We narrowed to 11,766 results for: nsf
-
Plasmid#213525PurposeExpresses NSP14 of SARS-CoV-2 with a single point mutation P335HDepositorInsertSARS-CoV-2 NSP14 (ORF1ab SARS-CoV-2)
TagsHis6-sumoExpressionBacterialMutationchanged P335 to HAvailable SinceDec. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRSF_his6_sumo_SARS_CoV2_NSP14_H424R
Plasmid#213526PurposeExpresses NSP14 of SARS-CoV-2 with a single point mutation H424RDepositorInsertSARS-CoV-2 NSP14 (ORF1ab SARS-CoV-2)
TagsHis6-sumoExpressionBacterialMutationchanged H424 to RAvailable SinceDec. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
AAV-hSyn-scFVDnmt3AL_bGHpA
Plasmid#177348PurposeAAV expression of a fusion protein with scFV, catalytic domain of Dnmt3a and C terminal domain of Dnmt3l from Synapisin promoter for targeted DNA methylation editingDepositorInsertcatalytic domain of DNA Methyltransferase 3 Alpha (Dnmt3a Mouse)
UseAAVTagsC-terminal domain of mouse Dnmt3l (194–415 aa), P…ExpressionMammalianMutation598–908 aa of mouse Dnmt3aPromoterhuman SynapsineAvailable SinceSept. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
AAV-TRE-scFVDnmt3AL_bGHpA
Plasmid#177354PurposeAAV expression of a fusion protein with scFV, catalytic domain of Dnmt3a and C terminal domain of Dnmt3l from doxycycine-inducible promoter for targeted DNA methylation editingDepositorInsertcatalytic domain of DNA Methyltransferase 3 Alpha (Dnmt3a Mouse)
UseAAVTagsC-terminal domain of mouse Dnmt3l (194–415 aa), P…ExpressionMammalianMutation598–908 aa of mouse Dnmt3aPromoterTetracycline-dependent promoterAvailable SinceSept. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
AAV-TRE-scFVDnmt3A_bGHpA
Plasmid#177355PurposeAAV expression of scFV-fused catalytic domain of Dnmt3a from doxycycine-inducible promoter for targeted DNA methylation editingDepositorInsertcatalytic domain of DNA Methyltransferase 3 Alpha (Dnmt3a Mouse)
UseAAVTagsmyc and scFVExpressionMammalianMutation598–908 aa of mouse Dnmt3aPromoterTetracycline-dependent promoterAvailable SinceSept. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLX304-EZH1-Y727F
Plasmid#203582PurposeExpresses V5-tagged mutant version of EZH1 partially resistant to JQ-EZ-05 in mammalian cells.DepositorInsertEZH1 (EZH1 Human)
UseLentiviralTagsV5-taggedExpressionMammalianMutationchanged Tyrosine 727 to Phenylalanine for partial…PromoterCMVAvailable SinceFeb. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLX304-EZH1-dPSET
Plasmid#203586PurposeExpresses V5-tagged mutant version of EZH1 with deletion of aa 744-747 and partially resistant to JQ-EZ-05 in mammalian cells.DepositorInsertEZH1 (EZH1 Human)
UseLentiviralTagsV5-taggedExpressionMammalianMutationdeletion of aa 744-747 and partial resistance to …PromoterCMVAvailable SinceFeb. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLX304-EZH1-D745N
Plasmid#203585PurposeExpresses V5-tagged mutant version of EZH1 partially resistant to JQ-EZ-05 in mammalian cells.DepositorInsertEZH1 (EZH1 Human)
UseLentiviralTagsV5-taggedExpressionMammalianMutationchanged Aspartic Acid 745 to Asparagine for parti…PromoterCMVAvailable SinceFeb. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pmirGLO-3'UTR mouse Il13 mutDRACH
Plasmid#207130PurposeLuciferase vector containing 3'UTR for mouse Il13 with mutated DRACH sitesDepositorInsertInterleukin 13 (Il13) 3'UTR (Il13 Mouse)
UseLuciferaseExpressionMammalianMutationTGAGGAGAGACCATCCCTGGGCATCTCAGCTGTGGACTCATTTTCCTTT…Available SinceDec. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pT22Ubm5En2SRE4PCh(#302)
Plasmid#184089Purposecyclofen-inducible chicken EN2 (mut) activation in zebrafish permanent transgenicDepositorInsert5myc-chkEn2(SR)-ERT2-P2A-mCherry
UseZebrafish transgenesis (blue eyes)Tags5xMycMutationchkEn2: W247S, F248R; ERT2: G400V, M543A, L544A, …PromoterZf-UbiAvailable SinceJune 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pUG36-NES-EGFP-cPHx3
Plasmid#183668PurposePtdIns(3,4)P2 biosensor for expression in yeastDepositorInsertPLEKHA1 (PLEKHA1 Frog, Human)
TagsX. laevis map2k1.L(32-44)-EGFPExpressionYeastMutationAmino acids 169-329 (tandem trimer)PromoterMET25Available SinceMay 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pUG34-NES-EGFP-cPHx3
Plasmid#183669PurposePtdIns(3,4)P2 biosensor for expression in yeastDepositorInsertPLEKHA1 (PLEKHA1 Frog, Human)
TagsX. laevis map2k1.L(32-44)-EGFPExpressionYeastMutationAmino acids 169-329 (tandem trimer)PromoterMET25Available SinceMay 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
NES-mCherry-PH-BTKx1
Plasmid#183653PurposePtdIns(3,4,5)P3 biosensorDepositorInsertBTK (BTK Frog, Human)
TagsX. laevis map2k1.L(32-44)-mCherryExpressionMammalianMutationAmino acids 2-170PromoterCMVAvailable SinceMay 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGMC00001
Plasmid#166718PurposesgRNA against human BAP1DepositorAvailable SinceJune 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGMC00002
Plasmid#166719PurposesgRNA against human BAP1DepositorAvailable SinceJune 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-dCas9-p300 Core
Plasmid#61357Purposeencodes S. pyogenes dCas9 with c-terminal fusion of human p300 HAT core (aa 1048-1664) driven by CMV promoterDepositorInsertS.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (EP300 S. pyogenes, Human, Synthetic)
UseCRISPR and Synthetic BiologyTagsFlag, HA, and SV40 NLSExpressionMammalianMutationD10A; H840A in S.pyogenes Cas9PromoterCMVAvailable SinceApril 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-dCas9-p300 Core (D1399Y)
Plasmid#61358Purposeencodes S. pyogenes dCas9 with c-terminal fusion of human p300 HAT core (aa 1048-1664; inactivating mutation D1399Y) driven by CMV promoterDepositorInsertS.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (EP300 S. pyogenes, Human, Synthetic)
UseCRISPR and Synthetic BiologyTagsFlag, HA, and SV40 NLSExpressionMammalianMutationD10A; H840A in S.pyogenes Cas9; D1399Y mutation i…PromoterCMVAvailable SinceApril 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-dCas9-p300 Core (Y1467F)
Plasmid#61362Purposeencodes S. pyogenes dCas9 with c-terminal fusion of human p300 HAT core (aa 1048-1664; inavtivating mutation Y1467F) driven by CMV promoterDepositorInsertS.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (EP300 S. pyogenes, Human, Synthetic)
UseCRISPR and Synthetic BiologyTagsFlag, HA, and SV40 NLSExpressionMammalianMutationD10A; H840A in S.pyogenes Cas9; Y1467F mutation i…PromoterCMVAvailable SinceApril 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBabe-Puro-BRAF-V600E
Plasmid#15269PurposeRetroviral expression of BRAF V600E (the V600E mutation has been found in cancer patients, confers increased kinase activity, and is transforming)DepositorInsertBRAF (BRAF Human)
UseRetroviralTagsHis and MycExpressionMammalianMutationValine 600 to Glutamate (mutation has been found …Available SinceJuly 12, 2007AvailabilityAcademic Institutions and Nonprofits only -
LeGO-iG-CSF2RB
Plasmid#125750PurposeLentiviral Expression Vektor with CSF2RB wildtype cDNA and IRES eGFPDepositorAvailable SinceJuly 12, 2019AvailabilityAcademic Institutions and Nonprofits only