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EGFP-MYO10-FERM-ITGBD

Plasmid

#194855

Purpose

Express EGFP-MYO10 with mutations (S2001_F2002insA/T2009D) that interfere with ITGB binding in mammalian cells.

Depositor

Guillaume Jacquemet

Article

Miihkinen et al Cell Rep. 2021 Sep 14;36(11):109716. doi

Insert

MYO10 S2001_F2002insA/T2009D (MYO10 Human)

Tags

EGFP

Expression

Mammalian

Mutation

MYO10 has the following mutations S2001_F2002insA…

Promoter

CMV

Available Since

March 30, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1α-Flex/3'USS-ChRWR/Venus(ATG mut)

Plasmid

#197891

Purpose

Can be used to generate AAV virus that will express ChRWR/Venus in the presence of Cre and the leakage expression is significantly reduced without Cre

Depositor

Kazuto Kobayashi

Article

Matsushita et al Cell Rep Methods. 2023 Jan 18;3(2):10039

Insert

ChRWR/Venus

Use

AAV

Mutation

N/A

Promoter

EF1a

Available Since

March 15, 2023

Availability

Academic Institutions and Nonprofits only

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pMeCP2Y pc644

Plasmid

#167565

Purpose

The complete rat MeCp2 ORF is fused in frame of the NH2-terminus of the enhanced YFP (pEYFP-N1 vector; CLONTECH Laboratories, Inc.)

Depositor

Cristina Cardoso

Article

Brero et al J Cell Biol. 2005 Jun 6;169(5):733-43. d

Insert

Methyl-CpG binding protein 2 (Mecp2 Rat)

Tags

YFP

Expression

Mammalian

Promoter

CMV

Available Since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

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Coexpressed VLP NES

Plasmid

#182433

Purpose

Yeast integrative plasmid for expressing Murine polyomavirus deltaVP1 (GAL1 promoter), VP2C-ERG20 (GAL10 promoter), and VP2C-AcNES1 (GAL7 promoter). Contains uracil auxotrophic marker (K. lactis URA3)

Depositor

Claudia Vickers

Article

Cheah et al bioRxiv 2022.11.24.517869

Inserts

VP2C-FPPS
deltaVP1
VP2C-NES

Use

Synthetic Biology; Metabolic engineering

Expression

Yeast

Promoter

GAL1, GAL10, and GAL7

Available Since

Dec. 6, 2022

Availability

Academic Institutions and Nonprofits only

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Coexpressed VLP NES (M)

Plasmid

#182435

Purpose

YIp for expressing Murine polyomavirus deltaVP1 (GAL1 promoter), VP2C-ERG20(F96W-N127W) (GAL10 promoter), and VP2C-AcNES1 (GAL7 promoter). Contains uracil marker (K. lactis URA3).

Depositor

Claudia Vickers

Article

Cheah et al bioRxiv 2022.11.24.517869

Inserts

VP2C-FPPS(M)
deltaVP1
VP2C-NES

Use

Synthetic Biology; Metabolic engineering

Expression

Yeast

Promoter

GAL1, GAL10, and GAL7

Available Since

Dec. 6, 2022

Availability

Academic Institutions and Nonprofits only

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Coexpressed VLP NES (G)

Plasmid

#182477

Purpose

YIp for expressing Murine polyomavirus deltaVP1 (GAL1 promoter), VP2C-ERG20(F96C) (GAL10 promoter), and VP2C-AcNES1 (GAL7 promoter). Contains uracil marker (K. lactis URA3).

Depositor

Claudia Vickers

Article

Cheah et al bioRxiv 2022.11.24.517869

Inserts

VP2C-FPPS(G)
deltaVP1
VP2C-NES

Use

Synthetic Biology; Metabolic engineering

Expression

Yeast

Promoter

GAL1, GAL10, and GAL7

Available Since

Dec. 6, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-VTKD4-GCaMP6f

Plasmid

#178727

Purpose

AAV vector for Cre- and Flp-dependent transgene expression (CRE-ON / FLP-ON) of GCaMP6f in cortical interneurons under the control of the Dlx enhancer (Dimidschstein et al 2016)

Depositor

Jordane Dimidschstein

Article

Pouchelon et al Cell Rep Methods. 2022 May 26;2(6):10022

Insert

GCaMP6f

Use

AAV

Available Since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-VTKD6-GCaMP6f

Plasmid

#178729

Purpose

AAV vector for Cre- and Flp-dependent transgene expression (CRE-OFF / FLP-ON) of GCaMP6f in cortical interneurons under the control of the Dlx enhancer (Dimidschstein et al 2016)

Depositor

Jordane Dimidschstein

Article

Pouchelon et al Cell Rep Methods. 2022 May 26;2(6):10022

Insert

GCaMP6f

Use

AAV

Available Since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-VTKS5-GCaMP6f

Plasmid

#178731

Purpose

AAV vector for Cre- and Flp-dependent transgene expression (CRE-ON / FLP-OFF) of GCaMP6f in neurons under the control of the hSyn1 promoter (Kugler et al 2003)

Depositor

Jordane Dimidschstein

Article

Pouchelon et al Cell Rep Methods. 2022 May 26;2(6):10022

Insert

GCaMP6f

Use

AAV

Available Since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-VTKS6-GCaMP6f

Plasmid

#178732

Purpose

AAV vector for Cre- and Flp-dependent transgene expression (CRE-OFF / FLP-ON) of GCaMP6f in neurons under the control of the hSyn1 promoter (Kugler et al 2003)

Depositor

Jordane Dimidschstein

Article

Pouchelon et al Cell Rep Methods. 2022 May 26;2(6):10022

Insert

GCaMP6f

Use

AAV

Available Since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-VTKD5-dTom

Plasmid

#178716

Purpose

AAV vector for Cre- and Flp-dependent transgene expression (CRE-ON / FLP-OFF) of dTom in cortical interneurons under the control of the Dlx enhancer (Dimidschstein et al 2016)

Depositor

Jordane Dimidschstein

Article

Pouchelon et al Cell Rep Methods. 2022 May 26;2(6):10022

Insert

dTom

Use

AAV

Available Since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-VTKS2-V5-APEX2-NES

Plasmid

#178702

Purpose

AAV vector for Cre-dependent transgene expression of V5-APEX2-NES in neurons under the control of the hSyn1 promoter (Kugler et al 2003)

Depositor

Jordane Dimidschstein

Article

Pouchelon et al Cell Rep Methods. 2022 May 26;2(6):10022

Insert

V5-APEX2-NES

Use

AAV

Available Since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-VTKS3-V5-APEX2-NES

Plasmid

#178703

Purpose

AAV vector for Flp-dependent transgene expression of V5-APEX2-NES in neurons under the control of the hSyn1 promoter (Kugler et al 2003)

Depositor

Jordane Dimidschstein

Article

Pouchelon et al Cell Rep Methods. 2022 May 26;2(6):10022

Insert

V5-APEX2-NES

Use

AAV

Available Since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-VTKS4-V5-APEX2-NES

Plasmid

#178704

Purpose

AAV vector for Cre- and Flp-dependent transgene expression (CRE-ON / FLP-ON) of V5-APEX2-NES in neurons under the control of the hSyn1 promoter (Kugler et al 2003)

Depositor

Jordane Dimidschstein

Article

Pouchelon et al Cell Rep Methods. 2022 May 26;2(6):10022

Insert

V5-APEX2-NES

Use

AAV

Available Since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-VTKD4-GFP

Plasmid

#178709

Purpose

AAV vector for Cre- and Flp-dependent transgene expression (CRE-ON / FLP-ON) of GFP in cortical interneurons under the control of the Dlx enhancer (Dimidschstein et al 2016)

Depositor

Jordane Dimidschstein

Article

Pouchelon et al Cell Rep Methods. 2022 May 26;2(6):10022

Insert

GFP

Use

AAV

Available Since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-VTKD5-GFP

Plasmid

#178710

Purpose

AAV vector for Cre- and Flp-dependent transgene expression (CRE-ON / FLP-OFF) of GFP in cortical interneurons under the control of the Dlx enhancer (Dimidschstein et al 2016)

Depositor

Jordane Dimidschstein

Article

Pouchelon et al Cell Rep Methods. 2022 May 26;2(6):10022

Insert

GFP

Use

AAV

Available Since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-VTKD6-GFP

Plasmid

#178711

Purpose

AAV vector for Cre- and Flp-dependent transgene expression (CRE-OFF / FLP-ON) of GFP in cortical interneurons under the control of the Dlx enhancer (Dimidschstein et al 2016)

Depositor

Jordane Dimidschstein

Article

Pouchelon et al Cell Rep Methods. 2022 May 26;2(6):10022

Insert

GFP

Use

AAV

Available Since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-VTKD2-V5-APEX2-NES

Plasmid

#178696

Purpose

AAV vector for Cre-dependent transgene expression of V5-APEX-NES in cortical interneurons under the control of the Dlx enhancer (Dimidschstein et al 2016)

Depositor

Jordane Dimidschstein

Article

Pouchelon et al Cell Rep Methods. 2022 May 26;2(6):10022

Insert

V5-APEX2-NES

Use

AAV

Available Since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-VTKD3-V5-APEX2-NES

Plasmid

#178697

Purpose

AAV vector for Flp-dependent transgene expression of V5-APEX2-NES in cortical interneurons under the control of the Dlx enhancer (Dimidschstein et al 2016)

Depositor

Jordane Dimidschstein

Article

Pouchelon et al Cell Rep Methods. 2022 May 26;2(6):10022

Insert

V5-APEX2-NES

Use

AAV

Available Since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

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JG693: CAG-human dLbCpf1(D832A)-NLS-3xHA-3xFLAG-DmrA(X3)

Plasmid

#104570

Purpose

Mammalian expression vector for catalytically inactive Cpf1 from Lachnospiraceae bacterium (dLbCpf1) fused to three DmrA domains

Depositor

Keith Joung

Article

Tak et al Nat Methods. 2017 Oct 30. doi: 10.1038/n

Insert

dLbCpf1(D832A)-DmrA(x3)

Tags

3x FLAG, 3x HA, and NLS

Expression

Mammalian

Mutation

D832A

Promoter

CAG

Available Since

March 30, 2018

Availability

Academic Institutions and Nonprofits only

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