor

William Hahn

Article

Schrofelbauer et al Proc Natl Acad Sci U S A. 2023 Jan 3;120

Insert

hMUC18/hBCAM

Use

Tags

Flag (DYKDDDK)

Expression

Mammalian

Mutation

IgG V1-C1 of hBCAM fused to Ig like C2-C3 of hMUC…

Promoter

Available since

July 28, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
hBCAM-B1

Plasmid

#203313

Purpose

Overexpression of BCAM-MCAM chimeras

Depositor

William Hahn

Article

Schrofelbauer et al Proc Natl Acad Sci U S A. 2023 Jan 3;120

Insert

hMUC18/hBCAM

Use

Tags

Flag (DYKDDDK)

Expression

Mammalian

Mutation

IgG V1 of hBCAM fused to Ig like V2-C3 of hMUC18

Promoter

Available since

July 28, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
hBCAM-M3

Plasmid

#203319

Purpose

Overexpression of BCAM-MCAM chimeras

Depositor

William Hahn

Article

Schrofelbauer et al Proc Natl Acad Sci U S A. 2023 Jan 3;120

Insert

hBCAM/hMUC18

Use

Tags

Flag (DYKDDDK)

Expression

Mammalian

Mutation

IgG V1-C1 of hMuc18 fused to Ig like C2-C3 of hBC…

Promoter

Available since

July 28, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
hBCAM-B2

Plasmid

#203314

Purpose

Overexpression of BCAM-MCAM chimeras

Depositor

William Hahn

Article

Schrofelbauer et al Proc Natl Acad Sci U S A. 2023 Jan 3;120

Insert

hMUC18/hBCAM

Use

Tags

Flag (DYKDDDK)

Expression

Mammalian

Mutation

IgG V1-V2 of hBCAM fused to Ig like C1-C3 of hMU…

Promoter

Available since

July 28, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
Sha3Cas9

Plasmid

#192136

Purpose

Expresses Sha3Cas9, and cloning backbone for sgRNA

Depositor

Yongming Wang

Article

Wang et al Adv Sci (Weinh). 2022 Feb;9(4):e2104789.

Insert

Sha3Cas9

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 24, 2023

Availability

Academic Institutions and Nonprofits only

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pACT2-μ4

Plasmid

#198176

Purpose

Expression of GAL4 transcriptional activation domain (AD)-AP-4 μ4 fusion protein in yeast (yeast two-hybrid assays)

Depositor

Juan Bonifacino

Article

Guo et al Dev Cell. 2013 Nov 11;27(3):353-66. doi:

Insert

AP-4 μ4

Use

Tags

GAL4 transcriptional activation domain (AD) fragm…

Expression

Yeast

Mutation

Promoter

ADH1

Available since

April 18, 2023

Availability

Academic Institutions and Nonprofits only

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pGADT7-αC

Plasmid

#197650

Purpose

Expression of GAL4 transcriptional activation domain (AD)-AP-2 αC fusion protein in yeast (yeast two-hybrid or yeast three-hybrid assays)

Depositor

Juan Bonifacino

Article

Mattera et al J Biol Chem. 2011 Jan 21;286(3):2022-30.

Insert

AP-2 αC

Use

Tags

GAL4 transcriptional activation domain (AD) fragm…

Expression

Yeast

Mutation

Ser677T substitution

Promoter

ADH1

Available since

March 28, 2023

Availability

Academic Institutions and Nonprofits only

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pACT2-μ2

Plasmid

#198173

Purpose

Expression of GAL4 transcriptional activation domain (AD)-AP-2 μ2 fusion protein in yeast (yeast two-hybrid assays)

Depositor

Juan Bonifacino

Article

Guo et al Dev Cell. 2013 Nov 11;27(3):353-66. doi:

Insert

AP-2 μ2

Use

Tags

GAL4 transcriptional activation domain (AD) fragm…

Expression

Yeast

Mutation

Promoter

ADH1

Available since

March 15, 2023

Availability

Academic Institutions and Nonprofits only

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pSUMO chicken-cPLA2-C2-domain

Plasmid

#170753

Purpose

The plasmids contain inserts (ORFs) for expressing various members of the GlycoLipid Transfer Protein (GLTP) superfamily.

Depositor

Rhoderick Brown

Article

Hirano et al Elife. 2019 May 3;8. pii: 44760. doi: 10

Insert

PLA2G4A-C2 domain (PLA2G4A Chicken)

Use

Tags

SUMO

Expression

Bacterial

Mutation

Promoter

Available since

July 8, 2021

Availability

Academic Institutions and Nonprofits only

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pHH0103_PDCD6IP_BRO1

Plasmid

#109994

Purpose

Protein expression and purification of PDCD6IP_BRO1

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

PDCD6IP_BRO1 (PDCD6IP Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pHH0103_LRP5_Ldl-recept-b_2

Plasmid

#109807

Purpose

Protein expression and purification of LRP5_Ldl-recept-b_2

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

LRP5_Ldl-recept-b_2

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pHH0103_UBR5_PABP

Plasmid

#109877

Purpose

Protein expression and purification of UBR5_PABP

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

UBR5_PABP (UBR5 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pHH0103_NR4A2_Hormone-recep

Plasmid

#109953

Purpose

Protein expression and purification of NR4A2_Hormone-recep

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

NR4A2_Hormone-recep

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pHH0103_SPF45

Plasmid

#110044

Purpose

Protein expression and purification of SPF45

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

SPF45 (RBM17 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pHH0103_PPARA_Hormone-recep

Plasmid

#109954

Purpose

Protein expression and purification of PPARA_Hormone-recep

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

PPARA_Hormone-recep

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pHH0103_RORA_Hormone-recep

Plasmid

#109957

Purpose

Protein expression and purification of RORA_Hormone-recep

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

RORA_Hormone-recep

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pHH0103_RORC_Hormone-recep

Plasmid

#109959

Purpose

Protein expression and purification of RORC_Hormone-recep

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

RORC_Hormone-recep

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge