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pCMV-dLbCpf1-BE-YE1

Plasmid

#154145

Purpose

Expresses dLbCpf1-BE-YE1(dLbCas12a-BE-YE1) in mammalian cells.

Depositor

Jin-Soo Kim

Article

Kim et al Nat Commun. 2020 Aug 13;11(1):4072. doi:

Insert

dLbCpf1-BE-YE1 (dCas12a-BE-YE1)

Tags

SV40 NLS

Expression

Mammalian

Mutation

APOBEC1(W90Y+R126E), dLbCpf1(D832A+E925A+D1148A)

Promoter

CMV

Available Since

Aug. 24, 2022

Availability

Academic Institutions and Nonprofits only

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REM2

Plasmid

#55682

Purpose

expression of mouse REM2

Depositor

Julian Downward

Article

Fritsch et al Cell. 2013 May 23;153(5):1050-63. doi: 1

Insert

REM2 (Rem2 Mouse)

Expression

Bacterial

Promoter

lac

Available Since

Sept. 30, 2014

Availability

Academic Institutions and Nonprofits only
pDRF1 mScarlet1.4-T2A-mTurquoise2

Plasmid

#219849

Purpose

equimolar expression of mScarlet1.4 and mTurquoise2 in budding yeast

Depositor

Bas Teusink

Article

Botman et al ACS Synth Biol. 2026 Apr 1. doi: 10.1021

Insert

mScarlet1.4-T2A-mTurquoise2

Expression

Yeast

Available Since

Aug. 27, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pDRF1 mStayGold-T2A-mCherry

Plasmid

#219834

Purpose

equimolar expression of mStaygold and mCherry in budding yeast

Depositor

Bas Teusink

Article

Botman et al ACS Synth Biol. 2026 Apr 1. doi: 10.1021

Insert

mStayGold-T2A-mCherry

Expression

Yeast

Available Since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pDRF1 Cyterm-ytd8oxStayGold

Plasmid

#219853

Purpose

expression of Cyterm-ytd8oxStayGold in budding yeast

Depositor

Bas Teusink

Article

Botman et al ACS Synth Biol. 2026 Apr 1. doi: 10.1021

Insert

Cyterm-ytd8oxStayGold

Expression

Yeast

Available Since

Aug. 27, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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RIT1

Plasmid

#55662

Purpose

expression of mouse RIT1

Depositor

Julian Downward

Article

Fritsch et al Cell. 2013 May 23;153(5):1050-63. doi: 1

Insert

RIT1 (Rit1 Mouse)

Expression

Bacterial

Promoter

lac

Available Since

Aug. 21, 2014

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1-hRabin8 F201A

Plasmid

#26728

Depositor

Keith Mostov

Article

Bryant et al Nat Cell Biol. 2010 Nov;12(11):1035-45.

Insert

Rabin8 (RAB3IP Human)

Tags

EGFP

Expression

Mammalian

Mutation

F201A: GEF domain mutant

Available Since

Dec. 8, 2010

Availability

Academic Institutions and Nonprofits only
RASD1

Plasmid

#55671

Purpose

expression of mouse RASD1

Depositor

Julian Downward

Article

Fritsch et al Cell. 2013 May 23;153(5):1050-63. doi: 1

Insert

RASD1 (Rasd1 Mouse)

Expression

Bacterial

Promoter

lac

Available Since

Aug. 21, 2014

Availability

Academic Institutions and Nonprofits only
pDRF1 ymStayGold-T2A-mCherry

Plasmid

#219843

Purpose

equimolar expression of ymStayGold and mCherry in budding yeast

Depositor

Bas Teusink

Article

Botman et al ACS Synth Biol. 2026 Apr 1. doi: 10.1021

Insert

ymStayGold-T2A-mCherry

Expression

Yeast

Available Since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1-hRabin8 L196A

Plasmid

#26727

Depositor

Keith Mostov

Article

Bryant et al Nat Cell Biol. 2010 Nov;12(11):1035-45.

Insert

Rabin8 (RAB3IP Human)

Tags

EGFP

Expression

Mammalian

Mutation

L196A: GEF domain mutant.

Available Since

Dec. 8, 2010

Availability

Academic Institutions and Nonprofits only
pBT138b-pro1

Plasmid

#138513

Purpose

P3h

Depositor

David Liu

Article

Richter et al Nat Biotechnol. 2020 Jul;38(7):883-891.

Insert

T7-RNAP

Use

Synthetic Biology

Available Since

March 16, 2020

Availability

Academic Institutions and Nonprofits only

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Str-KDEL_flGalT-SBP-tagBFP

Plasmid

#65274

Purpose

synchronize trafficking of GalT from the ER (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

galactosidase

Tags

Streptavidin Binding Protein (SBP) and tagBFP

Expression

Mammalian

Promoter

CMV

Available Since

June 12, 2015

Availability

Academic Institutions and Nonprofits only

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SBP-mCherry-Ecadherin_puromycin

Plasmid

#65293

Purpose

Ecadherin reporter only (no hook) (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

Ecadherin

Tags

IL-2 Signal Sequence and Streptavidin Binding Pro…

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 3, 2015

Availability

Academic Institutions and Nonprofits only

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pCMV-deltaR8.2(A14C,E45C,A92E)

Plasmid

#79048

Purpose

mammalian expression, lentiviral packaging

Depositor

Wesley Sundquist

Article

Li et al Elife. 2016 Jun 2;5. pii: e16269. doi: 1

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

March 20, 2017

Availability

Academic Institutions and Nonprofits only

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pET22-HT/prsA

Plasmid

#73624

Purpose

Produces Escherichia coli phosphoribosylpyrophosphate synthetase (PrsA), N-terminal His6 tag

Depositor

T. J. Kappock, James Williamson

Article

Schultheisz et al ACS Chem Biol. 2008 Aug 15;3(8):499-511.

Insert

ribose-phosphate diphosphokinase (prs Escherichia coli str. K-12 substr. MG1655)

Tags

His6

Expression

Bacterial

Promoter

T7

Available Since

Feb. 22, 2016

Availability

Academic Institutions and Nonprofits only

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pRS416-Aga1p-FAPB2.3.6L1TAG

Plasmid

#158148

Purpose

Yeast display reporter for non-display yeast strains (TAG construct)

Depositor

James Van Deventer

Article

Potts et al Mol Syst Des Eng. 2020 Feb 1;5(2):573-58

Insert

Aga1p-FAPB2.3.6

Expression

Yeast

Mutation

Cloned in a TAG codon at the first position of th…

Promoter

GAL1-10

Available Since

Jan. 12, 2021

Availability

Academic Institutions and Nonprofits only

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MT21-Ubc9

Plasmid

#17273

Depositor

Stephen Goff

Article

Yueh et al J Virol. 2006 Jan . 80(1):342-52.

Insert

Ubc9 (Ube2i Mouse)

Expression

Mammalian

Available Since

March 1, 2010

Availability

Academic Institutions and Nonprofits only
pMSCV-PIG-miR-30b

Plasmid

#64232

Purpose

Expresses miR-30b in mammalian cells

Depositor

Joshua Mendell

Article

Chang et al Nat Genet. 2008 Jan;40(1):43-50. Epub 20

Insert

miR-30b (MIR30B Human)

Use

Retroviral

Expression

Mammalian

Available Since

June 2, 2015

Availability

Academic Institutions and Nonprofits only

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FLAG TBC1D13

Plasmid

#87953

Purpose

Expresses flag tagged mouse TBC1D13

Depositor

David James

Article

Davey et al Traffic. 2012 Oct;13(10):1429-41. doi: 1

Insert

TBC1D13 (Tbc1d13 Mouse)

Tags

FLAG

Expression

Mammalian

Available Since

May 30, 2017

Availability

Academic Institutions and Nonprofits only

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pBT138b-proA

Plasmid

#138514

Purpose

P3g

Depositor

David Liu

Article

Richter et al Nat Biotechnol. 2020 Jul;38(7):883-891.

Insert

T7-RNAP

Use

Synthetic Biology

Available Since

March 16, 2020

Availability

Academic Institutions and Nonprofits only

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